Examples with VCFHeader htsjdk.variant.vcf.VCFHeader used on opensource projects

Example 56 with VCFHeader

use of htsjdk.variant.vcf.VCFHeader in project jvarkit by lindenb.

the class VcfStatsJfx method doWork.

@Override
protected int doWork(final Stage primaryStage, final List<String> args) {
    try {
        if (args.size() != 1) {
            System.err.println("VCF missing");
            return -1;
        }
        final VCFFileReader vcfFileReader = new VCFFileReader(new File(args.get(0)));
        final VCFHeader header = vcfFileReader.getFileHeader();
        chartGenerators.add(new VariantTypeGenerator());
        if (!header.getFilterLines().isEmpty()) {
            chartGenerators.add(new FilterUsageGenerator(header.getFilterLines()));
        }
        final SAMSequenceDictionary dict = header.getSequenceDictionary();
        if (dict != null && !dict.isEmpty()) {
            chartGenerators.add(new ContigUsageGenerator(dict));
        }
        if (header.hasGenotypingData()) {
            chartGenerators.add(new GenotypeTypeGenerator(header.getGenotypeSamples()));
            chartGenerators.add(new AffectedSamplesGenerator());
            for (final String sn : header.getSampleNamesInOrder()) {
            }
        }
        final VariantContextRunner runner = new VariantContextRunner(vcfFileReader);
        primaryStage.setOnShowing(AE -> {
            new Thread(runner).start();
        });
        primaryStage.setOnCloseRequest(AE -> {
            stop = true;
            CloserUtil.close(runner);
        });
        final TabPane tabPane = new TabPane();
        chartGenerators.removeIf(G -> !G.isEnabled());
        for (final ChartGenerator g : chartGenerators) {
            tabPane.getTabs().add(g.makeTab());
        }
        final Screen scr = Screen.getPrimary();
        Scene scene = new Scene(tabPane, scr.getBounds().getWidth() - 100, scr.getBounds().getHeight() - 100);
        primaryStage.setScene(scene);
        primaryStage.show();
    } catch (final Exception err) {
        err.printStackTrace();
        return -1;
    } finally {
    }
    return 0;
}

Example 57 with VCFHeader

use of htsjdk.variant.vcf.VCFHeader in project jvarkit by lindenb.

the class VcfUcsc method doVcfToVcf.

@Override
protected int doVcfToVcf(final String inputName, final VcfIterator in, final VariantContextWriter out) {
    final String TAG;
    if (StringUtil.isBlank(this.infoTag)) {
        TAG = "UCSC_" + database.toUpperCase() + "_" + table.toUpperCase();
    } else {
        TAG = this.infoTag;
    }
    VCFHeader header = in.getHeader();
    final VCFHeader h2 = new VCFHeader(header);
    h2.addMetaDataLine(new VCFInfoHeaderLine(TAG, VCFHeaderLineCount.UNBOUNDED, VCFHeaderLineType.String, database + "." + table));
    if (!StringUtil.isBlank(this.filterIn)) {
        h2.addMetaDataLine(new VCFFilterHeaderLine(this.filterIn, "Set by " + this.getClass().getName()));
    } else if (!StringUtil.isBlank(this.filterOut)) {
        h2.addMetaDataLine(new VCFFilterHeaderLine(this.filterOut, "Set by " + this.getClass().getName()));
    }
    h2.addMetaDataLine(new VCFHeaderLine(getClass().getSimpleName() + "CmdLine", String.valueOf(getProgramCommandLine())));
    h2.addMetaDataLine(new VCFHeaderLine(getClass().getSimpleName() + "Version", String.valueOf(getVersion())));
    out.writeHeader(h2);
    final Map<Integer, PreparedStatement> bin2pstmt = new HashMap<>();
    try {
        if (!this.has_bin_column) {
            bin2pstmt.put(0, createPreparedStatement(0));
        }
        final SAMSequenceDictionaryProgress progress = new SAMSequenceDictionaryProgress(header).logger(LOG);
        while (in.hasNext()) {
            final VariantContext ctx = progress.watch(in.next());
            int start0, end0;
            if (// mutation starts *after* the base
            ctx.isIndel()) {
                start0 = ctx.getStart();
                end0 = ctx.getEnd();
            } else {
                start0 = ctx.getStart() - 1;
                end0 = ctx.getEnd();
            }
            // extends left/right
            start0 = Math.max(0, start0 - this.extend_bases);
            end0 += this.extend_bases;
            final Set<String> atts = new HashSet<String>();
            if (this.has_bin_column) {
                final List<Integer> binList = reg2bins(start0, end0);
                PreparedStatement pstmt = bin2pstmt.get(binList.size());
                if (pstmt == null) {
                    LOG.debug("create prepared statemement for bin.size=" + binList.size() + "[" + start0 + ":" + end0 + "]");
                    pstmt = createPreparedStatement(binList.size());
                    bin2pstmt.put(binList.size(), pstmt);
                }
                initPstmt(pstmt, ctx.getContig(), start0, end0);
                for (int x = 0; x < binList.size(); ++x) {
                    pstmt.setInt(4 + x, binList.get(x));
                }
                select(atts, pstmt);
            } else {
                // already defined
                final PreparedStatement pstmt = bin2pstmt.get(0);
                initPstmt(pstmt, ctx.getContig(), start0, end0);
                select(atts, pstmt);
            }
            if (atts.isEmpty() && StringUtil.isBlank(this.filterIn) && StringUtil.isBlank(this.filterOut)) {
                out.add(ctx);
                continue;
            }
            final VariantContextBuilder vcb = new VariantContextBuilder(ctx);
            if (!StringUtil.isBlank(this.filterIn) && !atts.isEmpty()) {
                vcb.filter(this.filterIn);
            } else if (!StringUtil.isBlank(this.filterOut) && atts.isEmpty()) {
                vcb.filter(this.filterOut);
            }
            if (!atts.isEmpty()) {
                vcb.attribute(TAG, atts.toArray());
            }
            out.add(vcb.make());
        }
        progress.finish();
        return 0;
    } catch (final SQLException err) {
        LOG.error(err);
        throw new RuntimeException("SQLError", err);
    } finally {
        for (final PreparedStatement pstmt : bin2pstmt.values()) {
            CloserUtil.close(pstmt);
        }
        bin2pstmt.clear();
    }
}

Example 58 with VCFHeader

use of htsjdk.variant.vcf.VCFHeader in project jvarkit by lindenb.

the class VCFFixIndels method doVcfToVcf.

@Override
protected int doVcfToVcf(String inputName, VcfIterator r, VariantContextWriter w) {
    long nChanged = 0L;
    final String TAG = "INDELFIXED";
    final VCFHeader header = r.getHeader();
    final VCFHeader h2 = new VCFHeader(header);
    addMetaData(h2);
    h2.addMetaDataLine(new VCFInfoHeaderLine(TAG, 1, VCFHeaderLineType.String, "Fix Indels for @SolenaLS (position|alleles...)"));
    w.writeHeader(h2);
    final SAMSequenceDictionaryProgress progress = new SAMSequenceDictionaryProgress(header);
    while (r.hasNext()) {
        boolean somethingChanged = false;
        VariantContext ctx = progress.watch(r.next());
        /* map old allele to new allele */
        Map<Allele, Allele> original2modified = new HashMap<Allele, Allele>();
        /* did we found a strange allele (symbolic, etc ) */
        boolean strange_allele_flag = false;
        for (final Allele a : ctx.getAlleles()) {
            original2modified.put(a, a);
            if (a.isSymbolic() || a.isNoCall()) {
                strange_allele_flag = true;
                break;
            }
        }
        if (strange_allele_flag || original2modified.size() < 2) /* at least 2 alleles: REF+ ALT */
        {
            w.add(ctx);
            continue;
        }
        /* record chromStart if we need to shift to the right */
        int chromStart = ctx.getStart();
        /* trim right then left  */
        for (int side = 0; side < 2; ++side) {
            boolean done = false;
            while (!done) {
                boolean ok_side = true;
                /* common nucleotide at end/start */
                Character targetChar = null;
                done = true;
                // scan side
                Set<Allele> keys = new HashSet<>(original2modified.keySet());
                for (Allele k : keys) {
                    Allele newAllele = original2modified.get(k);
                    if (newAllele.isSymbolic()) {
                        ok_side = false;
                        break;
                    }
                    String baseString = newAllele.getBaseString().trim().toUpperCase();
                    if (baseString.length() < 2) {
                        ok_side = false;
                        break;
                    }
                    /* first or last char or all sequences
						 * side==0 : right
						 * side==1 : left
						 * */
                    Character baseChar = (side == 0 ? baseString.charAt(baseString.length() - 1) : baseString.charAt(0));
                    if (targetChar == null) {
                        targetChar = baseChar;
                    } else if (!targetChar.equals(baseChar)) {
                        /* doesn't end with same nucleotide */
                        ok_side = false;
                        break;
                    }
                }
                /* ok we can shift all alleles */
                if (ok_side && targetChar != null) {
                    done = false;
                    somethingChanged = true;
                    for (Allele k : keys) {
                        Allele newAllele = original2modified.get(k);
                        String baseString = newAllele.getBaseString().trim().toUpperCase();
                        if (// remove last nucleotide
                        side == 0) {
                            newAllele = Allele.create(baseString.substring(0, baseString.length() - 1), newAllele.isReference());
                        } else {
                            newAllele = Allele.create(baseString.substring(1), newAllele.isReference());
                        }
                        original2modified.put(k, newAllele);
                    }
                    if (side == 1)
                        chromStart++;
                }
            }
        /* end of while done */
        }
        if (!somethingChanged) {
            w.add(ctx);
            continue;
        }
        final VariantContextBuilder b = new VariantContextBuilder(ctx);
        b.start(chromStart);
        Allele newRef = original2modified.get(ctx.getReference());
        b.stop(chromStart + newRef.getBaseString().length() - 1);
        b.alleles(original2modified.values());
        List<Genotype> genotypes = new ArrayList<>();
        for (final String sample : header.getSampleNamesInOrder()) {
            final Genotype g = ctx.getGenotype(sample);
            if (!g.isCalled()) {
                genotypes.add(g);
                continue;
            }
            final GenotypeBuilder gb = new GenotypeBuilder(g);
            final List<Allele> aL = new ArrayList<>();
            for (final Allele a : g.getAlleles()) {
                aL.add(original2modified.get(a));
            }
            gb.alleles(aL);
            genotypes.add(gb.make());
        }
        StringBuilder tagContent = new StringBuilder();
        tagContent.append(String.valueOf(ctx.getStart()));
        for (Allele a : ctx.getAlleles()) {
            tagContent.append("|");
            tagContent.append(a.toString());
        }
        b.attribute(TAG, tagContent.toString());
        b.genotypes(genotypes);
        w.add(b.make());
        ++nChanged;
        if (w.checkError())
            break;
    }
    progress.finish();
    LOG.info("indels changed:" + nChanged);
    return RETURN_OK;
}

Example 59 with VCFHeader

use of htsjdk.variant.vcf.VCFHeader in project jvarkit by lindenb.

the class VcfOffsetsIndexFactory method indexVcfFile.

/**
 * index a vcf file for its variant offsets
 */
public File indexVcfFile(final File vcfFile, final File indexFile) throws IOException {
    LOG.info("indexing " + vcfFile);
    IOUtil.assertFileIsReadable(vcfFile);
    DataOutputStream daos = null;
    BlockCompressedInputStream bgzin = null;
    AsciiLineReader ascii = null;
    VCFHeader header = null;
    final VCFCodec codec = new VCFCodec();
    SAMSequenceDictionaryProgress progress = null;
    try {
        daos = new DataOutputStream(new FileOutputStream(indexFile));
        daos.write(MAGIC);
        if (vcfFile.getName().endsWith(".vcf.gz")) {
            bgzin = new BlockCompressedInputStream(vcfFile);
            ascii = null;
        } else if (vcfFile.getName().endsWith(".vcf")) {
            bgzin = null;
            ascii = new AsciiLineReader(new FileInputStream(vcfFile));
        } else {
            throw new IllegalArgumentException("not a vcf.gz or vcf file: " + vcfFile);
        }
        final List<String> headerLines = new ArrayList<>();
        for (; ; ) {
            final long offset = (ascii == null ? bgzin.getPosition() : ascii.getPosition());
            final String line = (ascii == null ? bgzin.readLine() : ascii.readLine());
            if (line == null)
                break;
            if (line.startsWith("#")) {
                headerLines.add(line);
                if (line.startsWith("#CHROM")) {
                    codec.readHeader(new LineIterator() {

                        int i = 0;

                        @Override
                        public String next() {
                            final String s = headerLines.get(i);
                            i++;
                            return s;
                        }

                        @Override
                        public boolean hasNext() {
                            return i < headerLines.size();
                        }

                        @Override
                        public String peek() {
                            return i < headerLines.size() ? headerLines.get(i) : null;
                        }
                    });
                    header = VCFUtils.parseHeader(headerLines).header;
                    progress = new SAMSequenceDictionaryProgress(header);
                    progress.logger(this.logger == null ? LOG : this.logger);
                    progress.setLogPrefix("indexing");
                }
                continue;
            }
            if (progress == null) {
                throw new JvarkitException.FileFormatError("no vcf header in " + vcfFile);
            }
            final VariantContext ctx = codec.decode(line);
            progress.watch(ctx);
            if (this.acceptVariant != null) {
                if (!acceptVariant.test(ctx))
                    continue;
            }
            daos.writeLong(offset);
        }
        if (progress == null) {
            throw new JvarkitException.FileFormatError("no vcf header in " + vcfFile);
        }
        progress.finish();
        daos.flush();
        daos.close();
        return indexFile;
    } catch (final IOException err) {
        throw err;
    } finally {
        CloserUtil.close(ascii);
        CloserUtil.close(bgzin);
        CloserUtil.close(daos);
    }
}

Example 60 with VCFHeader

use of htsjdk.variant.vcf.VCFHeader in project jvarkit by lindenb.

the class VCFComm method doWork.

@Override
public int doWork(final List<String> args) {
    CloseableIterator<LineAndFile> iter = null;
    SortingCollection<LineAndFile> variants = null;
    VariantContextWriter w = null;
    try {
        if (args.isEmpty()) {
            LOG.error("Illegal number of arguments");
            return -1;
        }
        Set<VCFHeaderLine> metaData = new HashSet<VCFHeaderLine>();
        variants = SortingCollection.newInstance(LineAndFile.class, new LineAndFileCodec(), new LineAndFileComparator(), super.sortingCollectionArgs.getMaxRecordsInRam(), super.sortingCollectionArgs.getTmpPaths());
        variants.setDestructiveIteration(true);
        /**
         * new sample names in the output vcf: one  sample per file
         */
        final Map<Integer, String> fileid2sampleName = new TreeMap<>();
        /**
         * samples names as they appear in the original VCF headers
         */
        final Counter<String> countInputSamples = new Counter<String>();
        /**
         * dicts
         */
        final List<SAMSequenceDictionary> all_dictionaries = new ArrayList<>();
        for (final String vcffilename : IOUtils.unrollFiles(args)) {
            LOG.info("Reading from " + vcffilename);
            final Input input = super.put(variants, vcffilename);
            String sampleName = vcffilename;
            if (sampleName.endsWith(".vcf.gz")) {
                sampleName = sampleName.substring(0, sampleName.length() - 7);
            } else if (sampleName.endsWith(".vcf.gz")) {
                sampleName = sampleName.substring(0, sampleName.length() - 4);
            }
            int slash = sampleName.lastIndexOf(File.separatorChar);
            if (slash != -1)
                sampleName = sampleName.substring(slash + 1);
            int suffix = 1;
            // loop until we find a uniq name
            for (; ; ) {
                final String key = sampleName + (suffix == 1 ? "" : "_" + suffix);
                if (fileid2sampleName.values().contains(key)) {
                    suffix++;
                    continue;
                }
                fileid2sampleName.put(input.file_id, key);
                metaData.add(new VCFHeaderLine(key, vcffilename));
                break;
            }
            for (final String sname : input.codecAndHeader.header.getSampleNamesInOrder()) {
                countInputSamples.incr(sname);
            }
            all_dictionaries.add(input.codecAndHeader.header.getSequenceDictionary());
        }
        variants.doneAdding();
        /**
         * unique sample name, if any present in all VCF
         */
        Optional<String> unqueSampleName = Optional.empty();
        if (countInputSamples.getCountCategories() == 1 && countInputSamples.count(countInputSamples.keySet().iterator().next()) == fileid2sampleName.size()) {
            unqueSampleName = Optional.of(countInputSamples.keySet().iterator().next());
            LOG.info("Unique sample name is " + unqueSampleName.get());
        }
        VCFStandardHeaderLines.addStandardFormatLines(metaData, true, VCFConstants.DEPTH_KEY, VCFConstants.GENOTYPE_QUALITY_KEY, VCFConstants.GENOTYPE_KEY, VCFConstants.GENOTYPE_FILTER_KEY);
        VCFStandardHeaderLines.addStandardInfoLines(metaData, true, VCFConstants.DEPTH_KEY, VCFConstants.ALLELE_COUNT_KEY, VCFConstants.ALLELE_NUMBER_KEY);
        metaData.add(new VCFHeaderLine(getClass().getSimpleName(), "version:" + getVersion() + " command:" + getProgramCommandLine()));
        final VCFFilterHeaderLine variantNotCalledInAllVcf = new VCFFilterHeaderLine("NotCalledEveryWhere", "Variant was NOT called in all input VCF");
        metaData.add(variantNotCalledInAllVcf);
        final VCFFilterHeaderLine variantWasFiltered = new VCFFilterHeaderLine("VariantWasFiltered", "At least one variant was filtered");
        metaData.add(variantWasFiltered);
        final VCFFormatHeaderLine variantQUALFormat = new VCFFormatHeaderLine("VCQUAL", 1, VCFHeaderLineType.Float, "Variant Quality");
        metaData.add(variantQUALFormat);
        metaData.add(new VCFFormatHeaderLine(VCFConstants.ALLELE_NUMBER_KEY, 1, VCFHeaderLineType.Integer, "Number of allle in the src vcf"));
        metaData.add(new VCFFormatHeaderLine(VCFConstants.ALLELE_COUNT_KEY, 1, VCFHeaderLineType.Integer, "Number of ALT alllele"));
        final VCFInfoHeaderLine foundInCountVcfInfo = new VCFInfoHeaderLine("NVCF", 1, VCFHeaderLineType.Integer, "Number of VCF this variant was found");
        metaData.add(foundInCountVcfInfo);
        final VCFInfoHeaderLine variantTypesInfo = new VCFInfoHeaderLine("VTYPES", VCFHeaderLineCount.UNBOUNDED, VCFHeaderLineType.String, "Distinct Variants type");
        metaData.add(variantTypesInfo);
        final VCFFilterHeaderLine multipleTypeFilters = new VCFFilterHeaderLine("DiscordantTypes", "Discordant types at this position");
        metaData.add(multipleTypeFilters);
        final VCFFormatHeaderLine variantTypeFormat = new VCFFormatHeaderLine("VTYPE", 1, VCFHeaderLineType.String, "Variant Type");
        metaData.add(variantTypeFormat);
        final VCFFilterHeaderLine uniqueVariantDiscordantGTFilter;
        if (unqueSampleName.isPresent()) {
            metaData.add(new VCFHeaderLine("UniqSample", unqueSampleName.get()));
            uniqueVariantDiscordantGTFilter = new VCFFilterHeaderLine("DiscordantGenotypeForUniqSample", "Genotype Dicordant for for sample " + unqueSampleName.get());
            metaData.add(uniqueVariantDiscordantGTFilter);
        } else {
            uniqueVariantDiscordantGTFilter = null;
        }
        final VCFHeader header = new VCFHeader(metaData, new ArrayList<>(fileid2sampleName.values()));
        if (// all have a dict
        !normalize_chr && !all_dictionaries.contains(null)) {
            SAMSequenceDictionary thedict = null;
            for (int x = 0; x < all_dictionaries.size(); ++x) {
                SAMSequenceDictionary d = all_dictionaries.get(x);
                if (thedict == null) {
                    thedict = d;
                } else if (!SequenceUtil.areSequenceDictionariesEqual(d, thedict)) {
                    thedict = null;
                    break;
                }
            }
            if (thedict != null)
                header.setSequenceDictionary(thedict);
        }
        w = super.openVariantContextWriter(super.outputFile);
        w.writeHeader(header);
        final List<LineAndFile> row = new ArrayList<LineAndFile>(super.inputs.size());
        final Comparator<LineAndFile> posCompare = (A, B) -> A.getContigPosRef().compareTo(B.getContigPosRef());
        iter = variants.iterator();
        for (; ; ) {
            LineAndFile rec = null;
            if (iter.hasNext()) {
                rec = iter.next();
            }
            if (rec == null || (!row.isEmpty() && posCompare.compare(row.get(0), rec) != 0)) {
                if (!row.isEmpty()) {
                    final VariantContext first = row.get(0).getContext();
                    /* in which file id we find this variant */
                    Set<Integer> fileids_for_variant = row.stream().map(LAF -> LAF.fileIdx).collect(Collectors.toSet());
                    // see with HAS multiple chrom/pos/ref but different alt
                    if (row.size() != fileids_for_variant.size()) {
                        for (; ; ) {
                            boolean ok = true;
                            for (int x = 0; ok && x + 1 < row.size(); ++x) {
                                final VariantContext ctxx = row.get(x).getContext();
                                final List<Allele> altsx = ctxx.getAlternateAlleles();
                                for (int y = x + 1; ok && y < row.size(); ++y) {
                                    if (row.get(x).fileIdx != row.get(y).fileIdx)
                                        continue;
                                    final VariantContext ctxy = row.get(y).getContext();
                                    final List<Allele> altsy = ctxy.getAlternateAlleles();
                                    if (altsx.equals(altsy))
                                        continue;
                                    if (!ctxx.isVariant() && ctxy.isVariant()) {
                                        row.remove(x);
                                    } else if (ctxx.isVariant() && !ctxy.isVariant()) {
                                        row.remove(y);
                                    } else if (!ctxx.isSNP() && ctxy.isSNP()) {
                                        row.remove(x);
                                    } else if (ctxx.isSNP() && !ctxy.isSNP()) {
                                        row.remove(y);
                                    } else if (altsx.size() > altsy.size()) {
                                        row.remove(x);
                                    } else if (altsx.size() < altsy.size()) {
                                        row.remove(y);
                                    } else {
                                        row.remove(y);
                                    }
                                    ok = false;
                                    break;
                                }
                            }
                            if (ok)
                                break;
                        }
                        fileids_for_variant = row.stream().map(LAF -> LAF.fileIdx).collect(Collectors.toSet());
                    }
                    if (row.size() != fileids_for_variant.size()) {
                        LOG.error("There are some duplicated variants at the position " + new ContigPosRef(first) + " in the same vcf file");
                        for (final LineAndFile laf : row) {
                            LOG.error("File [" + laf.fileIdx + "]" + fileid2sampleName.get(laf.fileIdx));
                            LOG.error("\t" + laf.getContigPosRef());
                        }
                        row.clear();
                    } else {
                        final Set<Allele> alleles = row.stream().flatMap(R -> R.getContext().getAlleles().stream()).collect(Collectors.toSet());
                        final VariantContextBuilder vcb = new VariantContextBuilder(getClass().getName(), first.getContig(), first.getStart(), first.getEnd(), alleles);
                        final Set<String> filters = new HashSet<>();
                        final Set<VariantContext.Type> variantContextTypes = new HashSet<>();
                        final List<Genotype> genotypes = new ArrayList<Genotype>();
                        for (final LineAndFile laf : row) {
                            if (laf.getContext().isFiltered())
                                filters.add(variantWasFiltered.getID());
                            variantContextTypes.add(laf.getContext().getType());
                            final GenotypeBuilder gbuilder = new GenotypeBuilder();
                            gbuilder.name(fileid2sampleName.get(laf.fileIdx));
                            if (unqueSampleName.isPresent()) {
                                final Genotype g0 = laf.getContext().getGenotype(unqueSampleName.get());
                                if (g0 == null) {
                                    iter.close();
                                    w.close();
                                    throw new IllegalStateException("Cannot find genotype for " + unqueSampleName.get());
                                }
                                if (g0.hasDP())
                                    gbuilder.DP(g0.getDP());
                                if (g0.hasGQ())
                                    gbuilder.GQ(g0.getGQ());
                                gbuilder.alleles(g0.getAlleles());
                            } else {
                                gbuilder.alleles(Arrays.asList(first.getReference(), first.getReference()));
                                if (laf.getContext().hasAttribute(VCFConstants.DEPTH_KEY)) {
                                    gbuilder.DP(laf.getContext().getAttributeAsInt(VCFConstants.DEPTH_KEY, 0));
                                }
                            }
                            if (laf.getContext().isFiltered()) {
                                gbuilder.filter("VCFFILTERED");
                            }
                            if (laf.getContext().hasLog10PError()) {
                                gbuilder.attribute(variantQUALFormat.getID(), laf.getContext().getPhredScaledQual());
                            }
                            gbuilder.attribute(VCFConstants.ALLELE_NUMBER_KEY, laf.getContext().getGenotypes().stream().flatMap(G -> G.getAlleles().stream()).filter(A -> !A.isNoCall()).count());
                            gbuilder.attribute(VCFConstants.ALLELE_COUNT_KEY, laf.getContext().getGenotypes().stream().flatMap(G -> G.getAlleles().stream()).filter(A -> !(A.isReference() || A.isNoCall())).count());
                            gbuilder.attribute(variantTypeFormat.getID(), laf.getContext().getType().name());
                            genotypes.add(gbuilder.make());
                        }
                        final String id = String.join(";", row.stream().map(LAF -> LAF.getContext()).filter(V -> V.hasID()).map(V -> V.getID()).collect(Collectors.toSet()));
                        if (!id.isEmpty())
                            vcb.id(id);
                        vcb.genotypes(genotypes);
                        if (unqueSampleName.isPresent()) {
                            boolean all_same = true;
                            for (int x = 0; all_same && x + 1 < genotypes.size(); ++x) {
                                if (!genotypes.get(x).isCalled())
                                    continue;
                                for (int y = x + 1; all_same && y < genotypes.size(); ++y) {
                                    if (!genotypes.get(y).isCalled())
                                        continue;
                                    if (!genotypes.get(x).sameGenotype(genotypes.get(y), true)) {
                                        all_same = false;
                                        break;
                                    }
                                }
                            }
                            if (!all_same)
                                filters.add(uniqueVariantDiscordantGTFilter.getID());
                        }
                        // Add AN
                        vcb.attribute(VCFConstants.ALLELE_NUMBER_KEY, genotypes.stream().filter(G -> G.isCalled()).mapToInt(G -> G.getAlleles().size()).sum());
                        if (!variantContextTypes.isEmpty()) {
                            vcb.attribute(variantTypesInfo.getID(), new ArrayList<>(variantContextTypes.stream().map(T -> T.name()).collect(Collectors.toSet())));
                            if (variantContextTypes.size() > 1) {
                                filters.add(multipleTypeFilters.getID());
                            }
                        }
                        vcb.attribute(foundInCountVcfInfo.getID(), fileids_for_variant.size());
                        boolean print = true;
                        if (row.size() == super.inputs.size() && ignore_everywhere) {
                            print = false;
                        }
                        if (fileids_for_variant.size() != fileid2sampleName.size()) {
                            filters.add(variantNotCalledInAllVcf.getID());
                            if (only_everywhere) {
                                print = false;
                            }
                        }
                        vcb.filters(filters);
                        if (print) {
                            w.add(vcb.make());
                        }
                    }
                    row.clear();
                }
                if (rec == null)
                    break;
            }
            row.add(rec);
        }
        iter.close();
        iter = null;
        w.close();
        w = null;
        return 0;
    } catch (Exception err) {
        LOG.error(err);
        return -1;
    } finally {
        CloserUtil.close(iter);
        CloserUtil.close(w);
        try {
            if (variants != null)
                variants.cleanup();
        } catch (Exception err) {
        }
    }
}