Examples with CoreMetadata loci.formats.CoreMetadata used on opensource projects

Example 91 with CoreMetadata

use of loci.formats.CoreMetadata in project bioformats by openmicroscopy.

the class AliconaReader method initFile.

// -- Internal FormatReader API methods --
/* @see loci.formats.FormatReader#initFile(String) */
@Override
protected void initFile(String id) throws FormatException, IOException {
    super.initFile(id);
    in = new RandomAccessInputStream(id);
    CoreMetadata m = core.get(0);
    // check that this is a valid AL3D file
    LOGGER.info("Verifying Alicona format");
    String magicString = in.readString(17);
    if (!magicString.trim().equals("AliconaImaging")) {
        throw new FormatException("Invalid magic string : " + "expected 'AliconaImaging', got " + magicString);
    }
    // now we read a series of tags
    // each one is 52 bytes - 20 byte key + 30 byte value + 2 byte CRLF
    LOGGER.info("Reading tags");
    int count = 2;
    boolean hasC = false;
    String voltage = null, magnification = null, workingDistance = null;
    String pntX = null, pntY = null;
    int depthOffset = 0;
    for (int i = 0; i < count; i++) {
        String key = in.readString(20).trim();
        String value = in.readString(30).trim();
        addGlobalMeta(key, value);
        in.skipBytes(2);
        if (key.equals("TagCount"))
            count += Integer.parseInt(value);
        else if (key.equals("Rows"))
            m.sizeY = Integer.parseInt(value);
        else if (key.equals("Cols"))
            m.sizeX = Integer.parseInt(value);
        else if (key.equals("NumberOfPlanes")) {
            m.imageCount = Integer.parseInt(value);
        } else if (key.equals("TextureImageOffset")) {
            textureOffset = Integer.parseInt(value);
        } else if (key.equals("TexturePtr") && !value.equals("7"))
            hasC = true;
        else if (key.equals("Voltage"))
            voltage = value;
        else if (key.equals("Magnification"))
            magnification = value;
        else if (key.equals("PixelSizeXMeter"))
            pntX = value;
        else if (key.equals("PixelSizeYMeter"))
            pntY = value;
        else if (key.equals("WorkingDistance"))
            workingDistance = value;
        else if (key.equals("DepthImageOffset")) {
            depthOffset = Integer.parseInt(value);
        }
    }
    LOGGER.info("Populating metadata");
    if (textureOffset != 0) {
        numBytes = (int) (in.length() - textureOffset) / (getSizeX() * getSizeY() * getImageCount());
        m.sizeC = hasC ? 3 : 1;
        m.sizeZ = 1;
        m.sizeT = getImageCount() / getSizeC();
        m.pixelType = FormatTools.pixelTypeFromBytes(numBytes, false, false);
    } else {
        textureOffset = depthOffset;
        m.pixelType = FormatTools.FLOAT;
        m.sizeC = 1;
        m.sizeZ = 1;
        m.sizeT = 1;
        m.imageCount = 1;
    }
    m.rgb = false;
    m.interleaved = false;
    m.littleEndian = true;
    m.dimensionOrder = "XYCTZ";
    m.metadataComplete = true;
    m.indexed = false;
    m.falseColor = false;
    MetadataStore store = makeFilterMetadata();
    MetadataTools.populatePixels(store, this);
    if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) {
        // link Image and Instrument
        String instrumentID = MetadataTools.createLSID("Instrument", 0);
        store.setInstrumentID(instrumentID, 0);
        store.setImageInstrumentRef(instrumentID, 0);
        // used when the dataset was acquired, i.e. detector settings.
        if (voltage != null) {
            store.setDetectorSettingsVoltage(new ElectricPotential(new Double(voltage), UNITS.VOLT), 0, 0);
            // link DetectorSettings to an actual Detector
            String detectorID = MetadataTools.createLSID("Detector", 0, 0);
            store.setDetectorID(detectorID, 0, 0);
            store.setDetectorSettingsID(detectorID, 0, 0);
            // set required Detector type
            store.setDetectorType(getDetectorType("Other"), 0, 0);
        }
        if (magnification != null) {
            store.setObjectiveCalibratedMagnification(new Double(magnification), 0, 0);
        }
        if (workingDistance != null) {
            store.setObjectiveWorkingDistance(new Length(new Double(workingDistance), UNITS.MICROMETER), 0, 0);
        }
        store.setObjectiveCorrection(getCorrection("Other"), 0, 0);
        store.setObjectiveImmersion(getImmersion("Other"), 0, 0);
        // link Objective to an Image using ObjectiveSettings
        String objectiveID = MetadataTools.createLSID("Objective", 0, 0);
        store.setObjectiveID(objectiveID, 0, 0);
        store.setObjectiveSettingsID(objectiveID, 0);
        if (pntX != null && pntY != null) {
            double pixelSizeX = Double.parseDouble(pntX);
            double pixelSizeY = Double.parseDouble(pntY);
            Length sizeX = FormatTools.getPhysicalSizeX(pixelSizeX, UNITS.METER);
            Length sizeY = FormatTools.getPhysicalSizeY(pixelSizeY, UNITS.METER);
            if (sizeX != null) {
                store.setPixelsPhysicalSizeX(sizeX, 0);
            }
            if (sizeY != null) {
                store.setPixelsPhysicalSizeY(sizeY, 0);
            }
        }
    }
}

Example 92 with CoreMetadata

use of loci.formats.CoreMetadata in project bioformats by openmicroscopy.

the class AnalyzeReader method initFile.

// -- Internal FormatReader API methods --
/* @see loci.formats.FormatReader#initFile(String) */
@Override
protected void initFile(String id) throws FormatException, IOException {
    // the dataset has two files - we want the one ending in '.hdr'
    if (id.endsWith(".img")) {
        LOGGER.info("Looking for header file");
        String header = id.substring(0, id.lastIndexOf(".")) + ".hdr";
        if (new Location(header).exists()) {
            setId(header);
            return;
        } else
            throw new FormatException("Header file not found.");
    }
    super.initFile(id);
    in = new RandomAccessInputStream(id);
    pixelsFilename = id.substring(0, id.lastIndexOf(".")) + ".img";
    pixelFile = new RandomAccessInputStream(pixelsFilename);
    LOGGER.info("Reading header");
    int fileSize = in.readInt();
    boolean little = fileSize != in.length();
    in.order(little);
    pixelFile.order(little);
    in.skipBytes(10);
    String imageName = in.readString(18);
    in.skipBytes(8);
    int ndims = in.readShort();
    int x = in.readShort();
    int y = in.readShort();
    int z = in.readShort();
    int t = in.readShort();
    in.skipBytes(20);
    int dataType = in.readShort();
    int nBitsPerPixel = in.readShort();
    String description = null;
    double voxelWidth = 0d, voxelHeight = 0d, sliceThickness = 0d, deltaT = 0d;
    if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) {
        in.skipBytes(6);
        voxelWidth = in.readFloat();
        voxelHeight = in.readFloat();
        sliceThickness = in.readFloat();
        deltaT = in.readFloat();
        in.skipBytes(12);
        pixelOffset = (int) in.readFloat();
        in.skipBytes(12);
        float calibratedMax = in.readFloat();
        float calibratedMin = in.readFloat();
        float compressed = in.readFloat();
        float verified = in.readFloat();
        float pixelMax = in.readFloat();
        float pixelMin = in.readFloat();
        description = in.readString(80);
        String auxFile = in.readString(24);
        char orient = (char) in.readByte();
        String originator = in.readString(10);
        String generated = in.readString(10);
        String scannum = in.readString(10);
        String patientID = in.readString(10);
        String expDate = in.readString(10);
        String expTime = in.readString(10);
        in.skipBytes(3);
        int views = in.readInt();
        int volsAdded = in.readInt();
        int startField = in.readInt();
        int fieldSkip = in.readInt();
        int omax = in.readInt();
        int omin = in.readInt();
        int smax = in.readInt();
        int smin = in.readInt();
        addGlobalMeta("Database name", imageName);
        addGlobalMeta("Number of dimensions", ndims);
        addGlobalMeta("Data type", dataType);
        addGlobalMeta("Number of bits per pixel", nBitsPerPixel);
        addGlobalMeta("Voxel width", voxelWidth);
        addGlobalMeta("Voxel height", voxelHeight);
        addGlobalMeta("Slice thickness", sliceThickness);
        addGlobalMeta("Exposure time", deltaT);
        addGlobalMeta("Pixel offset", pixelOffset);
        addGlobalMeta("Calibrated maximum", calibratedMax);
        addGlobalMeta("Calibrated minimum", calibratedMin);
        addGlobalMeta("Compressed", compressed);
        addGlobalMeta("Verified", verified);
        addGlobalMeta("Pixel maximum", pixelMax);
        addGlobalMeta("Pixel minimum", pixelMin);
        addGlobalMeta("Description", description);
        addGlobalMeta("Auxiliary file", auxFile);
        addGlobalMeta("Orientation", orient);
        addGlobalMeta("Originator", originator);
        addGlobalMeta("Generated", generated);
        addGlobalMeta("Scan Number", scannum);
        addGlobalMeta("Patient ID", patientID);
        addGlobalMeta("Acquisition Date", expDate);
        addGlobalMeta("Acquisition Time", expTime);
    } else {
        in.skipBytes(34);
        pixelOffset = (int) in.readFloat();
    }
    LOGGER.info("Populating core metadata");
    CoreMetadata m = core.get(0);
    m.littleEndian = little;
    m.sizeX = x;
    m.sizeY = y;
    m.sizeZ = z;
    m.sizeT = t;
    m.sizeC = 1;
    if (getSizeZ() == 0)
        m.sizeZ = 1;
    if (getSizeT() == 0)
        m.sizeT = 1;
    m.imageCount = getSizeZ() * getSizeT();
    m.rgb = false;
    m.interleaved = false;
    m.indexed = false;
    m.dimensionOrder = "XYZTC";
    switch(dataType) {
        case 1:
        case 2:
            m.pixelType = FormatTools.UINT8;
            break;
        case 4:
            m.pixelType = FormatTools.INT16;
            break;
        case 8:
            m.pixelType = FormatTools.INT32;
            break;
        case 16:
            m.pixelType = FormatTools.FLOAT;
            break;
        case 64:
            m.pixelType = FormatTools.DOUBLE;
            break;
        case 128:
            m.pixelType = FormatTools.UINT8;
            m.sizeC = 3;
            m.rgb = true;
            m.interleaved = true;
            m.dimensionOrder = "XYCZT";
        default:
            throw new FormatException("Unsupported data type: " + dataType);
    }
    LOGGER.info("Populating MetadataStore");
    MetadataStore store = makeFilterMetadata();
    MetadataTools.populatePixels(store, this);
    store.setImageName(imageName, 0);
    if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) {
        store.setImageDescription(description, 0);
        Length sizeX = FormatTools.getPhysicalSizeX(voxelWidth, UNITS.MILLIMETER);
        Length sizeY = FormatTools.getPhysicalSizeY(voxelHeight, UNITS.MILLIMETER);
        Length sizeZ = FormatTools.getPhysicalSizeZ(sliceThickness, UNITS.MILLIMETER);
        if (sizeX != null) {
            store.setPixelsPhysicalSizeX(sizeX, 0);
        }
        if (sizeY != null) {
            store.setPixelsPhysicalSizeY(sizeY, 0);
        }
        if (sizeZ != null) {
            store.setPixelsPhysicalSizeZ(sizeZ, 0);
        }
        store.setPixelsTimeIncrement(new Time(deltaT, UNITS.MILLISECOND), 0);
    }
}

Example 93 with CoreMetadata

use of loci.formats.CoreMetadata in project bioformats by openmicroscopy.

the class AmiraReader method initFile.

/* (non-Javadoc)
   * @see loci.formats.FormatReader#initFile(java.lang.String)
   */
@Override
protected void initFile(String id) throws FormatException, IOException {
    super.initFile(id);
    in = new RandomAccessInputStream(id);
    AmiraParameters parameters = new AmiraParameters(in);
    offsetOfFirstStream = in.getFilePointer();
    LOGGER.info("Populating metadata hashtable");
    addGlobalMeta("Image width", parameters.width);
    addGlobalMeta("Image height", parameters.height);
    addGlobalMeta("Number of planes", parameters.depth);
    addGlobalMeta("Bits per pixel", 8);
    LOGGER.info("Populating core metadata");
    int channelIndex = 1;
    while (parameters.getStreams().get("@" + channelIndex) != null) {
        channelIndex++;
    }
    CoreMetadata m = core.get(0);
    m.sizeX = parameters.width;
    m.sizeY = parameters.height;
    m.sizeZ = parameters.depth;
    m.sizeT = 1;
    m.sizeC = channelIndex - 1;
    m.imageCount = getSizeZ() * getSizeC();
    m.littleEndian = parameters.littleEndian;
    m.dimensionOrder = "XYZCT";
    String streamType = parameters.streamTypes[0].toLowerCase();
    if (streamType.equals("byte")) {
        m.pixelType = FormatTools.UINT8;
    } else if (streamType.equals("short")) {
        m.pixelType = FormatTools.INT16;
        addGlobalMeta("Bits per pixel", 16);
    } else if (streamType.equals("ushort")) {
        m.pixelType = FormatTools.UINT16;
        addGlobalMeta("Bits per pixel", 16);
    } else if (streamType.equals("int")) {
        m.pixelType = FormatTools.INT32;
        addGlobalMeta("Bits per pixel", 32);
    } else if (streamType.equals("float")) {
        m.pixelType = FormatTools.FLOAT;
        addGlobalMeta("Bits per pixel", 32);
    } else {
        LOGGER.warn("Assuming data type is byte");
        m.pixelType = FormatTools.UINT8;
    }
    LOGGER.info("Populating metadata store");
    MetadataStore store = makeFilterMetadata();
    MetadataTools.populatePixels(store, this);
    // The bounding box is the range of the centre of the voxels
    if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) {
        double pixelWidth = (double) (parameters.x1 - parameters.x0) / (parameters.width - 1);
        double pixelHeight = (double) (parameters.y1 - parameters.y0) / (parameters.height - 1);
        // TODO - what is correct setting if single slice?
        double pixelDepth = (double) (parameters.z1 - parameters.z0) / (parameters.depth - 1);
        // Amira does not have a standard form for encoding units, so we just
        // have to assume microns for microscopy data
        addGlobalMeta("Pixels per meter (X)", 1e6 / pixelWidth);
        addGlobalMeta("Pixels per meter (Y)", 1e6 / pixelHeight);
        addGlobalMeta("Pixels per meter (Z)", 1e6 / pixelDepth);
        Length sizeX = FormatTools.getPhysicalSizeX(pixelWidth);
        Length sizeY = FormatTools.getPhysicalSizeY(pixelHeight);
        Length sizeZ = FormatTools.getPhysicalSizeZ(pixelDepth);
        if (sizeX != null) {
            store.setPixelsPhysicalSizeX(sizeX, 0);
        }
        if (sizeY != null) {
            store.setPixelsPhysicalSizeY(sizeY, 0);
        }
        if (sizeZ != null) {
            store.setPixelsPhysicalSizeZ(sizeZ, 0);
        }
    }
    ascii = parameters.ascii;
    ArrayList streamData = (ArrayList) parameters.getStreams().get("@1");
    if (streamData.size() > 2) {
        compression = (String) streamData.get(2);
    }
    initPlaneReader();
    hasPlaneReader = planeReader != null;
    addGlobalMeta("Compression", compression);
    Map params = (Map) parameters.getMap().get("Parameters");
    if (params != null) {
        Map materials = (Map) params.get("Materials");
        if (materials != null) {
            lut = getLookupTable(materials);
            m.indexed = true;
        }
    }
}

Example 94 with CoreMetadata

use of loci.formats.CoreMetadata in project bioformats by openmicroscopy.

the class BDReader method readMetaData.

private IniList readMetaData(String id) throws IOException {
    IniParser parser = new IniParser();
    RandomAccessInputStream idStream = new RandomAccessInputStream(id);
    IniList exp = parser.parseINI(new BufferedReader(new InputStreamReader(idStream, Constants.ENCODING)));
    IniList plate = null;
    IniList xyz = null;
    // Read Plate File
    for (String filename : metadataFiles) {
        if (checkSuffix(filename, "plt")) {
            RandomAccessInputStream stream = new RandomAccessInputStream(filename);
            plate = parser.parseINI(new BufferedReader(new InputStreamReader(stream, Constants.ENCODING)));
            stream.close();
        } else if (checkSuffix(filename, "xyz")) {
            RandomAccessInputStream stream = new RandomAccessInputStream(filename);
            xyz = parser.parseINI(new BufferedReader(new InputStreamReader(stream, Constants.ENCODING)));
            stream.close();
        } else if (filename.endsWith("RoiSummary.txt")) {
            roiFile = filename;
            if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) {
                RandomAccessInputStream s = new RandomAccessInputStream(filename);
                String line = s.readLine().trim();
                while (!line.endsWith(".adf\"")) {
                    line = s.readLine().trim();
                }
                plateName = line.substring(line.indexOf(':')).trim();
                plateName = plateName.replace('/', File.separatorChar);
                plateName = plateName.replace('\\', File.separatorChar);
                for (int i = 0; i < 3; i++) {
                    plateName = plateName.substring(0, plateName.lastIndexOf(File.separator));
                }
                plateName = plateName.substring(plateName.lastIndexOf(File.separator) + 1);
                s.close();
            }
        }
    }
    if (plate == null)
        throw new IOException("No Plate File");
    IniTable plateType = plate.getTable("PlateType");
    if (plateName == null) {
        plateName = plateType.get("Brand");
    }
    plateDescription = plateType.get("Brand") + " " + plateType.get("Description");
    int nWells = Integer.parseInt(plateType.get("Wells"));
    if (nWells == 96) {
        wellRows = 8;
        wellCols = 12;
    } else if (nWells == 384) {
        wellRows = 16;
        wellCols = 24;
    }
    for (String filename : rootList) {
        String name = new Location(filename).getName();
        if (name.startsWith("Well ")) {
            wellLabels.add(name.split("\\s|\\.")[1]);
        }
    }
    IniTable imageTable = exp.getTable("Image");
    boolean montage = imageTable.get("Montaged").equals("1");
    if (montage) {
        fieldRows = Integer.parseInt(imageTable.get("TilesY"));
        fieldCols = Integer.parseInt(imageTable.get("TilesX"));
    } else {
        fieldRows = 1;
        fieldCols = 1;
    }
    core.clear();
    int coresize = wellLabels.size() * fieldRows * fieldCols;
    CoreMetadata ms0 = new CoreMetadata();
    core.add(ms0);
    for (int i = 1; i < coresize; i++) {
        core.add(new CoreMetadata());
    }
    ms0.sizeC = Integer.parseInt(exp.getTable("General").get("Dyes"));
    ms0.bitsPerPixel = Integer.parseInt(exp.getTable("Camera").get("BitdepthUsed"));
    IniTable dyeTable = exp.getTable("Dyes");
    for (int i = 1; i <= getSizeC(); i++) {
        channelNames.add(dyeTable.get(Integer.toString(i)));
    }
    if (xyz != null) {
        IniTable zTable = xyz.getTable("Z1Axis");
        boolean zEnabled = "1".equals(zTable.get("Z1AxisEnabled")) && "1".equals(zTable.get("Z1AxisMode"));
        if (zEnabled) {
            ms0.sizeZ = (int) Double.parseDouble(zTable.get("Z1AxisValue")) + 1;
        } else {
            ms0.sizeZ = 1;
        }
    } else {
        ms0.sizeZ = 1;
    }
    // Count Images
    ms0.sizeT = 0;
    for (String channelName : channelNames) {
        int images = 0;
        for (String filename : wellList.get(1)) {
            if (filename.startsWith(channelName) && filename.endsWith(".tif")) {
                images++;
            }
        }
        if (images > getImageCount()) {
            ms0.sizeT = images / getSizeZ();
            ms0.imageCount = getSizeZ() * getSizeT() * channelNames.size();
        }
    }
    idStream.close();
    return exp;
}

Example 95 with CoreMetadata

use of loci.formats.CoreMetadata in project bioformats by openmicroscopy.

the class BDReader method initFile.

// -- Internal FormatReader API methods --
/* @see loci.formats.FormatReader#initFile(String) */
@Override
protected void initFile(String id) throws FormatException, IOException {
    // make sure we have the experiment file
    id = locateExperimentFile(id);
    super.initFile(id);
    Location dir = new Location(id).getAbsoluteFile().getParentFile();
    rootList = dir.list(true);
    Arrays.sort(rootList);
    for (int i = 0; i < rootList.length; i++) {
        String file = rootList[i];
        Location f = new Location(dir, file);
        rootList[i] = f.getAbsolutePath();
        if (!f.isDirectory()) {
            if (checkSuffix(file, META_EXT) && !f.isDirectory()) {
                metadataFiles.add(f.getAbsolutePath());
            }
        } else {
            String[] wells = f.list(true);
            Arrays.sort(wells);
            wellList.add(wells);
            for (String well : wells) {
                Location wellFile = new Location(f, well);
                if (!wellFile.isDirectory()) {
                    if (checkSuffix(well, META_EXT)) {
                        metadataFiles.add(wellFile.getAbsolutePath());
                    }
                }
            }
        }
    }
    // parse Experiment metadata
    IniList experiment = readMetaData(id);
    if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) {
        objective = experiment.getTable("Geometry").get("Name");
        IniTable camera = experiment.getTable("Camera");
        binning = camera.get("BinX") + "x" + camera.get("BinY");
        parseChannelData(dir);
        addGlobalMeta("Objective", objective);
        addGlobalMeta("Camera binning", binning);
    }
    final List<String> uniqueRows = new ArrayList<String>();
    final List<String> uniqueColumns = new ArrayList<String>();
    for (String well : wellLabels) {
        String row = well.substring(0, 1).trim();
        String column = well.substring(1).trim();
        if (!uniqueRows.contains(row) && row.length() > 0)
            uniqueRows.add(row);
        if (!uniqueColumns.contains(column) && column.length() > 0) {
            uniqueColumns.add(column);
        }
    }
    int nSlices = getSizeZ() == 0 ? 1 : getSizeZ();
    int nTimepoints = getSizeT();
    int nWells = wellLabels.size();
    int nChannels = getSizeC() == 0 ? channelNames.size() : getSizeC();
    if (nChannels == 0)
        nChannels = 1;
    tiffs = getTiffs();
    reader = new MinimalTiffReader();
    reader.setId(tiffs[0][0]);
    int sizeX = reader.getSizeX();
    int sizeY = reader.getSizeY();
    int pixelType = reader.getPixelType();
    boolean rgb = reader.isRGB();
    boolean interleaved = reader.isInterleaved();
    boolean indexed = reader.isIndexed();
    boolean littleEndian = reader.isLittleEndian();
    if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) {
        IniParser parser = new IniParser();
        for (String metadataFile : metadataFiles) {
            String filename = new Location(metadataFile).getName();
            if (!checkSuffix(metadataFile, new String[] { "txt", "bmp", "adf", "roi" })) {
                String data = DataTools.readFile(metadataFile);
                IniList ini = parser.parseINI(new BufferedReader(new StringReader(data)));
                HashMap<String, String> h = ini.flattenIntoHashMap();
                for (String key : h.keySet()) {
                    addGlobalMeta(filename + " " + key, h.get(key));
                }
            }
        }
    }
    int coresize = core.size();
    core.clear();
    for (int i = 0; i < coresize; i++) {
        CoreMetadata ms = new CoreMetadata();
        core.add(ms);
        ms.sizeC = nChannels;
        ms.sizeZ = nSlices;
        ms.sizeT = nTimepoints;
        ms.sizeX = sizeX / fieldCols;
        ms.sizeY = sizeY / fieldRows;
        ms.pixelType = pixelType;
        ms.rgb = rgb;
        ms.interleaved = interleaved;
        ms.indexed = indexed;
        ms.littleEndian = littleEndian;
        ms.dimensionOrder = "XYZTC";
        ms.imageCount = nSlices * nTimepoints * nChannels;
    }
    MetadataStore store = makeFilterMetadata();
    boolean populatePlanes = getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM;
    MetadataTools.populatePixels(store, this, populatePlanes);
    String plateAcqID = MetadataTools.createLSID("PlateAcquisition", 0, 0);
    store.setPlateAcquisitionID(plateAcqID, 0, 0);
    PositiveInteger fieldCount = FormatTools.getMaxFieldCount(fieldRows * fieldCols);
    if (fieldCount != null) {
        store.setPlateAcquisitionMaximumFieldCount(fieldCount, 0, 0);
    }
    for (int row = 0; row < wellRows; row++) {
        for (int col = 0; col < wellCols; col++) {
            int index = row * wellCols + col;
            store.setWellID(MetadataTools.createLSID("Well", 0, index), 0, index);
            store.setWellRow(new NonNegativeInteger(row), 0, index);
            store.setWellColumn(new NonNegativeInteger(col), 0, index);
        }
    }
    for (int i = 0; i < getSeriesCount(); i++) {
        int well = i / (fieldRows * fieldCols);
        int field = i % (fieldRows * fieldCols);
        MetadataTools.setDefaultCreationDate(store, tiffs[well][0], i);
        String name = wellLabels.get(well);
        String row = name.substring(0, 1);
        Integer col = Integer.parseInt(name.substring(1));
        int index = (row.charAt(0) - 'A') * wellCols + col - 1;
        String wellSampleID = MetadataTools.createLSID("WellSample", 0, index, field);
        store.setWellSampleID(wellSampleID, 0, index, field);
        store.setWellSampleIndex(new NonNegativeInteger(i), 0, index, field);
        String imageID = MetadataTools.createLSID("Image", i);
        store.setWellSampleImageRef(imageID, 0, index, field);
        store.setImageID(imageID, i);
        store.setImageName(name + " Field #" + (field + 1), i);
        store.setPlateAcquisitionWellSampleRef(wellSampleID, 0, 0, i);
    }
    MetadataLevel level = getMetadataOptions().getMetadataLevel();
    if (level != MetadataLevel.MINIMUM) {
        String instrumentID = MetadataTools.createLSID("Instrument", 0);
        store.setInstrumentID(instrumentID, 0);
        String objectiveID = MetadataTools.createLSID("Objective", 0, 0);
        store.setObjectiveID(objectiveID, 0, 0);
        if (objective != null) {
            String[] tokens = objective.split(" ");
            String mag = tokens[0].replaceAll("[xX]", "");
            String na = null;
            int naIndex = 0;
            for (int i = 0; i < tokens.length; i++) {
                if (tokens[i].equals("NA")) {
                    naIndex = i + 1;
                    na = tokens[naIndex];
                    break;
                }
            }
            Double magnification = new Double(mag);
            store.setObjectiveNominalMagnification(magnification, 0, 0);
            if (na != null) {
                na = na.substring(0, 1) + "." + na.substring(1);
                store.setObjectiveLensNA(new Double(na), 0, 0);
            }
            if (naIndex + 1 < tokens.length) {
                store.setObjectiveManufacturer(tokens[naIndex + 1], 0, 0);
            }
        }
        // populate LogicalChannel data
        for (int i = 0; i < getSeriesCount(); i++) {
            store.setImageInstrumentRef(instrumentID, i);
            store.setObjectiveSettingsID(objectiveID, i);
            for (int c = 0; c < getSizeC(); c++) {
                store.setChannelName(channelNames.get(c), i, c);
                Length emission = FormatTools.getEmissionWavelength(emWave[c]);
                Length excitation = FormatTools.getExcitationWavelength(exWave[c]);
                if (emission != null) {
                    store.setChannelEmissionWavelength(emission, i, c);
                }
                if (excitation != null) {
                    store.setChannelExcitationWavelength(excitation, i, c);
                }
                String detectorID = MetadataTools.createLSID("Detector", 0, c);
                store.setDetectorID(detectorID, 0, c);
                store.setDetectorSettingsID(detectorID, i, c);
                store.setDetectorSettingsGain(gain[c], i, c);
                store.setDetectorSettingsOffset(offset[c], i, c);
                store.setDetectorSettingsBinning(getBinning(binning), i, c);
            }
            long firstPlane = 0;
            for (int p = 0; p < getImageCount(); p++) {
                int[] zct = getZCTCoords(p);
                store.setPlaneExposureTime(new Time(exposure[zct[1]], UNITS.SECOND), i, p);
                String file = getFilename(i, p);
                if (file != null) {
                    long plane = getTimestamp(file);
                    if (p == 0) {
                        firstPlane = plane;
                    }
                    double timestamp = (plane - firstPlane) / 1000.0;
                    store.setPlaneDeltaT(new Time(timestamp, UNITS.SECOND), i, p);
                }
            }
        }
        store.setPlateID(MetadataTools.createLSID("Plate", 0), 0);
        store.setPlateRowNamingConvention(getNamingConvention("Letter"), 0);
        store.setPlateColumnNamingConvention(getNamingConvention("Number"), 0);
        store.setPlateName(plateName, 0);
        store.setPlateDescription(plateDescription, 0);
        if (level != MetadataLevel.NO_OVERLAYS) {
            parseROIs(store);
        }
    }
}