Example 1 with Blast6Segment
use of cz1.ngs.model.Blast6Segment in project polyGembler by c-zhou.
the class Redundas method run.
@Override
public void run() {
// TODO Auto-generated method stub
Map<String, Sequence> sequence_map = Sequence.parseFastaFileAsMap(query_file);
String line;
String[] s;
Set<String> seq_rm = new HashSet<String>();
try {
BufferedReader br_blast6 = new BufferedReader(new FileReader(blast_out));
Set<Blast6Segment> buffer_b6 = new HashSet<Blast6Segment>();
line = br_blast6.readLine();
String qseqid;
while (line != null) {
s = line.split("\\s+");
qseqid = s[0];
buffer_b6.clear();
buffer_b6.add(Blast6Segment.blast6Segment(line));
while ((line = br_blast6.readLine()) != null && line.startsWith(qseqid)) buffer_b6.add(Blast6Segment.blast6Segment(line));
int sz = sequence_map.get(qseqid).seq_ln();
RangeSet<Integer> range_covered = TreeRangeSet.create();
for (Blast6Segment record : buffer_b6) {
if (!seq_rm.contains(record.sseqid()) && !record.qseqid().equals(record.sseqid()) && record.pident() >= min_ident && record.length() >= min_match && (record.qstart() <= max_overhang || record.qend() > sz - max_overhang))
range_covered.add(Range.closedOpen(record.qstart(), record.qend()).canonical(DiscreteDomain.integers()));
}
int unique_cvg = 0;
for (Range<Integer> range : range_covered.asRanges()) unique_cvg += range.upperEndpoint() - range.lowerEndpoint();
if ((double) unique_cvg / sz >= min_frac) {
seq_rm.add(qseqid);
myLogger.info("Redundant sequence " + qseqid + "\t" + sz + "\t" + unique_cvg + "\t" + (double) unique_cvg / sz);
}
}
br_blast6.close();
BufferedWriter bw_unique = Utils.getBufferedWriter(this.out_prefix + ".fa");
BufferedWriter bw_redundas = Utils.getBufferedWriter(this.out_prefix + "_redundas.fa");
for (Map.Entry<String, Sequence> entry : sequence_map.entrySet()) {
if (seq_rm.contains(entry.getKey()))
bw_redundas.write(entry.getValue().formatOutput());
else
bw_unique.write(entry.getValue().formatOutput());
}
bw_unique.close();
bw_redundas.close();
} catch (IOException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
Also used :
Blast6Segment(cz1.ngs.model.Blast6Segment)
Sequence(cz1.ngs.model.Sequence)
IOException(java.io.IOException)
BufferedWriter(java.io.BufferedWriter)
BufferedReader(java.io.BufferedReader)
FileReader(java.io.FileReader)
HashMap(java.util.HashMap)
Map(java.util.Map)
HashSet(java.util.HashSet)
Example 2 with Blast6Segment
use of cz1.ngs.model.Blast6Segment in project polyGembler by c-zhou.
the class Anchor method run.
// private static DijkstraShortestPath<Integer, DefaultWeightedEdge> dijkstra_paths;
@Override
public void run() {
// TODO Auto-generated method stub
long tic = System.nanoTime();
sub_seqs = Sequence.parseFastaFileAsMap(subject_file);
qry_seqs = Sequence.parseFastaFileWithRevCmpAsMap(query_file);
// this.makeAssemblyGraph(query_file);
this.makeAssemblyGraph(query_file);
bfs = new BFSShortestPath<Integer, DefaultWeightedEdge>(assembly_graph);
// dijkstra_paths = new DijkstraShortestPath<Integer, DefaultWeightedEdge>(assembly_graph);
// find 'N/n's in subject/reference sequences
// which could have impact on parsing the blast records
final Map<String, TreeRangeSet<Integer>> sub_gaps = new HashMap<String, TreeRangeSet<Integer>>();
final Map<String, List<Blast6Segment>> anchored_records = new HashMap<String, List<Blast6Segment>>();
for (Map.Entry<String, Sequence> entry : sub_seqs.entrySet()) {
String seq_sn = entry.getKey();
String seq_str = entry.getValue().seq_str();
final TreeRangeSet<Integer> tmp_rangeSet = TreeRangeSet.create();
for (int j = 0; j < seq_str.length(); j++) {
if (seq_str.charAt(j) == 'N' || seq_str.charAt(j) == 'n')
// blast record is 1-based closed coordination
tmp_rangeSet.add(Range.closed(j + 1, j + 1).canonical(DiscreteDomain.integers()));
}
int seq_ln = seq_str.length();
final TreeRangeSet<Integer> range_set = TreeRangeSet.create();
for (Range<Integer> range : tmp_rangeSet.asRanges()) {
int lowerend = range.hasLowerBound() ? Math.max(0, range.lowerEndpoint() - gap_buff) : 0;
int upperend = range.hasUpperBound() ? Math.min(seq_ln, range.upperEndpoint() + gap_buff - 1) : seq_ln;
range_set.add(Range.closed(lowerend, upperend).canonical(DiscreteDomain.integers()));
}
sub_gaps.put(seq_sn, range_set);
// initialise an array list for each reference chromosome
anchored_records.put(seq_sn, new ArrayList<Blast6Segment>());
}
// parse blast records
// blast records buffer
final List<Blast6Segment> buff = new ArrayList<Blast6Segment>();
// selected blast records
final List<Blast6Segment> sel_recs = new ArrayList<Blast6Segment>();
// temp list
final List<Blast6Segment> tmp_records = new ArrayList<Blast6Segment>();
try {
BufferedReader br_blast = Utils.getBufferedReader(blast_out);
Blast6Segment tmp_record = Blast6Segment.blast6Segment(br_blast.readLine());
Blast6Segment primary_record, secondary_record;
String qry;
double qry_ln, aln_frac;
while (tmp_record != null) {
qry = tmp_record.qseqid();
qry_ln = qry_seqs.get(qry).seq_ln();
buff.clear();
buff.add(tmp_record);
while ((tmp_record = Blast6Segment.blast6Segment(br_blast.readLine())) != null && tmp_record.qseqid().equals(qry)) {
// filter by identity
if (tmp_record.pident() >= this.min_ident)
buff.add(tmp_record);
}
sel_recs.clear();
// merge collinear records
for (String sub : sub_seqs.keySet()) {
// get all records for subject/reference sequence sub_seq
tmp_records.clear();
for (Blast6Segment record : buff) if (record.sseqid().equals(sub))
tmp_records.add(record);
if (tmp_records.isEmpty())
continue;
// find alignment segments that can be deleted
// those that are subsets of larger alignment segments
// (Sstart, (sstart, send), Send) and (Qstart, (qstart, qend), Qend)
Collections.sort(tmp_records, new Blast6Segment.SegmentSizeComparator());
final Set<int[][]> ranges = new HashSet<int[][]>();
outerloop: for (int i = 0; i < tmp_records.size(); i++) {
primary_record = tmp_records.get(i);
int[][] range = new int[2][2];
if (primary_record.sstart() < primary_record.send()) {
range[0][0] = primary_record.sstart();
range[0][1] = primary_record.send();
} else {
range[0][0] = primary_record.send();
range[0][1] = primary_record.sstart();
}
if (primary_record.qstart() < primary_record.qend()) {
range[1][0] = primary_record.qstart();
range[1][1] = primary_record.qend();
} else {
range[1][0] = primary_record.qend();
range[1][1] = primary_record.qstart();
}
for (int[][] r : ranges) {
if (r[0][0] <= range[0][0] && r[0][1] >= range[0][1] && r[1][0] <= range[1][0] && r[1][1] >= range[1][1]) {
tmp_records.remove(i--);
continue outerloop;
}
}
ranges.add(range);
}
// TODO rewrite this part
/**
* // find collinear alignment segments that can be merged
* Collections.sort(tmp_records, new BlastRecord.SInterceptComparator());
*
* collinear_merged.clear();
* final List<Blast6Record> temp = new ArrayList<Blast6Record>();
* for(int i=0; i<tmp_records.size(); ) {
* Blast6Record record = tmp_records.get(i);
* double max_shift;
* temp.clear();
* temp.add(record);
*
* // find collinear alignment segments
* outerloop:
* while( (++i)<tmp_records.size() ) {
* record = tmp_records.get(i);
* // check if is collinear with other alignment segments
* for(Blast6Record r : temp) {
* max_shift = collinear_shift*
* Math.min(r.length(), record.length());
* if(BlastRecord.sdistance(r, record)<=max_shift &&
* BlastRecord.qdistance(r, record)<=max_shift &&
* BlastRecord.pdistance(r, record)<=max_shift) {
* temp.add(record);
* continue outerloop;
* }
* }
* break;
* }
*
* // merge collinear alignment segments
* int qstart = Integer.MAX_VALUE;
* int qend = Integer.MIN_VALUE;
* int sstart = Integer.MAX_VALUE;
* int send = Integer.MIN_VALUE;
* double pident = 0;
* int length = 0;
*
* // qstart is always smaller than qend
* for(int j=0; j<temp.size(); j++) {
* record = temp.get(j);
* if(record.qstart()<qstart) {
* qstart = record.qstart();
* sstart = record.sstart();
* }
* if(record.qend()>qend) {
* qend = record.qend();
* send = record.send();
* }
* if(record.pident()>pident)
* pident = record.pident();
* length += record.length();
* }
*
* collinear_merged.add(new Blast6Record(qry,sub,pident,length,-1,-1,qstart,qend,sstart,send,-1,-1));
* }
*/
// find collinear alignment segments that can be merged
// more accurate but slower
Collections.sort(tmp_records, new AlignmentSegment.SubjectCoordinationComparator());
Blast6Segment record;
for (int i = 0; i < tmp_records.size(); i++) {
primary_record = tmp_records.get(i);
for (int j = i + 1; j < tmp_records.size(); j++) {
secondary_record = tmp_records.get(j);
double max_shift = collinear_shift * Math.min(primary_record.length(), secondary_record.length());
if ((record = Blast6Segment.collinear(primary_record, secondary_record, max_shift)) != null) {
tmp_records.set(i, record);
tmp_records.remove(j);
--i;
break;
}
}
}
// process blast records that clipped by gaps
// (sstart, send)---(start2, send2)
// (sstart ... --- ... send2)
TreeRangeSet<Integer> sub_gap = sub_gaps.get(sub);
Collections.sort(tmp_records, new AlignmentSegment.SubjectCoordinationComparator());
for (int i = 0; i < tmp_records.size(); i++) {
primary_record = tmp_records.get(i);
if (sub_gap.contains(primary_record.true_send())) {
secondary_record = null;
int sec_j = -1;
for (int j = i + 1; j < tmp_records.size(); j++) {
if (tmp_records.get(j).true_sstart() >= primary_record.true_send()) {
secondary_record = tmp_records.get(j);
sec_j = j;
break;
}
}
if (secondary_record == null || AlignmentSegment.reverse(primary_record, secondary_record)) {
// reverse alignment segments
continue;
}
if (sub_gap.contains(secondary_record.true_sstart()) && sub_gap.rangeContaining(primary_record.true_send()).equals(sub_gap.rangeContaining(secondary_record.true_sstart()))) {
// clipping
// merge two alignment segments
double pident = Math.max(primary_record.pident(), secondary_record.pident());
int qstart = Math.min(primary_record.true_qstart(), secondary_record.true_qstart());
int qend = Math.max(primary_record.true_qend(), secondary_record.true_qend());
int sstart = Math.min(primary_record.true_sstart(), secondary_record.true_sstart());
int send = Math.max(primary_record.true_send(), secondary_record.true_send());
int length = qend - qstart + 1;
// replace primary record with merged record
// delete secondary record
Blast6Segment merged_record = primary_record.forward() ? new Blast6Segment(qry, sub, pident, length, -1, -1, qstart, qend, sstart, send, -1, -1) : new Blast6Segment(qry, sub, pident, length, -1, -1, qstart, qend, send, sstart, -1, -1);
tmp_records.set(i, merged_record);
tmp_records.remove(sec_j);
// the merged records need to be processed
--i;
}
}
}
// add to sel_recs
sel_recs.addAll(tmp_records);
}
// filter by alignment fraction
buff.clear();
buff.addAll(sel_recs);
sel_recs.clear();
for (Blast6Segment record : buff) {
if (record.length() / qry_ln >= this.min_frac)
sel_recs.add(record);
}
if (sel_recs.isEmpty()) {
// continue
continue;
}
// filter blast records
Collections.sort(sel_recs, new Blast6Segment.MatchIndentityComparator());
// process primary alignment
primary_record = sel_recs.get(0);
anchored_records.get(primary_record.sseqid()).add(primary_record);
aln_frac = primary_record.length() / qry_ln;
// and process
for (int i = 1; i < sel_recs.size(); i++) {
secondary_record = sel_recs.get(i);
if (secondary_record.pident() + this.diff_ident < primary_record.pident() || secondary_record.length() / qry_ln + this.diff_frac < aln_frac) {
break;
} else {
anchored_records.get(secondary_record.sseqid()).add(secondary_record);
}
}
}
br_blast.close();
for (Map.Entry<String, List<Blast6Segment>> entry : anchored_records.entrySet()) {
System.out.println(entry.getKey() + ": " + entry.getValue().size());
}
final BufferedWriter bw_map = Utils.getBufferedWriter(out_prefix + ".map");
final BufferedWriter bw_fa = Utils.getBufferedWriter(out_prefix + ".fa");
final Set<String> anchored_seqs = new HashSet<String>();
final List<String> sub_list = Sequence.parseSeqList(subject_file);
for (String sub_sn : sub_list) {
blast6_records = anchored_records.get(sub_sn);
int nV = blast6_records.size(), count = 0;
// sort blast records
Collections.sort(blast6_records, new Blast6Segment.SubjectCoordinationComparator());
// consensus
int posUpto = 0, send_clip = 0;
int sstart, send, qstart, qend, qlen, tmp_int, qstart_clip, qend_clip, gap_size;
// distance to last 'N', start from next position to find longest common suffix-prefix
int prev_n = Integer.MAX_VALUE;
int mol_len = 0;
String qseq;
int nS, nQ;
// first step: construct super scaffold
// will form a tree graph indicating the path through the contigs
// convert contig names to integer indices
// one contig could end up with multiple indices due to repeats
Map<Integer, String> ss_coordinate = new HashMap<Integer, String>();
int index = 0;
for (Blast6Segment record : blast6_records) ss_coordinate.put(index++, record.qseqid() + (record.forward() ? "" : "'"));
StringBuilder seq_str = new StringBuilder();
for (int v = 0; v < nV - 1; v++) {
if (++count % 10000 == 0)
myLogger.info(sub_sn + " " + count + "/" + nV + " done.");
Blast6Segment record = blast6_records.get(v);
qlen = qry_seqs.get(record.qseqid()).seq_ln();
sstart = record.sstart();
send = record.send();
qstart = record.qstart();
qend = record.qend();
if (sstart > send) {
// make sure sstart<send
tmp_int = sstart;
sstart = send;
send = tmp_int;
tmp_int = qstart;
qstart = qend;
qend = tmp_int;
}
if (qstart > qend) {
qstart_clip = qlen - qstart;
qend_clip = qend - 1;
qseq = Sequence.revCompSeq(qry_seqs.get(record.qseqid()).seq_str());
} else {
qstart_clip = qstart - 1;
qend_clip = qlen - qend;
qseq = qry_seqs.get(record.qseqid()).seq_str();
}
if (send < posUpto || sstart < posUpto && qstart_clip > max_clip) {
// TODO process
continue;
}
// find longest suffix-prefix
nS = seq_str.length();
nQ = qseq.length();
int nO = Math.min(prev_n, Math.min(nS, nQ));
outerloop: for (; nO >= min_overlap; nO--) {
int nS_i = nS - nO;
for (int i = 0; i < nO; i++) {
if (seq_str.charAt(nS_i + i) != qseq.charAt(i))
continue outerloop;
}
break outerloop;
}
if (nO < min_overlap) {
// otherwise will insert a large GAP max(pseduo_distance, max_gap)
if (posUpto > 0) {
if (sstart <= posUpto) {
// if too much overlap, then treat it as a contradiction
if (posUpto - sstart > min_overlap) {
// discard
continue;
} else {
// insert a min_gap
gap_size = min_gap;
}
} else {
// estimate gap size
gap_size = (sstart - posUpto) - (send_clip + qstart_clip);
if (gap_size < max_gap)
gap_size = max_gap;
}
bw_map.write("GAP\t" + gap_size + "\t0\t" + gap_size + "\t+\t" + sub_sn + "\t" + mol_len + "\t" + (mol_len + gap_size) + "\n");
seq_str.append(Sequence.polyN(gap_size));
mol_len += gap_size;
}
bw_map.write(record.qseqid() + "\t" + qlen + "\t");
if (qstart > qend) {
// reverse
bw_map.write("0\t" + qlen + "\t-\t");
} else {
// forward
bw_map.write("0\t" + qlen + "\t+\t");
}
seq_str.append(qseq);
bw_map.write(sub_sn + "\t" + mol_len + "\t" + (mol_len + qlen) + "\n");
mol_len += qlen;
prev_n = qlen;
anchored_seqs.add(record.qseqid());
} else {
// overlap found
// will not insert gap
// ====================
// /-------\
// /----\
// calculate overlaps
// process overlap
qstart = nO;
if (qstart == qlen)
continue;
bw_map.write(record.qseqid() + "\t" + (qlen - qstart) + "\t");
if (qstart > qend) {
// reverse
bw_map.write(0 + "\t" + (qlen - qstart) + "\t-\t");
} else {
// forward
bw_map.write(qstart + "\t" + qlen + "\t+\t");
}
bw_map.write(sub_sn + "\t" + mol_len + "\t" + (mol_len + qlen - qstart) + "\n");
mol_len += qlen - qstart;
prev_n += qlen - qstart;
seq_str.append(qseq.substring(qstart));
anchored_seqs.add(record.qseqid());
}
posUpto = send;
send_clip = qend_clip;
}
if (seq_str.length() > 0)
bw_fa.write(Sequence.formatOutput(sub_sn, seq_str.toString()));
}
bw_fa.close();
bw_map.close();
final BufferedWriter bw_ufa = Utils.getBufferedWriter(out_prefix + "_unplaced.fa");
for (String seq : qry_seqs.keySet()) if (!anchored_seqs.contains(seq))
bw_ufa.write(qry_seqs.get(seq).formatOutput());
bw_ufa.close();
} catch (IOException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
Also used :
TreeRangeSet(com.google.common.collect.TreeRangeSet)
HashMap(java.util.HashMap)
Blast6Segment(cz1.ngs.model.Blast6Segment)
ArrayList(java.util.ArrayList)
BufferedWriter(java.io.BufferedWriter)
ArrayList(java.util.ArrayList)
List(java.util.List)
HashSet(java.util.HashSet)
Sequence(cz1.ngs.model.Sequence)
IOException(java.io.IOException)
DefaultWeightedEdge(org.jgrapht.graph.DefaultWeightedEdge)
AlignmentSegment(cz1.ngs.model.AlignmentSegment)
BufferedReader(java.io.BufferedReader)
HashMap(java.util.HashMap)
Map(java.util.Map)
BidiMap(org.apache.commons.collections4.BidiMap)
DualHashBidiMap(org.apache.commons.collections4.bidimap.DualHashBidiMap)
Example 3 with Blast6Segment
use of cz1.ngs.model.Blast6Segment in project polyGembler by c-zhou.
the class Anchor0 method run.
@Override
public void run() {
// TODO Auto-generated method stub
final Map<String, Sequence> sub_seqs = Sequence.parseFastaFileAsMap(subject_file);
final Map<String, Sequence> qry_seqs = Sequence.parseFastaFileAsMap(query_file);
// find 'N/n's in subject/reference sequences
// which could have impact on parsing the blast records
final Map<String, TreeRangeSet<Integer>> sub_gaps = new HashMap<String, TreeRangeSet<Integer>>();
final Map<String, List<Blast6Segment>> anchored_records = new HashMap<String, List<Blast6Segment>>();
for (Map.Entry<String, Sequence> entry : sub_seqs.entrySet()) {
String seq_sn = entry.getKey();
String seq_str = entry.getValue().seq_str();
final TreeRangeSet<Integer> tmp_rangeSet = TreeRangeSet.create();
for (int j = 0; j < seq_str.length(); j++) {
if (seq_str.charAt(j) == 'N' || seq_str.charAt(j) == 'n')
// blast record is 1-based closed coordination
tmp_rangeSet.add(Range.closed(j + 1, j + 1).canonical(DiscreteDomain.integers()));
}
int seq_ln = seq_str.length();
final TreeRangeSet<Integer> range_set = TreeRangeSet.create();
for (Range<Integer> range : tmp_rangeSet.asRanges()) {
int lowerend = range.hasLowerBound() ? Math.max(0, range.lowerEndpoint() - gap_buff) : 0;
int upperend = range.hasUpperBound() ? Math.min(seq_ln, range.upperEndpoint() + gap_buff - 1) : seq_ln;
range_set.add(Range.closed(lowerend, upperend).canonical(DiscreteDomain.integers()));
}
sub_gaps.put(seq_sn, range_set);
// initialise an array list for each reference chromosome
anchored_records.put(seq_sn, new ArrayList<Blast6Segment>());
}
// parse blast records
// blast records buffer
final List<Blast6Segment> buff = new ArrayList<Blast6Segment>();
// selected blast records
final List<Blast6Segment> sel_recs = new ArrayList<Blast6Segment>();
// temp list
final List<Blast6Segment> tmp_records = new ArrayList<Blast6Segment>();
try {
BufferedReader br_blast = Utils.getBufferedReader(blast_out);
Blast6Segment tmp_record = Blast6Segment.blast6Segment(br_blast.readLine());
Blast6Segment primary_record, secondary_record;
String qry;
double qry_ln, aln_frac;
while (tmp_record != null) {
qry = tmp_record.qseqid();
qry_ln = qry_seqs.get(qry).seq_ln();
buff.clear();
buff.add(tmp_record);
while ((tmp_record = Blast6Segment.blast6Segment(br_blast.readLine())) != null && tmp_record.qseqid().equals(qry)) {
// filter by identity
if (tmp_record.pident() >= this.min_ident)
buff.add(tmp_record);
}
sel_recs.clear();
// merge collinear records
for (String sub : sub_seqs.keySet()) {
// get all records for subject/reference sequence sub_seq
tmp_records.clear();
for (Blast6Segment record : buff) if (record.sseqid().equals(sub))
tmp_records.add(record);
if (tmp_records.isEmpty())
continue;
// find alignment segments that can be deleted
// those that are subsets of larger alignment segments
// (Sstart, (sstart, send), Send) and (Qstart, (qstart, qend), Qend)
Collections.sort(tmp_records, new Blast6Segment.SegmentSizeComparator());
final Set<int[][]> ranges = new HashSet<int[][]>();
outerloop: for (int i = 0; i < tmp_records.size(); i++) {
primary_record = tmp_records.get(i);
int[][] range = new int[2][2];
if (primary_record.sstart() < primary_record.send()) {
range[0][0] = primary_record.sstart();
range[0][1] = primary_record.send();
} else {
range[0][0] = primary_record.send();
range[0][1] = primary_record.sstart();
}
if (primary_record.qstart() < primary_record.qend()) {
range[1][0] = primary_record.qstart();
range[1][1] = primary_record.qend();
} else {
range[1][0] = primary_record.qend();
range[1][1] = primary_record.qstart();
}
for (int[][] r : ranges) {
if (r[0][0] <= range[0][0] && r[0][1] >= range[0][1] && r[1][0] <= range[1][0] && r[1][1] >= range[1][1]) {
tmp_records.remove(i--);
continue outerloop;
}
}
ranges.add(range);
}
// TODO rewrite this part
/**
* // find collinear alignment segments that can be merged
* Collections.sort(tmp_records, new BlastRecord.SInterceptComparator());
*
* collinear_merged.clear();
* final List<Blast6Record> temp = new ArrayList<Blast6Record>();
* for(int i=0; i<tmp_records.size(); ) {
* Blast6Record record = tmp_records.get(i);
* double max_shift;
* temp.clear();
* temp.add(record);
*
* // find collinear alignment segments
* outerloop:
* while( (++i)<tmp_records.size() ) {
* record = tmp_records.get(i);
* // check if is collinear with other alignment segments
* for(Blast6Record r : temp) {
* max_shift = collinear_shift*
* Math.min(r.length(), record.length());
* if(BlastRecord.sdistance(r, record)<=max_shift &&
* BlastRecord.qdistance(r, record)<=max_shift &&
* BlastRecord.pdistance(r, record)<=max_shift) {
* temp.add(record);
* continue outerloop;
* }
* }
* break;
* }
*
* // merge collinear alignment segments
* int qstart = Integer.MAX_VALUE;
* int qend = Integer.MIN_VALUE;
* int sstart = Integer.MAX_VALUE;
* int send = Integer.MIN_VALUE;
* double pident = 0;
* int length = 0;
*
* // qstart is always smaller than qend
* for(int j=0; j<temp.size(); j++) {
* record = temp.get(j);
* if(record.qstart()<qstart) {
* qstart = record.qstart();
* sstart = record.sstart();
* }
* if(record.qend()>qend) {
* qend = record.qend();
* send = record.send();
* }
* if(record.pident()>pident)
* pident = record.pident();
* length += record.length();
* }
*
* collinear_merged.add(new Blast6Record(qry,sub,pident,length,-1,-1,qstart,qend,sstart,send,-1,-1));
* }
*/
// find collinear alignment segments that can be merged
// more accurate but slower
Collections.sort(tmp_records, new AlignmentSegment.SubjectCoordinationComparator());
Blast6Segment record;
for (int i = 0; i < tmp_records.size(); i++) {
primary_record = tmp_records.get(i);
for (int j = i + 1; j < tmp_records.size(); j++) {
secondary_record = tmp_records.get(j);
double max_shift = collinear_shift * Math.min(primary_record.length(), secondary_record.length());
if ((record = Blast6Segment.collinear(primary_record, secondary_record, max_shift)) != null) {
tmp_records.set(i, record);
tmp_records.remove(j);
--i;
break;
}
}
}
// process blast records that clipped by gaps
// (sstart, send)---(start2, send2)
// (sstart ... --- ... send2)
TreeRangeSet<Integer> sub_gap = sub_gaps.get(sub);
Collections.sort(tmp_records, new AlignmentSegment.SubjectCoordinationComparator());
for (int i = 0; i < tmp_records.size(); i++) {
primary_record = tmp_records.get(i);
if (sub_gap.contains(primary_record.true_send())) {
secondary_record = null;
int sec_j = -1;
for (int j = i + 1; j < tmp_records.size(); j++) {
if (tmp_records.get(j).true_sstart() >= primary_record.true_send()) {
secondary_record = tmp_records.get(j);
sec_j = j;
break;
}
}
if (secondary_record == null || AlignmentSegment.reverse(primary_record, secondary_record)) {
// reverse alignment segments
continue;
}
if (sub_gap.contains(secondary_record.true_sstart()) && sub_gap.rangeContaining(primary_record.true_send()).equals(sub_gap.rangeContaining(secondary_record.true_sstart()))) {
// clipping
// merge two alignment segments
double pident = Math.max(primary_record.pident(), secondary_record.pident());
int qstart = Math.min(primary_record.true_qstart(), secondary_record.true_qstart());
int qend = Math.max(primary_record.true_qend(), secondary_record.true_qend());
int sstart = Math.min(primary_record.true_sstart(), secondary_record.true_sstart());
int send = Math.max(primary_record.true_send(), secondary_record.true_send());
int length = qend - qstart + 1;
// replace primary record with merged record
// delete secondary record
Blast6Segment merged_record = primary_record.forward() ? new Blast6Segment(qry, sub, pident, length, -1, -1, qstart, qend, sstart, send, -1, -1) : new Blast6Segment(qry, sub, pident, length, -1, -1, qstart, qend, send, sstart, -1, -1);
tmp_records.set(i, merged_record);
tmp_records.remove(sec_j);
// the merged records need to be processed
--i;
}
}
}
// add to sel_recs
sel_recs.addAll(tmp_records);
}
// filter by alignment fraction
buff.clear();
buff.addAll(sel_recs);
sel_recs.clear();
for (Blast6Segment record : buff) {
if (record.length() / qry_ln >= this.min_frac)
sel_recs.add(record);
}
if (sel_recs.isEmpty()) {
// continue
continue;
}
// filter blast records
Collections.sort(sel_recs, new Blast6Segment.MatchIndentityComparator());
// process primary alignment
primary_record = sel_recs.get(0);
anchored_records.get(primary_record.sseqid()).add(primary_record);
aln_frac = primary_record.length() / qry_ln;
// and process
for (int i = 1; i < sel_recs.size(); i++) {
secondary_record = sel_recs.get(i);
if (secondary_record.pident() + this.diff_ident < primary_record.pident() || secondary_record.length() / qry_ln + this.diff_frac < aln_frac) {
break;
} else {
anchored_records.get(secondary_record.sseqid()).add(secondary_record);
}
}
}
br_blast.close();
for (Map.Entry<String, List<Blast6Segment>> entry : anchored_records.entrySet()) {
System.out.println(entry.getKey() + ": " + entry.getValue().size());
}
final BufferedWriter bw_map = Utils.getBufferedWriter(out_prefix + ".map");
final BufferedWriter bw_fa = Utils.getBufferedWriter(out_prefix + ".fa");
final Set<String> anchored_seqs = new HashSet<String>();
final List<String> sub_list = Sequence.parseSeqList(subject_file);
for (String sub_sn : sub_list) {
List<Blast6Segment> blast6_records = anchored_records.get(sub_sn);
int total = blast6_records.size(), count = 0;
// sort blast records
Collections.sort(blast6_records, new Blast6Segment.SubjectCoordinationComparator());
// consensus
int posUpto = 0, send_clip = 0;
int sstart, send, qstart, qend, qlen, tmp_int, qstart_clip, qend_clip, gap_size;
// distance to last 'N', start from next position to find longest common suffix-prefix
int prev_n = Integer.MAX_VALUE;
int mol_len = 0;
String qseq;
int nS, nQ;
// will form a pseudomolecule
StringBuilder seq_str = new StringBuilder();
for (Blast6Segment record : blast6_records) {
if (count++ % 10000 == 0)
myLogger.info(sub_sn + " " + count + "/" + total + " done.");
qlen = qry_seqs.get(record.qseqid()).seq_ln();
sstart = record.sstart();
send = record.send();
qstart = record.qstart();
qend = record.qend();
if (sstart > send) {
// make sure sstart<send
tmp_int = sstart;
sstart = send;
send = tmp_int;
tmp_int = qstart;
qstart = qend;
qend = tmp_int;
}
if (qstart > qend) {
qstart_clip = qlen - qstart;
qend_clip = qend - 1;
qseq = Sequence.revCompSeq(qry_seqs.get(record.qseqid()).seq_str());
} else {
qstart_clip = qstart - 1;
qend_clip = qlen - qend;
qseq = qry_seqs.get(record.qseqid()).seq_str();
}
if (send < posUpto || sstart < posUpto && qstart_clip > max_clip) {
// TODO process
continue;
}
// find longest suffix-prefix
nS = seq_str.length();
nQ = qseq.length();
int nO = Math.min(prev_n, Math.min(nS, nQ));
outerloop: for (; nO >= min_overlap; nO--) {
int nS_i = nS - nO;
for (int i = 0; i < nO; i++) {
if (seq_str.charAt(nS_i + i) != qseq.charAt(i))
continue outerloop;
}
break outerloop;
}
if (nO < min_overlap) {
// otherwise will insert a large GAP max(pseduo_distance, max_gap)
if (posUpto > 0) {
if (sstart <= posUpto) {
// if too much overlap, then treat it as a contradiction
if (posUpto - sstart > min_overlap) {
// discard
continue;
} else {
// insert a min_gap
gap_size = min_gap;
}
} else {
// estimate gap size
gap_size = (sstart - posUpto) - (send_clip + qstart_clip);
if (gap_size < max_gap)
gap_size = max_gap;
}
bw_map.write("GAP\t" + gap_size + "\t0\t" + gap_size + "\t+\t" + sub_sn + "\t" + mol_len + "\t" + (mol_len + gap_size) + "\n");
seq_str.append(Sequence.polyN(gap_size));
mol_len += gap_size;
}
bw_map.write(record.qseqid() + "\t" + qlen + "\t");
if (qstart > qend) {
// reverse
bw_map.write("0\t" + qlen + "\t-\t");
} else {
// forward
bw_map.write("0\t" + qlen + "\t+\t");
}
seq_str.append(qseq);
bw_map.write(sub_sn + "\t" + mol_len + "\t" + (mol_len + qlen) + "\n");
mol_len += qlen;
prev_n = qlen;
anchored_seqs.add(record.qseqid());
} else {
// overlap found
// will not insert gap
// ====================
// /-------\
// /----\
// calculate overlaps
// process overlap
qstart = nO;
if (qstart == qlen)
continue;
bw_map.write(record.qseqid() + "\t" + (qlen - qstart) + "\t");
if (qstart > qend) {
// reverse
bw_map.write(0 + "\t" + (qlen - qstart) + "\t-\t");
} else {
// forward
bw_map.write(qstart + "\t" + qlen + "\t+\t");
}
bw_map.write(sub_sn + "\t" + mol_len + "\t" + (mol_len + qlen - qstart) + "\n");
mol_len += qlen - qstart;
prev_n += qlen - qstart;
seq_str.append(qseq.substring(qstart));
anchored_seqs.add(record.qseqid());
}
posUpto = send;
send_clip = qend_clip;
}
if (seq_str.length() > 0)
bw_fa.write(Sequence.formatOutput(sub_sn, seq_str.toString()));
}
bw_fa.close();
bw_map.close();
final BufferedWriter bw_ufa = Utils.getBufferedWriter(out_prefix + "_unplaced.fa");
for (String seq : qry_seqs.keySet()) if (!anchored_seqs.contains(seq))
bw_ufa.write(qry_seqs.get(seq).formatOutput());
bw_ufa.close();
} catch (IOException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
Also used :
TreeRangeSet(com.google.common.collect.TreeRangeSet)
HashMap(java.util.HashMap)
Blast6Segment(cz1.ngs.model.Blast6Segment)
ArrayList(java.util.ArrayList)
BufferedWriter(java.io.BufferedWriter)
ArrayList(java.util.ArrayList)
List(java.util.List)
HashSet(java.util.HashSet)
Sequence(cz1.ngs.model.Sequence)
IOException(java.io.IOException)
AlignmentSegment(cz1.ngs.model.AlignmentSegment)
BufferedReader(java.io.BufferedReader)
HashMap(java.util.HashMap)
Map(java.util.Map)
Example 4 with Blast6Segment
use of cz1.ngs.model.Blast6Segment in project polyGembler by c-zhou.
the class Anchor1 method run.
// private static DijkstraShortestPath<Integer, DefaultWeightedEdge> dijkstra_paths;
@Override
public void run() {
// TODO Auto-generated method stub
long tic = System.nanoTime();
sub_seqs = Sequence.parseFastaFileAsMap(subject_file);
qry_seqs = Sequence.parseFastaFileWithRevCmpAsMap(query_file);
// this.makeAssemblyGraph(query_file);
this.makeAssemblyGraph(query_file);
bfs = new BFSShortestPath<Integer, DefaultWeightedEdge>(assembly_graph);
// dijkstra_paths = new DijkstraShortestPath<Integer, DefaultWeightedEdge>(assembly_graph);
// find 'N/n's in subject/reference sequences
// which could have impact on parsing the blast records
final Map<String, TreeRangeSet<Integer>> sub_gaps = new HashMap<String, TreeRangeSet<Integer>>();
final Map<String, List<Blast6Segment>> anchored_records = new HashMap<String, List<Blast6Segment>>();
for (Map.Entry<String, Sequence> entry : sub_seqs.entrySet()) {
String seq_sn = entry.getKey();
String seq_str = entry.getValue().seq_str();
final TreeRangeSet<Integer> tmp_rangeSet = TreeRangeSet.create();
for (int j = 0; j < seq_str.length(); j++) {
if (seq_str.charAt(j) == 'N' || seq_str.charAt(j) == 'n')
// blast record is 1-based closed coordination
tmp_rangeSet.add(Range.closed(j + 1, j + 1).canonical(DiscreteDomain.integers()));
}
int seq_ln = seq_str.length();
final TreeRangeSet<Integer> range_set = TreeRangeSet.create();
for (Range<Integer> range : tmp_rangeSet.asRanges()) {
int lowerend = range.hasLowerBound() ? Math.max(0, range.lowerEndpoint() - gap_buff) : 0;
int upperend = range.hasUpperBound() ? Math.min(seq_ln, range.upperEndpoint() + gap_buff - 1) : seq_ln;
range_set.add(Range.closed(lowerend, upperend).canonical(DiscreteDomain.integers()));
}
sub_gaps.put(seq_sn, range_set);
// initialise an array list for each reference chromosome
anchored_records.put(seq_sn, new ArrayList<Blast6Segment>());
}
// parse blast records
// blast records buffer
final List<Blast6Segment> buff = new ArrayList<Blast6Segment>();
// selected blast records
final List<Blast6Segment> sel_recs = new ArrayList<Blast6Segment>();
// temp list
final List<Blast6Segment> tmp_records = new ArrayList<Blast6Segment>();
try {
BufferedReader br_blast = Utils.getBufferedReader(blast_out);
Blast6Segment tmp_record = Blast6Segment.blast6Segment(br_blast.readLine());
Blast6Segment primary_record, secondary_record;
String qry;
double qry_ln, aln_frac;
while (tmp_record != null) {
qry = tmp_record.qseqid();
qry_ln = qry_seqs.get(qry).seq_ln();
buff.clear();
buff.add(tmp_record);
while ((tmp_record = Blast6Segment.blast6Segment(br_blast.readLine())) != null && tmp_record.qseqid().equals(qry)) {
// filter by identity
if (tmp_record.pident() >= this.min_ident)
buff.add(tmp_record);
}
sel_recs.clear();
// merge collinear records
for (String sub : sub_seqs.keySet()) {
// get all records for subject/reference sequence sub_seq
tmp_records.clear();
for (Blast6Segment record : buff) if (record.sseqid().equals(sub))
tmp_records.add(record);
if (tmp_records.isEmpty())
continue;
// find alignment segments that can be deleted
// those that are subsets of larger alignment segments
// (Sstart, (sstart, send), Send) and (Qstart, (qstart, qend), Qend)
Collections.sort(tmp_records, new Blast6Segment.SegmentSizeComparator());
final Set<int[][]> ranges = new HashSet<int[][]>();
outerloop: for (int i = 0; i < tmp_records.size(); i++) {
primary_record = tmp_records.get(i);
int[][] range = new int[2][2];
if (primary_record.sstart() < primary_record.send()) {
range[0][0] = primary_record.sstart();
range[0][1] = primary_record.send();
} else {
range[0][0] = primary_record.send();
range[0][1] = primary_record.sstart();
}
if (primary_record.qstart() < primary_record.qend()) {
range[1][0] = primary_record.qstart();
range[1][1] = primary_record.qend();
} else {
range[1][0] = primary_record.qend();
range[1][1] = primary_record.qstart();
}
for (int[][] r : ranges) {
if (r[0][0] <= range[0][0] && r[0][1] >= range[0][1] && r[1][0] <= range[1][0] && r[1][1] >= range[1][1]) {
tmp_records.remove(i--);
continue outerloop;
}
}
ranges.add(range);
}
// TODO rewrite this part
/**
* // find collinear alignment segments that can be merged
* Collections.sort(tmp_records, new BlastRecord.SInterceptComparator());
*
* collinear_merged.clear();
* final List<Blast6Record> temp = new ArrayList<Blast6Record>();
* for(int i=0; i<tmp_records.size(); ) {
* Blast6Record record = tmp_records.get(i);
* double max_shift;
* temp.clear();
* temp.add(record);
*
* // find collinear alignment segments
* outerloop:
* while( (++i)<tmp_records.size() ) {
* record = tmp_records.get(i);
* // check if is collinear with other alignment segments
* for(Blast6Record r : temp) {
* max_shift = collinear_shift*
* Math.min(r.length(), record.length());
* if(BlastRecord.sdistance(r, record)<=max_shift &&
* BlastRecord.qdistance(r, record)<=max_shift &&
* BlastRecord.pdistance(r, record)<=max_shift) {
* temp.add(record);
* continue outerloop;
* }
* }
* break;
* }
*
* // merge collinear alignment segments
* int qstart = Integer.MAX_VALUE;
* int qend = Integer.MIN_VALUE;
* int sstart = Integer.MAX_VALUE;
* int send = Integer.MIN_VALUE;
* double pident = 0;
* int length = 0;
*
* // qstart is always smaller than qend
* for(int j=0; j<temp.size(); j++) {
* record = temp.get(j);
* if(record.qstart()<qstart) {
* qstart = record.qstart();
* sstart = record.sstart();
* }
* if(record.qend()>qend) {
* qend = record.qend();
* send = record.send();
* }
* if(record.pident()>pident)
* pident = record.pident();
* length += record.length();
* }
*
* collinear_merged.add(new Blast6Record(qry,sub,pident,length,-1,-1,qstart,qend,sstart,send,-1,-1));
* }
*/
// find collinear alignment segments that can be merged
// more accurate but slower
Collections.sort(tmp_records, new AlignmentSegment.SubjectCoordinationComparator());
Blast6Segment record;
for (int i = 0; i < tmp_records.size(); i++) {
primary_record = tmp_records.get(i);
for (int j = i + 1; j < tmp_records.size(); j++) {
secondary_record = tmp_records.get(j);
double max_shift = collinear_shift * Math.min(primary_record.length(), secondary_record.length());
if ((record = Blast6Segment.collinear(primary_record, secondary_record, max_shift)) != null) {
tmp_records.set(i, record);
tmp_records.remove(j);
--i;
break;
}
}
}
// process blast records that clipped by gaps
// (sstart, send)---(start2, send2)
// (sstart ... --- ... send2)
TreeRangeSet<Integer> sub_gap = sub_gaps.get(sub);
Collections.sort(tmp_records, new AlignmentSegment.SubjectCoordinationComparator());
for (int i = 0; i < tmp_records.size(); i++) {
primary_record = tmp_records.get(i);
if (sub_gap.contains(primary_record.true_send())) {
secondary_record = null;
int sec_j = -1;
for (int j = i + 1; j < tmp_records.size(); j++) {
if (tmp_records.get(j).true_sstart() >= primary_record.true_send()) {
secondary_record = tmp_records.get(j);
sec_j = j;
break;
}
}
if (secondary_record == null || AlignmentSegment.reverse(primary_record, secondary_record)) {
// reverse alignment segments
continue;
}
if (sub_gap.contains(secondary_record.true_sstart()) && sub_gap.rangeContaining(primary_record.true_send()).equals(sub_gap.rangeContaining(secondary_record.true_sstart()))) {
// clipping
// merge two alignment segments
double pident = Math.max(primary_record.pident(), secondary_record.pident());
int qstart = Math.min(primary_record.true_qstart(), secondary_record.true_qstart());
int qend = Math.max(primary_record.true_qend(), secondary_record.true_qend());
int sstart = Math.min(primary_record.true_sstart(), secondary_record.true_sstart());
int send = Math.max(primary_record.true_send(), secondary_record.true_send());
int length = qend - qstart + 1;
// replace primary record with merged record
// delete secondary record
Blast6Segment merged_record = primary_record.forward() ? new Blast6Segment(qry, sub, pident, length, -1, -1, qstart, qend, sstart, send, -1, -1) : new Blast6Segment(qry, sub, pident, length, -1, -1, qstart, qend, send, sstart, -1, -1);
tmp_records.set(i, merged_record);
tmp_records.remove(sec_j);
// the merged records need to be processed
--i;
}
}
}
// add to sel_recs
sel_recs.addAll(tmp_records);
}
// filter by alignment fraction
buff.clear();
buff.addAll(sel_recs);
sel_recs.clear();
for (Blast6Segment record : buff) {
if (record.length() / qry_ln >= this.min_frac)
sel_recs.add(record);
}
if (sel_recs.isEmpty()) {
// continue
continue;
}
// filter blast records
Collections.sort(sel_recs, new Blast6Segment.MatchIndentityComparator());
// process primary alignment
primary_record = sel_recs.get(0);
anchored_records.get(primary_record.sseqid()).add(primary_record);
aln_frac = primary_record.length() / qry_ln;
// and process
for (int i = 1; i < sel_recs.size(); i++) {
secondary_record = sel_recs.get(i);
if (secondary_record.pident() + this.diff_ident < primary_record.pident() || secondary_record.length() / qry_ln + this.diff_frac < aln_frac) {
break;
} else {
anchored_records.get(secondary_record.sseqid()).add(secondary_record);
}
}
}
br_blast.close();
for (Map.Entry<String, List<Blast6Segment>> entry : anchored_records.entrySet()) {
System.out.println(entry.getKey() + ": " + entry.getValue().size());
}
final BufferedWriter bw_map = Utils.getBufferedWriter(out_prefix + ".map");
final BufferedWriter bw_fa = Utils.getBufferedWriter(out_prefix + ".fa");
final Set<String> anchored_seqs = new HashSet<String>();
final List<String> sub_list = Sequence.parseSeqList(subject_file);
final List<GraphPath<Integer, DefaultWeightedEdge>> paths = new ArrayList<GraphPath<Integer, DefaultWeightedEdge>>();
for (String sub_sn : sub_list) {
blast6_records = anchored_records.get(sub_sn);
int nV = blast6_records.size(), count = 0;
// sort blast records
Collections.sort(blast6_records, new Blast6Segment.SubjectCoordinationComparator());
// consensus
int posUpto = 0, send_clip = 0;
int sstart, send, qstart, qend, qlen, tmp_int, qstart_clip, qend_clip, gap_size;
// distance to last 'N', start from next position to find longest common suffix-prefix
int prev_n = Integer.MAX_VALUE;
int mol_len = 0;
int bfs_dist;
String qseq;
int nS, nQ;
// first step: construct super scaffold
// will form a tree graph indicating the path through the contigs
// convert contig names to integer indices
// one contig could end up with multiple indices due to repeats
Map<Integer, String> ss_coordinate = new HashMap<Integer, String>();
int index = 0;
for (Blast6Segment record : blast6_records) ss_coordinate.put(index++, record.qseqid() + (record.forward() ? "" : "'"));
int qseqid1, qseqid2;
String os;
List<Double> dist_stats = new ArrayList<Double>();
for (int i = 0; i != nV; i++) {
qseqid1 = contig_coordinate.getKey(ss_coordinate.get(i));
os = blast6_records.get(i).qseqid() + (blast6_records.get(i).forward() ? "" : "'");
System.out.print(Utils.fixedLengthPaddingString(os, 40) + "\t");
double distance = Double.MAX_VALUE;
for (int j = 1; j <= window_size; j++) {
if (i + j >= nV) {
System.out.print(Utils.fixedLengthPaddingString("", 10) + ",\t");
continue;
}
qseqid2 = contig_coordinate.getKey(ss_coordinate.get(i + j));
GraphPath<Integer, DefaultWeightedEdge> e = bfs.getPath(qseqid1, qseqid2);
// GraphPath<Integer, DefaultWeightedEdge> e = dijkstra_paths.getPath( qseqid1, qseqid2 );
if (e != null) {
double d = e.getLength();
if (distance > d)
distance = d;
}
int l = 0;
if (e != null) {
List<Integer> vertices = e.getVertexList();
for (int k = 1; k < vertices.size() - 1; k++) l += qry_seqs.get(contig_coordinate.get(vertices.get(k))).seq_ln();
l -= (vertices.size() - 2) * this.kmer_size;
}
System.out.print((e == null ? Utils.fixedLengthPaddingString("Inf " + l, 10) + ",\t" : Utils.fixedLengthPaddingString(e.getLength() + " " + l, 10) + ",\t"));
}
System.out.println();
dist_stats.add(distance);
}
double[] dist_arr = ArrayUtils.toPrimitive(dist_stats.toArray(new Double[dist_stats.size()]));
Arrays.sort(dist_arr);
Percentile percentile = new Percentile();
double q1 = percentile.evaluate(dist_arr, 25);
double q3 = percentile.evaluate(dist_arr, 75);
// upper bound for outliers
// q3+1.5*IQR
double outlier = q3 + 1.5 * (q3 - q1);
System.out.println("Outlier upper bound: " + outlier);
// construct a tree graph
pseudo_tree = new DirectedWeightedPseudograph<Integer, DefaultWeightedEdge>(DefaultWeightedEdge.class);
for (int i : ss_coordinate.keySet()) pseudo_tree.addVertex(i);
Blast6Segment record1, record2;
// select the nearest neighbour(s)
for (int i = 0; i != nV; i++) {
System.out.println(i);
record1 = blast6_records.get(i);
send = record1.true_send();
qseqid1 = contig_coordinate.getKey(ss_coordinate.get(i));
paths.clear();
bfs_dist = Integer.MAX_VALUE;
for (int j = i + 1; j < nV; j++) {
record2 = blast6_records.get(j);
sstart = record2.true_sstart();
if (send < sstart)
break;
qseqid2 = contig_coordinate.getKey(ss_coordinate.get(j));
GraphPath<Integer, DefaultWeightedEdge> e = bfs.getPath(qseqid1, qseqid2);
// GraphPath<Integer, DefaultWeightedEdge> e = dijkstra_paths.getPath( qseqid1, qseqid2 );
if (e != null) {
tmp_int = e.getLength();
if (tmp_int == 0)
continue;
if (tmp_int < bfs_dist)
paths.clear();
paths.add(e);
bfs_dist = tmp_int;
}
}
if (paths.isEmpty())
continue;
for (GraphPath<Integer, DefaultWeightedEdge> path : paths) pseudo_tree.setEdgeWeight(pseudo_tree.addEdge(path.getStartVertex(), path.getEndVertex()), 1d);
}
// secondly fill gaps
long toc = System.nanoTime();
System.out.println(toc - tic + " nano secs.");
if (true)
throw new RuntimeException("!!!");
// second step: construct pseudo chromosomes
// 1. choose the first contig
int firstv = 0;
while (true) {
// int v2 = next(firstv+1);
if (true)
break;
}
// 2. extend pseudo chromosomes
StringBuilder seq_str = new StringBuilder();
for (int v = firstv; v < nV - 1; v++) {
if (++count % 10000 == 0)
myLogger.info(sub_sn + " " + count + "/" + nV + " done.");
Blast6Segment record = blast6_records.get(v);
qlen = qry_seqs.get(record.qseqid()).seq_ln();
sstart = record.sstart();
send = record.send();
qstart = record.qstart();
qend = record.qend();
if (sstart > send) {
// make sure sstart<send
tmp_int = sstart;
sstart = send;
send = tmp_int;
tmp_int = qstart;
qstart = qend;
qend = tmp_int;
}
if (qstart > qend) {
qstart_clip = qlen - qstart;
qend_clip = qend - 1;
qseq = Sequence.revCompSeq(qry_seqs.get(record.qseqid()).seq_str());
} else {
qstart_clip = qstart - 1;
qend_clip = qlen - qend;
qseq = qry_seqs.get(record.qseqid()).seq_str();
}
if (send < posUpto || sstart < posUpto && qstart_clip > max_clip) {
// TODO process
continue;
}
// find longest suffix-prefix
nS = seq_str.length();
nQ = qseq.length();
int nO = Math.min(prev_n, Math.min(nS, nQ));
outerloop: for (; nO >= min_overlap; nO--) {
int nS_i = nS - nO;
for (int i = 0; i < nO; i++) {
if (seq_str.charAt(nS_i + i) != qseq.charAt(i))
continue outerloop;
}
break outerloop;
}
if (nO < min_overlap) {
// otherwise will insert a large GAP max(pseduo_distance, max_gap)
if (posUpto > 0) {
if (sstart <= posUpto) {
// if too much overlap, then treat it as a contradiction
if (posUpto - sstart > min_overlap) {
// discard
continue;
} else {
// insert a min_gap
gap_size = min_gap;
}
} else {
// estimate gap size
gap_size = (sstart - posUpto) - (send_clip + qstart_clip);
if (gap_size < max_gap)
gap_size = max_gap;
}
bw_map.write("GAP\t" + gap_size + "\t0\t" + gap_size + "\t+\t" + sub_sn + "\t" + mol_len + "\t" + (mol_len + gap_size) + "\n");
seq_str.append(Sequence.polyN(gap_size));
mol_len += gap_size;
}
bw_map.write(record.qseqid() + "\t" + qlen + "\t");
if (qstart > qend) {
// reverse
bw_map.write("0\t" + qlen + "\t-\t");
} else {
// forward
bw_map.write("0\t" + qlen + "\t+\t");
}
seq_str.append(qseq);
bw_map.write(sub_sn + "\t" + mol_len + "\t" + (mol_len + qlen) + "\n");
mol_len += qlen;
prev_n = qlen;
anchored_seqs.add(record.qseqid());
} else {
// overlap found
// will not insert gap
// ====================
// /-------\
// /----\
// calculate overlaps
// process overlap
qstart = nO;
if (qstart == qlen)
continue;
bw_map.write(record.qseqid() + "\t" + (qlen - qstart) + "\t");
if (qstart > qend) {
// reverse
bw_map.write(0 + "\t" + (qlen - qstart) + "\t-\t");
} else {
// forward
bw_map.write(qstart + "\t" + qlen + "\t+\t");
}
bw_map.write(sub_sn + "\t" + mol_len + "\t" + (mol_len + qlen - qstart) + "\n");
mol_len += qlen - qstart;
prev_n += qlen - qstart;
seq_str.append(qseq.substring(qstart));
anchored_seqs.add(record.qseqid());
}
posUpto = send;
send_clip = qend_clip;
}
if (seq_str.length() > 0)
bw_fa.write(Sequence.formatOutput(sub_sn, seq_str.toString()));
}
bw_fa.close();
bw_map.close();
final BufferedWriter bw_ufa = Utils.getBufferedWriter(out_prefix + "_unplaced.fa");
for (String seq : qry_seqs.keySet()) if (!anchored_seqs.contains(seq))
bw_ufa.write(qry_seqs.get(seq).formatOutput());
bw_ufa.close();
} catch (IOException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
Also used :
Percentile(org.apache.commons.math3.stat.descriptive.rank.Percentile)
TreeRangeSet(com.google.common.collect.TreeRangeSet)
HashMap(java.util.HashMap)
Blast6Segment(cz1.ngs.model.Blast6Segment)
ArrayList(java.util.ArrayList)
GraphPath(org.jgrapht.GraphPath)
BufferedWriter(java.io.BufferedWriter)
ArrayList(java.util.ArrayList)
List(java.util.List)
HashSet(java.util.HashSet)
Sequence(cz1.ngs.model.Sequence)
IOException(java.io.IOException)
DefaultWeightedEdge(org.jgrapht.graph.DefaultWeightedEdge)
AlignmentSegment(cz1.ngs.model.AlignmentSegment)
BufferedReader(java.io.BufferedReader)
HashMap(java.util.HashMap)
Map(java.util.Map)
BidiMap(org.apache.commons.collections4.BidiMap)
DualHashBidiMap(org.apache.commons.collections4.bidimap.DualHashBidiMap)
Aggregations
Blast6Segment (cz1.ngs.model.Blast6Segment)4
Sequence (cz1.ngs.model.Sequence)4
BufferedReader (java.io.BufferedReader)4
BufferedWriter (java.io.BufferedWriter)4
IOException (java.io.IOException)4
HashMap (java.util.HashMap)4
HashSet (java.util.HashSet)4
Map (java.util.Map)4
TreeRangeSet (com.google.common.collect.TreeRangeSet)3
AlignmentSegment (cz1.ngs.model.AlignmentSegment)3
ArrayList (java.util.ArrayList)3
List (java.util.List)3
BidiMap (org.apache.commons.collections4.BidiMap)2
DualHashBidiMap (org.apache.commons.collections4.bidimap.DualHashBidiMap)2
DefaultWeightedEdge (org.jgrapht.graph.DefaultWeightedEdge)2
FileReader (java.io.FileReader)1
Percentile (org.apache.commons.math3.stat.descriptive.rank.Percentile)1
GraphPath (org.jgrapht.GraphPath)1
