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Example 6 with CompositeImage

use of ij.CompositeImage in project GDSC-SMLM by aherbert.

the class PulseActivationAnalysis method displayComposite.

private void displayComposite(ImageProcessor[] images, String name) {
    // We do not yet know the size
    ImageStack stack = null;
    for (int i = 0; i < images.length; i++) {
        ImageProcessor ip = images[i];
        if (stack == null)
            stack = new ImageStack(ip.getWidth(), ip.getHeight());
        ip.setColorModel(null);
        stack.addSlice("C" + (i + 1), ip);
    }
    // Create a composite
    ImagePlus imp = new ImagePlus(name, stack);
    imp.setDimensions(images.length, 1, 1);
    CompositeImage ci = new CompositeImage(imp, IJ.COMPOSITE);
    // Make it easier to see
    //ij.plugin.ContrastEnhancerce = new ij.plugin.ContrastEnhancer();
    //double saturated = 0.35;
    //ce.stretchHistogram(ci, saturated);
    autoAdjust(ci, ci.getProcessor());
    imp = WindowManager.getImage(name);
    if (imp != null && imp.isComposite()) {
        ci.setMode(imp.getCompositeMode());
        imp.setImage(ci);
        imp.getWindow().toFront();
    } else {
        ci.show();
        imp = ci;
    }
    if (WindowManager.getWindow("Channels") == null) {
        IJ.run("Channels Tool...");
        Window w = WindowManager.getWindow("Channels");
        if (w == null)
            return;
        Window w2 = imp.getWindow();
        if (w2 == null)
            return;
        java.awt.Point p = w2.getLocation();
        p.x += w2.getWidth();
        w.setLocation(p);
    }
}
Also used : Window(java.awt.Window) ImageProcessor(ij.process.ImageProcessor) ImageStack(ij.ImageStack) CompositeImage(ij.CompositeImage) ImagePlus(ij.ImagePlus)

Example 7 with CompositeImage

use of ij.CompositeImage in project bioformats by openmicroscopy.

the class Exporter method run.

// -- Exporter API methods --
/**
 * Executes the plugin.
 */
public void run() {
    String outfile = null;
    Boolean splitZ = null;
    Boolean splitC = null;
    Boolean splitT = null;
    Boolean padded = null;
    Boolean saveRoi = null;
    String compression = null;
    Boolean windowless = Boolean.FALSE;
    if (plugin.arg != null) {
        outfile = Macro.getValue(plugin.arg, "outfile", null);
        String z = Macro.getValue(plugin.arg, "splitZ", null);
        String c = Macro.getValue(plugin.arg, "splitC", null);
        String t = Macro.getValue(plugin.arg, "splitT", null);
        String zeroPad = Macro.getValue(plugin.arg, "padded", null);
        String sr = Macro.getValue(plugin.arg, "saveRoi", null);
        compression = Macro.getValue(plugin.arg, "compression", null);
        String id = Macro.getValue(plugin.arg, "imageid", null);
        splitZ = z == null ? null : Boolean.valueOf(z);
        splitC = c == null ? null : Boolean.valueOf(c);
        splitT = t == null ? null : Boolean.valueOf(t);
        padded = zeroPad == null ? null : Boolean.valueOf(zeroPad);
        saveRoi = sr == null ? null : Boolean.valueOf(sr);
        if (id != null) {
            try {
                int imageID = Integer.parseInt(id);
                ImagePlus plus = WindowManager.getImage(imageID);
                if (plus != null)
                    imp = plus;
            } catch (Exception e) {
            // nothing to do, we use the current imagePlus
            }
        }
        String w = Macro.getValue(plugin.arg, "windowless", null);
        if (w != null) {
            windowless = Boolean.valueOf(w);
        }
        plugin.arg = null;
    }
    if (outfile == null) {
        String options = Macro.getOptions();
        if (options != null) {
            String save = Macro.getValue(options, "save", null);
            if (save != null)
                outfile = save;
        }
    }
    // create a temporary file if window less
    if (windowless && (outfile == null || outfile.length() == 0)) {
        File tmp = null;
        try {
            String name = removeExtension(imp.getTitle());
            String n = name + ".ome.tif";
            tmp = File.createTempFile(name, ".ome.tif");
            File p = tmp.getParentFile();
            File[] list = p.listFiles();
            // make sure we delete a previous tmp file with same name if any
            if (list != null) {
                File toDelete = null;
                for (int i = 0; i < list.length; i++) {
                    if (list[i].getName().equals(n)) {
                        toDelete = list[i];
                        break;
                    }
                }
                if (toDelete != null) {
                    toDelete.delete();
                }
            }
            outfile = new File(p, n).getAbsolutePath();
            if (Recorder.record)
                Recorder.recordPath("outputfile", outfile);
            IJ.log("exporter outputfile " + outfile);
        } catch (Exception e) {
        // fall back to window mode.
        } finally {
            if (tmp != null)
                tmp.delete();
        }
    }
    File f = null;
    if (outfile == null || outfile.length() == 0) {
        // open a dialog prompting for the filename to save
        // NB: Copied and adapted from ij.io.SaveDIalog.jSaveDispatchThread,
        // so that the save dialog has a file filter for choosing output format.
        String dir = null, name = null;
        JFileChooser fc = GUITools.buildFileChooser(new ImageWriter(), false);
        fc.setDialogTitle("Bio-Formats Exporter");
        String defaultDir = OpenDialog.getDefaultDirectory();
        if (defaultDir != null)
            fc.setCurrentDirectory(new File(defaultDir));
        // set OME-TIFF as the default output format
        FileFilter[] ff = fc.getChoosableFileFilters();
        FileFilter defaultFilter = null;
        for (int i = 0; i < ff.length; i++) {
            if (ff[i] instanceof ExtensionFileFilter) {
                ExtensionFileFilter eff = (ExtensionFileFilter) ff[i];
                if (i == 0 || eff.getExtension().equals("ome.tif")) {
                    defaultFilter = eff;
                    break;
                }
            }
        }
        if (defaultFilter != null)
            fc.setFileFilter(defaultFilter);
        int returnVal = fc.showSaveDialog(IJ.getInstance());
        if (returnVal != JFileChooser.APPROVE_OPTION) {
            Macro.abort();
            return;
        }
        f = fc.getSelectedFile();
        dir = fc.getCurrentDirectory().getPath() + File.separator;
        name = fc.getName(f);
        if (f.exists()) {
            int ret = JOptionPane.showConfirmDialog(fc, "The file " + f.getName() + " already exists. \n" + "Would you like to replace it?", "Replace?", JOptionPane.YES_NO_OPTION, JOptionPane.WARNING_MESSAGE);
            if (ret != JOptionPane.OK_OPTION)
                f = null;
        } else {
            // ensure filename matches selected filter
            FileFilter filter = fc.getFileFilter();
            if (filter instanceof ExtensionFileFilter) {
                ExtensionFileFilter eff = (ExtensionFileFilter) filter;
                String[] ext = eff.getExtensions();
                String lName = name.toLowerCase();
                boolean hasExtension = false;
                for (int i = 0; i < ext.length; i++) {
                    if (lName.endsWith("." + ext[i])) {
                        hasExtension = true;
                        break;
                    }
                }
                if (!hasExtension && ext.length > 0) {
                    // append chosen extension
                    name = name + "." + ext[0];
                }
                f = fc.getSelectedFile();
                String filePath = f.getAbsolutePath();
                if (!filePath.endsWith("." + ext[0])) {
                    f = new File(filePath + '.' + ext[0]);
                }
                if (f.exists()) {
                    int ret1 = JOptionPane.showConfirmDialog(fc, "The file " + f.getName() + " already exists. \n" + "Would you like to replace it?", "Replace?", JOptionPane.YES_NO_OPTION, JOptionPane.WARNING_MESSAGE);
                    if (ret1 != JOptionPane.OK_OPTION)
                        f = null;
                }
            }
        }
        if (f == null)
            Macro.abort();
        else {
            // do some ImageJ bookkeeping
            OpenDialog.setDefaultDirectory(dir);
            if (Recorder.record)
                Recorder.recordPath("save", dir + name);
        }
        if (dir == null || name == null)
            return;
        outfile = new File(dir, name).getAbsolutePath();
        if (outfile == null)
            return;
    }
    if (windowless) {
        if (splitZ == null)
            splitZ = Boolean.FALSE;
        if (splitC == null)
            splitC = Boolean.FALSE;
        if (splitT == null)
            splitT = Boolean.FALSE;
        if (padded == null)
            padded = Boolean.FALSE;
    }
    if (splitZ == null || splitC == null || splitT == null) {
        // ask if we want to export multiple files
        GenericDialog multiFile = new GenericDialog("Bio-Formats Exporter - Multiple Files");
        multiFile.addCheckbox("Write_each_Z_section to a separate file", false);
        multiFile.addCheckbox("Write_each_timepoint to a separate file", false);
        multiFile.addCheckbox("Write_each_channel to a separate file", false);
        multiFile.addCheckbox("Use zero padding for filename indexes", false);
        multiFile.showDialog();
        splitZ = multiFile.getNextBoolean();
        splitT = multiFile.getNextBoolean();
        splitC = multiFile.getNextBoolean();
        padded = multiFile.getNextBoolean();
        if (multiFile.wasCanceled())
            return;
    }
    try (IFormatWriter w = new ImageWriter().getWriter(outfile)) {
        int ptype = 0;
        int channels = 1;
        switch(imp.getType()) {
            case ImagePlus.GRAY8:
            case ImagePlus.COLOR_256:
                ptype = FormatTools.UINT8;
                break;
            case ImagePlus.COLOR_RGB:
                channels = 3;
                ptype = FormatTools.UINT8;
                break;
            case ImagePlus.GRAY16:
                ptype = FormatTools.UINT16;
                break;
            case ImagePlus.GRAY32:
                ptype = FormatTools.FLOAT;
                break;
        }
        String title = imp.getTitle();
        w.setWriteSequentially(true);
        FileInfo fi = imp.getOriginalFileInfo();
        String xml = fi == null ? null : fi.description == null ? null : fi.description.indexOf("xml") == -1 ? null : fi.description;
        OMEXMLService service = null;
        IMetadata store = null;
        try {
            ServiceFactory factory = new ServiceFactory();
            service = factory.getInstance(OMEXMLService.class);
            store = service.createOMEXMLMetadata(xml);
        } catch (DependencyException de) {
        } catch (ServiceException se) {
        }
        if (store == null)
            IJ.error("OME-XML Java library not found.");
        OMEXMLMetadataRoot root = (OMEXMLMetadataRoot) store.getRoot();
        if (root.sizeOfROIList() > 0) {
            while (root.sizeOfROIList() > 0) {
                ROI roi = root.getROI(0);
                root.removeROI(roi);
            }
            store.setRoot(root);
        }
        if (xml == null) {
            store.createRoot();
        } else if (store.getImageCount() > 1) {
            // the original dataset had multiple series
            // we need to modify the IMetadata to represent the correct series
            ArrayList<Integer> matchingSeries = new ArrayList<Integer>();
            for (int series = 0; series < store.getImageCount(); series++) {
                String type = store.getPixelsType(series).toString();
                int pixelType = FormatTools.pixelTypeFromString(type);
                if (pixelType == ptype) {
                    String imageName = store.getImageName(series);
                    if (title.indexOf(imageName) >= 0) {
                        matchingSeries.add(series);
                    }
                }
            }
            int series = 0;
            if (matchingSeries.size() > 1) {
                for (int i = 0; i < matchingSeries.size(); i++) {
                    int index = matchingSeries.get(i);
                    String name = store.getImageName(index);
                    boolean valid = true;
                    for (int j = 0; j < matchingSeries.size(); j++) {
                        if (i != j) {
                            String compName = store.getImageName(matchingSeries.get(j));
                            if (compName.indexOf(name) >= 0) {
                                valid = false;
                                break;
                            }
                        }
                    }
                    if (valid) {
                        series = index;
                        break;
                    }
                }
            } else if (matchingSeries.size() == 1)
                series = matchingSeries.get(0);
            ome.xml.model.Image exportImage = root.getImage(series);
            List<ome.xml.model.Image> allImages = root.copyImageList();
            for (ome.xml.model.Image img : allImages) {
                if (!img.equals(exportImage)) {
                    root.removeImage(img);
                }
            }
            store.setRoot(root);
        }
        store.setPixelsSizeX(new PositiveInteger(imp.getWidth()), 0);
        store.setPixelsSizeY(new PositiveInteger(imp.getHeight()), 0);
        store.setPixelsSizeZ(new PositiveInteger(imp.getNSlices()), 0);
        store.setPixelsSizeC(new PositiveInteger(channels * imp.getNChannels()), 0);
        store.setPixelsSizeT(new PositiveInteger(imp.getNFrames()), 0);
        if (store.getImageID(0) == null) {
            store.setImageID(MetadataTools.createLSID("Image", 0), 0);
        }
        if (store.getPixelsID(0) == null) {
            store.setPixelsID(MetadataTools.createLSID("Pixels", 0), 0);
        }
        // reset the pixel type, unless the only change is signedness
        // this prevents problems if the user changed the bit depth of the image
        boolean applyCalibrationFunction = false;
        try {
            int originalType = -1;
            if (store.getPixelsType(0) != null) {
                originalType = FormatTools.pixelTypeFromString(store.getPixelsType(0).toString());
            }
            if (ptype != originalType && (store.getPixelsType(0) == null || !FormatTools.isSigned(originalType) || FormatTools.getBytesPerPixel(originalType) != FormatTools.getBytesPerPixel(ptype))) {
                store.setPixelsType(PixelType.fromString(FormatTools.getPixelTypeString(ptype)), 0);
            } else if (FormatTools.isSigned(originalType)) {
                applyCalibrationFunction = true;
            }
        } catch (EnumerationException e) {
        }
        if (store.getPixelsBinDataCount(0) == 0 || store.getPixelsBinDataBigEndian(0, 0) == null) {
            store.setPixelsBinDataBigEndian(Boolean.FALSE, 0, 0);
        }
        if (store.getPixelsDimensionOrder(0) == null) {
            try {
                store.setPixelsDimensionOrder(DimensionOrder.fromString(ORDER), 0);
            } catch (EnumerationException e) {
            }
        }
        LUT[] luts = new LUT[imp.getNChannels()];
        for (int c = 0; c < imp.getNChannels(); c++) {
            if (c >= store.getChannelCount(0) || store.getChannelID(0, c) == null) {
                String lsid = MetadataTools.createLSID("Channel", 0, c);
                store.setChannelID(lsid, 0, c);
            }
            store.setChannelSamplesPerPixel(new PositiveInteger(channels), 0, 0);
            if (imp instanceof CompositeImage) {
                luts[c] = ((CompositeImage) imp).getChannelLut(c + 1);
            }
        }
        Calibration cal = imp.getCalibration();
        store.setPixelsPhysicalSizeX(FormatTools.getPhysicalSizeX(cal.pixelWidth), 0);
        store.setPixelsPhysicalSizeY(FormatTools.getPhysicalSizeY(cal.pixelHeight), 0);
        store.setPixelsPhysicalSizeZ(FormatTools.getPhysicalSizeZ(cal.pixelDepth), 0);
        store.setPixelsTimeIncrement(new Time(new Double(cal.frameInterval), UNITS.SECOND), 0);
        if (imp.getImageStackSize() != imp.getNChannels() * imp.getNSlices() * imp.getNFrames()) {
            if (!windowless) {
                IJ.showMessageWithCancel("Bio-Formats Exporter Warning", "The number of planes in the stack (" + imp.getImageStackSize() + ") does not match the number of expected planes (" + (imp.getNChannels() * imp.getNSlices() * imp.getNFrames()) + ")." + "\nIf you select 'OK', only " + imp.getImageStackSize() + " planes will be exported. If you wish to export all of the " + "planes,\nselect 'Cancel' and convert the Image5D window " + "to a stack.");
            }
            store.setPixelsSizeZ(new PositiveInteger(imp.getImageStackSize()), 0);
            store.setPixelsSizeC(new PositiveInteger(1), 0);
            store.setPixelsSizeT(new PositiveInteger(1), 0);
        }
        Object info = imp.getProperty("Info");
        if (info != null) {
            String imageInfo = info.toString();
            if (imageInfo != null) {
                String[] lines = imageInfo.split("\n");
                for (String line : lines) {
                    int eq = line.lastIndexOf("=");
                    if (eq > 0) {
                        String key = line.substring(0, eq).trim();
                        String value = line.substring(eq + 1).trim();
                        if (key.endsWith("BitsPerPixel")) {
                            w.setValidBitsPerPixel(Integer.parseInt(value));
                            break;
                        }
                    }
                }
            }
        }
        // NB: Animation rate code copied from ij.plugin.Animator#doOptions().
        final int rate;
        if (cal.fps != 0.0) {
            rate = (int) cal.fps;
        } else if (cal.frameInterval != 0.0 && cal.getTimeUnit().equals("sec")) {
            rate = (int) (1.0 / cal.frameInterval);
        } else {
            // NB: Code from ij.plugin.Animator#animationRate initializer.
            // The value is 7 by default in ImageJ, so must be 7 here as well.
            rate = (int) Prefs.getDouble(Prefs.FPS, 7.0);
        }
        if (rate > 0)
            w.setFramesPerSecond(rate);
        String[] outputFiles = new String[] { outfile };
        int sizeZ = store.getPixelsSizeZ(0).getValue();
        int sizeC = store.getPixelsSizeC(0).getValue();
        int sizeT = store.getPixelsSizeT(0).getValue();
        if (splitZ || splitC || splitT) {
            int nFiles = 1;
            if (splitZ) {
                nFiles *= sizeZ;
            }
            if (splitC) {
                nFiles *= sizeC;
            }
            if (splitT) {
                nFiles *= sizeT;
            }
            outputFiles = new String[nFiles];
            int dot = outfile.indexOf(".", outfile.lastIndexOf(File.separator));
            String base = outfile.substring(0, dot);
            String ext = outfile.substring(dot);
            int nextFile = 0;
            for (int z = 0; z < (splitZ ? sizeZ : 1); z++) {
                for (int c = 0; c < (splitC ? sizeC : 1); c++) {
                    for (int t = 0; t < (splitT ? sizeT : 1); t++) {
                        int index = FormatTools.getIndex(ORDER, sizeZ, sizeC, sizeT, sizeZ * sizeC * sizeT, z, c, t);
                        String pattern = base + (splitZ ? "_Z%z" : "") + (splitC ? "_C%c" : "") + (splitT ? "_T%t" : "") + ext;
                        outputFiles[nextFile++] = FormatTools.getFilename(0, index, store, pattern, padded);
                    }
                }
            }
        }
        if (!w.getFormat().startsWith("OME")) {
            if (splitZ) {
                store.setPixelsSizeZ(new PositiveInteger(1), 0);
            }
            if (splitC) {
                store.setPixelsSizeC(new PositiveInteger(1), 0);
            }
            if (splitT) {
                store.setPixelsSizeT(new PositiveInteger(1), 0);
            }
        }
        // prompt for options
        String[] codecs = w.getCompressionTypes();
        ImageProcessor proc = imp.getImageStack().getProcessor(1);
        Image firstImage = proc.createImage();
        firstImage = AWTImageTools.makeBuffered(firstImage, proc.getColorModel());
        int thisType = AWTImageTools.getPixelType((BufferedImage) firstImage);
        if (proc instanceof ColorProcessor) {
            thisType = FormatTools.UINT8;
        } else if (proc instanceof ShortProcessor) {
            thisType = FormatTools.UINT16;
        }
        boolean notSupportedType = !w.isSupportedType(thisType);
        if (notSupportedType) {
            IJ.error("Pixel type (" + FormatTools.getPixelTypeString(thisType) + ") not supported by this format.");
            return;
        }
        if (codecs != null && codecs.length > 1) {
            boolean selected = false;
            if (compression != null) {
                for (int i = 0; i < codecs.length; i++) {
                    if (codecs[i].equals(compression)) {
                        selected = true;
                        break;
                    }
                }
            }
            if (!selected && !windowless) {
                GenericDialog gd = new GenericDialog("Bio-Formats Exporter Options");
                gd.addChoice("Compression type: ", codecs, codecs[0]);
                if (saveRoi != null) {
                    gd.addCheckbox("Export ROIs", saveRoi.booleanValue());
                } else {
                    gd.addCheckbox("Export ROIs", true);
                }
                gd.showDialog();
                saveRoi = gd.getNextBoolean();
                if (gd.wasCanceled())
                    return;
                compression = gd.getNextChoice();
            }
        }
        boolean in = false;
        if (outputFiles.length > 1) {
            for (int i = 0; i < outputFiles.length; i++) {
                if (new File(outputFiles[i]).exists()) {
                    in = true;
                    break;
                }
            }
        }
        if (in && !windowless) {
            int ret1 = JOptionPane.showConfirmDialog(null, "Some files already exist. \n" + "Would you like to replace them?", "Replace?", JOptionPane.YES_NO_OPTION, JOptionPane.WARNING_MESSAGE);
            if (ret1 != JOptionPane.OK_OPTION) {
                return;
            }
            // Delete the files overwrite does not correctly work
            for (int i = 0; i < outputFiles.length; i++) {
                new File(outputFiles[i]).delete();
            }
        }
        // delete the file.
        if (f != null)
            f.delete();
        if (compression != null) {
            w.setCompression(compression);
        }
        // Save ROI's
        if (saveRoi != null && saveRoi.booleanValue()) {
            ROIHandler.saveROIs(store);
        }
        w.setMetadataRetrieve(store);
        // convert and save slices
        int size = imp.getImageStackSize();
        ImageStack is = imp.getImageStack();
        boolean doStack = w.canDoStacks() && size > 1;
        int start = doStack ? 0 : imp.getCurrentSlice() - 1;
        int end = doStack ? size : start + 1;
        boolean littleEndian = false;
        if (w.getMetadataRetrieve().getPixelsBigEndian(0) != null) {
            littleEndian = !w.getMetadataRetrieve().getPixelsBigEndian(0).booleanValue();
        } else if (w.getMetadataRetrieve().getPixelsBinDataCount(0) == 0) {
            littleEndian = !w.getMetadataRetrieve().getPixelsBinDataBigEndian(0, 0).booleanValue();
        }
        byte[] plane = null;
        w.setInterleaved(false);
        int[] no = new int[outputFiles.length];
        for (int i = start; i < end; i++) {
            if (doStack) {
                BF.status(false, "Saving plane " + (i + 1) + "/" + size);
                BF.progress(false, i, size);
            } else
                BF.status(false, "Saving image");
            proc = is.getProcessor(i + 1);
            if (proc instanceof RecordedImageProcessor) {
                proc = ((RecordedImageProcessor) proc).getChild();
            }
            int x = proc.getWidth();
            int y = proc.getHeight();
            if (proc instanceof ByteProcessor) {
                if (applyCalibrationFunction) {
                    // don't alter 'pixels' directly as that will
                    // affect the open ImagePlus
                    byte[] pixels = (byte[]) proc.getPixels();
                    plane = new byte[pixels.length];
                    float[] calibration = proc.getCalibrationTable();
                    for (int pixel = 0; pixel < pixels.length; pixel++) {
                        plane[pixel] = (byte) calibration[pixels[pixel] & 0xff];
                    }
                } else {
                    plane = (byte[]) proc.getPixels();
                }
            } else if (proc instanceof ShortProcessor) {
                short[] pixels = (short[]) proc.getPixels();
                if (applyCalibrationFunction) {
                    // don't alter 'pixels' directly as that will
                    // affect the open ImagePlus
                    plane = new byte[pixels.length * 2];
                    float[] calibration = proc.getCalibrationTable();
                    for (int pixel = 0; pixel < pixels.length; pixel++) {
                        short v = (short) calibration[pixels[pixel] & 0xffff];
                        DataTools.unpackBytes(v, plane, pixel * 2, 2, littleEndian);
                    }
                } else {
                    plane = DataTools.shortsToBytes(pixels, littleEndian);
                }
            } else if (proc instanceof FloatProcessor) {
                plane = DataTools.floatsToBytes((float[]) proc.getPixels(), littleEndian);
            } else if (proc instanceof ColorProcessor) {
                byte[][] pix = new byte[3][x * y];
                ((ColorProcessor) proc).getRGB(pix[0], pix[1], pix[2]);
                plane = new byte[3 * x * y];
                System.arraycopy(pix[0], 0, plane, 0, x * y);
                System.arraycopy(pix[1], 0, plane, x * y, x * y);
                System.arraycopy(pix[2], 0, plane, 2 * x * y, x * y);
                if (i == start) {
                    sizeC /= 3;
                }
            }
            int fileIndex = 0;
            if (doStack) {
                int[] coords = FormatTools.getZCTCoords(ORDER, sizeZ, sizeC, sizeT, size, i);
                int realZ = sizeZ;
                int realC = sizeC;
                int realT = sizeT;
                if (!splitZ) {
                    coords[0] = 0;
                    realZ = 1;
                }
                if (!splitC) {
                    coords[1] = 0;
                    realC = 1;
                }
                if (!splitT) {
                    coords[2] = 0;
                    realT = 1;
                }
                fileIndex = FormatTools.getIndex(ORDER, realZ, realC, realT, realZ * realC * realT, coords[0], coords[1], coords[2]);
            }
            if (notSupportedType) {
                IJ.error("Pixel type not supported by this format.");
            } else {
                w.changeOutputFile(outputFiles[fileIndex]);
                int currentChannel = FormatTools.getZCTCoords(ORDER, sizeZ, sizeC, sizeT, imp.getStackSize(), i)[1];
                if (luts[currentChannel] != null) {
                    // expand to 16-bit LUT if necessary
                    int bpp = FormatTools.getBytesPerPixel(thisType);
                    if (bpp == 1) {
                        w.setColorModel(luts[currentChannel]);
                    } else if (bpp == 2) {
                        int lutSize = luts[currentChannel].getMapSize();
                        byte[][] lut = new byte[3][lutSize];
                        luts[currentChannel].getReds(lut[0]);
                        luts[currentChannel].getGreens(lut[1]);
                        luts[currentChannel].getBlues(lut[2]);
                        short[][] newLut = new short[3][65536];
                        int bins = newLut[0].length / lut[0].length;
                        for (int c = 0; c < newLut.length; c++) {
                            for (int q = 0; q < newLut[c].length; q++) {
                                int index = q / bins;
                                newLut[c][q] = (short) ((lut[c][index] * lut[0].length) + (q % bins));
                            }
                        }
                        w.setColorModel(new Index16ColorModel(16, newLut[0].length, newLut, littleEndian));
                    }
                } else if (!proc.isDefaultLut()) {
                    w.setColorModel(proc.getColorModel());
                }
                w.saveBytes(no[fileIndex]++, plane);
            }
        }
        w.close();
    } catch (FormatException e) {
        WindowTools.reportException(e);
    } catch (IOException e) {
        WindowTools.reportException(e);
    }
}
Also used : ServiceFactory(loci.common.services.ServiceFactory) ArrayList(java.util.ArrayList) Image(java.awt.Image) BufferedImage(java.awt.image.BufferedImage) CompositeImage(ij.CompositeImage) OMEXMLService(loci.formats.services.OMEXMLService) Index16ColorModel(loci.formats.gui.Index16ColorModel) ImageProcessor(ij.process.ImageProcessor) RecordedImageProcessor(loci.plugins.util.RecordedImageProcessor) IMetadata(loci.formats.meta.IMetadata) FileInfo(ij.io.FileInfo) List(java.util.List) ArrayList(java.util.ArrayList) ImageStack(ij.ImageStack) FloatProcessor(ij.process.FloatProcessor) DependencyException(loci.common.services.DependencyException) ROI(ome.xml.model.ROI) FormatException(loci.formats.FormatException) IFormatWriter(loci.formats.IFormatWriter) JFileChooser(javax.swing.JFileChooser) ServiceException(loci.common.services.ServiceException) OMEXMLMetadataRoot(ome.xml.meta.OMEXMLMetadataRoot) File(java.io.File) ExtensionFileFilter(loci.formats.gui.ExtensionFileFilter) EnumerationException(ome.xml.model.enums.EnumerationException) ByteProcessor(ij.process.ByteProcessor) ImageWriter(loci.formats.ImageWriter) Time(ome.units.quantity.Time) ColorProcessor(ij.process.ColorProcessor) CompositeImage(ij.CompositeImage) GenericDialog(ij.gui.GenericDialog) RecordedImageProcessor(loci.plugins.util.RecordedImageProcessor) ExtensionFileFilter(loci.formats.gui.ExtensionFileFilter) FileFilter(javax.swing.filechooser.FileFilter) PositiveInteger(ome.xml.model.primitives.PositiveInteger) LUT(ij.process.LUT) Calibration(ij.measure.Calibration) IOException(java.io.IOException) ImagePlus(ij.ImagePlus) ServiceException(loci.common.services.ServiceException) DependencyException(loci.common.services.DependencyException) EnumerationException(ome.xml.model.enums.EnumerationException) FormatException(loci.formats.FormatException) IOException(java.io.IOException) ShortProcessor(ij.process.ShortProcessor) PositiveInteger(ome.xml.model.primitives.PositiveInteger)

Example 8 with CompositeImage

use of ij.CompositeImage in project bioformats by openmicroscopy.

the class Read_Image method applyLookupTables.

private ImagePlus applyLookupTables(IFormatReader r, ImagePlus imp, byte[][][] lookupTable) {
    // this requires ImageJ v1.39 or higher
    if (r.isIndexed()) {
        CompositeImage composite = new CompositeImage(imp, CompositeImage.COLOR);
        for (int c = 0; c < r.getSizeC(); c++) {
            composite.setPosition(c + 1, 1, 1);
            LUT lut = new LUT(lookupTable[c][0], lookupTable[c][1], lookupTable[c][2]);
            composite.setChannelLut(lut);
        }
        composite.setPosition(1, 1, 1);
        return composite;
    }
    return imp;
}
Also used : CompositeImage(ij.CompositeImage) LUT(ij.process.LUT)

Example 9 with CompositeImage

use of ij.CompositeImage in project bioformats by openmicroscopy.

the class AutoscaleTest method test.

/**
 * Tester for composite (multi-channel) images.
 */
private void test(int pixelType, boolean autoscale, boolean crop, double calib, double min1, double max1, double min2, double max2, double min3, double max3) {
    final ImagePlus imp = setup(pixelType, autoscale, crop, calib, 3);
    final CompositeImage ci = (CompositeImage) imp;
    final LUT lut1 = ci.getChannelLut(1);
    final LUT lut2 = ci.getChannelLut(2);
    final LUT lut3 = ci.getChannelLut(3);
    assertEquals(min1 - calib, lut1.min, 0.0);
    assertEquals(max1 - calib, lut1.max, 0.0);
    assertEquals(min2 - calib, lut2.min, 0.0);
    assertEquals(max2 - calib, lut2.max, 0.0);
    assertEquals(min3 - calib, lut3.min, 0.0);
    assertEquals(max3 - calib, lut3.max, 0.0);
}
Also used : CompositeImage(ij.CompositeImage) LUT(ij.process.LUT) ImagePlus(ij.ImagePlus)

Example 10 with CompositeImage

use of ij.CompositeImage in project bioformats by openmicroscopy.

the class AutoscaleTest method dump.

private void dump(String pix, int sizeC, boolean autoscale, boolean crop, ImagePlus imp) {
    if (!LOGGER.isDebugEnabled()) {
        return;
    }
    StringBuilder sb = new StringBuilder();
    sb.append("pixelType=");
    // fixed width
    for (int s = pix.length(); s < 6; s++) sb.append(" ");
    sb.append(pix);
    sb.append(", sizeC=");
    sb.append(sizeC);
    sb.append(", autoscale=");
    sb.append(autoscale ? " true" : "false");
    sb.append(", crop=");
    sb.append(crop ? " true" : "false");
    sb.append(": image=");
    if (imp instanceof CompositeImage) {
        final CompositeImage ci = (CompositeImage) imp;
        sb.append("composite");
        for (int c = 0; c < sizeC; c++) {
            final LUT lut = ci.getChannelLut(c + 1);
            sb.append("; min");
            sb.append(c);
            sb.append("=");
            sb.append(lut.min);
            sb.append("; max");
            sb.append(c);
            sb.append("=");
            sb.append(lut.max);
        }
    } else {
        sb.append("  vanilla");
        final double min = imp.getDisplayRangeMin();
        final double max = imp.getDisplayRangeMax();
        sb.append("; min=");
        sb.append(min);
        sb.append("; max=");
        sb.append(max);
    }
    final Calibration cal = imp.getCalibration();
    if (cal.getFunction() == Calibration.STRAIGHT_LINE) {
        sb.append("; calibration=");
        double[] coeffs = cal.getCoefficients();
        sb.append(coeffs[0]);
    }
    LOGGER.debug(sb.toString());
}
Also used : CompositeImage(ij.CompositeImage) LUT(ij.process.LUT) Calibration(ij.measure.Calibration)

Aggregations

CompositeImage (ij.CompositeImage)20 ImagePlus (ij.ImagePlus)15 IOException (java.io.IOException)10 FormatException (loci.formats.FormatException)10 LUT (ij.process.LUT)9 ImageStack (ij.ImageStack)5 Calibration (ij.measure.Calibration)4 ImageProcessor (ij.process.ImageProcessor)4 VirtualImagePlus (loci.plugins.util.VirtualImagePlus)3 GenericDialog (ij.gui.GenericDialog)2 ColorProcessor (ij.process.ColorProcessor)2 Window (java.awt.Window)2 IndexColorModel (java.awt.image.IndexColorModel)2 ImageProcessorReader (loci.plugins.util.ImageProcessorReader)2 FileInfo (ij.io.FileInfo)1 ByteProcessor (ij.process.ByteProcessor)1 FloatProcessor (ij.process.FloatProcessor)1 ShortProcessor (ij.process.ShortProcessor)1 Color (java.awt.Color)1 Image (java.awt.Image)1