use of org.biojava.bio.symbol.IllegalSymbolException in project ice by JBEI.
the class SequenceUtils method reverseComplement.
/**
* Calculate the reverse complement of the given DNA sequence.
*
* @param sequence DNA sequence to reverse complement.
* @return Reversed, complemented sequence.
* @throws UtilityException
*/
public static String reverseComplement(String sequence) throws UtilityException {
SymbolList symL;
try {
symL = DNATools.createDNA(sequence);
symL = DNATools.reverseComplement(symL);
} catch (IllegalSymbolException | IllegalAlphabetException e) {
throw new UtilityException(e);
}
return symL.seqString();
}
use of org.biojava.bio.symbol.IllegalSymbolException in project ice by JBEI.
the class BlastPlus method writeBigFastaFile.
/**
* Retrieve all the sequences from the database, and writes it out to a fasta file on disk.
*
* @throws BlastException
*/
private static void writeBigFastaFile(BufferedWriter writer) throws BlastException {
SequenceDAO sequenceDAO = DAOFactory.getSequenceDAO();
long count = sequenceDAO.getSequenceCount();
if (count <= 0)
return;
int offset = 0;
while (offset < count) {
Sequence sequence = sequenceDAO.getSequence(offset++);
if (sequence == null || sequence.getEntry() == null)
continue;
long id = sequence.getEntry().getId();
String sequenceString = "";
String temp = sequence.getSequence();
// int sequenceLength = 0;
if (temp != null) {
SymbolList symL;
try {
symL = DNATools.createDNA(sequence.getSequence().trim());
} catch (IllegalSymbolException e1) {
// maybe it's rna?
try {
symL = RNATools.createRNA(sequence.getSequence().trim());
} catch (IllegalSymbolException e2) {
// skip this sequence
Logger.debug("Invalid characters in sequence for " + sequence.getEntry().getId() + ". Skipped for indexing");
Logger.debug(e2.toString());
continue;
}
}
// sequenceLength = symL.seqString().length();
sequenceString = SequenceUtils.breakUpLines(symL.seqString() + symL.seqString());
}
if (sequenceString.length() > 0) {
try {
String idString = ">" + id;
idString += DELIMITER + sequence.getEntry().getRecordType();
String name = sequence.getEntry().getName() == null ? "None" : sequence.getEntry().getName();
idString += DELIMITER + name;
String pNumber = sequence.getEntry().getPartNumber();
idString += DELIMITER + pNumber;
idString += "\n";
writer.write(idString);
writer.write(sequenceString + "\n");
} catch (IOException e) {
throw new BlastException(e);
}
}
}
}
use of org.biojava.bio.symbol.IllegalSymbolException in project ice by JBEI.
the class BlastPlus method writeBigFastaFileForFeatures.
/**
* Writes the fasta file (part of the blast database) that contains all the features that exists on this system.
* This routine is expected to be called as part of the blast sequence feature database rebuild
*
* @param writer writer for fasta file
* @throws BlastException
*/
private static void writeBigFastaFileForFeatures(BufferedWriter writer) throws BlastException {
FeatureDAO featureDAO = DAOFactory.getFeatureDAO();
SequenceFeatureDAO sequenceFeatureDAO = DAOFactory.getSequenceFeatureDAO();
long count = featureDAO.getFeatureCount(null);
if (count <= 0)
return;
int offset = 0;
while (offset < count) {
List<Feature> features = featureDAO.getFeatures(offset++, 1, null);
Feature feature = features.get(0);
String featureName = feature.getName();
if (featureName == null || featureName.trim().isEmpty())
continue;
if (feature.getCuration() != null && feature.getCuration().isExclude())
continue;
boolean hasNegativeStrand = false;
boolean hasPositiveStrand = false;
List<SequenceFeature> sequenceFeatures = sequenceFeatureDAO.getByFeature(feature);
if (sequenceFeatures == null || sequenceFeatures.isEmpty()) {
hasPositiveStrand = true;
} else {
for (SequenceFeature sequenceFeature : sequenceFeatures) {
if (sequenceFeature.getStrand() == 1) {
hasPositiveStrand = true;
} else if (sequenceFeature.getStrand() == -1) {
hasNegativeStrand = true;
}
}
}
try {
String sequenceString = feature.getSequence().trim();
if (StringUtils.isEmpty(sequenceString))
continue;
if (hasNegativeStrand) {
try {
SymbolList symbolList = DNATools.createDNA(sequenceString);
symbolList = DNATools.reverseComplement(symbolList);
writeSequenceString(feature, writer, symbolList.seqString(), -1);
} catch (IllegalSymbolException | IllegalAlphabetException e) {
Logger.warn(e.getMessage());
continue;
}
}
if (hasPositiveStrand) {
writeSequenceString(feature, writer, sequenceString, 1);
}
} catch (IOException e) {
throw new BlastException(e);
}
}
}
use of org.biojava.bio.symbol.IllegalSymbolException in project ice by JBEI.
the class SequenceUtils method translateToProtein.
/**
* Calculate the amino acid translation of the given dnaSequence string.
*
* @param dnaSequence DNA sequence to translate.
* @return String of amino acid symbols.
* @throws UtilityException
*/
public static String translateToProtein(String dnaSequence) throws UtilityException {
SymbolList symL = null;
try {
symL = DNATools.createDNA(dnaSequence);
symL = DNATools.toProtein(symL);
} catch (IllegalSymbolException | IllegalAlphabetException e) {
throw new UtilityException(e);
}
return symL.seqString();
}
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