Examples with VDJCAlignments com.milaboratory.mixcr.basictypes.VDJCAlignments used on opensource projects

Example 11 with VDJCAlignments

use of com.milaboratory.mixcr.basictypes.VDJCAlignments in project mixcr by milaboratory.

the class VDJCAlignerS method processPartial.

private VDJCAlignmentResult<SingleRead> processPartial(SingleRead input) {
    Target[] targets = parameters.getReadsLayout().createTargets(input);
    KVJResultsForSingle[] results = new KVJResultsForSingle[targets.length];
    for (int i = 0; i < results.length; i++) {
        results[i] = align(targets[i]);
        results[i].performChainFilteringIfNeeded();
    }
    KVJResultsForSingle topResult = null;
    for (KVJResultsForSingle result : results) {
        if (!result.hasNoKVNorKJHits())
            result.alignDC();
        if (topResult == null || topResult.sumScore() < result.sumScore())
            topResult = result;
    }
    if (topResult.hasNoKVNorKJHits()) {
        onFailedAlignment(input, VDJCAlignmentFailCause.NoHits);
        return new VDJCAlignmentResult<>(input);
    }
    // Checking minimal sum score
    if (topResult.sumScore() < parameters.getMinSumScore()) {
        onFailedAlignment(input, VDJCAlignmentFailCause.LowTotalScore);
        return new VDJCAlignmentResult<>(input);
    }
    // Filtering hits basing on minSumScore
    topResult.calculateHits(parameters.getMinSumScore(), parameters.getMaxHits());
    VDJCAlignments alignment = topResult.toVDJCAlignments(input.getId(), input);
    // Final check
    if (!parameters.getAllowNoCDR3PartAlignments()) {
        // CDR3 Begin / End
        if (!alignment.getPartitionedTarget(0).getPartitioning().isAvailable(reqPointL) && !alignment.getPartitionedTarget(0).getPartitioning().isAvailable(reqPointR)) {
            onFailedAlignment(input, VDJCAlignmentFailCause.NoCDR3Parts);
            return new VDJCAlignmentResult<>(input);
        }
    }
    // Read successfully aligned
    onSuccessfulAlignment(input, alignment);
    return new VDJCAlignmentResult<>(input, alignment);
}

Example 12 with VDJCAlignments

use of com.milaboratory.mixcr.basictypes.VDJCAlignments in project mixcr by milaboratory.

the class FieldExtractors method getFields.

public static synchronized Field[] getFields() {
    if (descriptors == null) {
        List<Field> descriptorsList = new ArrayList<>();
        // Number of targets
        descriptorsList.add(new PL_O("-targets", "Export number of targets", "Number of targets", "numberOfTargets") {

            @Override
            protected String extract(VDJCObject object) {
                return Integer.toString(object.numberOfTargets());
            }
        });
        // Best hits
        for (final GeneType type : GeneType.values()) {
            char l = type.getLetter();
            descriptorsList.add(new PL_O("-" + Character.toLowerCase(l) + "Hit", "Export best " + l + " hit", "Best " + l + " hit", "best" + l + "Hit") {

                @Override
                protected String extract(VDJCObject object) {
                    VDJCHit bestHit = object.getBestHit(type);
                    if (bestHit == null)
                        return NULL;
                    return bestHit.getGene().getName();
                }
            });
        }
        // Best gene
        for (final GeneType type : GeneType.values()) {
            char l = type.getLetter();
            descriptorsList.add(new PL_O("-" + Character.toLowerCase(l) + "Gene", "Export best " + l + " hit gene name (e.g. TRBV12-3 for TRBV12-3*00)", "Best " + l + " gene", "best" + l + "Gene") {

                @Override
                protected String extract(VDJCObject object) {
                    VDJCHit bestHit = object.getBestHit(type);
                    if (bestHit == null)
                        return NULL;
                    return bestHit.getGene().getGeneName();
                }
            });
        }
        // Best family
        for (final GeneType type : GeneType.values()) {
            char l = type.getLetter();
            descriptorsList.add(new PL_O("-" + Character.toLowerCase(l) + "Family", "Export best " + l + " hit family name (e.g. TRBV12 for TRBV12-3*00)", "Best " + l + " family", "best" + l + "Family") {

                @Override
                protected String extract(VDJCObject object) {
                    VDJCHit bestHit = object.getBestHit(type);
                    if (bestHit == null)
                        return NULL;
                    return bestHit.getGene().getFamilyName();
                }
            });
        }
        // Best hit score
        for (final GeneType type : GeneType.values()) {
            char l = type.getLetter();
            descriptorsList.add(new PL_O("-" + Character.toLowerCase(l) + "HitScore", "Export score for best " + l + " hit", "Best " + l + " hit score", "best" + l + "HitScore") {

                @Override
                protected String extract(VDJCObject object) {
                    VDJCHit bestHit = object.getBestHit(type);
                    if (bestHit == null)
                        return NULL;
                    return String.valueOf(bestHit.getScore());
                }
            });
        }
        // All hits
        for (final GeneType type : GeneType.values()) {
            char l = type.getLetter();
            descriptorsList.add(new PL_O("-" + Character.toLowerCase(l) + "HitsWithScore", "Export all " + l + " hits with score", "All " + l + " hits", "all" + l + "HitsWithScore") {

                @Override
                protected String extract(VDJCObject object) {
                    VDJCHit[] hits = object.getHits(type);
                    if (hits.length == 0)
                        return "";
                    StringBuilder sb = new StringBuilder();
                    for (int i = 0; ; i++) {
                        sb.append(hits[i].getGene().getName()).append("(").append(SCORE_FORMAT.format(hits[i].getScore())).append(")");
                        if (i == hits.length - 1)
                            break;
                        sb.append(",");
                    }
                    return sb.toString();
                }
            });
        }
        // All hits without score
        for (final GeneType type : GeneType.values()) {
            char l = type.getLetter();
            descriptorsList.add(new PL_O("-" + Character.toLowerCase(l) + "Hits", "Export all " + l + " hits", "All " + l + " Hits", "all" + l + "Hits") {

                @Override
                protected String extract(VDJCObject object) {
                    VDJCHit[] hits = object.getHits(type);
                    if (hits.length == 0)
                        return "";
                    StringBuilder sb = new StringBuilder();
                    for (int i = 0; ; i++) {
                        sb.append(hits[i].getGene().getName());
                        if (i == hits.length - 1)
                            break;
                        sb.append(",");
                    }
                    return sb.toString();
                }
            });
        }
        // All gene names
        for (final GeneType type : GeneType.values()) {
            char l = type.getLetter();
            descriptorsList.add(new StringExtractor("-" + Character.toLowerCase(l) + "Genes", "Export all " + l + " gene names (e.g. TRBV12-3 for TRBV12-3*00)", "All " + l + " genes", "all" + l + "Genes", type) {

                @Override
                String extractStringForHit(VDJCHit hit) {
                    return hit.getGene().getGeneName();
                }
            });
        }
        // All families
        for (final GeneType type : GeneType.values()) {
            char l = type.getLetter();
            descriptorsList.add(new StringExtractor("-" + Character.toLowerCase(l) + "Families", "Export all " + l + " gene family anmes (e.g. TRBV12 for TRBV12-3*00)", "All " + l + " families", "all" + l + "Families", type) {

                @Override
                String extractStringForHit(VDJCHit hit) {
                    return hit.getGene().getFamilyName();
                }
            });
        }
        // Best alignment
        for (final GeneType type : GeneType.values()) {
            char l = type.getLetter();
            descriptorsList.add(new PL_O("-" + Character.toLowerCase(l) + "Alignment", "Export best " + l + " alignment", "Best " + l + " alignment", "best" + l + "Alignment") {

                @Override
                protected String extract(VDJCObject object) {
                    VDJCHit bestHit = object.getBestHit(type);
                    if (bestHit == null)
                        return NULL;
                    StringBuilder sb = new StringBuilder();
                    for (int i = 0; ; i++) {
                        Alignment<NucleotideSequence> alignment = bestHit.getAlignment(i);
                        if (alignment == null)
                            sb.append(NULL);
                        else
                            sb.append(alignment.toCompactString());
                        if (i == object.numberOfTargets() - 1)
                            break;
                        sb.append(",");
                    }
                    return sb.toString();
                }
            });
        }
        // All alignments
        for (final GeneType type : GeneType.values()) {
            char l = type.getLetter();
            descriptorsList.add(new PL_O("-" + Character.toLowerCase(l) + "Alignments", "Export all " + l + " alignments", "All " + l + " alignments", "all" + l + "Alignments") {

                @Override
                protected String extract(VDJCObject object) {
                    VDJCHit[] hits = object.getHits(type);
                    if (hits.length == 0)
                        return "";
                    StringBuilder sb = new StringBuilder();
                    for (int j = 0; ; ++j) {
                        for (int i = 0; ; i++) {
                            Alignment<NucleotideSequence> alignment = hits[j].getAlignment(i);
                            if (alignment == null)
                                sb.append(NULL);
                            else
                                sb.append(alignment.toCompactString());
                            if (i == object.numberOfTargets() - 1)
                                break;
                            sb.append(',');
                        }
                        if (j == hits.length - 1)
                            break;
                        sb.append(';');
                    }
                    return sb.toString();
                }
            });
        }
        descriptorsList.add(new FeatureExtractors.NSeqExtractor("-nFeature", "Export nucleotide sequence of specified gene feature", "N. Seq. ", "nSeq") {

            @Override
            public String convert(NSequenceWithQuality seq) {
                return seq.getSequence().toString();
            }
        });
        descriptorsList.add(new FeatureExtractors.NSeqExtractor("-qFeature", "Export quality string of specified gene feature", "Qual. ", "qual") {

            @Override
            public String convert(NSequenceWithQuality seq) {
                return seq.getQuality().toString();
            }
        });
        descriptorsList.add(new FeatureExtractors.WithHeader("-aaFeature", "Export amino acid sequence of specified gene feature", 1, new String[] { "AA. Seq. " }, new String[] { "aaSeq" }) {

            @Override
            protected String extractValue(VDJCObject object, GeneFeature[] parameters) {
                GeneFeature geneFeature = parameters[parameters.length - 1];
                NSequenceWithQuality feature = object.getFeature(geneFeature);
                if (feature == null)
                    return NULL;
                int targetId = object.getTargetContainingFeature(geneFeature);
                TranslationParameters tr = targetId == -1 ? TranslationParameters.FromLeftWithIncompleteCodon : object.getPartitionedTarget(targetId).getPartitioning().getTranslationParameters(geneFeature);
                if (tr == null)
                    return NULL;
                return AminoAcidSequence.translate(feature.getSequence(), tr).toString();
            }
        });
        // descriptorsList.add(new FeatureExtractorDescriptor("-aaFeatureFromLeft", "Export amino acid sequence of " +
        // "specified gene feature starting from the leftmost nucleotide (differs from -aaFeature only for " +
        // "sequences which length are not multiple of 3)", "AA. Seq.", "aaSeq") {
        // @Override
        // public String convert(NSequenceWithQuality seq) {
        // return AminoAcidSequence.translate(seq.getSequence(), FromLeftWithoutIncompleteCodon).toString();
        // }
        // });
        // 
        // descriptorsList.add(new FeatureExtractorDescriptor("-aaFeatureFromRight", "Export amino acid sequence of " +
        // "specified gene feature starting from the rightmost nucleotide (differs from -aaFeature only for " +
        // "sequences which length are not multiple of 3)", "AA. Seq.", "aaSeq") {
        // @Override
        // public String convert(NSequenceWithQuality seq) {
        // return AminoAcidSequence.translate(seq.getSequence(), FromRightWithoutIncompleteCodon).toString();
        // }
        // });
        descriptorsList.add(new FeatureExtractors.NSeqExtractor("-minFeatureQuality", "Export minimal quality of specified gene feature", "Min. qual. ", "minQual") {

            @Override
            public String convert(NSequenceWithQuality seq) {
                return "" + seq.getQuality().minValue();
            }
        });
        descriptorsList.add(new FeatureExtractors.NSeqExtractor("-avrgFeatureQuality", "Export average quality of specified gene feature", "Mean. qual. ", "meanQual") {

            @Override
            public String convert(NSequenceWithQuality seq) {
                return "" + seq.getQuality().meanValue();
            }
        });
        descriptorsList.add(new FeatureExtractors.NSeqExtractor("-lengthOf", "Exports length of specified gene feature.", "Length of ", "lengthOf") {

            @Override
            public String convert(NSequenceWithQuality seq) {
                return "" + seq.size();
            }
        });
        descriptorsList.add(new FeatureExtractors.MutationsExtractor("-nMutations", "Extract nucleotide mutations for specific gene feature; relative to germline sequence.", 1, new String[] { "N. Mutations in " }, new String[] { "nMutations" }) {

            @Override
            String convert(Mutations<NucleotideSequence> mutations, NucleotideSequence seq1, NucleotideSequence seq2, TranslationParameters tr) {
                return mutations.encode(",");
            }
        });
        descriptorsList.add(new FeatureExtractors.MutationsExtractor("-nMutationsRelative", "Extract nucleotide mutations for specific gene feature relative to another feature.", 2, new String[] { "N. Mutations in ", " relative to " }, new String[] { "nMutationsIn", "Relative" }) {

            @Override
            String convert(Mutations<NucleotideSequence> mutations, NucleotideSequence seq1, NucleotideSequence seq2, TranslationParameters tr) {
                return mutations.encode(",");
            }
        });
        final class AAMutations extends FeatureExtractors.MutationsExtractor {

            AAMutations(String command, String description, int nArgs, String[] hPrefix, String[] sPrefix) {
                super(command, description, nArgs, hPrefix, sPrefix);
            }

            @Override
            String convert(Mutations<NucleotideSequence> mutations, NucleotideSequence seq1, NucleotideSequence seq2, TranslationParameters tr) {
                if (tr == null)
                    return "-";
                Mutations<AminoAcidSequence> aaMuts = MutationsUtil.nt2aa(seq1, mutations, tr);
                if (aaMuts == null)
                    return "-";
                return aaMuts.encode(",");
            }
        }
        descriptorsList.add(new AAMutations("-aaMutations", "Extract amino acid mutations for specific gene feature", 1, new String[] { "AA. Mutations in " }, new String[] { "aaMutations" }));
        descriptorsList.add(new AAMutations("-aaMutationsRelative", "Extract amino acid mutations for specific gene feature relative to another feature.", 2, new String[] { "AA. Mutations in ", " relative to " }, new String[] { "aaMutationsIn", "Relative" }));
        final class MutationsDetailed extends FeatureExtractors.MutationsExtractor {

            MutationsDetailed(String command, String description, int nArgs, String[] hPrefix, String[] sPrefix) {
                super(command, description, nArgs, hPrefix, sPrefix);
            }

            @Override
            String convert(Mutations<NucleotideSequence> mutations, NucleotideSequence seq1, NucleotideSequence seq2, TranslationParameters tr) {
                if (tr == null)
                    return "-";
                MutationsUtil.MutationNt2AADescriptor[] descriptors = MutationsUtil.nt2aaDetailed(seq1, mutations, tr, 10);
                if (descriptors == null)
                    return "-";
                StringBuilder sb = new StringBuilder();
                for (int i = 0; i < descriptors.length; i++) {
                    sb.append(descriptors[i]);
                    if (i == descriptors.length - 1)
                        break;
                    sb.append(",");
                }
                return sb.toString();
            }
        }
        String detailedMutationsFormat = "Format <nt_mutation>:<aa_mutation_individual>:<aa_mutation_cumulative>, where <aa_mutation_individual> is an expected amino acid " + "mutation given no other mutations have occurred, and <aa_mutation_cumulative> amino acid mutation is the observed amino acid " + "mutation combining effect from all other. WARNING: format may change in following versions.";
        descriptorsList.add(new MutationsDetailed("-mutationsDetailed", "Detailed list of nucleotide and corresponding amino acid mutations. " + detailedMutationsFormat, 1, new String[] { "Detailed mutations in " }, new String[] { "mutationsDetailedIn" }));
        descriptorsList.add(new MutationsDetailed("-mutationsDetailedRelative", "Detailed list of nucleotide and corresponding amino acid mutations written, positions relative to specified gene feature. " + detailedMutationsFormat, 2, new String[] { "Detailed mutations in ", " relative to " }, new String[] { "mutationsDetailedIn", "Relative" }));
        descriptorsList.add(new ExtractReferencePointPosition());
        descriptorsList.add(new ExtractDefaultReferencePointsPositions());
        descriptorsList.add(new PL_A("-readId", "Export id of read corresponding to alignment", "Read id", "readId") {

            @Override
            protected String extract(VDJCAlignments object) {
                return "" + object.getMinReadId();
            }

            @Override
            public FieldExtractor<VDJCAlignments> create(OutputMode outputMode, String[] args) {
                System.out.println("WARNING: -readId is deprecated. Use -readIds");
                return super.create(outputMode, args);
            }
        });
        descriptorsList.add(new PL_A("-readIds", "Export id of read corresponding to alignment", "Read id", "readId") {

            @Override
            protected String extract(VDJCAlignments object) {
                long[] readIds = object.getReadIds();
                StringBuilder sb = new StringBuilder();
                for (int i = 0; ; i++) {
                    sb.append(readIds[i]);
                    if (i == readIds.length - 1)
                        return sb.toString();
                    sb.append(",");
                }
            }
        });
        descriptorsList.add(new ExtractSequence(VDJCAlignments.class, "-sequence", "Export aligned sequence (initial read), or 2 sequences in case of paired-end reads", "Read(s) sequence", "readSequence"));
        descriptorsList.add(new ExtractSequenceQuality(VDJCAlignments.class, "-quality", "Export initial read quality, or 2 qualities in case of paired-end reads", "Read(s) sequence qualities", "readQuality"));
        descriptorsList.add(new PL_C("-cloneId", "Unique clone identifier", "Clone ID", "cloneId") {

            @Override
            protected String extract(Clone object) {
                return "" + object.getId();
            }
        });
        descriptorsList.add(new PL_C("-count", "Export clone count", "Clone count", "cloneCount") {

            @Override
            protected String extract(Clone object) {
                return "" + object.getCount();
            }
        });
        descriptorsList.add(new PL_C("-fraction", "Export clone fraction", "Clone fraction", "cloneFraction") {

            @Override
            protected String extract(Clone object) {
                return "" + object.getFraction();
            }
        });
        descriptorsList.add(new ExtractSequence(Clone.class, "-sequence", "Export aligned sequence (initial read), or 2 sequences in case of paired-end reads", "Clonal sequence(s)", "clonalSequence"));
        descriptorsList.add(new ExtractSequenceQuality(Clone.class, "-quality", "Export initial read quality, or 2 qualities in case of paired-end reads", "Clonal sequence quality(s)", "clonalSequenceQuality"));
        descriptorsList.add(new PL_A("-descrR1", "Export description line from initial .fasta or .fastq file " + "of the first read (only available if --save-description was used in align command)", "Description R1", "descrR1") {

            @Override
            protected String extract(VDJCAlignments object) {
                List<SequenceRead> reads = object.getOriginalReads();
                if (reads == null)
                    throw new IllegalArgumentException("Error for option \'-descrR1\':\n" + "No description available for read: either re-run align action with -OsaveOriginalReads=true option " + "or don't use \'-descrR1\' in exportAlignments");
                return reads.get(0).getRead(0).getDescription();
            }

            @Override
            public FieldExtractor<VDJCAlignments> create(OutputMode outputMode, String[] args) {
                System.out.println("WARNING: -descrR1 is deprecated. Use -descrsR1");
                return super.create(outputMode, args);
            }
        });
        descriptorsList.add(new PL_A("-descrR2", "Export description line from initial .fasta or .fastq file " + "of the second read (only available if --save-description was used in align command)", "Description R2", "descrR2") {

            @Override
            protected String extract(VDJCAlignments object) {
                List<SequenceRead> reads = object.getOriginalReads();
                if (reads == null)
                    throw new IllegalArgumentException("Error for option \'-descrR1\':\n" + "No description available for read: either re-run align action with -OsaveOriginalReads=true option " + "or don't use \'-descrR1\' in exportAlignments");
                SequenceRead read = reads.get(0);
                if (read.numberOfReads() < 2)
                    throw new IllegalArgumentException("Error for option \'-descrR2\':\n" + "No description available for second read: your input data was single-end");
                return read.getRead(1).getDescription();
            }

            @Override
            public FieldExtractor<VDJCAlignments> create(OutputMode outputMode, String[] args) {
                System.out.println("WARNING: -descrR2 is deprecated. Use -descrsR2");
                return super.create(outputMode, args);
            }
        });
        descriptorsList.add(new PL_A("-descrsR1", "Export description lines from initial .fasta or .fastq file " + "of the first reads (only available if -OsaveOriginalReads=true was used in align command)", "Descriptions R1", "descrsR1") {

            @Override
            protected String extract(VDJCAlignments object) {
                List<SequenceRead> reads = object.getOriginalReads();
                if (reads == null)
                    throw new IllegalArgumentException("Error for option \'-descrR1\':\n" + "No description available for read: either re-run align action with -OsaveOriginalReads option " + "or don't use \'-descrR1\' in exportAlignments");
                StringBuilder sb = new StringBuilder();
                for (int i = 0; ; i++) {
                    sb.append(reads.get(i).getRead(0).getDescription());
                    if (i == reads.size() - 1)
                        return sb.toString();
                    sb.append(",");
                }
            }
        });
        descriptorsList.add(new PL_A("-descrsR2", "Export description lines from initial .fasta or .fastq file " + "of the second reads (only available if -OsaveOriginalReads=true was used in align command)", "Descriptions R2", "descrsR2") {

            @Override
            protected String extract(VDJCAlignments object) {
                List<SequenceRead> reads = object.getOriginalReads();
                if (reads == null)
                    throw new IllegalArgumentException("Error for option \'-descrR1\':\n" + "No description available for read: either re-run align action with -OsaveOriginalReads option " + "or don't use \'-descrR1\' in exportAlignments");
                StringBuilder sb = new StringBuilder();
                for (int i = 0; ; i++) {
                    SequenceRead read = reads.get(i);
                    if (read.numberOfReads() < 2)
                        throw new IllegalArgumentException("Error for option \'-descrsR2\':\n" + "No description available for second read: your input data was single-end");
                    sb.append(read.getRead(1).getDescription());
                    if (i == reads.size() - 1)
                        return sb.toString();
                    sb.append(",");
                }
            }
        });
        descriptorsList.add(new PL_A("-readHistory", "Export read history", "Read history", "readHistory") {

            @Override
            protected String extract(VDJCAlignments object) {
                try {
                    return GlobalObjectMappers.toOneLine(object.getHistory());
                } catch (JsonProcessingException ex) {
                    throw new RuntimeException(ex);
                }
            }
        });
        for (final GeneType type : GeneType.values()) {
            String c = Character.toLowerCase(type.getLetter()) + "IdentityPercents";
            descriptorsList.add(new PL_O("-" + c, type.getLetter() + " alignment identity percents", type.getLetter() + " alignment identity percents", c) {

                @Override
                protected String extract(VDJCObject object) {
                    VDJCHit[] hits = object.getHits(type);
                    if (hits == null)
                        return NULL;
                    StringBuilder sb = new StringBuilder();
                    sb.append("");
                    for (int i = 0; ; i++) {
                        sb.append(hits[i].getIdentity());
                        if (i == hits.length - 1)
                            return sb.toString();
                        sb.append(",");
                    }
                }
            });
        }
        for (final GeneType type : GeneType.values()) {
            String c = Character.toLowerCase(type.getLetter()) + "BestIdentityPercent";
            descriptorsList.add(new PL_O("-" + c, type.getLetter() + "best alignment identity percent", type.getLetter() + "best alignment identity percent", c) {

                @Override
                protected String extract(VDJCObject object) {
                    VDJCHit hit = object.getBestHit(type);
                    if (hit == null)
                        return NULL;
                    return Float.toString(hit.getIdentity());
                }
            });
        }
        descriptorsList.add(new PL_O("-chains", "Chains", "Chains", "Chains") {

            @Override
            protected String extract(VDJCObject object) {
                return object.commonChains().toString();
            }
        });
        descriptorsList.add(new PL_O("-topChains", "Top chains", "Top chains", "topChains") {

            @Override
            protected String extract(VDJCObject object) {
                return object.commonTopChains().toString();
            }
        });
        descriptors = descriptorsList.toArray(new Field[descriptorsList.size()]);
    }
    return descriptors;
}

Example 13 with VDJCAlignments

use of com.milaboratory.mixcr.basictypes.VDJCAlignments in project mixcr by milaboratory.

the class ActionExportCloneReads method go.

@Override
public void go(ActionHelper helper) throws Exception {
    try (ClnAReader clna = new ClnAReader(parameters.getInputFileName(), VDJCLibraryRegistry.createDefaultRegistry())) {
        VDJCAlignments firstAlignment = clna.readAllAlignments().take();
        if (firstAlignment == null)
            return;
        if (firstAlignment.getOriginalReads() == null)
            throw new ParameterException("Error: original reads were not saved in the .vdjca file: " + "re-run align with '-g' option.");
        int[] cid = parameters.getCloneIds();
        Supplier<IntStream> cloneIds;
        if (cid == null)
            cloneIds = () -> IntStream.range(0, clna.numberOfClones());
        else
            cloneIds = () -> IntStream.of(cid);
        long totalAlignments = cloneIds.get().mapToLong(clna::numberOfAlignmentsInClone).sum();
        AtomicLong alignmentsWritten = new AtomicLong();
        AtomicBoolean finished = new AtomicBoolean(false);
        SmartProgressReporter.startProgressReport("Writing reads", new CanReportProgress() {

            @Override
            public double getProgress() {
                return 1.0 * alignmentsWritten.get() / totalAlignments;
            }

            @Override
            public boolean isFinished() {
                return finished.get();
            }
        });
        boolean paired = firstAlignment.getOriginalReads().get(0).numberOfReads() == 2;
        boolean separate = parameters.doSeparate();
        SequenceWriter globalWriter = separate ? null : createWriter(paired, parameters.getOutputFileName());
        cloneIds.get().forEach(cloneId -> {
            try (SequenceWriter individualWriter = globalWriter == null ? createWriter(paired, cloneFile(parameters.getOutputFileName(), cloneId)) : null) {
                SequenceWriter actualWriter = globalWriter == null ? individualWriter : globalWriter;
                for (VDJCAlignments alignments : CUtils.it(clna.readAlignmentsOfClone(cloneId))) {
                    for (SequenceRead read : alignments.getOriginalReads()) actualWriter.write(read);
                    alignmentsWritten.incrementAndGet();
                }
            } catch (IOException e) {
                throw new RuntimeException(e);
            }
        });
    }
}

Example 14 with VDJCAlignments

use of com.milaboratory.mixcr.basictypes.VDJCAlignments in project mixcr by milaboratory.

the class ActionInfo method processAlignmentsFile.

public void processAlignmentsFile(String name) throws IOException {
    long size = Files.size(Paths.get(name));
    try (VDJCAlignmentsReader reader = new VDJCAlignmentsReader(name)) {
        long numberOfAlignedReads = 0;
        if (size > 30000000)
            SmartProgressReporter.startProgressReport("Processing " + name, reader, System.err);
        else
            System.err.println("Processing " + name + "...");
        for (VDJCAlignments alignments : CUtils.it(reader)) {
            numberOfAlignedReads++;
        }
        tableAdapter.row(name, reader.getNumberOfReads(), numberOfAlignedReads);
    }
}

Example 15 with VDJCAlignments

use of com.milaboratory.mixcr.basictypes.VDJCAlignments in project mixcr by milaboratory.

the class ActionMergeAlignments method go.

@Override
public void go(ActionHelper helper) throws Exception {
    try (MultiReader reader = new MultiReader(parameters.getInputFileNames());
        VDJCAlignmentsWriter writer = new VDJCAlignmentsWriter(parameters.getOutputFileName())) {
        reader.initNextReader();
        SmartProgressReporter.startProgressReport("Merging", reader);
        writer.header(reader.currentInnerReader.getParameters(), reader.currentInnerReader.getUsedGenes());
        for (VDJCAlignments record : CUtils.it(reader)) writer.write(record);
        writer.setNumberOfProcessedReads(reader.readIdOffset.get());
    }
}