Examples with OtherCanonicalAlign com.github.lindenb.jvarkit.util.picard.OtherCanonicalAlign used on opensource projects

Example 1 with OtherCanonicalAlign

use of com.github.lindenb.jvarkit.util.picard.OtherCanonicalAlign in project jvarkit by lindenb.

the class BWAMemDigest method doWork.

@Override
public int doWork(List<String> args) {
    IntervalTreeMap<Boolean> ignore = null;
    DefaultMemOuput output = new DefaultMemOuput();
    final float limitcigar = 0.15f;
    SamReader r = null;
    try {
        r = super.openSamReader(oneFileOrNull(args));
        SAMFileHeader header = r.getFileHeader();
        if (IGNORE_BED != null) {
            LOG.info("open " + IGNORE_BED);
            ignore = new IntervalTreeMap<>();
            BufferedReader in = IOUtils.openFileForBufferedReading(IGNORE_BED);
            String line;
            while ((line = in.readLine()) != null) {
                if (line.isEmpty() || line.startsWith("#"))
                    continue;
                String[] tokens = line.split("[\t]");
                if (tokens.length < 3)
                    continue;
                if (ignore.put(new Interval(tokens[0], Math.max(1, Integer.parseInt(tokens[1]) - (1 + IGNORE_EXTEND)), Integer.parseInt(tokens[2]) + IGNORE_EXTEND), Boolean.TRUE)) {
                    LOG.warn("BED:ignoring " + line);
                }
            }
            in.close();
        }
        OtherCanonicalAlignFactory xPalignFactory = new OtherCanonicalAlignFactory(header);
        SAMRecordIterator iter = r.iterator();
        long readNum = 0L;
        while (iter.hasNext()) {
            SAMRecord record = iter.next();
            ++readNum;
            if (!record.getReadPairedFlag())
                continue;
            if (record.getProperPairFlag())
                continue;
            if (record.getReadFailsVendorQualityCheckFlag())
                continue;
            if (record.getDuplicateReadFlag())
                continue;
            if (record.getReadUnmappedFlag())
                continue;
            if (ignore != null && ignore.containsOverlapping(new Interval(record.getReferenceName(), record.getAlignmentStart(), record.getAlignmentEnd()))) {
                LOG.info("ignore " + record);
                continue;
            }
            float countM = 0f;
            float countS = 0f;
            for (CigarElement c : record.getCigar().getCigarElements()) {
                switch(c.getOperator()) {
                    case M:
                        countM += c.getLength();
                        break;
                    case S:
                        countS += c.getLength();
                        break;
                    default:
                        break;
                }
            }
            if (countM > 10 && ((countS / countM) > (1f - limitcigar) && (countS / countM) < (1f + limitcigar))) {
                output.insertion(record, readNum, countS, countM);
            }
            for (OtherCanonicalAlign xp : xPalignFactory.getXPAligns(record)) {
                if (ignore != null && ignore.containsOverlapping(new Interval(xp.getReferenceName(), xp.getAlignmentStart(), xp.getAlignmentStart()))) {
                    LOG.info("ignore " + record);
                    continue;
                }
                output.xp(record, readNum, xp);
            }
            if (record.getMateUnmappedFlag()) {
                output.orphan(record, readNum);
                continue;
            }
            if (ignore != null && ignore.containsOverlapping(new Interval(record.getMateReferenceName(), record.getMateAlignmentStart(), record.getMateAlignmentStart()))) {
                LOG.info("ignore " + record);
                continue;
            }
            if (record.getReferenceIndex() == record.getMateReferenceIndex()) {
                output.deletion(record, readNum);
            } else {
                output.transloc(record, readNum);
            }
        }
    } catch (Exception e) {
        e.printStackTrace();
        LOG.error(e);
        return -1;
    } finally {
        CloserUtil.close(r);
        CloserUtil.close(output);
    }
    return 0;
}

Example 2 with OtherCanonicalAlign

use of com.github.lindenb.jvarkit.util.picard.OtherCanonicalAlign in project jvarkit by lindenb.

the class BWAMemNOp method doWork.

@Override
public int doWork(List<String> args) {
    SamReader r = null;
    SAMFileWriter w = null;
    try {
        r = super.openSamReader(oneFileOrNull(args));
        SAMFileHeader header = r.getFileHeader();
        OtherCanonicalAlignFactory ocaf = new OtherCanonicalAlignFactory(header);
        w = writingBamArgs.openSAMFileWriter(outputFile, header, true);
        SAMRecordFactory samRecordFactory = new DefaultSAMRecordFactory();
        SAMRecordIterator iter = r.iterator();
        while (iter.hasNext()) {
            SAMRecord rec = iter.next();
            if (rec.getSupplementaryAlignmentFlag()) {
                continue;
            }
            if (rec.getReadUnmappedFlag()) {
                if (!print_only_spit_read)
                    w.addAlignment(rec);
                continue;
            }
            Cigar cigar1 = rec.getCigar();
            if (cigar1 == null || cigar1.isEmpty() || !(cigar1.getCigarElement(cigar1.numCigarElements() - 1).getOperator().equals(CigarOperator.S) || cigar1.getCigarElement(0).getOperator().equals(CigarOperator.S))) // last or first is soft clipping
            {
                if (!print_only_spit_read)
                    w.addAlignment(rec);
                continue;
            }
            rec.getAlignmentStart();
            List<OtherCanonicalAlign> xps = ocaf.getXPAligns(rec);
            if (xps.isEmpty()) {
                if (!print_only_spit_read)
                    w.addAlignment(rec);
                continue;
            }
            boolean found_one = false;
            for (OtherCanonicalAlign xp : xps) {
                if (!rec.getReferenceName().equals(xp.getReferenceName()))
                    continue;
                if (xp.getReadNegativeStrandFlag() != rec.getReadNegativeStrandFlag())
                    continue;
                Cigar cigar2 = xp.getCigar();
                if (cigar2 == null || cigar2.isEmpty()) {
                    continue;
                }
                SAMRecord newrec = null;
                List<CigarEvt> L1 = null;
                List<CigarEvt> L2 = null;
                if (cigar1.getCigarElement(cigar1.numCigarElements() - 1).getOperator().equals(CigarOperator.S) && cigar1.getCigarElement(cigar1.numCigarElements() - 1).getLength() >= this.min_soft_clip_length && cigar2.getCigarElement(0).getOperator().equals(CigarOperator.S) && cigar2.getCigarElement(0).getLength() >= this.min_soft_clip_length && rec.getAlignmentEnd() < xp.getAlignmentStart()) {
                    newrec = samRecordFactory.createSAMRecord(header);
                    int ref1 = rec.getAlignmentStart();
                    newrec.setAlignmentStart(ref1);
                    L1 = cigarEvents(0, ref1, cigar1);
                    L2 = cigarEvents(0, xp.getAlignmentStart(), cigar2);
                } else if (cigar2.getCigarElement(cigar2.numCigarElements() - 1).getOperator().equals(CigarOperator.S) && cigar2.getCigarElement(cigar2.numCigarElements() - 1).getLength() >= this.min_soft_clip_length && cigar1.getCigarElement(0).getOperator().equals(CigarOperator.S) && cigar1.getCigarElement(0).getLength() >= this.min_soft_clip_length && xp.getAlignmentEnd() < rec.getAlignmentStart()) {
                    newrec = samRecordFactory.createSAMRecord(header);
                    int ref1 = xp.getAlignmentStart();
                    newrec.setAlignmentStart(ref1);
                    L1 = cigarEvents(0, ref1, cigar2);
                    L2 = cigarEvents(0, rec.getAlignmentStart(), cigar1);
                }
                if (newrec == null)
                    continue;
                newrec.setFlags(rec.getFlags());
                newrec.setReadName(rec.getReadName());
                newrec.setReadBases(rec.getReadBases());
                newrec.setMappingQuality(rec.getMappingQuality());
                newrec.setReferenceIndex(rec.getReferenceIndex());
                newrec.setBaseQualities(rec.getBaseQualities());
                if (found_one) {
                    newrec.setNotPrimaryAlignmentFlag(true);
                }
                found_one = true;
                for (SAMTagAndValue tav : rec.getAttributes()) {
                    if (tav.tag.equals(ocaf.getAttributeKey()))
                        continue;
                    if (tav.tag.equals("NM"))
                        continue;
                    newrec.setAttribute(tav.tag, tav.value);
                }
                if (rec.getReadPairedFlag() && !rec.getMateUnmappedFlag()) {
                    newrec.setMateAlignmentStart(rec.getMateAlignmentStart());
                    newrec.setMateReferenceIndex(rec.getMateReferenceIndex());
                    newrec.setInferredInsertSize(rec.getInferredInsertSize());
                }
                while (!L1.isEmpty() && (L1.get(L1.size() - 1).op.equals(CigarOperator.S) || L1.get(L1.size() - 1).op.equals(CigarOperator.D) || L1.get(L1.size() - 1).op.equals(CigarOperator.H))) {
                    L1.remove(L1.size() - 1);
                }
                while (!L2.isEmpty() && L2.get(0).read0 <= L1.get(L1.size() - 1).read0) {
                    L2.remove(0);
                }
                List<CigarElement> cigarElements = new ArrayList<CigarElement>();
                int i = 0;
                while (i < L1.size()) {
                    int j = i + 1;
                    while (j < L1.size() && L1.get(i).op.equals(L1.get(j).op)) {
                        j++;
                    }
                    cigarElements.add(new CigarElement(j - i, L1.get(i).op));
                    i = j;
                }
                // add 'N'
                cigarElements.add(new CigarElement((L2.get(0).ref1 - L1.get(L1.size() - 1).ref1) - 1, CigarOperator.N));
                i = 0;
                while (i < L2.size()) {
                    int j = i + 1;
                    while (j < L2.size() && L2.get(i).op.equals(L2.get(j).op)) {
                        j++;
                    }
                    cigarElements.add(new CigarElement(j - i, L2.get(i).op));
                    i = j;
                }
                // cleanup : case where  'S' is close to 'N'
                i = 0;
                while (i + 1 < cigarElements.size()) {
                    CigarElement ce1 = cigarElements.get(i);
                    CigarElement ce2 = cigarElements.get(i + 1);
                    if (i > 0 && ce1.getOperator().equals(CigarOperator.S) && ce2.getOperator().equals(CigarOperator.N)) {
                        cigarElements.set(i, new CigarElement(ce1.getLength(), CigarOperator.X));
                    } else if (i + 2 < cigarElements.size() && ce1.getOperator().equals(CigarOperator.N) && ce2.getOperator().equals(CigarOperator.S)) {
                        cigarElements.set(i + 1, new CigarElement(ce2.getLength(), CigarOperator.X));
                    }
                    i++;
                }
                newrec.setCigar(new Cigar(cigarElements));
                List<SAMValidationError> validations = newrec.isValid();
                if (validations != null) {
                    for (SAMValidationError err : validations) {
                        LOG.warning(err.getType() + ":" + err.getMessage());
                    }
                }
                w.addAlignment(newrec);
            }
            if (!found_one) {
                if (!print_only_spit_read)
                    w.addAlignment(rec);
            }
        }
        iter.close();
        return 0;
    } catch (Exception err) {
        LOG.error(err);
        return -1;
    } finally {
        CloserUtil.close(r);
        CloserUtil.close(w);
    }
}

Example 3 with OtherCanonicalAlign

use of com.github.lindenb.jvarkit.util.picard.OtherCanonicalAlign in project jvarkit by lindenb.

the class FindMyVirus method doWork.

@Override
public int doWork(List<String> args) {
    if (virusNames.isEmpty()) {
        LOG.error("no virus name");
        return -1;
    }
    SamReader sfr = null;
    SAMFileWriter[] sfwArray = new SAMFileWriter[CAT.values().length];
    try {
        sfr = openSamReader(oneFileOrNull(args));
        SAMFileHeader header = sfr.getFileHeader();
        for (CAT category : CAT.values()) {
            LOG.info("Opening " + category);
            SAMFileHeader header2 = header.clone();
            header2.addComment("Category:" + category.name());
            header2.addComment("Description:" + category.getDescription());
            SAMProgramRecord rec = header2.createProgramRecord();
            rec.setCommandLine(this.getProgramCommandLine());
            rec.setProgramName(getProgramName());
            rec.setProgramVersion(getVersion());
            rec.setAttribute("CAT", category.name());
            File outputFile = new File(this.outputFile.getParentFile(), this.outputFile.getName() + "." + category.name() + ".bam");
            LOG.info("Opening " + outputFile);
            File countFile = new File(outputFile.getParentFile(), outputFile.getName() + "." + category.name() + ".count.txt");
            SAMFileWriter sfw = writingBamArgs.openSAMFileWriter(outputFile, header2, true);
            sfw = new SAMFileWriterCount(sfw, countFile, category);
            sfwArray[category.ordinal()] = sfw;
        }
        SAMSequenceDictionaryProgress progress = new SAMSequenceDictionaryProgress(header.getSequenceDictionary());
        OtherCanonicalAlignFactory xpAlignFactory = new OtherCanonicalAlignFactory(header);
        SAMRecordIterator iter = sfr.iterator();
        while (iter.hasNext()) {
            SAMRecord rec = iter.next();
            progress.watch(rec);
            CAT category = null;
            List<OtherCanonicalAlign> xpList = Collections.emptyList();
            if (category == null && !rec.getReadPairedFlag()) {
                category = CAT.unpaired;
            }
            if (category == null && rec.isSecondaryOrSupplementary()) {
                category = CAT.secondary;
            }
            if (category == null && rec.getReadFailsVendorQualityCheckFlag()) {
                category = CAT.failsqual;
            }
            if (category == null && rec.getDuplicateReadFlag()) {
                category = CAT.duplicate;
            }
            if (category == null && rec.getReadUnmappedFlag()) {
                category = CAT.unmapped;
            }
            if (category == null) {
                xpList = xpAlignFactory.getXPAligns(rec);
            }
            boolean xp_containsVirus = false;
            boolean xp_containsChrom = false;
            for (OtherCanonicalAlign xpa : xpList) {
                if (virusNames.contains(xpa.getReferenceName())) {
                    xp_containsVirus = true;
                } else {
                    xp_containsChrom = true;
                }
            }
            /* both reads mapped on ref */
            if (category == null && !rec.getReadUnmappedFlag() && !rec.getMateUnmappedFlag() && !virusNames.contains(rec.getReferenceName()) && !virusNames.contains(rec.getMateReferenceName())) {
                if (!xp_containsVirus) {
                    category = CAT.both_ref;
                } else {
                    category = CAT.ref_and_virus_spliced;
                }
            }
            /*  pair(unmapped,mapped on reference) */
            if (category == null && ((!rec.getReadUnmappedFlag() && rec.getMateUnmappedFlag() && !virusNames.contains(rec.getReferenceName())) || (rec.getReadUnmappedFlag() && !rec.getMateUnmappedFlag() && !virusNames.contains(rec.getMateReferenceName())))) {
                if (!xp_containsVirus) {
                    category = CAT.ref_orphan;
                } else {
                    category = CAT.ref_and_virus_spliced;
                }
            }
            /* both reads mapped on virus */
            if (category == null && !rec.getReadUnmappedFlag() && !rec.getMateUnmappedFlag() && virusNames.contains(rec.getReferenceName()) && virusNames.contains(rec.getMateReferenceName())) {
                if (!xp_containsChrom) {
                    category = CAT.both_virus;
                } else {
                    category = CAT.ref_and_virus_spliced;
                }
            }
            if (category == null && ((!rec.getReadUnmappedFlag() && rec.getMateUnmappedFlag() && virusNames.contains(rec.getReferenceName())) || (rec.getReadUnmappedFlag() && !rec.getMateUnmappedFlag() && virusNames.contains(rec.getMateReferenceName())))) {
                if (!xp_containsChrom) {
                    category = CAT.virus_orphan;
                } else {
                    category = CAT.ref_and_virus_spliced;
                }
            }
            if (category == null && !rec.getReadUnmappedFlag() && !rec.getMateUnmappedFlag() && ((virusNames.contains(rec.getReferenceName()) && !virusNames.contains(rec.getMateReferenceName())) || (!virusNames.contains(rec.getReferenceName()) && virusNames.contains(rec.getMateReferenceName())))) {
                category = CAT.ref_and_virus;
            }
            /*dispatch */
            if (category == null) {
                LOG.warning("Not handled: " + rec);
                category = CAT.undetermined;
            }
            sfwArray[category.ordinal()].addAlignment(rec);
        }
        for (SAMFileWriter sfw : sfwArray) {
            LOG.info("Closing " + sfw);
            sfw.close();
        }
        return 0;
    } catch (Exception err) {
        LOG.error(err);
        return -1;
    } finally {
        LOG.info("Closing");
        CloserUtil.close(sfr);
        CloserUtil.close(sfwArray);
    }
}

Example 4 with OtherCanonicalAlign

use of com.github.lindenb.jvarkit.util.picard.OtherCanonicalAlign in project jvarkit by lindenb.

the class SamShortInvertion method doWork.

@Override
public int doWork(List<String> input) {
    SamReader r = null;
    PrintStream out = null;
    // SAMFileWriter w=null;
    try {
        final List<SamReaderList> samReaders = new ArrayList<>();
        final Set<String> args = IOUtils.unrollFiles(input);
        if (args.isEmpty()) {
            LOG.error("No input file");
            return -1;
        }
        SAMSequenceDictionary dict = null;
        for (final String bam : args) {
            final SamReaderList samReader = new SamReaderList(new File(bam), 2);
            for (int i = 0; i < samReaders.size(); ++i) {
                if (samReaders.get(i).sample.equals(samReader.sample)) {
                    samReader.close();
                    LOG.error("Sample defined in two bams " + samReader.sample);
                    return -1;
                }
                if (dict == null) {
                    dict = samReader.dict;
                } else if (!SequenceUtil.areSequenceDictionariesEqual(dict, samReader.dict)) {
                    samReader.close();
                    LOG.error("bam contains two sequence dict.");
                    return -1;
                }
            }
            samReaders.add(samReader);
        }
        final Predicate<SAMRecord> samRecordFilter = new Predicate<SAMRecord>() {

            @Override
            public boolean test(SAMRecord rec) {
                if (rec.getReadUnmappedFlag())
                    return false;
                if (rec.isSecondaryOrSupplementary())
                    return false;
                if (rec.getDuplicateReadFlag())
                    return false;
                if (rec.getReadFailsVendorQualityCheckFlag())
                    return false;
                return true;
            }
        };
        for (SamReaderList samReader : samReaders) {
            SAMRecordIterator iter = samReader.get(0).iterator();
            while (iter.hasNext()) {
                final SAMRecord rec = iter.next();
                if (!samRecordFilter.test(rec))
                    continue;
                boolean skip = true;
                if (rec.getCigar() != null && rec.getCigar().isClipped()) {
                    skip = false;
                }
                if (skip)
                    continue;
                if (rec.getReadPairedFlag()) {
                    if (!rec.getMateUnmappedFlag()) {
                        SAMRecordIterator iter2 = samReader.get(1).query(rec.getMateReferenceName(), rec.getMateAlignmentStart(), rec.getMateAlignmentStart(), false);
                        while (iter2.hasNext()) {
                            final SAMRecord rec2 = iter2.next();
                            if (!samRecordFilter.test(rec2))
                                continue;
                            if (!rec2.getReadName().equals(rec.getReadName()))
                                continue;
                            if (rec2.getFirstOfPairFlag() == rec.getFirstOfPairFlag())
                                continue;
                            if (rec2.getSecondOfPairFlag() == rec.getSecondOfPairFlag())
                                continue;
                            challenge(rec, rec2);
                            break;
                        }
                        iter2.close();
                    } else {
                        challenge(rec, null);
                    }
                }
            }
            iter.close();
        }
        r = null;
        out = openFileOrStdoutAsPrintStream(outputFile);
        final SAMFileHeader header = r.getFileHeader();
        OtherCanonicalAlignFactory xpalignFactory = new OtherCanonicalAlignFactory(header);
        int prev_tid = -1;
        short[] coverage = null;
        short max_coverage = 0;
        // w=swf.make(header, System.out);
        final SAMSequenceDictionaryProgress progress = new SAMSequenceDictionaryProgress(header.getSequenceDictionary());
        final SAMRecordIterator it = r.iterator();
        for (; ; ) {
            SAMRecord rec = null;
            if (it.hasNext()) {
                rec = progress.watch(it.next());
                if (rec.getReadUnmappedFlag())
                    continue;
                if (rec.isSecondaryOrSupplementary())
                    continue;
                if (rec.getDuplicateReadFlag())
                    continue;
                if (rec.getReadFailsVendorQualityCheckFlag())
                    continue;
            }
            if (rec == null || prev_tid == -1 || (prev_tid != -1 && prev_tid != rec.getReferenceIndex())) {
                if (coverage != null) {
                    while (max_coverage >= Math.max(1, this.min_coverage)) {
                        LOG.info("Scanning " + header.getSequence(prev_tid).getSequenceName() + " for cov:" + max_coverage);
                        int chromStart0 = 0;
                        while (chromStart0 < coverage.length) {
                            if (coverage[chromStart0] == max_coverage) {
                                // reset that pos
                                coverage[chromStart0] = 0;
                                int chromEnd0 = chromStart0 + 1;
                                while (chromEnd0 < coverage.length && coverage[chromEnd0] == max_coverage) {
                                    // reset that pos
                                    coverage[chromEnd0] = 0;
                                    ++chromEnd0;
                                }
                                out.print(header.getSequence(prev_tid).getSequenceName());
                                out.print('\t');
                                out.print(chromStart0);
                                out.print('\t');
                                out.print(chromEnd0);
                                out.print('\t');
                                out.print(max_coverage);
                                out.println();
                                // reset 3'
                                for (int x = chromEnd0; x < coverage.length && coverage[x] > 0; ++x) {
                                    coverage[x] = 0;
                                }
                                // reset 5'
                                for (int x = chromStart0 - 1; x >= 0 && coverage[x] > 0; --x) {
                                    coverage[x] = 0;
                                }
                                chromStart0 = chromEnd0;
                            } else {
                                ++chromStart0;
                            }
                        }
                        max_coverage--;
                    }
                    coverage = null;
                }
                if (rec == null)
                    break;
                prev_tid = rec.getReferenceIndex();
                LOG.info("Alloc sizeof(short)*" + header.getSequence(prev_tid).getSequenceLength());
                coverage = new short[header.getSequence(prev_tid).getSequenceLength()];
                Arrays.fill(coverage, (short) 0);
                max_coverage = 0;
            }
            List<OtherCanonicalAlign> saList = xpalignFactory.getXPAligns(rec);
            if (saList.isEmpty())
                continue;
            for (OtherCanonicalAlign xp : saList) {
                if (!xp.getReferenceName().equals(rec.getReferenceName()))
                    continue;
                if (// read is plus
                !rec.getReadNegativeStrandFlag()) {
                    if (!xp.getReadNegativeStrandFlag()) {
                        // ignore both same strand
                        continue;
                    }
                } else // read.strand=='-'
                {
                    if (xp.getReadNegativeStrandFlag()) {
                        // ignore both same strand
                        continue;
                    }
                }
                if (Math.abs(rec.getUnclippedStart() - xp.getAlignmentStart()) > max_size_inversion) {
                    // info(xp+" vs pos "+rec.getUnclippedStart());
                    continue;
                }
                int chromStart = Math.min(rec.getUnclippedStart(), xp.getAlignmentStart()) - 1;
                int chromEnd = Math.max(rec.getUnclippedEnd(), xp.getAlignmentStart()) - 1;
                for (int x = chromStart; x <= chromEnd && x < coverage.length; ++x) {
                    if (coverage[x] < Short.MAX_VALUE)
                        coverage[x]++;
                    if (max_coverage < coverage[x]) {
                        LOG.info("Max coverage " + max_coverage);
                        max_coverage = coverage[x];
                    }
                }
            }
        }
        it.close();
        r.close();
        r = null;
        progress.finish();
        return RETURN_OK;
    } catch (Exception err) {
        LOG.error(err);
        return -1;
    } finally {
        CloserUtil.close(r);
        CloserUtil.close(out);
    }
}