use of ome.xml.model.enums.Binning in project bioformats by openmicroscopy.
the class ZeissCZIReader method translateExperiment.
private void translateExperiment(Element root) throws FormatException {
NodeList experiments = root.getElementsByTagName("Experiment");
if (experiments == null || experiments.getLength() == 0) {
return;
}
Element experimentBlock = getFirstNode((Element) experiments.item(0), "ExperimentBlocks");
Element acquisition = getFirstNode(experimentBlock, "AcquisitionBlock");
Element tilesSetup = getFirstNode(acquisition, "TilesSetup");
NodeList groups = getGrandchildren(tilesSetup, "PositionGroup");
positionsX = new Length[core.size()];
positionsY = new Length[core.size()];
positionsZ = new Length[core.size()];
if (groups != null) {
for (int i = 0; i < groups.getLength(); i++) {
Element group = (Element) groups.item(i);
Element position = getFirstNode(group, "Position");
String tilesXValue = getFirstNodeValue(group, "TilesX");
String tilesYValue = getFirstNodeValue(group, "TilesY");
if (position != null && tilesXValue != null && !tilesXValue.isEmpty() && tilesYValue != null && !tilesYValue.isEmpty()) {
Integer tilesX = DataTools.parseInteger(tilesXValue);
Integer tilesY = DataTools.parseInteger(tilesYValue);
String x = position.getAttribute("X");
String y = position.getAttribute("Y");
String z = position.getAttribute("Z");
Length xPos = null;
try {
xPos = new Length(Double.valueOf(x), UNITS.METRE);
} catch (NumberFormatException e) {
}
Length yPos = null;
try {
yPos = new Length(Double.valueOf(y), UNITS.METRE);
} catch (NumberFormatException e) {
}
Length zPos = null;
try {
zPos = new Length(Double.valueOf(z), UNITS.METRE);
} catch (NumberFormatException e) {
}
int numTiles = (tilesX == null || tilesY == null) ? 0 : tilesX * tilesY;
for (int tile = 0; tile < numTiles; tile++) {
int index = i * tilesX * tilesY + tile;
if (groups.getLength() == core.size()) {
index = i;
}
if (index < positionsX.length) {
positionsX[index] = xPos;
positionsY[index] = yPos;
positionsZ[index] = zPos;
}
}
}
}
} else {
Element regionsSetup = getFirstNode(acquisition, "RegionsSetup");
if (regionsSetup != null) {
Element sampleHolder = getFirstNode(regionsSetup, "SampleHolder");
if (sampleHolder != null) {
NodeList regions = getGrandchildren(sampleHolder, "SingleTileRegionArray", "SingleTileRegion");
if (regions != null) {
for (int i = 0; i < regions.getLength(); i++) {
Element region = (Element) regions.item(i);
String x = getFirstNode(region, "X").getTextContent();
String y = getFirstNode(region, "Y").getTextContent();
String z = getFirstNode(region, "Z").getTextContent();
// safe to assume all 3 arrays have the same length
if (i < positionsX.length) {
if (x == null) {
positionsX[i] = null;
} else {
final Double number = Double.valueOf(x);
positionsX[i] = new Length(number, UNITS.MICROMETER);
}
if (y == null) {
positionsY[i] = null;
} else {
final Double number = Double.valueOf(y);
positionsY[i] = new Length(number, UNITS.MICROMETER);
}
if (z == null) {
positionsZ[i] = null;
} else {
final Double number = Double.valueOf(z);
positionsZ[i] = new Length(number, UNITS.MICROMETER);
}
}
}
}
}
}
}
NodeList detectors = getGrandchildren(acquisition, "Detector");
Element setup = getFirstNode(acquisition, "AcquisitionModeSetup");
String cameraModel = getFirstNodeValue(setup, "SelectedCamera");
if (detectors != null) {
for (int i = 0; i < detectors.getLength(); i++) {
Element detector = (Element) detectors.item(i);
String id = MetadataTools.createLSID("Detector", 0, i);
store.setDetectorID(id, 0, i);
String model = detector.getAttribute("Id");
store.setDetectorModel(model, 0, i);
String bin = getFirstNodeValue(detector, "Binning");
if (bin != null) {
bin = bin.replaceAll(",", "x");
Binning binning = getBinning(bin);
if (model != null && model.equals(cameraModel)) {
for (int image = 0; image < getSeriesCount(); image++) {
for (int c = 0; c < getEffectiveSizeC(); c++) {
store.setDetectorSettingsID(id, image, c);
store.setDetectorSettingsBinning(binning, image, c);
}
}
hasDetectorSettings = true;
}
}
}
}
Element multiTrack = getFirstNode(acquisition, "MultiTrackSetup");
if (multiTrack == null) {
return;
}
NodeList detectorGroups = multiTrack.getElementsByTagName("Detectors");
for (int d = 0; d < detectorGroups.getLength(); d++) {
Element detectorGroup = (Element) detectorGroups.item(d);
detectors = detectorGroup.getElementsByTagName("Detector");
if (detectors != null && detectors.getLength() > 0) {
for (int i = 0; i < detectors.getLength(); i++) {
Element detector = (Element) detectors.item(i);
String voltage = getFirstNodeValue(detector, "Voltage");
if (i == 0 && d == 0) {
gain = voltage;
}
gains.add(voltage);
}
}
}
NodeList tracks = multiTrack.getElementsByTagName("Track");
if (tracks != null && tracks.getLength() > 0) {
for (int i = 0; i < tracks.getLength(); i++) {
Element track = (Element) tracks.item(i);
Element channel = getFirstNode(track, "Channel");
String exposure = getFirstNodeValue(channel, "ExposureTime");
String gain = getFirstNodeValue(channel, "EMGain");
while (channels.size() <= i) {
channels.add(new Channel());
}
try {
if (exposure != null) {
channels.get(i).exposure = new Double(exposure);
}
} catch (NumberFormatException e) {
LOGGER.debug("Could not parse exposure time", e);
}
try {
if (gain != null) {
channels.get(i).gain = new Double(gain);
}
} catch (NumberFormatException e) {
LOGGER.debug("Could not parse gain", e);
}
}
}
}
use of ome.xml.model.enums.Binning in project bioformats by openmicroscopy.
the class PCIReader method initFile.
// -- Internal FormatReader API methods --
/* @see loci.formats.FormatReader#initFile(String) */
@Override
protected void initFile(String id) throws FormatException, IOException {
super.initFile(id);
imageFiles = new HashMap<Integer, String>();
timestamps = new HashMap<Integer, Double>();
uniqueZ = new ArrayList<Double>();
CoreMetadata m = core.get(0);
initPOIService();
double scaleFactor = 1;
final List<String> allFiles = poi.getDocumentList();
if (allFiles.isEmpty()) {
throw new FormatException("No files were found - the .cxd may be corrupt.");
}
double firstZ = 0d, secondZ = 0d;
int mode = 0;
for (String name : allFiles) {
int separator = name.lastIndexOf(File.separator);
String parent = name.substring(0, separator);
String relativePath = name.substring(separator + 1);
RandomAccessInputStream stream = null;
if (!(relativePath.startsWith("Bitmap") || (relativePath.equals("Data") && parent.indexOf("Image") != -1))) {
stream = poi.getDocumentStream(name);
stream.order(true);
}
if (stream != null && stream.length() == 8) {
double value = stream.readDouble();
stream.seek(0);
String key = name.replace(File.separatorChar, ' ');
key = key.replaceAll("Root Entry ", "");
key = key.replaceAll("Field Data ", "");
key = key.replaceAll("Details ", "");
addGlobalMeta(key, value);
}
if (relativePath.equals("Field Count")) {
m.imageCount = stream.readInt();
} else if (relativePath.equals("File Has Image")) {
if (stream.readShort() == 0) {
throw new FormatException("This file does not contain image data.");
}
} else if (relativePath.startsWith("Bitmap") || (relativePath.equals("Data") && parent.indexOf("Image") != -1)) {
imageFiles.put(imageFiles.size(), name);
if (getSizeX() != 0 && getSizeY() != 0) {
int bpp = FormatTools.getBytesPerPixel(getPixelType());
int plane = getSizeX() * getSizeY() * bpp;
if (getSizeC() == 0 || getSizeC() * plane > poi.getFileSize(name)) {
m.sizeC = poi.getFileSize(name) / plane;
}
}
} else if (relativePath.indexOf("Image_Depth") != -1) {
boolean firstBits = m.bitsPerPixel == 0;
int bits = (int) stream.readDouble();
m.bitsPerPixel = bits;
while (bits % 8 != 0 || bits == 0) bits++;
if (bits % 3 == 0) {
m.sizeC = 3;
bits /= 3;
m.bitsPerPixel /= 3;
}
bits /= 8;
m.pixelType = FormatTools.pixelTypeFromBytes(bits, false, false);
if (getSizeC() > 1 && firstBits) {
m.sizeC /= bits;
}
} else if (relativePath.indexOf("Image_Height") != -1 && getSizeY() == 0) {
m.sizeY = (int) stream.readDouble();
} else if (relativePath.indexOf("Image_Width") != -1 && getSizeX() == 0) {
m.sizeX = (int) stream.readDouble();
} else if (relativePath.indexOf("Time_From_Start") != -1) {
timestamps.put(getTimestampIndex(parent), stream.readDouble());
} else if (relativePath.indexOf("Position_Z") != -1) {
double zPos = stream.readDouble();
if (!uniqueZ.contains(zPos) && getSizeZ() <= 1) {
uniqueZ.add(zPos);
}
if (name.indexOf("Field 1" + File.separator) != -1)
firstZ = zPos;
else if (name.indexOf("Field 2" + File.separator) != -1)
secondZ = zPos;
} else if (relativePath.equals("First Field Date & Time")) {
long date = (long) stream.readDouble() * 1000;
creationDate = DateTools.convertDate(date, DateTools.COBOL);
} else if (relativePath.equals("GroupMode")) {
mode = stream.readInt();
} else if (relativePath.equals("GroupSelectedFields")) {
m.sizeZ = (int) (stream.length() / 8);
} else if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) {
if (relativePath.equals("Binning")) {
binning = (int) stream.readDouble();
} else if (relativePath.equals("Comments")) {
String comments = stream.readString((int) stream.length());
String[] lines = comments.split("\n");
for (String line : lines) {
int eq = line.indexOf('=');
if (eq != -1) {
String key = line.substring(0, eq).trim();
String value = line.substring(eq + 1).trim();
addGlobalMeta(key, value);
if (key.equals("factor")) {
if (value.indexOf(';') != -1) {
value = value.substring(0, value.indexOf(';'));
}
scaleFactor = Double.parseDouble(value.trim());
}
}
}
}
}
if (stream != null) {
stream.close();
}
}
boolean zFirst = Math.abs(firstZ - secondZ) > Constants.EPSILON;
if (getSizeC() == 0)
m.sizeC = 1;
if (mode == 0) {
m.sizeZ = 0;
}
if (getSizeZ() <= 1 || (getImageCount() % getSizeZ()) != 0) {
m.sizeZ = uniqueZ.isEmpty() ? 1 : uniqueZ.size();
}
m.sizeT = getImageCount() / getSizeZ();
while (getSizeZ() * getSizeT() < getImageCount()) {
m.sizeZ++;
m.sizeT = getImageCount() / getSizeZ();
}
m.rgb = getSizeC() > 1;
if (imageFiles.size() > getImageCount() && getSizeC() == 1) {
m.sizeC = imageFiles.size() / getImageCount();
m.imageCount *= getSizeC();
} else {
m.imageCount = getSizeZ() * getSizeT();
}
m.interleaved = false;
m.dimensionOrder = zFirst ? "XYCZT" : "XYCTZ";
m.littleEndian = true;
m.indexed = false;
m.falseColor = false;
m.metadataComplete = true;
// re-index image files
String[] files = imageFiles.values().toArray(new String[imageFiles.size()]);
for (String file : files) {
int separator = file.lastIndexOf(File.separator);
String parent = file.substring(0, separator);
imageFiles.put(getImageIndex(parent), file);
}
int bpp = FormatTools.getBytesPerPixel(m.pixelType);
int expectedPlaneSize = m.sizeX * m.sizeY * bpp * m.sizeC;
String file = imageFiles.get(0);
RandomAccessInputStream s = poi.getDocumentStream(file);
TiffParser tp = new TiffParser(s);
// don't correct the image width if it's stored as a TIFF
if (!tp.isValidHeader() && s.length() > expectedPlaneSize) {
m.sizeX += (s.length() - expectedPlaneSize) / (m.sizeY * bpp * m.sizeC);
}
s.close();
MetadataStore store = makeFilterMetadata();
MetadataTools.populatePixels(store, this, true);
if (creationDate != null) {
store.setImageAcquisitionDate(new Timestamp(creationDate), 0);
}
if (getMetadataOptions().getMetadataLevel() != MetadataLevel.MINIMUM) {
Length sizeX = FormatTools.getPhysicalSizeX(scaleFactor);
Length sizeY = FormatTools.getPhysicalSizeY(scaleFactor);
if (sizeX != null) {
store.setPixelsPhysicalSizeX(sizeX, 0);
}
if (sizeY != null) {
store.setPixelsPhysicalSizeY(sizeY, 0);
}
for (int i = 0; i < timestamps.size(); i++) {
if (i >= getImageCount()) {
break;
}
Double timestamp = timestamps.get(i);
if (timestamp != null) {
store.setPlaneDeltaT(new Time(timestamp, UNITS.SECOND), 0, i);
}
if (i == 2) {
Double first = timestamps.get(1);
Double increment = timestamp - first;
if (increment != null) {
store.setPixelsTimeIncrement(new Time(increment, UNITS.SECOND), 0);
}
}
}
if (binning > 0) {
String instrumentID = MetadataTools.createLSID("Instrument", 0);
String detectorID = MetadataTools.createLSID("Detector", 0);
store.setInstrumentID(instrumentID, 0);
store.setDetectorID(detectorID, 0, 0);
store.setDetectorType(getDetectorType("Other"), 0, 0);
store.setImageInstrumentRef(instrumentID, 0);
Binning binningEnum = getBinning(binning + "x" + binning);
for (int c = 0; c < getEffectiveSizeC(); c++) {
store.setDetectorSettingsID(detectorID, 0, c);
store.setDetectorSettingsBinning(binningEnum, 0, c);
}
}
}
}
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