Examples with MapqMetrics gridss.analysis.MapqMetrics used on opensource projects

Example 1 with MapqMetrics

use of gridss.analysis.MapqMetrics in project gridss by PapenfussLab.

the class IdsvSamFileMetricsCollectorTest method should_calc_max_fragment_size.

@Test
public void should_calc_max_fragment_size() {
    IdsvSamFileMetricsCollector c = new IdsvSamFileMetricsCollector(null);
    c.acceptRecord(Read(0, 1, "100M"), null);
    c.acceptRecord(RP(0, 1, 2, 1)[0], null);
    c.acceptRecord(RP(0, 1, 7, 5)[0], null);
    // should ignore this as it's not a proper pair
    SAMRecord r = RP(0, 1, 100, 5)[0];
    r.setProperPairFlag(false);
    c.acceptRecord(r, null);
    // 12345678901234567890
    // ----> <----
    c.acceptRecord(Read(0, 1, "100M"), null);
    MetricsFile<IdsvMetrics, Integer> idsv = new MetricsFile<IdsvMetrics, Integer>();
    MetricsFile<InsertSizeMetrics, Integer> is = new MetricsFile<InsertSizeMetrics, Integer>();
    MetricsFile<CigarDetailMetrics, Integer> sc = new MetricsFile<CigarDetailMetrics, Integer>();
    MetricsFile<MapqMetrics, Integer> mq = new MetricsFile<MapqMetrics, Integer>();
    c.finish(is, idsv, mq, sc);
    assertEquals(11, (int) ((IdsvMetrics) idsv.getMetrics().get(0)).MAX_PROPER_PAIR_FRAGMENT_LENGTH);
    c = new IdsvSamFileMetricsCollector(null);
    // mate before
    r = RP(0, 1, 100, 5)[1];
    c.acceptRecord(r, null);
    c.finish(is, idsv, mq, sc);
    assertEquals(11, (int) ((IdsvMetrics) idsv.getMetrics().get(0)).MAX_PROPER_PAIR_FRAGMENT_LENGTH);
}

Example 2 with MapqMetrics

use of gridss.analysis.MapqMetrics in project gridss by PapenfussLab.

the class EmpiricalReferenceLikelihoodModelTest method should_score_read_pairs_correctly.

@Test
public void should_score_read_pairs_correctly() {
    IdsvSamFileMetrics metrics = new IdsvSamFileMetrics(new InsertSizeMetrics() {

        {
            MEAN_INSERT_SIZE = 2;
            MEDIAN_INSERT_SIZE = 2;
            MIN_INSERT_SIZE = 1;
            MAX_INSERT_SIZE = 3;
            MEDIAN_ABSOLUTE_DEVIATION = 1;
        }
    }, new IdsvMetrics() {

        {
            MAX_READ_LENGTH = 100;
            MAX_PROPER_PAIR_FRAGMENT_LENGTH = 2;
            MIN_PROPER_PAIR_FRAGMENT_LENGTH = 2;
            READ_PAIRS = 13;
            READ_PAIRS_ONE_MAPPED = 2;
            READ_PAIRS_ZERO_MAPPED = 1;
            READ_PAIRS_BOTH_MAPPED = READ_PAIRS - READ_PAIRS_ONE_MAPPED - READ_PAIRS_ZERO_MAPPED;
            READS = 2 * READ_PAIRS;
            MAPPED_READS = READS - READ_PAIRS_ONE_MAPPED - 2 * READ_PAIRS_ZERO_MAPPED;
        }
    }, new MapqMetrics() {

        {
        }
    }, new InsertSizeDistribution(new int[] { 1, 2, 3 }, new double[] { 1, 8, 1 }), new ArrayList<CigarDetailMetrics>());
    assertEquals(10, model.scoreReadPair(metrics, 3, 1000, 1000), 0.001);
}

Example 3 with MapqMetrics

use of gridss.analysis.MapqMetrics in project gridss by PapenfussLab.

the class TestHelper method IDSV.

public IdsvMetrics IDSV(Collection<SAMRecord> input) {
    IdsvSamFileMetricsCollector c = new IdsvSamFileMetricsCollector(null);
    for (SAMRecord r : input) {
        c.acceptRecord(r, null);
    }
    MetricsFile<IdsvMetrics, Integer> idsv = new MetricsFile<IdsvMetrics, Integer>();
    MetricsFile<InsertSizeMetrics, Integer> is = new MetricsFile<InsertSizeMetrics, Integer>();
    MetricsFile<CigarDetailMetrics, Integer> sc = new MetricsFile<CigarDetailMetrics, Integer>();
    MetricsFile<MapqMetrics, Integer> mqm = new MetricsFile<MapqMetrics, Integer>();
    c.finish(is, idsv, mqm, sc);
    IdsvMetrics metrics = idsv.getMetrics().get(0);
    return metrics;
}

Example 4 with MapqMetrics

use of gridss.analysis.MapqMetrics in project gridss by PapenfussLab.

the class IdsvSamFileMetrics method getMapqMetrics.

public static MapqMetrics getMapqMetrics(File mapqMetricsFile) {
    if (mapqMetricsFile == null)
        return null;
    MapqMetrics bestMetrics = null;
    for (MapqMetrics metric : Iterables.filter(MetricsFile.readBeans(mapqMetricsFile), MapqMetrics.class)) {
        if (metric.SAMPLE == null && metric.LIBRARY == null && metric.READ_GROUP == null) {
            if (bestMetrics == null || bestMetrics.MAPPED_READS < metric.MAPPED_READS) {
                bestMetrics = metric;
            }
        }
    }
    if (bestMetrics == null) {
        bestMetrics = new MapqMetrics();
        log.error(String.format("No mapq metrics found in %s.", mapqMetricsFile));
    }
    return bestMetrics;
}

Example 5 with MapqMetrics

use of gridss.analysis.MapqMetrics in project gridss by PapenfussLab.

the class IdsvSamFileMetricsTest method wrapper_inner_metrics.

@Test
public void wrapper_inner_metrics() {
    IdsvMetrics im = new IdsvMetrics();
    InsertSizeMetrics ism = new InsertSizeMetrics();
    MapqMetrics mqm = new MapqMetrics();
    List<CigarDetailMetrics> sc = new ArrayList<CigarDetailMetrics>();
    InsertSizeDistribution isd = new InsertSizeDistribution(new int[] { 1 }, new double[] { 1 });
    IdsvSamFileMetrics metrics = new IdsvSamFileMetrics(ism, im, mqm, isd, sc);
    assertEquals(im, metrics.getIdsvMetrics());
    assertEquals(isd, metrics.getInsertSizeDistribution());
    assertEquals(ism, metrics.getInsertSizeMetrics());
    assertEquals(sc, metrics.getCigarDetailMetrics());
    assertEquals(mqm, metrics.getMapqMetrics());
}