use of ij.gui.GenericDialog in project GDSC-SMLM by aherbert.
the class EMGainAnalysis method showDialog.
private int showDialog() {
GenericDialog gd = new GenericDialog(TITLE);
gd.addHelp(About.HELP_URL);
gd.addMessage("Analyse the white-light histogram of an image stack to determine EM-gain parameters.\n \n" + "See Ulbrich & Isacoff (2007). Nature Methods 4, 319-321 (Supplementary Information).");
if (extraOptions) {
gd.addCheckbox("Simulate", _simulate);
gd.addNumericField("Bias", _bias, 0);
gd.addNumericField("Gain", _gain, 2);
gd.addNumericField("Noise", _noise, 2);
gd.addNumericField("Photons", _photons, 2);
gd.addNumericField("Samples", simulationSize, 0);
gd.addCheckbox("Sample_PDF", usePDF);
}
gd.addNumericField("Bias_estimate", bias, 0);
gd.addNumericField("Gain_estimate", gain, 2);
gd.addNumericField("Noise_estimate", noise, 2);
gd.addCheckbox("Show_approximation", showApproximation);
gd.showDialog();
if (gd.wasCanceled())
return DONE;
if (extraOptions) {
simulate = _simulate = gd.getNextBoolean();
_bias = gd.getNextNumber();
_gain = gd.getNextNumber();
_noise = FastMath.abs(gd.getNextNumber());
_photons = FastMath.abs(gd.getNextNumber());
simulationSize = (int) FastMath.abs(gd.getNextNumber());
usePDF = gd.getNextBoolean();
if (gd.invalidNumber() || _bias < 0 || _gain < 1 || _photons == 0 || simulationSize == 0)
return DONE;
}
bias = gd.getNextNumber();
gain = gd.getNextNumber();
noise = FastMath.abs(gd.getNextNumber());
showApproximation = gd.getNextBoolean();
if (gd.invalidNumber() || bias < 0 || gain < 1)
return DONE;
return (simulate) ? NO_IMAGE_REQUIRED : FLAGS;
}
use of ij.gui.GenericDialog in project GDSC-SMLM by aherbert.
the class EMGainAnalysis method showPMFDialog.
private boolean showPMFDialog() {
GenericDialog gd = new GenericDialog(TITLE);
gd.addHelp(About.HELP_URL);
gd.addMessage("Plot the probability mass function for EM-gain");
gd.addNumericField("Gain", _gain, 2);
gd.addNumericField("Noise", _noise, 2);
gd.addNumericField("Photons", _photons, 2);
gd.addChoice("Approx", APPROXIMATION, APPROXIMATION[approximation]);
gd.addCheckbox("Show_approximation", showApproximation);
if (extraOptions)
gd.addNumericField("Approximation_offset (%)", _offset, 2);
gd.addNumericField("Remove_head", head, 3);
gd.addNumericField("Remove_tail", tail, 3);
gd.addCheckbox("Relative_delta", relativeDelta);
gd.showDialog();
if (gd.wasCanceled())
return false;
_gain = gd.getNextNumber();
_noise = FastMath.abs(gd.getNextNumber());
_photons = FastMath.abs(gd.getNextNumber());
approximation = gd.getNextChoiceIndex();
showApproximation = gd.getNextBoolean();
if (extraOptions)
offset = _offset = gd.getNextNumber();
head = FastMath.abs(gd.getNextNumber());
tail = FastMath.abs(gd.getNextNumber());
relativeDelta = gd.getNextBoolean();
if (gd.invalidNumber() || _bias < 0 || _gain < 1 || _photons == 0 || tail > 0.5 || head > 0.5)
return false;
return true;
}
use of ij.gui.GenericDialog in project GDSC-SMLM by aherbert.
the class DoubletAnalysis method showAnalysisDialog.
/**
* Show dialog.
*
* @return true, if successful
*/
@SuppressWarnings("unchecked")
private boolean showAnalysisDialog() {
GenericDialog gd = new GenericDialog(TITLE);
gd.addHelp(About.HELP_URL);
StringBuilder sb = new StringBuilder("Filters the doublet fits and reports the performance increase\n");
// Show the fitting settings that will effect filters, i.e. fit standard deviation, fit width
sb.append("SD0 = ").append(Utils.rounded(fitConfig.getInitialPeakStdDev0())).append("\n");
sb.append("SD1 = ").append(Utils.rounded(fitConfig.getInitialPeakStdDev1())).append("\n");
sb.append("Fit Width = ").append(config.getRelativeFitting()).append("\n");
gd.addMessage(sb.toString());
// Collect options for filtering
gd.addChoice("Selection_Criteria", SELECTION_CRITERIA, SELECTION_CRITERIA[selectionCriteria]);
// Copy the settings used when fitting
filterFitConfig.setInitialPeakStdDev0(fitConfig.getInitialPeakStdDev0());
filterFitConfig.setInitialPeakStdDev1(fitConfig.getInitialPeakStdDev1());
filterFitConfig.setModelCamera(fitConfig.isModelCamera());
filterFitConfig.setNmPerPixel(cal.getNmPerPixel());
filterFitConfig.setGain(cal.getGain());
filterFitConfig.setBias(cal.getBias());
filterFitConfig.setReadNoise(cal.getReadNoise());
filterFitConfig.setAmplification(cal.getAmplification());
filterFitConfig.setEmCCD(cal.isEmCCD());
filterFitConfig.setFitSolver(fitConfig.getFitSolver());
String[] templates = ConfigurationTemplate.getTemplateNames(true);
gd.addChoice("Template", templates, templates[0]);
// Allow the settings from the benchmark analysis to be used
gd.addCheckbox("Benchmark_settings", analysisUseBenchmarkSettings);
gd.addCheckbox("Smart_filter", fitConfig.isSmartFilter());
gd.addSlider("Shift_factor", 0.01, 2, filterFitConfig.getCoordinateShiftFactor());
gd.addNumericField("Signal_strength", filterFitConfig.getSignalStrength(), 2);
gd.addNumericField("Min_photons", filterFitConfig.getMinPhotons(), 0);
gd.addSlider("Min_width_factor", 0, 0.99, filterFitConfig.getMinWidthFactor());
gd.addSlider("Max_width_factor", 1.01, 5, filterFitConfig.getWidthFactor());
gd.addNumericField("Precision", filterFitConfig.getPrecisionThreshold(), 2);
gd.addCheckbox("Local_background", filterFitConfig.isPrecisionUsingBackground());
gd.addNumericField("Drift_angle", analysisDriftAngle, 2);
gd.addNumericField("Min_gap", minGap, 2);
// Collect display options
gd.addCheckbox("Show_results", analysisShowResults);
gd.addCheckbox("Show_Jaccard_Plot", showJaccardPlot);
gd.addCheckbox("Use_max_residuals", useMaxResiduals);
gd.addCheckbox("Logging", analysisLogging);
gd.addStringField("Title", analysisTitle);
gd.addCheckbox("Save_template", saveTemplate);
// Add a mouse listener to the config file field
if (Utils.isShowGenericDialog()) {
Vector<TextField> numerics = (Vector<TextField>) gd.getNumericFields();
Vector<Checkbox> checkboxes = (Vector<Checkbox>) gd.getCheckboxes();
Vector<Choice> choices = (Vector<Choice>) gd.getChoices();
int n = 0;
choices.get(1).addItemListener(this);
checkboxes.get(0).addItemListener(this);
cbSmartFilter = checkboxes.get(1);
textCoordinateShiftFactor = numerics.get(n++);
textSignalStrength = numerics.get(n++);
textMinPhotons = numerics.get(n++);
textMinWidthFactor = numerics.get(n++);
textWidthFactor = numerics.get(n++);
textPrecisionThreshold = numerics.get(n++);
cbLocalBackground = checkboxes.get(2);
}
gd.showDialog();
if (gd.wasCanceled())
return false;
if (gd.invalidNumber())
return false;
selectionCriteria = gd.getNextChoiceIndex();
// Ignore the template
gd.getNextChoice();
analysisUseBenchmarkSettings = gd.getNextBoolean();
fitConfig.setSmartFilter(gd.getNextBoolean());
filterFitConfig.setCoordinateShiftFactor(gd.getNextNumber());
filterFitConfig.setSignalStrength(gd.getNextNumber());
filterFitConfig.setMinPhotons(gd.getNextNumber());
filterFitConfig.setMinWidthFactor(gd.getNextNumber());
filterFitConfig.setWidthFactor(gd.getNextNumber());
filterFitConfig.setPrecisionThreshold(gd.getNextNumber());
filterFitConfig.setPrecisionUsingBackground(gd.getNextBoolean());
analysisDriftAngle = gd.getNextNumber();
minGap = gd.getNextNumber();
analysisShowResults = gd.getNextBoolean();
showJaccardPlot = gd.getNextBoolean();
useMaxResiduals = gd.getNextBoolean();
analysisLogging = gd.getNextBoolean();
analysisTitle = gd.getNextString();
saveTemplate = gd.getNextBoolean();
if (gd.invalidNumber())
return false;
if (analysisUseBenchmarkSettings) {
if (!updateFilterConfiguration(filterFitConfig))
return false;
}
return true;
}
use of ij.gui.GenericDialog in project GDSC-SMLM by aherbert.
the class DoubletAnalysis method showDialog.
/**
* Show dialog.
*
* @return true, if successful
*/
@SuppressWarnings("unchecked")
private boolean showDialog() {
GenericDialog gd = new GenericDialog(TITLE);
gd.addHelp(About.HELP_URL);
final double sa = getSa();
gd.addMessage(String.format("Fits the benchmark image created by CreateData plugin.\nPSF width = %s, adjusted = %s", Utils.rounded(simulationParameters.s / simulationParameters.a), Utils.rounded(sa)));
// For each new benchmark width, reset the PSF width to the square pixel adjustment
if (lastId != simulationParameters.id) {
double w = sa;
matchDistance = w * Gaussian2DFunction.SD_TO_HWHM_FACTOR;
lowerDistance = 0.5 * matchDistance;
fitConfig.setInitialPeakStdDev(w);
cal.setNmPerPixel(simulationParameters.a);
cal.setGain(simulationParameters.gain);
cal.setAmplification(simulationParameters.amplification);
cal.setExposureTime(100);
cal.setReadNoise(simulationParameters.readNoise);
cal.setBias(simulationParameters.bias);
cal.setEmCCD(simulationParameters.emCCD);
fitConfig.setGain(cal.getGain());
fitConfig.setBias(cal.getBias());
fitConfig.setReadNoise(cal.getReadNoise());
fitConfig.setAmplification(cal.getAmplification());
}
// Support for using templates
String[] templates = ConfigurationTemplate.getTemplateNames(true);
gd.addChoice("Template", templates, templates[0]);
// Allow the settings from the benchmark analysis to be used
gd.addCheckbox("Benchmark_settings", useBenchmarkSettings);
// Collect options for fitting
gd.addNumericField("Initial_StdDev", fitConfig.getInitialPeakStdDev0(), 3);
String[] filterTypes = SettingsManager.getNames((Object[]) DataFilterType.values());
gd.addChoice("Spot_filter_type", filterTypes, filterTypes[config.getDataFilterType().ordinal()]);
String[] filterNames = SettingsManager.getNames((Object[]) DataFilter.values());
gd.addChoice("Spot_filter", filterNames, filterNames[config.getDataFilter(0).ordinal()]);
gd.addSlider("Smoothing", 0, 2.5, config.getSmooth(0));
gd.addSlider("Search_width", 0.5, 2.5, config.getSearch());
gd.addSlider("Border", 0.5, 2.5, config.getBorder());
gd.addSlider("Fitting_width", 2, 4.5, config.getFitting());
String[] solverNames = SettingsManager.getNames((Object[]) FitSolver.values());
gd.addChoice("Fit_solver", solverNames, solverNames[fitConfig.getFitSolver().ordinal()]);
String[] functionNames = SettingsManager.getNames((Object[]) FitFunction.values());
gd.addChoice("Fit_function", functionNames, functionNames[fitConfig.getFitFunction().ordinal()]);
gd.addSlider("Iteration_increase", 1, 4.5, iterationIncrease);
gd.addCheckbox("Ignore_with_neighbours", ignoreWithNeighbours);
gd.addCheckbox("Show_overlay", showOverlay);
gd.addCheckbox("Show_histograms", showHistograms);
gd.addCheckbox("Show_results", showResults);
gd.addCheckbox("Show_Jaccard_Plot", showJaccardPlot);
gd.addCheckbox("Use_max_residuals", useMaxResiduals);
gd.addNumericField("Match_distance", matchDistance, 2);
gd.addNumericField("Lower_distance", lowerDistance, 2);
gd.addNumericField("Signal_factor", signalFactor, 2);
gd.addNumericField("Lower_factor", lowerSignalFactor, 2);
gd.addChoice("Matching", MATCHING, MATCHING[matching]);
// Add a mouse listener to the config file field
if (Utils.isShowGenericDialog()) {
Vector<TextField> numerics = (Vector<TextField>) gd.getNumericFields();
Vector<Choice> choices = (Vector<Choice>) gd.getChoices();
int n = 0;
int ch = 0;
choices.get(ch++).addItemListener(this);
Checkbox b = (Checkbox) gd.getCheckboxes().get(0);
b.addItemListener(this);
textInitialPeakStdDev0 = numerics.get(n++);
textDataFilterType = choices.get(ch++);
textDataFilter = choices.get(ch++);
textSmooth = numerics.get(n++);
textSearch = numerics.get(n++);
textBorder = numerics.get(n++);
textFitting = numerics.get(n++);
textFitSolver = choices.get(ch++);
textFitFunction = choices.get(ch++);
// Iteration increase
n++;
textMatchDistance = numerics.get(n++);
textLowerDistance = numerics.get(n++);
textSignalFactor = numerics.get(n++);
textLowerFactor = numerics.get(n++);
}
gd.showDialog();
if (gd.wasCanceled())
return false;
// Ignore the template
gd.getNextChoice();
useBenchmarkSettings = gd.getNextBoolean();
fitConfig.setInitialPeakStdDev(gd.getNextNumber());
config.setDataFilterType(gd.getNextChoiceIndex());
config.setDataFilter(gd.getNextChoiceIndex(), Math.abs(gd.getNextNumber()), 0);
config.setSearch(gd.getNextNumber());
config.setBorder(gd.getNextNumber());
config.setFitting(gd.getNextNumber());
fitConfig.setFitSolver(gd.getNextChoiceIndex());
fitConfig.setFitFunction(gd.getNextChoiceIndex());
// Avoid stupidness. Note: We are mostly ignoring the validation result and
// checking the results for the doublets manually.
// Realistically we cannot fit lower than this
fitConfig.setMinPhotons(15);
// Set the width factors to help establish bounds for bounded fitters
fitConfig.setMinWidthFactor(1.0 / 10);
fitConfig.setWidthFactor(10);
iterationIncrease = gd.getNextNumber();
ignoreWithNeighbours = gd.getNextBoolean();
showOverlay = gd.getNextBoolean();
showHistograms = gd.getNextBoolean();
showResults = gd.getNextBoolean();
showJaccardPlot = gd.getNextBoolean();
useMaxResiduals = gd.getNextBoolean();
matchDistance = Math.abs(gd.getNextNumber());
lowerDistance = Math.abs(gd.getNextNumber());
signalFactor = Math.abs(gd.getNextNumber());
lowerSignalFactor = Math.abs(gd.getNextNumber());
matching = gd.getNextChoiceIndex();
if (gd.invalidNumber())
return false;
if (lowerDistance > matchDistance)
lowerDistance = matchDistance;
if (lowerSignalFactor > signalFactor)
lowerSignalFactor = signalFactor;
if (useBenchmarkSettings) {
if (!updateFitConfiguration(config))
return false;
}
GlobalSettings settings = new GlobalSettings();
settings.setFitEngineConfiguration(config);
settings.setCalibration(cal);
boolean configure = true;
if (useBenchmarkSettings) {
// Only configure the fit solver if not in a macro
configure = Macro.getOptions() == null;
}
if (configure && !PeakFit.configureFitSolver(settings, null, false))
return false;
lastId = simulationParameters.id;
if (showHistograms) {
gd = new GenericDialog(TITLE);
gd.addMessage("Select the histograms to display");
for (int i = 0; i < NAMES.length; i++) gd.addCheckbox(NAMES[i].replace(' ', '_'), displayHistograms[i]);
for (int i = 0; i < NAMES2.length; i++) gd.addCheckbox(NAMES2[i].replace(' ', '_'), displayHistograms[i + NAMES.length]);
gd.showDialog();
if (gd.wasCanceled())
return false;
for (int i = 0; i < displayHistograms.length; i++) displayHistograms[i] = gd.getNextBoolean();
}
return true;
}
use of ij.gui.GenericDialog in project GDSC-SMLM by aherbert.
the class DiffusionRateTest method showDialog.
private boolean showDialog() {
GenericDialog gd = new GenericDialog(TITLE);
GlobalSettings globalSettings = SettingsManager.loadSettings();
settings = globalSettings.getCreateDataSettings();
if (settings.stepsPerSecond < 1)
settings.stepsPerSecond = 1;
gd.addNumericField("Pixel_pitch (nm)", settings.pixelPitch, 2);
gd.addNumericField("Seconds", settings.seconds, 1);
gd.addSlider("Steps_per_second", 1, 15, settings.stepsPerSecond);
if (extraOptions) {
gd.addSlider("Aggregate_steps", 2, 20, aggregateSteps);
gd.addNumericField("MSD_analysis_steps", msdAnalysisSteps, 0);
}
gd.addNumericField("Particles", settings.particles, 0);
gd.addNumericField("Diffusion_rate (um^2/sec)", settings.diffusionRate, 2);
if (extraOptions)
gd.addNumericField("Precision (nm)", precision, 2);
String[] diffusionTypes = SettingsManager.getNames((Object[]) DiffusionType.values());
gd.addChoice("Diffusion_type", diffusionTypes, diffusionTypes[settings.getDiffusionType().ordinal()]);
gd.addCheckbox("Use_confinement", useConfinement);
gd.addSlider("Confinement_attempts", 1, 20, confinementAttempts);
gd.addSlider("Confinement_radius (nm)", 0, 3000, settings.confinementRadius);
gd.addSlider("Fit_N", 5, 20, fitN);
gd.addCheckbox("Show_example", showDiffusionExample);
gd.addSlider("Magnification", 1, 10, magnification);
gd.showDialog();
if (gd.wasCanceled())
return false;
settings.pixelPitch = Math.abs(gd.getNextNumber());
settings.seconds = Math.abs(gd.getNextNumber());
settings.stepsPerSecond = Math.abs(gd.getNextNumber());
if (extraOptions) {
myAggregateSteps = aggregateSteps = Math.abs((int) gd.getNextNumber());
myMsdAnalysisSteps = msdAnalysisSteps = Math.abs((int) gd.getNextNumber());
}
settings.particles = Math.abs((int) gd.getNextNumber());
settings.diffusionRate = Math.abs(gd.getNextNumber());
if (extraOptions)
myPrecision = precision = Math.abs(gd.getNextNumber());
settings.setDiffusionType(gd.getNextChoiceIndex());
useConfinement = gd.getNextBoolean();
confinementAttempts = Math.abs((int) gd.getNextNumber());
settings.confinementRadius = Math.abs(gd.getNextNumber());
fitN = Math.abs((int) gd.getNextNumber());
showDiffusionExample = gd.getNextBoolean();
magnification = gd.getNextNumber();
// Save before validation so that the current values are preserved.
SettingsManager.saveSettings(globalSettings);
// Check arguments
try {
Parameters.isAboveZero("Pixel Pitch", settings.pixelPitch);
Parameters.isAboveZero("Seconds", settings.seconds);
Parameters.isAboveZero("Steps per second", settings.stepsPerSecond);
Parameters.isAboveZero("Particles", settings.particles);
Parameters.isPositive("Diffusion rate", settings.diffusionRate);
Parameters.isAboveZero("Magnification", magnification);
Parameters.isAboveZero("Confinement attempts", confinementAttempts);
Parameters.isAboveZero("Fit N", fitN);
} catch (IllegalArgumentException e) {
IJ.error(TITLE, e.getMessage());
return false;
}
if (settings.diffusionRate == 0)
IJ.error(TITLE, "Warning : Diffusion rate is zero");
if (gd.invalidNumber())
return false;
SettingsManager.saveSettings(globalSettings);
return true;
}
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