use of loci.common.services.DependencyException in project bioformats by openmicroscopy.
the class CellWorxReader method initFile.
// -- Internal FormatReader API methods --
/* @see loci.formats.FormatReader#initFile(String) */
@Override
protected void initFile(String id) throws FormatException, IOException {
if (!checkSuffix(id, "htd")) {
LOGGER.info("Searching for .htd file");
String base = new Location(id).getAbsolutePath();
base = base.substring(0, base.lastIndexOf("_"));
id = base + ".HTD";
if (!new Location(id).exists()) {
Location parent = new Location(id).getAbsoluteFile().getParentFile();
directoryList = parent.list(true);
for (String f : directoryList) {
if (checkSuffix(f, "htd")) {
id = new Location(parent, f).getAbsolutePath();
LOGGER.info("Found .htd file {}", f);
break;
}
}
}
}
super.initFile(id);
try {
ServiceFactory factory = new ServiceFactory();
service = factory.getInstance(OMEXMLService.class);
} catch (DependencyException exc) {
throw new FormatException("Could not create OME-XML store.", exc);
}
String plateData = DataTools.readFile(id);
String[] lines = plateData.split("\n");
int xWells = 0, yWells = 0;
int xFields = 0, yFields = 0;
String[] wavelengths = null;
int nTimepoints = 1;
// determine dataset dimensions
for (String line : lines) {
int split = line.indexOf("\",");
if (split < 1)
continue;
String key = line.substring(1, split).trim();
String value = line.substring(split + 2).trim();
if (key.equals("XWells")) {
xWells = Integer.parseInt(value);
} else if (key.equals("YWells")) {
yWells = Integer.parseInt(value);
wellFiles = new String[yWells][xWells][];
logFiles = new String[yWells][xWells];
} else if (key.startsWith("WellsSelection")) {
int row = Integer.parseInt(key.substring(14)) - 1;
String[] mapping = value.split(",");
for (int col = 0; col < xWells; col++) {
if (new Boolean(mapping[col].trim()).booleanValue()) {
wellFiles[row][col] = new String[1];
}
}
} else if (key.equals("XSites")) {
xFields = Integer.parseInt(value);
} else if (key.equals("YSites")) {
yFields = Integer.parseInt(value);
fieldMap = new boolean[yFields][xFields];
} else if (key.equals("TimePoints")) {
nTimepoints = Integer.parseInt(value);
} else if (key.startsWith("SiteSelection")) {
int row = Integer.parseInt(key.substring(13)) - 1;
String[] mapping = value.split(",");
for (int col = 0; col < xFields; col++) {
fieldMap[row][col] = new Boolean(mapping[col].trim()).booleanValue();
}
} else if (key.equals("Waves")) {
doChannels = new Boolean(value.toLowerCase());
} else if (key.equals("NWavelengths")) {
wavelengths = new String[Integer.parseInt(value)];
} else if (key.startsWith("WaveName")) {
int index = Integer.parseInt(key.substring(8)) - 1;
wavelengths[index] = value.replaceAll("\"", "");
}
}
for (int row = 0; row < fieldMap.length; row++) {
for (int col = 0; col < fieldMap[row].length; col++) {
if (fieldMap[row][col])
fieldCount++;
}
}
// find pixels files
String plateName = new Location(id).getAbsolutePath();
plateName = plateName.substring(0, plateName.lastIndexOf(".")) + "_";
int wellCount = 0;
for (int row = 0; row < wellFiles.length; row++) {
for (int col = 0; col < wellFiles[row].length; col++) {
if (wellFiles[row][col] != null) {
wellCount++;
char rowLetter = (char) (row + 'A');
String base = plateName + rowLetter + String.format("%02d", col + 1);
wellFiles[row][col][0] = base + ".pnl";
logFiles[row][col] = base + "_scan.log";
if (!new Location(wellFiles[row][col][0]).exists()) {
// using TIFF files instead
wellFiles[row][col] = getTiffFiles(plateName, rowLetter, col, wavelengths.length, nTimepoints);
}
}
}
}
plateLogFile = plateName + "scan.log";
String serialNumber = null;
if (new Location(plateLogFile).exists()) {
String[] f = DataTools.readFile(plateLogFile).split("\n");
for (String line : f) {
if (line.trim().startsWith("Z Map File")) {
String file = line.substring(line.indexOf(':') + 1);
file = file.substring(file.lastIndexOf("/") + 1).trim();
String parent = new Location(id).getAbsoluteFile().getParent();
zMapFile = new Location(parent, file).getAbsolutePath();
} else if (line.trim().startsWith("Scanner SN")) {
serialNumber = line.substring(line.indexOf(':') + 1).trim();
}
}
}
int seriesCount = fieldCount * wellCount;
int planeIndex = 0;
int seriesIndex = 0;
String file = getFile(seriesIndex, planeIndex);
while (!new Location(file).exists()) {
if (planeIndex < nTimepoints * wavelengths.length) {
planeIndex++;
} else if (seriesIndex < seriesCount - 1) {
planeIndex = 0;
seriesIndex++;
} else {
break;
}
file = getFile(seriesIndex, planeIndex);
}
IFormatReader pnl = getReader(file, true);
core.clear();
for (int i = 0; i < seriesCount; i++) {
CoreMetadata ms = new CoreMetadata();
core.add(ms);
setSeries(i);
ms.littleEndian = pnl.isLittleEndian();
ms.sizeX = pnl.getSizeX();
ms.sizeY = pnl.getSizeY();
ms.pixelType = pnl.getPixelType();
ms.sizeZ = 1;
ms.sizeT = nTimepoints;
ms.sizeC = wavelengths.length;
ms.imageCount = getSizeZ() * getSizeC() * getSizeT();
ms.dimensionOrder = "XYCZT";
ms.rgb = false;
ms.interleaved = pnl.isInterleaved();
}
OMEXMLMetadata readerMetadata = (OMEXMLMetadata) pnl.getMetadataStore();
OMEXMLMetadataRoot root = (OMEXMLMetadataRoot) readerMetadata.getRoot();
Instrument instrument = root.getInstrument(0);
List<Image> images = root.copyImageList();
OMEXMLMetadataRoot convertRoot = new OMEXMLMetadataRoot();
convertRoot.addInstrument(instrument);
for (int i = 0; i < core.size() / images.size(); i++) {
for (Image img : images) {
convertRoot.addImage(img);
}
}
OMEXMLMetadata convertMetadata;
try {
convertMetadata = service.createOMEXMLMetadata();
} catch (ServiceException exc) {
throw new FormatException("Could not create OME-XML store.", exc);
}
convertMetadata.setRoot(convertRoot);
pnl.close();
MetadataStore store = makeFilterMetadata();
MetadataConverter.convertMetadata(convertMetadata, store);
MetadataTools.populatePixels(store, this);
String plateID = MetadataTools.createLSID("Plate", 0);
Location plate = new Location(id).getAbsoluteFile();
store.setPlateID(plateID, 0);
plateName = plate.getName();
if (plateName.indexOf('.') > 0) {
plateName = plateName.substring(0, plateName.lastIndexOf('.'));
}
store.setPlateName(plateName, 0);
store.setPlateRows(new PositiveInteger(wellFiles.length), 0);
store.setPlateColumns(new PositiveInteger(wellFiles[0].length), 0);
for (int i = 0; i < core.size(); i++) {
store.setImageID(MetadataTools.createLSID("Image", i), i);
}
String plateAcqID = MetadataTools.createLSID("PlateAcquisition", 0, 0);
store.setPlateAcquisitionID(plateAcqID, 0, 0);
PositiveInteger fieldCount = FormatTools.getMaxFieldCount(fieldMap.length * fieldMap[0].length);
if (fieldCount != null) {
store.setPlateAcquisitionMaximumFieldCount(fieldCount, 0, 0);
}
int nextImage = 0;
for (int row = 0; row < wellFiles.length; row++) {
for (int col = 0; col < wellFiles[row].length; col++) {
int wellIndex = row * wellFiles[row].length + col;
String wellID = MetadataTools.createLSID("Well", 0, wellIndex);
store.setWellID(wellID, 0, wellIndex);
store.setWellColumn(new NonNegativeInteger(col), 0, wellIndex);
store.setWellRow(new NonNegativeInteger(row), 0, wellIndex);
int fieldIndex = 0;
for (int fieldRow = 0; fieldRow < fieldMap.length; fieldRow++) {
for (int fieldCol = 0; fieldCol < fieldMap[fieldRow].length; fieldCol++) {
if (fieldMap[fieldRow][fieldCol] && wellFiles[row][col] != null) {
String wellSampleID = MetadataTools.createLSID("WellSample", 0, wellIndex, fieldIndex);
store.setWellSampleID(wellSampleID, 0, wellIndex, fieldIndex);
String imageID = MetadataTools.createLSID("Image", nextImage);
store.setWellSampleImageRef(imageID, 0, wellIndex, fieldIndex);
store.setWellSampleIndex(new NonNegativeInteger(nextImage), 0, wellIndex, fieldIndex);
store.setPlateAcquisitionWellSampleRef(wellSampleID, 0, 0, nextImage);
String well = (char) (row + 'A') + String.format("%02d", col + 1);
store.setImageName("Well " + well + " Field #" + (fieldIndex + 1), nextImage);
nextImage++;
fieldIndex++;
}
}
}
}
}
if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) {
if (serialNumber != null) {
store.setMicroscopeSerialNumber(serialNumber, 0);
}
for (int well = 0; well < wellCount; well++) {
parseWellLogFile(well, store);
}
if (timestamps.size() > 0) {
store.setPlateAcquisitionStartTime(timestamps.get(0), 0, 0);
store.setPlateAcquisitionEndTime(timestamps.get(timestamps.size() - 1), 0, 0);
}
for (int i = 0; i < core.size(); i++) {
for (int c = 0; c < getSizeC(); c++) {
if (c < wavelengths.length && wavelengths[c] != null) {
store.setChannelName(wavelengths[c], i, c);
}
}
}
}
}
use of loci.common.services.DependencyException in project bioformats by openmicroscopy.
the class PlugInBioFormatsImporter method readImage.
public void readImage() {
final ViewUserInterface mipav = ViewUserInterface.getReference();
// prompt user to choose a file
if (chooser == null) {
chooser = GUITools.buildFileChooser(reader);
chooser.setCurrentDirectory(new File(Preferences.getImageDirectory()));
}
JFrame parent = mipav.getMainFrame();
int rval = chooser.showOpenDialog(parent);
// user canceled
if (rval != JFileChooser.APPROVE_OPTION)
return;
final File file = chooser.getSelectedFile();
// load the image in a separate thread
Thread importerThread = new Thread("BioFormats-Importer") {
public void run() {
String name = file.getName();
String dir = file.getParent();
// open file using Bio-Formats
setMessage(mipav, "Importing " + name + "...", true);
String id = file.getPath();
try {
long tic = System.currentTimeMillis();
IMetadata store;
try {
ServiceFactory factory = new ServiceFactory();
OMEXMLService service = factory.getInstance(OMEXMLService.class);
store = service.createOMEXMLMetadata();
} catch (DependencyException exc) {
throw new FormatException("Could not create OME-XML store.", exc);
} catch (ServiceException exc) {
throw new FormatException("Could not create OME-XML store.", exc);
}
reader.setMetadataStore(store);
reader.setId(id);
// MIPAV assumes 4-D data in XYZT order
reader.setOutputOrder("XYZTC");
// harvest some core metadata
int imageCount = reader.getImageCount();
boolean little = reader.isLittleEndian();
int pixelType = reader.getPixelType();
int bpp = FormatTools.getBytesPerPixel(pixelType);
boolean floating = FormatTools.isFloatingPoint(pixelType);
int sizeX = reader.getSizeX();
int sizeY = reader.getSizeY();
int sizeZ = reader.getSizeZ();
int sizeT = reader.getSizeT();
int sizeC = reader.getSizeC();
String imageName = store.getImageName(0);
if (sizeC > 1) {
throw new FormatException("Multichannel data is unsupported at the moment");
}
// compute MIPAV buffer type
int mipavType;
switch(pixelType) {
case FormatTools.INT8:
mipavType = ModelStorageBase.BYTE;
break;
case FormatTools.UINT8:
mipavType = ModelStorageBase.UBYTE;
break;
case FormatTools.INT16:
mipavType = ModelStorageBase.SHORT;
break;
case FormatTools.UINT16:
mipavType = ModelStorageBase.USHORT;
break;
case FormatTools.INT32:
mipavType = ModelStorageBase.INTEGER;
break;
case FormatTools.UINT32:
mipavType = ModelStorageBase.UINTEGER;
break;
case FormatTools.FLOAT:
mipavType = ModelStorageBase.FLOAT;
break;
case FormatTools.DOUBLE:
mipavType = ModelStorageBase.DOUBLE;
break;
default:
throw new FormatException("Unsupported pixel type: " + pixelType);
}
// harvest physical resolution
Length dimPhysSizeX = store.getPixelsPhysicalSizeX(0);
Length dimPhysSizeY = store.getPixelsPhysicalSizeY(0);
Length dimPhysSizeZ = store.getPixelsPhysicalSizeZ(0);
Time dimTimeInc = store.getPixelsTimeIncrement(0);
float physSizeX = dimPhysSizeX == null ? 1.0f : dimPhysSizeX.value(UNITS.MICROMETER).floatValue();
float physSizeY = dimPhysSizeY == null ? 1.0f : dimPhysSizeY.value(UNITS.MICROMETER).floatValue();
float physSizeZ = dimPhysSizeZ == null ? 1.0f : dimPhysSizeZ.value(UNITS.MICROMETER).floatValue();
float timeInc = dimTimeInc == null ? 1.0f : dimTimeInc.value(UNITS.SECOND).floatValue();
// compute dimensional extents
int[] dimExtents = { sizeX, sizeY, sizeZ, sizeT };
float[] res = { physSizeX, physSizeY, physSizeZ, timeInc };
int[] units = { FileInfoBase.MICROMETERS, FileInfoBase.MICROMETERS, FileInfoBase.MICROMETERS, FileInfoBase.SECONDS };
// create MIPAV image object
ModelImage modelImage = new ModelImage(mipavType, dimExtents, imageName);
// import planes into MIPAV image
byte[] buf = new byte[bpp * sizeX * sizeY];
for (int i = 0; i < imageCount; i++) {
setMessage(mipav, "Reading plane #" + (i + 1) + "/" + imageCount, false);
reader.openBytes(i, buf);
// convert byte array to appropriate primitive type
int offset = i * buf.length;
Object array = DataTools.makeDataArray(buf, bpp, floating, little);
// assign data to MIPAV image object
switch(mipavType) {
case ModelStorageBase.BYTE:
case ModelStorageBase.UBYTE:
modelImage.importData(offset, (byte[]) array, false);
break;
case ModelStorageBase.SHORT:
case ModelStorageBase.USHORT:
modelImage.importData(offset, (short[]) array, false);
break;
case ModelStorageBase.INTEGER:
case ModelStorageBase.UINTEGER:
modelImage.importData(offset, (int[]) array, false);
break;
case ModelStorageBase.FLOAT:
modelImage.importData(offset, (float[]) array, false);
break;
case ModelStorageBase.DOUBLE:
modelImage.importData(offset, (double[]) array, false);
break;
default:
throw new FormatException("Unknown buffer type: " + mipavType);
}
}
setMessage(mipav, "Finishing import...", true);
// create a FileInfo object for each image plane
FileInfoBase[] fileInfo = new FileInfoBase[imageCount];
for (int i = 0; i < imageCount; i++) {
// HACK: Use FileInfoImageXML since FileInfoBase is abstract.
fileInfo[i] = new FileInfoImageXML(name, dir, FileUtility.XML);
fileInfo[i].setExtents(dimExtents);
fileInfo[i].setResolutions(res);
fileInfo[i].setUnitsOfMeasure(units);
fileInfo[i].setDataType(mipavType);
}
modelImage.setFileInfo(fileInfo);
// scale color range and display MIPAV image
modelImage.calcMinMax();
new ViewJFrameImage(modelImage);
long toc = System.currentTimeMillis();
long time = toc - tic;
long avg = time / imageCount;
setMessage(mipav, name + ": Read " + imageCount + " planes in " + (time / 1000f) + " seconds (" + avg + " ms/plane)", true);
} catch (FormatException exc) {
exc.printStackTrace();
MipavUtil.displayError("An error occurred parsing the file: " + exc.getMessage());
} catch (IOException exc) {
exc.printStackTrace();
MipavUtil.displayError("An I/O error occurred reading the file: " + exc.getMessage());
}
}
};
importerThread.start();
}
use of loci.common.services.DependencyException in project bioformats by openmicroscopy.
the class SixteenBitLosslessJPEG2000Test method testLosslessPixels.
@Test
public void testLosslessPixels() throws Exception {
int failureCount = 0;
for (int v = Short.MIN_VALUE; v < Short.MAX_VALUE; v += increment) {
int index = v + Short.MAX_VALUE + 1;
byte[] pixels = DataTools.shortToBytes((short) v, false);
String file = index + ".jp2";
ByteArrayHandle tmpFile = new ByteArrayHandle(1);
Location.mapFile(file, tmpFile);
IMetadata metadata16;
try {
ServiceFactory factory = new ServiceFactory();
OMEXMLService service = factory.getInstance(OMEXMLService.class);
metadata16 = service.createOMEXMLMetadata();
} catch (DependencyException exc) {
throw new FormatException("Could not create OME-XML store.", exc);
} catch (ServiceException exc) {
throw new FormatException("Could not create OME-XML store.", exc);
}
MetadataTools.populateMetadata(metadata16, 0, "foo", false, "XYCZT", "uint16", 1, 1, 1, 1, 1, 1);
IFormatWriter writer16 = new JPEG2000Writer();
writer16.setMetadataRetrieve(metadata16);
writer16.setId(file);
writer16.saveBytes(0, pixels);
writer16.close();
byte[] buf = tmpFile.getBytes();
byte[] realData = new byte[(int) tmpFile.length()];
System.arraycopy(buf, 0, realData, 0, realData.length);
tmpFile.close();
tmpFile = new ByteArrayHandle(realData);
Location.mapFile(file, tmpFile);
ImageReader reader = new ImageReader();
reader.setId(file);
byte[] plane = reader.openBytes(0);
for (int q = 0; q < plane.length; q++) {
if (plane[q] != pixels[q]) {
LOGGER.debug("FAILED on {}", DataTools.bytesToShort(pixels, false));
failureCount++;
break;
}
}
reader.close();
tmpFile.close();
Location.mapFile(file, null);
}
assertEquals(failureCount, 0);
}
use of loci.common.services.DependencyException in project bioformats by openmicroscopy.
the class JPEGXRCodec method initialize.
// -- Helper methods --
/**
* Initializes the JPEG-XR dependency service. This is called at the
* beginning of the {@link #decompress} method to avoid having the
* constructor's method definition contain a checked exception.
*
* @throws FormatException If there is an error initializing JPEG-XR
* services.
*/
private void initialize() throws FormatException {
if (service != null)
return;
try {
ServiceFactory factory = new ServiceFactory();
service = factory.getInstance(JPEGXRService.class);
} catch (DependencyException e) {
throw new MissingLibraryException("JPEG-XR library not available", e);
}
}
use of loci.common.services.DependencyException in project bioformats by openmicroscopy.
the class APLReader method initFile.
// -- Internal FormatReader API methods --
/* @see loci.formats.FormatReader#initFile(String) */
@Override
protected void initFile(String id) throws FormatException, IOException {
super.initFile(id);
LOGGER.debug("Initializing {}", id);
// find the corresponding .mtb file
if (!checkSuffix(id, "mtb")) {
if (checkSuffix(id, METADATA_SUFFIXES)) {
int separator = id.lastIndexOf(File.separator);
if (separator < 0)
separator = 0;
int underscore = id.lastIndexOf("_");
if (underscore < separator || checkSuffix(id, "apl")) {
underscore = id.lastIndexOf(".");
}
String mtbFile = id.substring(0, underscore) + "_d.mtb";
if (!new Location(mtbFile).exists()) {
throw new FormatException(".mtb file not found");
}
currentId = new Location(mtbFile).getAbsolutePath();
} else {
Location parent = new Location(id).getAbsoluteFile().getParentFile();
parent = parent.getParentFile();
String[] list = parent.list(true);
for (String f : list) {
if (checkSuffix(f, "mtb")) {
currentId = new Location(parent, f).getAbsolutePath();
break;
}
}
if (!checkSuffix(currentId, "mtb")) {
throw new FormatException(".mtb file not found");
}
}
}
String mtb = new Location(currentId).getAbsolutePath();
LOGGER.debug("Reading .mtb file '{}'", mtb);
MDBService mdb = null;
try {
ServiceFactory factory = new ServiceFactory();
mdb = factory.getInstance(MDBService.class);
} catch (DependencyException de) {
throw new FormatException("MDB Tools Java library not found", de);
}
String[] columnNames = null;
List<String[]> rows = null;
try {
mdb.initialize(mtb);
rows = mdb.parseDatabase().get(0);
columnNames = rows.get(0);
String[] tmpNames = columnNames;
columnNames = new String[tmpNames.length - 1];
System.arraycopy(tmpNames, 1, columnNames, 0, columnNames.length);
} finally {
mdb.close();
}
if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) {
for (int i = 1; i < rows.size(); i++) {
String[] row = rows.get(i);
for (int q = 0; q < row.length; q++) {
addGlobalMetaList(columnNames[q], row[q]);
}
}
}
used = new ArrayList<String>();
used.add(mtb);
String tnb = mtb.substring(0, mtb.lastIndexOf("."));
if (tnb.lastIndexOf("_") > tnb.lastIndexOf(File.separator)) {
tnb = tnb.substring(0, tnb.lastIndexOf("_"));
}
used.add(tnb + "_1.tnb");
used.add(tnb + ".apl");
String idPath = new Location(id).getAbsolutePath();
if (!used.contains(idPath) && checkSuffix(idPath, METADATA_SUFFIXES)) {
used.add(idPath);
}
// calculate indexes to relevant metadata
int calibrationUnit = DataTools.indexOf(columnNames, "Calibration Unit");
int colorChannels = DataTools.indexOf(columnNames, "Color Channels");
int frames = DataTools.indexOf(columnNames, "Frames");
int calibratedHeight = DataTools.indexOf(columnNames, "Height");
int calibratedWidth = DataTools.indexOf(columnNames, "Width");
int path = DataTools.indexOf(columnNames, "Image Path");
if (path == -1) {
path = DataTools.indexOf(columnNames, "Path");
}
int filename = DataTools.indexOf(columnNames, "File Name");
int magnification = DataTools.indexOf(columnNames, "Magnification");
int width = DataTools.indexOf(columnNames, "X-Resolution");
int height = DataTools.indexOf(columnNames, "Y-Resolution");
int imageType = DataTools.indexOf(columnNames, "Image Type");
int imageName = DataTools.indexOf(columnNames, "Image Name");
int zLayers = DataTools.indexOf(columnNames, "Z-Layers");
String parentDirectory = mtb.substring(0, mtb.lastIndexOf(File.separator));
// look for the directory that contains TIFF and XML files
LOGGER.debug("Searching {} for a directory with TIFFs", parentDirectory);
Location dir = new Location(parentDirectory);
String topDirectory = null;
int index = 2;
String pathName = rows.get(index++)[path].trim();
while (pathName.equals("") && index < rows.size()) {
pathName = rows.get(index++)[path].trim();
}
pathName = pathName.replace('\\', File.separatorChar);
pathName = pathName.replaceAll("/", File.separator);
String[] dirs = pathName.split(File.separatorChar == '\\' ? "\\\\" : File.separator);
for (int i = dirs.length - 1; i >= 0; i--) {
if (dirs[i].indexOf("_DocumentFiles") > 0) {
Location file = new Location(dir, dirs[i]);
if (file.exists()) {
topDirectory = file.getAbsolutePath();
break;
}
}
}
if (topDirectory == null) {
String[] list = dir.list();
for (String f : list) {
LOGGER.debug(" '{}'", f);
Location file = new Location(dir, f);
if (file.isDirectory() && f.indexOf("_DocumentFiles") > 0) {
topDirectory = file.getAbsolutePath();
LOGGER.debug("Found {}", topDirectory);
break;
}
}
}
if (topDirectory == null) {
throw new FormatException("Could not find a directory with TIFF files.");
}
final List<Integer> seriesIndexes = new ArrayList<Integer>();
for (int i = 1; i < rows.size(); i++) {
String file = parseFilename(rows.get(i), filename, path);
if (file.equals(""))
continue;
file = topDirectory + File.separator + file;
if (new Location(file).exists() && checkSuffix(file, "tif")) {
seriesIndexes.add(i);
}
}
int seriesCount = seriesIndexes.size();
core.clear();
tiffFiles = new String[seriesCount];
xmlFiles = new String[seriesCount];
parser = new TiffParser[seriesCount];
ifds = new IFDList[seriesCount];
for (int i = 0; i < seriesCount; i++) {
CoreMetadata ms = new CoreMetadata();
core.add(ms);
int secondRow = seriesIndexes.get(i);
int firstRow = secondRow - 1;
String[] row2 = rows.get(firstRow);
String[] row3 = rows.get(secondRow);
if (frames > -1) {
ms.sizeT = parseDimension(row3[frames]);
}
if (zLayers > -1) {
ms.sizeZ = parseDimension(row3[zLayers]);
}
if (colorChannels > -1) {
ms.sizeC = parseDimension(row3[colorChannels]);
} else if (imageType > -1) {
if (row3[imageType] != null && row3[imageType].equals("RGB")) {
ms.sizeC = 3;
}
}
ms.dimensionOrder = "XYCZT";
if (ms.sizeZ == 0)
ms.sizeZ = 1;
if (ms.sizeC == 0)
ms.sizeC = 1;
if (ms.sizeT == 0)
ms.sizeT = 1;
xmlFiles[i] = topDirectory + File.separator + parseFilename(row2, filename, path);
tiffFiles[i] = topDirectory + File.separator + parseFilename(row3, filename, path);
parser[i] = new TiffParser(tiffFiles[i]);
parser[i].setDoCaching(false);
ifds[i] = parser[i].getIFDs();
for (IFD ifd : ifds[i]) {
parser[i].fillInIFD(ifd);
}
// get core metadata from TIFF file
IFD ifd = ifds[i].get(0);
PhotoInterp photo = ifd.getPhotometricInterpretation();
int samples = ifd.getSamplesPerPixel();
ms.sizeX = (int) ifd.getImageWidth();
ms.sizeY = (int) ifd.getImageLength();
ms.rgb = samples > 1 || photo == PhotoInterp.RGB;
ms.pixelType = ifd.getPixelType();
ms.littleEndian = ifd.isLittleEndian();
ms.indexed = photo == PhotoInterp.RGB_PALETTE && ifd.containsKey(IFD.COLOR_MAP);
ms.imageCount = ifds[i].size();
if (ms.sizeZ * ms.sizeT * (ms.rgb ? 1 : ms.sizeC) != ms.imageCount) {
ms.sizeT = ms.imageCount / (ms.rgb ? 1 : ms.sizeC);
ms.sizeZ = 1;
}
}
MetadataStore store = makeFilterMetadata();
MetadataTools.populatePixels(store, this);
for (int i = 0; i < seriesCount; i++) {
String[] row = rows.get(seriesIndexes.get(i));
// populate Image data
MetadataTools.setDefaultCreationDate(store, mtb, i);
store.setImageName(row[imageName].trim(), i);
if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) {
// populate Dimensions data
// calculate physical X and Y sizes
double realWidth = Double.parseDouble(row[calibratedWidth]);
double realHeight = Double.parseDouble(row[calibratedHeight]);
String units = row[calibrationUnit];
CoreMetadata ms = core.get(i);
double px = realWidth / ms.sizeX;
double py = realHeight / ms.sizeY;
Length physicalSizeX = FormatTools.getPhysicalSizeX(px, units);
Length physicalSizeY = FormatTools.getPhysicalSizeY(py, units);
if (physicalSizeX != null) {
store.setPixelsPhysicalSizeX(physicalSizeX, i);
}
if (physicalSizeY != null) {
store.setPixelsPhysicalSizeY(physicalSizeY, i);
}
}
}
setSeries(0);
}
Aggregations