Examples with BioAssay ubic.gemma.model.expression.bioAssay.BioAssay used on opensource projects

Example 86 with BioAssay

use of ubic.gemma.model.expression.bioAssay.BioAssay in project Gemma by PavlidisLab.

the class SubsettedAnalysis3Test method test.

@Test
public void test() {
    ee = expressionExperimentService.thawLite(ee);
    Collection<ExperimentalFactor> factors = ee.getExperimentalDesign().getExperimentalFactors();
    assertEquals(3, factors.size());
    for (BioAssay ba : ee.getBioAssays()) {
        assertEquals(3, ba.getSampleUsed().getFactorValues().size());
    }
    ExperimentalFactor organismpart = null;
    ExperimentalFactor disease = null;
    ExperimentalFactor diseasegroup = null;
    for (ExperimentalFactor ef : factors) {
        switch(ef.getCategory().getValue()) {
            case "study design":
                diseasegroup = ef;
                break;
            case "disease":
                disease = ef;
                break;
            case "organism part":
                organismpart = ef;
                break;
        }
    }
    assertNotNull(diseasegroup);
    assertNotNull(disease);
    assertNotNull(organismpart);
    DifferentialExpressionAnalysisConfig config = new DifferentialExpressionAnalysisConfig();
    config.getFactorsToInclude().add(disease);
    config.getFactorsToInclude().add(organismpart);
    config.setSubsetFactor(diseasegroup);
    Collection<DifferentialExpressionAnalysis> analyses = analyzer.run(ee, config);
    // a subset for each disease: SZ, PD, HD, ALS, ALZ, MS
    assertEquals(6, analyses.size());
    /*
         * Now, within each we should have only one disease contrast,
         */
    for (DifferentialExpressionAnalysis analysis : analyses) {
        // there should be one for disease - tissue isn't used.
        assertEquals(1, analysis.getResultSets().size());
        for (ExpressionAnalysisResultSet rs : analysis.getResultSets()) {
            ExperimentalFactor factor = rs.getExperimentalFactors().iterator().next();
            // noinspection LoopStatementThatDoesntLoop
            for (DifferentialExpressionAnalysisResult res : rs.getResults()) {
                Collection<ContrastResult> contrasts = res.getContrasts();
                for (ContrastResult cr : contrasts) {
                    log.info(analysis + "   " + factor + " " + cr + " " + res);
                }
                break;
            }
        }
    }
}

Example 87 with BioAssay

use of ubic.gemma.model.expression.bioAssay.BioAssay in project Gemma by PavlidisLab.

the class ProcessedExpressionDataCreateServiceTest method testReorder.

@Test
public void testReorder() throws Exception {
    ExpressionExperiment old = eeService.findByShortName("GSE404");
    if (old != null) {
        eeService.remove(old);
    }
    try {
        geoService.setGeoDomainObjectGenerator(new GeoDomainObjectGeneratorLocal(this.getTestFileBasePath("gse404Short")));
        @SuppressWarnings("unchecked") Collection<ExpressionExperiment> results = (Collection<ExpressionExperiment>) geoService.fetchAndLoad("GSE404", false, true, false);
        this.ee = results.iterator().next();
    } catch (AlreadyExistsInSystemException e) {
        this.ee = (ExpressionExperiment) e.getData();
    }
    ee = this.eeService.thawLite(ee);
    processedExpressionDataVectorService.computeProcessedExpressionData(ee);
    ExperimentalFactor factor = ExperimentalFactor.Factory.newInstance();
    factor.setType(FactorType.CATEGORICAL);
    factor.setName(ee.getShortName() + " design");
    factor.setExperimentalDesign(ee.getExperimentalDesign());
    factor = eeService.addFactor(ee, factor);
    FactorValue fv1 = FactorValue.Factory.newInstance();
    FactorValue fv2 = FactorValue.Factory.newInstance();
    fv1.setValue("foo");
    fv1.setExperimentalFactor(factor);
    fv2.setValue("bar");
    fv2.setIsBaseline(true);
    fv2.setExperimentalFactor(factor);
    eeService.addFactorValue(ee, fv1);
    eeService.addFactorValue(ee, fv2);
    List<BioAssay> basInOrder = new ArrayList<>(ee.getBioAssays());
    Collections.sort(basInOrder, new Comparator<BioAssay>() {

        @Override
        public int compare(BioAssay o1, BioAssay o2) {
            return o1.getId().compareTo(o2.getId());
        }
    });
    int i = 0;
    for (BioAssay ba : basInOrder) {
        // bioAssayService.thawRawAndProcessed( ba );
        BioMaterial bm = ba.getSampleUsed();
        assert fv1.getId() != null;
        if (!bm.getFactorValues().isEmpty()) {
            continue;
        }
        if (i % 2 == 0) {
            bm.getFactorValues().add(fv1);
        // log.info( bm + " " + bm.getId() + " => " + fv1 );
        } else {
            bm.getFactorValues().add(fv2);
        // log.info( bm + " " + bm.getId() + " => " + fv2 );
        }
        bioMaterialService.update(bm);
        i++;
    }
    factor = this.experimentalFactorService.load(factor.getId());
    assertEquals(2, factor.getFactorValues().size());
    /*
         * All that was setup. Now do the interesting bit
         */
    processedExpressionDataVectorService.reorderByDesign(ee.getId());
    /*
         * Now check the vectors...
         */
    Collection<ProcessedExpressionDataVector> resortedVectors = processedExpressionDataVectorService.getProcessedDataVectors(ee);
    // ExpressionDataDoubleMatrix newMat = new ExpressionDataDoubleMatrix( resortedVectors );
    // log.info( newMat );
    boolean foundVector = false;
    assertTrue(resortedVectors.size() > 0);
    for (ProcessedExpressionDataVector vector : resortedVectors) {
        i = 0;
        log.debug(vector.getDesignElement().getName() + " .........................");
        // thawingto avoid lazy error because we are outside of transaction in this test. All references in code run
        // inside a transaction
        BioAssayDimension bioAssayDimension = vector.getBioAssayDimension();
        bioAssayDimensionService.thawLite(bioAssayDimension);
        Collection<BioAssay> bioAssays = bioAssayDimension.getBioAssays();
        for (BioAssay ba : bioAssays) {
            BioMaterial bm = ba.getSampleUsed();
            assertEquals(1, bm.getFactorValues().size());
            FactorValue fv = bm.getFactorValues().iterator().next();
            assertNotNull(fv.getId());
            log.debug(ba.getId() + " " + fv.getId() + " " + fv);
            if (i < 10) {
                // first because it is baseline;
                assertEquals(fv2, fv);
            }
            i++;
        }
        /*
             * spot check the data, same place as before.
             */
        if (vector.getDesignElement().getName().equals("40")) {
            foundVector = true;
            ByteArrayConverter conv = new ByteArrayConverter();
            Double[] d = ArrayUtils.toObject(conv.byteArrayToDoubles(vector.getData()));
            assertEquals(20, d.length);
            assertEquals(-0.08, d[1], 0.001);
            assertEquals(0.45, d[10], 0.001);
            assertEquals(Double.NaN, d[19], 0.001);
        }
    }
    assertTrue("test vector not found", foundVector);
}

Example 88 with BioAssay

use of ubic.gemma.model.expression.bioAssay.BioAssay in project Gemma by PavlidisLab.

the class ProcessedExpressionDataCreateServiceTest method testComputeDevRankForExpressionExperimentMultiArrayWithGaps.

/**
 * Three platforms, one sample was not run on GPL81. It's 'Norm-1a', but the name we use for the sample is random.
 */
@SuppressWarnings("unchecked")
@Test
public void testComputeDevRankForExpressionExperimentMultiArrayWithGaps() throws Exception {
    try {
        geoService.setGeoDomainObjectGenerator(new GeoDomainObjectGeneratorLocal(this.getTestFileBasePath("gse482short")));
        Collection<ExpressionExperiment> results = (Collection<ExpressionExperiment>) geoService.fetchAndLoad("GSE482", false, true, false);
        this.ee = results.iterator().next();
    } catch (AlreadyExistsInSystemException e) {
        this.ee = ((Collection<ExpressionExperiment>) e.getData()).iterator().next();
    }
    ee = this.eeService.thawLite(ee);
    processedExpressionDataVectorService.computeProcessedExpressionData(ee);
    Collection<ProcessedExpressionDataVector> preferredVectors = this.processedExpressionDataVectorService.getProcessedDataVectors(ee);
    ee = eeService.load(ee.getId());
    ee = this.eeService.thawLite(ee);
    processedExpressionDataVectorService.thaw(preferredVectors);
    ExpressionDataDoubleMatrix mat = new ExpressionDataDoubleMatrix(preferredVectors);
    assertEquals(10, mat.columns());
    boolean found = false;
    for (int i = 0; i < mat.rows(); i++) {
        Double[] row = mat.getRow(i);
        // debugging
        if (i == 0) {
            for (int j = 0; j < row.length; j++) {
                BioAssay ba = mat.getBioAssaysForColumn(j).iterator().next();
                System.err.println(ba.getName());
            }
        }
        System.err.print(mat.getRowElement(i).getDesignElement().getName() + "\t");
        for (double d : row) {
            System.err.print(String.format("%4.2f\t", d));
        }
        System.err.print("\n");
        CompositeSequence el = mat.getDesignElementForRow(i);
        for (int j = 0; j < row.length; j++) {
            BioAssay ba = mat.getBioAssaysForColumn(j).iterator().next();
            if (ba.getName().matches("PGA-MurLungHyper-Norm-1a[ABC]v2-s2") && (el.getName().equals("100001_at") || el.getName().equals("100002_at") || el.getName().equals("100003_at") || el.getName().equals("100004_at") || el.getName().equals("100005_at") || el.getName().equals("100006_at") || el.getName().equals("100007_at") || el.getName().equals("100009_r_at") || el.getName().equals("100010_at") || el.getName().equals("100011_at"))) {
                assertEquals(Double.NaN, row[j], 0.0001);
                found = true;
            } else {
                assertTrue("Got unexpected NA value for " + ba.getName() + " for " + el.getName(), !Double.isNaN(row[j]));
            }
        }
    }
    assertTrue(found);
    /*
         * Now do this through the processedExpressionDataVectorService
         */
    Collection<DoubleVectorValueObject> da = this.processedExpressionDataVectorService.getProcessedDataArrays(ee);
    assertEquals(30, da.size());
    found = false;
    boolean first = true;
    for (DoubleVectorValueObject v : da) {
        CompositeSequenceValueObject el = v.getDesignElement();
        double[] row = v.getData();
        // debugging
        if (first) {
            for (int j = 0; j < row.length; j++) {
                BioAssayValueObject ba = v.getBioAssays().get(j);
                System.err.println(ba.getName());
            }
            first = false;
        }
        System.err.print(el.getName() + "\t");
        for (double d : row) {
            System.err.print(String.format("%4.2f\t", d));
        }
        System.err.print("\n");
        assertEquals(10, row.length);
        for (int j = 0; j < row.length; j++) {
            assertNotNull(v.getBioAssays());
            BioAssayValueObject ba = v.getBioAssays().get(j);
            if (ba.getName().startsWith("Missing bioassay for biomaterial") && (el.getName().equals("100001_at") || el.getName().equals("100002_at") || el.getName().equals("100003_at") || el.getName().equals("100004_at") || el.getName().equals("100005_at") || el.getName().equals("100006_at") || el.getName().equals("100007_at") || el.getName().equals("100009_r_at") || el.getName().equals("100010_at") || el.getName().equals("100011_at"))) {
                assertEquals(Double.NaN, row[j], 0.0001);
                found = true;
            } else {
                assertTrue("Got unexpected NA value for " + ba.getName() + " for " + el.getName(), !Double.isNaN(row[j]));
            }
        }
    }
    assertTrue(found);
}

Example 89 with BioAssay

use of ubic.gemma.model.expression.bioAssay.BioAssay in project Gemma by PavlidisLab.

the class VectorMergingServiceTest method test.

@Test
public final void test() throws Exception {
    /*
         * Need a persistent experiment that uses multiple array designs. Then merge the designs, switch the vectors,
         * and merge the vectors. GSE3443
         */
    /*
         * The experiment uses the following GPLs
         *
         * GPL2868, GPL2933, GPL2934, GPL2935, GPL2936, GPL2937, GPL2938
         *
         * Example of a sequence appearing on more than one platform: N57553
         */
    geoService.setGeoDomainObjectGenerator(new GeoDomainObjectGeneratorLocal(this.getTestFileBasePath("gse3443merge")));
    Collection<?> results = geoService.fetchAndLoad("GSE3443", false, false, false);
    ee = (ExpressionExperiment) results.iterator().next();
    ee = this.eeService.thawLite(ee);
    Collection<ArrayDesign> aas = eeService.getArrayDesignsUsed(ee);
    assertEquals(7, aas.size());
    /*
         * Check number of sequences across all platforms. This is how many elements we need on the new platform, plus
         * extras for duplicated sequences (e.g. elements that don't have a sequence...)
         */
    Collection<ArrayDesign> taas = new HashSet<>();
    Set<BioSequence> oldbs = new HashSet<>();
    for (ArrayDesign arrayDesign : aas) {
        arrayDesign = arrayDesignService.thaw(arrayDesign);
        taas.add(arrayDesign);
        for (CompositeSequence cs : arrayDesign.getCompositeSequences()) {
            log.info(cs + " " + cs.getBiologicalCharacteristic());
            oldbs.add(cs.getBiologicalCharacteristic());
        }
    }
    assertEquals(63, oldbs.size());
    /*
         * Check total size of elements across all 7 platforms.
         */
    int totalElements = 0;
    for (ArrayDesign arrayDesign : taas) {
        totalElements += arrayDesign.getCompositeSequences().size();
    }
    assertEquals(140, totalElements);
    ArrayDesign firstaa = taas.iterator().next();
    aas.remove(firstaa);
    assertEquals(null, firstaa.getMergedInto());
    mergedAA = arrayDesignMergeService.merge(firstaa, taas, "testMerge" + RandomStringUtils.randomAlphabetic(5), "merged" + RandomStringUtils.randomAlphabetic(5), false);
    assertEquals(72, mergedAA.getCompositeSequences().size());
    Set<BioSequence> seenBs = new HashSet<>();
    for (CompositeSequence cs : mergedAA.getCompositeSequences()) {
        seenBs.add(cs.getBiologicalCharacteristic());
    }
    assertEquals(63, seenBs.size());
    // just to make this explicit. The new array design has to contain all the old sequences.
    assertEquals(oldbs.size(), seenBs.size());
    ee = eeService.thaw(ee);
    assertEquals(1828, ee.getRawExpressionDataVectors().size());
    ee = eePlatformSwitchService.switchExperimentToArrayDesign(ee, mergedAA);
    ee = eeService.thaw(ee);
    // check we actually got switched over.
    for (BioAssay ba : ee.getBioAssays()) {
        assertEquals(mergedAA, ba.getArrayDesignUsed());
    }
    for (RawExpressionDataVector v : ee.getRawExpressionDataVectors()) {
        assertEquals(mergedAA, v.getDesignElement().getArrayDesign());
    }
    assertEquals(15, ee.getQuantitationTypes().size());
    assertEquals(1828, ee.getRawExpressionDataVectors().size());
    ee = vectorMergingService.mergeVectors(ee);
    // check we got the right processed data
    Collection<ProcessedExpressionDataVector> pvs = processedExpressionDataVectorService.getProcessedDataVectors(ee);
    assertEquals(72, pvs.size());
    ee = eeService.thaw(ee);
    Collection<DoubleVectorValueObject> processedDataArrays = processedExpressionDataVectorService.getProcessedDataArrays(ee, 50);
    assertEquals(50, processedDataArrays.size());
}

Example 90 with BioAssay

use of ubic.gemma.model.expression.bioAssay.BioAssay in project Gemma by PavlidisLab.

the class CharacteristicServiceTest method setup.

@Before
public void setup() {
    ee = this.getTestPersistentBasicExpressionExperiment();
    ee.setCharacteristics(this.getTestPersistentCharacteristics(2));
    Characteristic[] eeChars = ee.getCharacteristics().toArray(new Characteristic[0]);
    eeChar1 = eeChars[0];
    eeChar2 = eeChars[1];
    eeService.update(ee);
    BioAssay ba = ee.getBioAssays().toArray(new BioAssay[0])[0];
    BioMaterial bm = ba.getSampleUsed();
    bm.setCharacteristics(this.getTestPersistentCharacteristics(1));
    bmService.update(bm);
    for (ExperimentalFactor ef : testHelper.getExperimentalFactors(ee.getExperimentalDesign())) {
        eeService.addFactor(ee, ef);
    }
    ExperimentalFactor ef = ee.getExperimentalDesign().getExperimentalFactors().iterator().next();
    for (FactorValue f : testHelper.getFactorValues(ef)) {
        eeService.addFactorValue(ee, f);
    }
    FactorValue fv = ef.getFactorValues().iterator().next();
    fv.setCharacteristics(this.getTestPersistentCharacteristics(1));
    fvService.update(fv);
}