Examples with SequenceReader com.milaboratory.core.io.sequence.SequenceReader used on opensource projects

Example 1 with SequenceReader

use of com.milaboratory.core.io.sequence.SequenceReader in project mixcr by milaboratory.

the class CloneAssemblerRunnerTest method runFullPipeline.

private static CloneSet runFullPipeline(String... fastqFiles) throws IOException, InterruptedException {
    // building alignments
    VDJCAlignerParameters alignerParameters = VDJCParametersPresets.getByName("default");
    VDJCAligner aligner = fastqFiles.length == 1 ? new VDJCAlignerS(alignerParameters) : new VDJCAlignerWithMerge(alignerParameters);
    for (VDJCGene gene : VDJCLibraryRegistry.getDefault().getLibrary("mi", "hs").getGenes(Chains.IGH)) if (alignerParameters.containsRequiredFeature(gene))
        aligner.addGene(gene);
    SequenceReader reader;
    if (fastqFiles.length == 1)
        reader = new SingleFastqReader(CloneAssemblerRunnerTest.class.getClassLoader().getResourceAsStream(fastqFiles[0]), true);
    else
        reader = new PairedFastqReader(CloneAssemblerRunnerTest.class.getClassLoader().getResourceAsStream(fastqFiles[0]), CloneAssemblerRunnerTest.class.getClassLoader().getResourceAsStream(fastqFiles[1]), true);
    // write alignments to byte array
    ByteArrayOutputStream alignmentsSerialized = new ByteArrayOutputStream();
    try (VDJCAlignmentsWriter writer = new VDJCAlignmentsWriter(alignmentsSerialized)) {
        writer.header(aligner);
        for (Object read : CUtils.it(reader)) {
            VDJCAlignmentResult result = (VDJCAlignmentResult) aligner.process((SequenceRead) read);
            if (result.alignment != null)
                writer.write(result.alignment);
        }
    }
    AlignmentsProvider alignmentsProvider = AlignmentsProvider.Util.createProvider(alignmentsSerialized.toByteArray(), VDJCLibraryRegistry.getDefault());
    LinearGapAlignmentScoring<NucleotideSequence> scoring = new LinearGapAlignmentScoring<>(NucleotideSequence.ALPHABET, 5, -9, -12);
    CloneFactoryParameters factoryParameters = new CloneFactoryParameters(new VJCClonalAlignerParameters(0.8f, scoring, 5), new VJCClonalAlignerParameters(0.8f, scoring, 5), null, new DAlignerParameters(GeneFeature.DRegion, 0.85f, 30.0f, 3, scoring));
    CloneAssemblerParameters assemblerParameters = new CloneAssemblerParameters(new GeneFeature[] { GeneFeature.CDR3 }, 12, QualityAggregationType.Average, new CloneClusteringParameters(2, 1, TreeSearchParameters.ONE_MISMATCH, new RelativeConcentrationFilter(1.0E-6)), factoryParameters, true, true, false, 0.4, true, (byte) 20, .8, "2 of 6", (byte) 15);
    System.out.println(GlobalObjectMappers.toOneLine(assemblerParameters));
    CloneAssemblerRunner assemblerRunner = new CloneAssemblerRunner(alignmentsProvider, new CloneAssembler(assemblerParameters, true, aligner.getUsedGenes(), alignerParameters), 2);
    SmartProgressReporter.startProgressReport(assemblerRunner);
    assemblerRunner.run();
    CloneSet cloneSet = assemblerRunner.getCloneSet(null);
    ByteArrayOutputStream bos = new ByteArrayOutputStream();
    CloneSetIO.write(cloneSet, bos);
    CloneSet cloneSetDeserialized = CloneSetIO.readClns(new ByteArrayInputStream(bos.toByteArray()));
    assertCSEquals(cloneSet, cloneSetDeserialized);
    OutputPortCloseable<ReadToCloneMapping> rrr = assemblerRunner.assembler.getAssembledReadsPort();
    ReadToCloneMapping take;
    while ((take = rrr.take()) != null) System.out.println(take);
    return cloneSet;
}