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Example 11 with ImageReader

use of loci.formats.ImageReader in project bioformats by openmicroscopy.

the class ImageReaderTest method testOptionsImplicit.

@Test(dataProvider = "levels")
public void testOptionsImplicit(MetadataLevel level) throws Exception {
    ImageReader reader = new ImageReader();
    reader.getMetadataOptions().setMetadataLevel(level);
    reader.setId("test.fake");
    MetadataLevel rLevel = reader.getReader().getMetadataOptions().getMetadataLevel();
    assertEquals(rLevel, level);
    reader.close();
}
Also used : ImageReader(loci.formats.ImageReader) MetadataLevel(loci.formats.in.MetadataLevel) Test(org.testng.annotations.Test)

Example 12 with ImageReader

use of loci.formats.ImageReader in project bioformats by openmicroscopy.

the class SPWModelReaderTest method testSetId.

@Test
public void testSetId() throws Exception {
    reader = new MinMaxCalculator(new ChannelSeparator(new ChannelFiller(new ImageReader())));
    metadata = new OMEXMLMetadataImpl();
    reader.setMetadataStore(metadata);
    reader.setId(temporaryFile.getAbsolutePath());
}
Also used : MinMaxCalculator(loci.formats.MinMaxCalculator) ChannelFiller(loci.formats.ChannelFiller) ImageReader(loci.formats.ImageReader) ChannelSeparator(loci.formats.ChannelSeparator) OMEXMLMetadataImpl(loci.formats.ome.OMEXMLMetadataImpl) Test(org.testng.annotations.Test)

Example 13 with ImageReader

use of loci.formats.ImageReader in project bioformats by openmicroscopy.

the class SPWModelReaderTest method testSetIdWithNoLightSources.

@Test
public void testSetIdWithNoLightSources() throws Exception {
    readerWithNoLightSources = new MinMaxCalculator(new ChannelSeparator(new ChannelFiller(new ImageReader())));
    metadataWithNoLightSources = new OMEXMLMetadataImpl();
    readerWithNoLightSources.setMetadataStore(metadataWithNoLightSources);
    readerWithNoLightSources.setId(temporaryFileWithNoLightSources.getAbsolutePath());
}
Also used : MinMaxCalculator(loci.formats.MinMaxCalculator) ChannelFiller(loci.formats.ChannelFiller) ImageReader(loci.formats.ImageReader) ChannelSeparator(loci.formats.ChannelSeparator) OMEXMLMetadataImpl(loci.formats.ome.OMEXMLMetadataImpl) Test(org.testng.annotations.Test)

Example 14 with ImageReader

use of loci.formats.ImageReader in project bioformats by openmicroscopy.

the class Mass_Importer method run.

public void run(String arg) {
    // prompt user for directory to process
    DirectoryChooser dc = new DirectoryChooser("Bio-Formats Mass Importer");
    String dirPath = dc.getDirectory();
    // create a list of files we have already processed
    HashSet<String> done = new HashSet<String>();
    // list of files to actually open with Bio-Formats Importer
    ArrayList<String> filesToOpen = new ArrayList<String>();
    // process all files in the chosen directory
    File dir = new File(dirPath);
    File[] files = dir.listFiles();
    IJ.showStatus("Scanning directory");
    // image reader object, for testing whether a file is in a supported format
    try (ImageReader tester = new ImageReader()) {
        for (int i = 0; i < files.length; i++) {
            String id = files[i].getAbsolutePath();
            IJ.showProgress((double) i / files.length);
            // skip files that have already been processed
            if (done.contains(id))
                continue;
            // skip unsupported files
            if (!tester.isThisType(id, false))
                continue;
            // use FilePattern to group files with similar names
            String name = files[i].getName();
            FilePattern fp = new FilePattern(name, dirPath);
            // get a list of all files part of this group, and mark them as done
            String[] used = fp.getFiles();
            for (int j = 0; j < used.length; j++) done.add(used[j]);
            filesToOpen.add(id);
        }
    } catch (IOException e) {
        IJ.error("Sorry, an error while closing ImageReader: " + e.getMessage());
    }
    IJ.showProgress(1.0);
    IJ.showStatus("");
    // confirm that user wants to proceed in opening the file groups
    int numToOpen = filesToOpen.size();
    if (numToOpen == 0) {
        IJ.showMessage("No file groups found.");
        return;
    }
    String groups = numToOpen == 1 ? "1 file group" : (numToOpen + " file groups");
    YesNoCancelDialog confirm = new YesNoCancelDialog(IJ.getInstance(), "Bio-Formats Mass Importer", "Found " + groups + " in directory '" + dirPath + "'; proceed?");
    if (!confirm.yesPressed())
        return;
    // launch the Bio-Formats Importer plugin to open each group of files
    for (int i = 0; i < numToOpen; i++) {
        String id = (String) filesToOpen.get(i);
        String params = "location=[Local machine] " + "windowless=true " + "groupFiles=true " + "id=[" + id + "] ";
        new LociImporter().run(params);
    }
    IJ.showStatus("");
}
Also used : ArrayList(java.util.ArrayList) IOException(java.io.IOException) LociImporter(loci.plugins.LociImporter) YesNoCancelDialog(ij.gui.YesNoCancelDialog) FilePattern(loci.formats.FilePattern) ImageReader(loci.formats.ImageReader) File(java.io.File) DirectoryChooser(ij.io.DirectoryChooser) HashSet(java.util.HashSet)

Example 15 with ImageReader

use of loci.formats.ImageReader in project bioformats by openmicroscopy.

the class ImageConverter method testConvert.

// -- Utility methods --
/**
 * A utility method for converting a file from the command line.
 */
public boolean testConvert(IFormatWriter writer, String[] args) throws FormatException, IOException {
    nextOutputIndex.clear();
    options.setValidate(validate);
    writer.setMetadataOptions(options);
    firstTile = true;
    boolean success = parseArgs(args);
    if (!success) {
        return false;
    }
    if (printVersion) {
        CommandLineTools.printVersion();
        return true;
    }
    CommandLineTools.runUpgradeCheck(args);
    if (in == null || out == null) {
        printUsage();
        return false;
    }
    if (new Location(out).exists()) {
        if (overwrite == null) {
            LOGGER.warn("Output file {} exists.", out);
            LOGGER.warn("Do you want to overwrite it? ([y]/n)");
            BufferedReader r = new BufferedReader(new InputStreamReader(System.in, Constants.ENCODING));
            String choice = r.readLine().trim().toLowerCase();
            overwrite = !choice.startsWith("n");
        }
        if (!overwrite) {
            LOGGER.warn("Exiting; next time, please specify an output file that " + "does not exist.");
            return false;
        } else {
            new Location(out).delete();
        }
    }
    if (map != null)
        Location.mapId(in, map);
    long start = System.currentTimeMillis();
    LOGGER.info(in);
    reader = new ImageReader();
    if (stitch) {
        reader = new FileStitcher(reader);
        Location f = new Location(in);
        String pat = null;
        if (!f.exists()) {
            pat = in;
        } else {
            pat = FilePattern.findPattern(f);
        }
        if (pat != null)
            in = pat;
    }
    if (separate)
        reader = new ChannelSeparator(reader);
    if (merge)
        reader = new ChannelMerger(reader);
    if (fill)
        reader = new ChannelFiller(reader);
    minMax = null;
    if (autoscale) {
        reader = new MinMaxCalculator(reader);
        minMax = (MinMaxCalculator) reader;
    }
    reader.setMetadataOptions(options);
    reader.setGroupFiles(group);
    reader.setMetadataFiltered(true);
    reader.setOriginalMetadataPopulated(true);
    OMEXMLService service = null;
    try {
        ServiceFactory factory = new ServiceFactory();
        service = factory.getInstance(OMEXMLService.class);
        reader.setMetadataStore(service.createOMEXMLMetadata());
    } catch (DependencyException de) {
        throw new MissingLibraryException(OMEXMLServiceImpl.NO_OME_XML_MSG, de);
    } catch (ServiceException se) {
        throw new FormatException(se);
    }
    reader.setId(in);
    MetadataStore store = reader.getMetadataStore();
    MetadataTools.populatePixels(store, reader, false, false);
    boolean dimensionsSet = true;
    if (width == 0 || height == 0) {
        // otherwise default to series 0
        if (series >= 0) {
            reader.setSeries(series);
        }
        width = reader.getSizeX();
        height = reader.getSizeY();
        dimensionsSet = false;
    }
    if (channel >= reader.getEffectiveSizeC()) {
        throw new FormatException("Invalid channel '" + channel + "' (" + reader.getEffectiveSizeC() + " channels in source file)");
    }
    if (timepoint >= reader.getSizeT()) {
        throw new FormatException("Invalid timepoint '" + timepoint + "' (" + reader.getSizeT() + " timepoints in source file)");
    }
    if (zSection >= reader.getSizeZ()) {
        throw new FormatException("Invalid Z section '" + zSection + "' (" + reader.getSizeZ() + " Z sections in source file)");
    }
    if (store instanceof MetadataRetrieve) {
        try {
            String xml = service.getOMEXML(service.asRetrieve(store));
            OMEXMLMetadataRoot root = (OMEXMLMetadataRoot) store.getRoot();
            IMetadata meta = service.createOMEXMLMetadata(xml);
            if (series >= 0) {
                Image exportImage = new Image(root.getImage(series));
                Pixels exportPixels = new Pixels(root.getImage(series).getPixels());
                exportImage.setPixels(exportPixels);
                OMEXMLMetadataRoot newRoot = (OMEXMLMetadataRoot) meta.getRoot();
                while (newRoot.sizeOfImageList() > 0) {
                    newRoot.removeImage(newRoot.getImage(0));
                }
                newRoot.addImage(exportImage);
                meta.setRoot(newRoot);
                meta.setPixelsSizeX(new PositiveInteger(width), 0);
                meta.setPixelsSizeY(new PositiveInteger(height), 0);
                if (autoscale) {
                    store.setPixelsType(PixelType.UINT8, 0);
                }
                if (channel >= 0) {
                    meta.setPixelsSizeC(new PositiveInteger(1), 0);
                }
                if (zSection >= 0) {
                    meta.setPixelsSizeZ(new PositiveInteger(1), 0);
                }
                if (timepoint >= 0) {
                    meta.setPixelsSizeT(new PositiveInteger(1), 0);
                }
                writer.setMetadataRetrieve((MetadataRetrieve) meta);
            } else {
                for (int i = 0; i < reader.getSeriesCount(); i++) {
                    meta.setPixelsSizeX(new PositiveInteger(width), 0);
                    meta.setPixelsSizeY(new PositiveInteger(height), 0);
                    if (autoscale) {
                        store.setPixelsType(PixelType.UINT8, i);
                    }
                    if (channel >= 0) {
                        meta.setPixelsSizeC(new PositiveInteger(1), 0);
                    }
                    if (zSection >= 0) {
                        meta.setPixelsSizeZ(new PositiveInteger(1), 0);
                    }
                    if (timepoint >= 0) {
                        meta.setPixelsSizeT(new PositiveInteger(1), 0);
                    }
                }
                writer.setMetadataRetrieve((MetadataRetrieve) meta);
            }
        } catch (ServiceException e) {
            throw new FormatException(e);
        }
    }
    writer.setWriteSequentially(true);
    if (writer instanceof TiffWriter) {
        ((TiffWriter) writer).setBigTiff(bigtiff);
    } else if (writer instanceof ImageWriter) {
        IFormatWriter w = ((ImageWriter) writer).getWriter(out);
        if (w instanceof TiffWriter) {
            ((TiffWriter) w).setBigTiff(bigtiff);
        }
    }
    String format = writer.getFormat();
    LOGGER.info("[{}] -> {} [{}]", new Object[] { reader.getFormat(), out, format });
    long mid = System.currentTimeMillis();
    int total = 0;
    int num = writer.canDoStacks() ? reader.getSeriesCount() : 1;
    long read = 0, write = 0;
    int first = series == -1 ? 0 : series;
    int last = series == -1 ? num : series + 1;
    long timeLastLogged = System.currentTimeMillis();
    for (int q = first; q < last; q++) {
        reader.setSeries(q);
        firstTile = true;
        if (!dimensionsSet) {
            width = reader.getSizeX();
            height = reader.getSizeY();
        }
        int writerSeries = series == -1 ? q : 0;
        writer.setSeries(writerSeries);
        writer.setInterleaved(reader.isInterleaved() && !autoscale);
        writer.setValidBitsPerPixel(reader.getBitsPerPixel());
        int numImages = writer.canDoStacks() ? reader.getImageCount() : 1;
        int startPlane = (int) Math.max(0, firstPlane);
        int endPlane = (int) Math.min(numImages, lastPlane);
        numImages = endPlane - startPlane;
        if (channel >= 0) {
            numImages /= reader.getEffectiveSizeC();
        }
        if (zSection >= 0) {
            numImages /= reader.getSizeZ();
        }
        if (timepoint >= 0) {
            numImages /= reader.getSizeT();
        }
        total += numImages;
        int count = 0;
        for (int i = startPlane; i < endPlane; i++) {
            int[] coords = reader.getZCTCoords(i);
            if ((zSection >= 0 && coords[0] != zSection) || (channel >= 0 && coords[1] != channel) || (timepoint >= 0 && coords[2] != timepoint)) {
                continue;
            }
            String outputName = FormatTools.getFilename(q, i, reader, out, zeroPadding);
            if (outputName.equals(FormatTools.getTileFilename(0, 0, 0, outputName))) {
                writer.setId(outputName);
                if (compression != null)
                    writer.setCompression(compression);
            } else {
                int tileNum = outputName.indexOf(FormatTools.TILE_NUM);
                int tileX = outputName.indexOf(FormatTools.TILE_X);
                int tileY = outputName.indexOf(FormatTools.TILE_Y);
                if (tileNum < 0 && (tileX < 0 || tileY < 0)) {
                    throw new FormatException("Invalid file name pattern; " + FormatTools.TILE_NUM + " or both of " + FormatTools.TILE_X + " and " + FormatTools.TILE_Y + " must be specified.");
                }
            }
            int outputIndex = 0;
            if (nextOutputIndex.containsKey(outputName)) {
                outputIndex = nextOutputIndex.get(outputName);
            }
            long s = System.currentTimeMillis();
            long m = convertPlane(writer, i, outputIndex, outputName);
            long e = System.currentTimeMillis();
            read += m - s;
            write += e - m;
            nextOutputIndex.put(outputName, outputIndex + 1);
            if (i == endPlane - 1) {
                nextOutputIndex.remove(outputName);
            }
            // log number of planes processed every second or so
            if (count == numImages - 1 || (e - timeLastLogged) / 1000 > 0) {
                int current = (count - startPlane) + 1;
                int percent = 100 * current / numImages;
                StringBuilder sb = new StringBuilder();
                sb.append("\t");
                int numSeries = last - first;
                if (numSeries > 1) {
                    sb.append("Series ");
                    sb.append(q);
                    sb.append(": converted ");
                } else
                    sb.append("Converted ");
                LOGGER.info(sb.toString() + "{}/{} planes ({}%)", new Object[] { current, numImages, percent });
                timeLastLogged = e;
            }
            count++;
        }
    }
    writer.close();
    long end = System.currentTimeMillis();
    LOGGER.info("[done]");
    // output timing results
    float sec = (end - start) / 1000f;
    long initial = mid - start;
    float readAvg = (float) read / total;
    float writeAvg = (float) write / total;
    LOGGER.info("{}s elapsed ({}+{}ms per plane, {}ms overhead)", new Object[] { sec, readAvg, writeAvg, initial });
    return true;
}
Also used : ServiceFactory(loci.common.services.ServiceFactory) ChannelMerger(loci.formats.ChannelMerger) ImageWriter(loci.formats.ImageWriter) ChannelFiller(loci.formats.ChannelFiller) Image(ome.xml.model.Image) OMEXMLService(loci.formats.services.OMEXMLService) Pixels(ome.xml.model.Pixels) IMetadata(loci.formats.meta.IMetadata) ImageReader(loci.formats.ImageReader) MetadataRetrieve(loci.formats.meta.MetadataRetrieve) PositiveInteger(ome.xml.model.primitives.PositiveInteger) TiffWriter(loci.formats.out.TiffWriter) InputStreamReader(java.io.InputStreamReader) DependencyException(loci.common.services.DependencyException) ChannelSeparator(loci.formats.ChannelSeparator) FormatException(loci.formats.FormatException) MetadataStore(loci.formats.meta.MetadataStore) IFormatWriter(loci.formats.IFormatWriter) FileStitcher(loci.formats.FileStitcher) ServiceException(loci.common.services.ServiceException) OMEXMLMetadataRoot(ome.xml.meta.OMEXMLMetadataRoot) BufferedReader(java.io.BufferedReader) MinMaxCalculator(loci.formats.MinMaxCalculator) MissingLibraryException(loci.formats.MissingLibraryException) Location(loci.common.Location)

Aggregations

ImageReader (loci.formats.ImageReader)71 ServiceFactory (loci.common.services.ServiceFactory)23 OMEXMLService (loci.formats.services.OMEXMLService)23 FormatException (loci.formats.FormatException)20 IFormatReader (loci.formats.IFormatReader)20 IMetadata (loci.formats.meta.IMetadata)19 Test (org.testng.annotations.Test)15 IOException (java.io.IOException)11 ChannelFiller (loci.formats.ChannelFiller)11 DependencyException (loci.common.services.DependencyException)10 ServiceException (loci.common.services.ServiceException)10 ChannelSeparator (loci.formats.ChannelSeparator)10 MinMaxCalculator (loci.formats.MinMaxCalculator)10 BufferedImageReader (loci.formats.gui.BufferedImageReader)8 ImageWriter (loci.formats.ImageWriter)7 File (java.io.File)6 FileStitcher (loci.formats.FileStitcher)6 OMETiffWriter (loci.formats.out.OMETiffWriter)6 Location (loci.common.Location)5 DimensionSwapper (loci.formats.DimensionSwapper)5