use of loci.formats.meta.MetadataStore in project bioformats by openmicroscopy.
the class BurleighReader method initFile.
// -- Internal FormatReader API methods --
/* @see loci.formats.FormatReader#initFile(String) */
@Override
protected void initFile(String id) throws FormatException, IOException {
super.initFile(id);
in = new RandomAccessInputStream(id);
CoreMetadata m = core.get(0);
m.littleEndian = true;
in.order(isLittleEndian());
int version = (int) in.readFloat() - 1;
m.sizeX = in.readShort();
m.sizeY = in.readShort();
double xSize = 0d, ySize = 0d, zSize = 0d;
pixelsOffset = version == 1 ? 8 : 260;
if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) {
double timePerPixel = 0d;
int mode = 0, gain = 0, mag = 0;
double sampleVolts = 0d, tunnelCurrent = 0d;
if (version == 1) {
in.seek(in.length() - 40);
in.skipBytes(12);
xSize = in.readInt();
ySize = in.readInt();
zSize = in.readInt();
timePerPixel = in.readShort() * 50;
mag = in.readShort();
switch(mag) {
case 3:
mag = 10;
break;
case 4:
mag = 50;
break;
case 5:
mag = 250;
break;
}
xSize /= mag;
ySize /= mag;
zSize /= mag;
mode = in.readShort();
gain = in.readShort();
sampleVolts = in.readFloat() / 1000;
tunnelCurrent = in.readFloat();
} else if (version == 2) {
in.skipBytes(14);
xSize = in.readInt();
ySize = in.readInt();
zSize = in.readInt();
mode = in.readShort();
in.skipBytes(4);
gain = in.readShort();
timePerPixel = in.readShort() * 50;
in.skipBytes(12);
sampleVolts = in.readFloat();
tunnelCurrent = in.readFloat();
addGlobalMeta("Force", in.readFloat());
}
addGlobalMeta("Version", version);
addGlobalMeta("Image mode", mode);
addGlobalMeta("Z gain", gain);
addGlobalMeta("Time per pixel (s)", timePerPixel);
addGlobalMeta("Sample volts", sampleVolts);
addGlobalMeta("Tunnel current", tunnelCurrent);
addGlobalMeta("Magnification", mag);
}
m.pixelType = FormatTools.UINT16;
m.sizeZ = 1;
m.sizeC = 1;
m.sizeT = 1;
m.imageCount = 1;
m.dimensionOrder = "XYZCT";
MetadataStore store = makeFilterMetadata();
MetadataTools.populatePixels(store, this);
if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) {
Length sizeX = FormatTools.getPhysicalSizeX(xSize / getSizeX());
Length sizeY = FormatTools.getPhysicalSizeY(ySize / getSizeY());
Length sizeZ = FormatTools.getPhysicalSizeZ(zSize / getSizeZ());
if (sizeX != null) {
store.setPixelsPhysicalSizeX(sizeX, 0);
}
if (sizeY != null) {
store.setPixelsPhysicalSizeY(sizeY, 0);
}
if (sizeZ != null) {
store.setPixelsPhysicalSizeZ(sizeZ, 0);
}
}
}
use of loci.formats.meta.MetadataStore in project bioformats by openmicroscopy.
the class AIMReader method initFile.
// -- Internal FormatReader API methods --
/* @see loci.formats.FormatReader#initFile(String) */
@Override
protected void initFile(String id) throws FormatException, IOException {
super.initFile(id);
in = new RandomAccessInputStream(id);
CoreMetadata m = core.get(0);
m.littleEndian = true;
in.order(isLittleEndian());
// check for newer version of AIM format with wider offsets
String version = in.readString(16);
boolean widerOffsets = version.startsWith("AIMDATA_V030");
if (widerOffsets) {
in.seek(96);
m.sizeX = (int) in.readLong();
m.sizeY = (int) in.readLong();
m.sizeZ = (int) in.readLong();
in.seek(280);
} else {
in.seek(56);
m.sizeX = in.readInt();
m.sizeY = in.readInt();
m.sizeZ = in.readInt();
in.seek(160);
}
m.sizeC = 1;
m.sizeT = 1;
m.imageCount = getSizeZ();
m.pixelType = FormatTools.INT16;
m.dimensionOrder = "XYZCT";
String processingLog = in.readCString();
pixelOffset = in.getFilePointer();
String date = null;
Double xSize = null, xLength = null;
Double ySize = null, yLength = null;
Double zSize = null, zLength = null;
String[] lines = processingLog.split("\n");
for (String line : lines) {
line = line.trim();
int split = line.indexOf(" ");
if (split > 0) {
String key = line.substring(0, split).trim();
String value = line.substring(split).trim();
addGlobalMeta(key, value);
if (key.equals("Original Creation-Date")) {
date = DateTools.formatDate(value, "dd-MMM-yyyy HH:mm:ss", ".");
} else if (key.equals("Orig-ISQ-Dim-p")) {
String[] tokens = value.split(" ");
for (String token : tokens) {
token = token.trim();
if (token.length() > 0) {
if (xSize == null) {
xSize = new Double(token);
} else if (ySize == null) {
ySize = new Double(token);
} else if (zSize == null) {
zSize = new Double(token);
}
}
}
} else if (key.equals("Orig-ISQ-Dim-um")) {
String[] tokens = value.split(" ");
for (String token : tokens) {
token = token.trim();
if (token.length() > 0) {
if (xLength == null) {
xLength = new Double(token);
} else if (yLength == null) {
yLength = new Double(token);
} else if (zLength == null) {
zLength = new Double(token);
}
}
}
}
}
}
MetadataStore store = makeFilterMetadata();
MetadataTools.populatePixels(store, this);
if (date != null) {
store.setImageAcquisitionDate(new Timestamp(date), 0);
}
if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) {
if (xSize != null && xLength != null) {
Double size = xLength / xSize;
Length physicalSize = FormatTools.getPhysicalSizeX(size);
if (physicalSize != null) {
store.setPixelsPhysicalSizeX(physicalSize, 0);
}
}
if (ySize != null && yLength != null) {
Double size = yLength / ySize;
Length physicalSize = FormatTools.getPhysicalSizeY(size);
if (physicalSize != null) {
store.setPixelsPhysicalSizeY(physicalSize, 0);
}
}
if (zSize != null && zLength != null) {
Double size = zLength / zSize;
Length physicalSize = FormatTools.getPhysicalSizeZ(size);
if (physicalSize != null) {
store.setPixelsPhysicalSizeZ(physicalSize, 0);
}
}
}
}
use of loci.formats.meta.MetadataStore in project bioformats by openmicroscopy.
the class ARFReader method initFile.
// -- Internal FormatReader API methods --
/* @see loci.formats.FormatReader#initFile(String) */
@Override
protected void initFile(String id) throws FormatException, IOException {
super.initFile(id);
in = new RandomAccessInputStream(id);
// parse file header
LOGGER.info("Reading file header");
byte endian1 = in.readByte();
byte endian2 = in.readByte();
boolean little;
if (endian1 == 1 && endian2 == 0)
little = true;
else if (endian1 == 0 && endian2 == 1)
little = false;
else
throw new FormatException("Undefined endianness");
in.order(little);
// 'AR' signature
in.skipBytes(2);
int version = in.readUnsignedShort();
int width = in.readUnsignedShort();
int height = in.readUnsignedShort();
int bitsPerPixel = in.readUnsignedShort();
int numImages = version == 2 ? in.readUnsignedShort() : 1;
// NB: The next 510 bytes are unused 'application dependent' data,
// followed by raw image data with no padding.
// populate core metadata
CoreMetadata m = core.get(0);
m.sizeX = width;
m.sizeY = height;
m.sizeZ = 1;
m.sizeC = 1;
m.sizeT = numImages;
int bpp = bitsPerPixel / 8;
if ((bitsPerPixel % 8) != 0)
bpp++;
m.pixelType = FormatTools.pixelTypeFromBytes(bpp, false, false);
m.bitsPerPixel = bitsPerPixel;
m.imageCount = numImages;
m.dimensionOrder = "XYCZT";
m.orderCertain = true;
m.littleEndian = little;
m.rgb = false;
m.interleaved = false;
m.indexed = false;
m.metadataComplete = true;
if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) {
// populate original metadata
addGlobalMeta("Endianness", little ? "little" : "big");
addGlobalMeta("Version", version);
addGlobalMeta("Width", width);
addGlobalMeta("Height", height);
addGlobalMeta("Bits per pixel", bitsPerPixel);
addGlobalMeta("Image count", numImages);
}
// populate OME metadata
MetadataStore store = makeFilterMetadata();
MetadataTools.populatePixels(store, this);
}
use of loci.formats.meta.MetadataStore in project bioformats by openmicroscopy.
the class AliconaReader method initFile.
// -- Internal FormatReader API methods --
/* @see loci.formats.FormatReader#initFile(String) */
@Override
protected void initFile(String id) throws FormatException, IOException {
super.initFile(id);
in = new RandomAccessInputStream(id);
CoreMetadata m = core.get(0);
// check that this is a valid AL3D file
LOGGER.info("Verifying Alicona format");
String magicString = in.readString(17);
if (!magicString.trim().equals("AliconaImaging")) {
throw new FormatException("Invalid magic string : " + "expected 'AliconaImaging', got " + magicString);
}
// now we read a series of tags
// each one is 52 bytes - 20 byte key + 30 byte value + 2 byte CRLF
LOGGER.info("Reading tags");
int count = 2;
boolean hasC = false;
String voltage = null, magnification = null, workingDistance = null;
String pntX = null, pntY = null;
int depthOffset = 0;
for (int i = 0; i < count; i++) {
String key = in.readString(20).trim();
String value = in.readString(30).trim();
addGlobalMeta(key, value);
in.skipBytes(2);
if (key.equals("TagCount"))
count += Integer.parseInt(value);
else if (key.equals("Rows"))
m.sizeY = Integer.parseInt(value);
else if (key.equals("Cols"))
m.sizeX = Integer.parseInt(value);
else if (key.equals("NumberOfPlanes")) {
m.imageCount = Integer.parseInt(value);
} else if (key.equals("TextureImageOffset")) {
textureOffset = Integer.parseInt(value);
} else if (key.equals("TexturePtr") && !value.equals("7"))
hasC = true;
else if (key.equals("Voltage"))
voltage = value;
else if (key.equals("Magnification"))
magnification = value;
else if (key.equals("PixelSizeXMeter"))
pntX = value;
else if (key.equals("PixelSizeYMeter"))
pntY = value;
else if (key.equals("WorkingDistance"))
workingDistance = value;
else if (key.equals("DepthImageOffset")) {
depthOffset = Integer.parseInt(value);
}
}
LOGGER.info("Populating metadata");
if (textureOffset != 0) {
numBytes = (int) (in.length() - textureOffset) / (getSizeX() * getSizeY() * getImageCount());
m.sizeC = hasC ? 3 : 1;
m.sizeZ = 1;
m.sizeT = getImageCount() / getSizeC();
m.pixelType = FormatTools.pixelTypeFromBytes(numBytes, false, false);
} else {
textureOffset = depthOffset;
m.pixelType = FormatTools.FLOAT;
m.sizeC = 1;
m.sizeZ = 1;
m.sizeT = 1;
m.imageCount = 1;
}
m.rgb = false;
m.interleaved = false;
m.littleEndian = true;
m.dimensionOrder = "XYCTZ";
m.metadataComplete = true;
m.indexed = false;
m.falseColor = false;
MetadataStore store = makeFilterMetadata();
MetadataTools.populatePixels(store, this);
if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) {
// link Image and Instrument
String instrumentID = MetadataTools.createLSID("Instrument", 0);
store.setInstrumentID(instrumentID, 0);
store.setImageInstrumentRef(instrumentID, 0);
// used when the dataset was acquired, i.e. detector settings.
if (voltage != null) {
store.setDetectorSettingsVoltage(new ElectricPotential(new Double(voltage), UNITS.VOLT), 0, 0);
// link DetectorSettings to an actual Detector
String detectorID = MetadataTools.createLSID("Detector", 0, 0);
store.setDetectorID(detectorID, 0, 0);
store.setDetectorSettingsID(detectorID, 0, 0);
// set required Detector type
store.setDetectorType(getDetectorType("Other"), 0, 0);
}
if (magnification != null) {
store.setObjectiveCalibratedMagnification(new Double(magnification), 0, 0);
}
if (workingDistance != null) {
store.setObjectiveWorkingDistance(new Length(new Double(workingDistance), UNITS.MICROMETER), 0, 0);
}
store.setObjectiveCorrection(getCorrection("Other"), 0, 0);
store.setObjectiveImmersion(getImmersion("Other"), 0, 0);
// link Objective to an Image using ObjectiveSettings
String objectiveID = MetadataTools.createLSID("Objective", 0, 0);
store.setObjectiveID(objectiveID, 0, 0);
store.setObjectiveSettingsID(objectiveID, 0);
if (pntX != null && pntY != null) {
double pixelSizeX = Double.parseDouble(pntX);
double pixelSizeY = Double.parseDouble(pntY);
Length sizeX = FormatTools.getPhysicalSizeX(pixelSizeX, UNITS.METER);
Length sizeY = FormatTools.getPhysicalSizeY(pixelSizeY, UNITS.METER);
if (sizeX != null) {
store.setPixelsPhysicalSizeX(sizeX, 0);
}
if (sizeY != null) {
store.setPixelsPhysicalSizeY(sizeY, 0);
}
}
}
}
use of loci.formats.meta.MetadataStore in project bioformats by openmicroscopy.
the class AnalyzeReader method initFile.
// -- Internal FormatReader API methods --
/* @see loci.formats.FormatReader#initFile(String) */
@Override
protected void initFile(String id) throws FormatException, IOException {
// the dataset has two files - we want the one ending in '.hdr'
if (id.endsWith(".img")) {
LOGGER.info("Looking for header file");
String header = id.substring(0, id.lastIndexOf(".")) + ".hdr";
if (new Location(header).exists()) {
setId(header);
return;
} else
throw new FormatException("Header file not found.");
}
super.initFile(id);
in = new RandomAccessInputStream(id);
pixelsFilename = id.substring(0, id.lastIndexOf(".")) + ".img";
pixelFile = new RandomAccessInputStream(pixelsFilename);
LOGGER.info("Reading header");
int fileSize = in.readInt();
boolean little = fileSize != in.length();
in.order(little);
pixelFile.order(little);
in.skipBytes(10);
String imageName = in.readString(18);
in.skipBytes(8);
int ndims = in.readShort();
int x = in.readShort();
int y = in.readShort();
int z = in.readShort();
int t = in.readShort();
in.skipBytes(20);
int dataType = in.readShort();
int nBitsPerPixel = in.readShort();
String description = null;
double voxelWidth = 0d, voxelHeight = 0d, sliceThickness = 0d, deltaT = 0d;
if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) {
in.skipBytes(6);
voxelWidth = in.readFloat();
voxelHeight = in.readFloat();
sliceThickness = in.readFloat();
deltaT = in.readFloat();
in.skipBytes(12);
pixelOffset = (int) in.readFloat();
in.skipBytes(12);
float calibratedMax = in.readFloat();
float calibratedMin = in.readFloat();
float compressed = in.readFloat();
float verified = in.readFloat();
float pixelMax = in.readFloat();
float pixelMin = in.readFloat();
description = in.readString(80);
String auxFile = in.readString(24);
char orient = (char) in.readByte();
String originator = in.readString(10);
String generated = in.readString(10);
String scannum = in.readString(10);
String patientID = in.readString(10);
String expDate = in.readString(10);
String expTime = in.readString(10);
in.skipBytes(3);
int views = in.readInt();
int volsAdded = in.readInt();
int startField = in.readInt();
int fieldSkip = in.readInt();
int omax = in.readInt();
int omin = in.readInt();
int smax = in.readInt();
int smin = in.readInt();
addGlobalMeta("Database name", imageName);
addGlobalMeta("Number of dimensions", ndims);
addGlobalMeta("Data type", dataType);
addGlobalMeta("Number of bits per pixel", nBitsPerPixel);
addGlobalMeta("Voxel width", voxelWidth);
addGlobalMeta("Voxel height", voxelHeight);
addGlobalMeta("Slice thickness", sliceThickness);
addGlobalMeta("Exposure time", deltaT);
addGlobalMeta("Pixel offset", pixelOffset);
addGlobalMeta("Calibrated maximum", calibratedMax);
addGlobalMeta("Calibrated minimum", calibratedMin);
addGlobalMeta("Compressed", compressed);
addGlobalMeta("Verified", verified);
addGlobalMeta("Pixel maximum", pixelMax);
addGlobalMeta("Pixel minimum", pixelMin);
addGlobalMeta("Description", description);
addGlobalMeta("Auxiliary file", auxFile);
addGlobalMeta("Orientation", orient);
addGlobalMeta("Originator", originator);
addGlobalMeta("Generated", generated);
addGlobalMeta("Scan Number", scannum);
addGlobalMeta("Patient ID", patientID);
addGlobalMeta("Acquisition Date", expDate);
addGlobalMeta("Acquisition Time", expTime);
} else {
in.skipBytes(34);
pixelOffset = (int) in.readFloat();
}
LOGGER.info("Populating core metadata");
CoreMetadata m = core.get(0);
m.littleEndian = little;
m.sizeX = x;
m.sizeY = y;
m.sizeZ = z;
m.sizeT = t;
m.sizeC = 1;
if (getSizeZ() == 0)
m.sizeZ = 1;
if (getSizeT() == 0)
m.sizeT = 1;
m.imageCount = getSizeZ() * getSizeT();
m.rgb = false;
m.interleaved = false;
m.indexed = false;
m.dimensionOrder = "XYZTC";
switch(dataType) {
case 1:
case 2:
m.pixelType = FormatTools.UINT8;
break;
case 4:
m.pixelType = FormatTools.INT16;
break;
case 8:
m.pixelType = FormatTools.INT32;
break;
case 16:
m.pixelType = FormatTools.FLOAT;
break;
case 64:
m.pixelType = FormatTools.DOUBLE;
break;
case 128:
m.pixelType = FormatTools.UINT8;
m.sizeC = 3;
m.rgb = true;
m.interleaved = true;
m.dimensionOrder = "XYCZT";
default:
throw new FormatException("Unsupported data type: " + dataType);
}
LOGGER.info("Populating MetadataStore");
MetadataStore store = makeFilterMetadata();
MetadataTools.populatePixels(store, this);
store.setImageName(imageName, 0);
if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) {
store.setImageDescription(description, 0);
Length sizeX = FormatTools.getPhysicalSizeX(voxelWidth, UNITS.MILLIMETER);
Length sizeY = FormatTools.getPhysicalSizeY(voxelHeight, UNITS.MILLIMETER);
Length sizeZ = FormatTools.getPhysicalSizeZ(sliceThickness, UNITS.MILLIMETER);
if (sizeX != null) {
store.setPixelsPhysicalSizeX(sizeX, 0);
}
if (sizeY != null) {
store.setPixelsPhysicalSizeY(sizeY, 0);
}
if (sizeZ != null) {
store.setPixelsPhysicalSizeZ(sizeZ, 0);
}
store.setPixelsTimeIncrement(new Time(deltaT, UNITS.MILLISECOND), 0);
}
}
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