Examples with Haplotype org.broadinstitute.hellbender.utils.haplotype.Haplotype used on opensource projects

Example 81 with Haplotype

use of org.broadinstitute.hellbender.utils.haplotype.Haplotype in project gatk by broadinstitute.

the class VectorLoglessPairHMM method initialize.

/**
     * {@inheritDoc}
     */
@Override
public void initialize(final List<Haplotype> haplotypes, final Map<String, List<GATKRead>> perSampleReadList, final int readMaxLength, final int haplotypeMaxLength) {
    // do not need to call super.initialize()
    int numHaplotypes = haplotypes.size();
    mHaplotypeDataArray = new HaplotypeDataHolder[numHaplotypes];
    int idx = 0;
    haplotypeToHaplotypeListIdxMap.clear();
    for (final Haplotype currHaplotype : haplotypes) {
        mHaplotypeDataArray[idx] = new HaplotypeDataHolder();
        mHaplotypeDataArray[idx].haplotypeBases = currHaplotype.getBases();
        haplotypeToHaplotypeListIdxMap.put(currHaplotype, idx);
        ++idx;
    }
}

Example 82 with Haplotype

use of org.broadinstitute.hellbender.utils.haplotype.Haplotype in project gatk by broadinstitute.

the class PairHMMLikelihoodCalculationEngineUnitTest method testComputeLikelihoods.

@Test
public void testComputeLikelihoods() {
    final LikelihoodEngineArgumentCollection LEAC = new LikelihoodEngineArgumentCollection();
    PairHMMLikelihoodCalculationEngine.writeLikelihoodsToFile = true;
    final ReadLikelihoodCalculationEngine lce = new PairHMMLikelihoodCalculationEngine((byte) SAMUtils.MAX_PHRED_SCORE, new PairHMMNativeArguments(), PairHMM.Implementation.LOGLESS_CACHING, MathUtils.logToLog10(QualityUtils.qualToErrorProbLog10(LEAC.phredScaledGlobalReadMismappingRate)), PairHMMLikelihoodCalculationEngine.PCRErrorModel.CONSERVATIVE);
    final Map<String, List<GATKRead>> perSampleReadList = new HashMap<>();
    final int n = 10;
    final GATKRead read1 = ArtificialReadUtils.createArtificialRead(TextCigarCodec.decode(n + "M"));
    read1.setMappingQuality(60);
    final String sample1 = "sample1";
    perSampleReadList.put(sample1, Arrays.asList(read1));
    final SampleList samples = new IndexedSampleList(sample1);
    final AssemblyResultSet assemblyResultSet = new AssemblyResultSet();
    final byte[] bases = Strings.repeat("A", n + 1).getBytes();
    final Haplotype hap1 = new Haplotype(bases, true);
    hap1.setGenomeLocation(read1);
    assemblyResultSet.add(hap1);
    final byte[] basesModified = bases;
    //different bases
    basesModified[5] = 'C';
    final Haplotype hap2 = new Haplotype(basesModified, false);
    //use same loc
    hap2.setGenomeLocation(read1);
    assemblyResultSet.add(hap2);
    final ReadLikelihoods<Haplotype> likes = lce.computeReadLikelihoods(assemblyResultSet, samples, perSampleReadList);
    final LikelihoodMatrix<Haplotype> mtx = likes.sampleMatrix(0);
    Assert.assertEquals(mtx.numberOfAlleles(), 2);
    Assert.assertEquals(mtx.numberOfReads(), 1);
    final double v1 = mtx.get(0, 0);
    final double v2 = mtx.get(1, 0);
    Assert.assertTrue(v1 > v2, "matching haplotype should have a higher likelihood");
    lce.close();
    new File(PairHMMLikelihoodCalculationEngine.LIKELIHOODS_FILENAME).delete();
}

Example 83 with Haplotype

use of org.broadinstitute.hellbender.utils.haplotype.Haplotype in project gatk by broadinstitute.

the class RandomLikelihoodCalculationEngineUnitTest method testComputeLikelihoods.

@Test
public void testComputeLikelihoods() {
    final ReadLikelihoodCalculationEngine lce = new RandomLikelihoodCalculationEngine();
    final Map<String, List<GATKRead>> perSampleReadList = new HashMap<>();
    final int n = 10;
    final GATKRead read1 = ArtificialReadUtils.createArtificialRead(TextCigarCodec.decode(n + "M"));
    read1.setMappingQuality(60);
    final String sample1 = "sample1";
    perSampleReadList.put(sample1, Arrays.asList(read1));
    final SampleList samples = new IndexedSampleList(sample1);
    final AssemblyResultSet assemblyResultSet = new AssemblyResultSet();
    final byte[] bases = Strings.repeat("A", n + 1).getBytes();
    final Haplotype hap1 = new Haplotype(bases, true);
    hap1.setGenomeLocation(read1);
    assemblyResultSet.add(hap1);
    final byte[] basesModified = bases;
    //different bases
    basesModified[5] = 'C';
    final Haplotype hap2 = new Haplotype(basesModified, false);
    //use same loc
    hap2.setGenomeLocation(read1);
    assemblyResultSet.add(hap2);
    final ReadLikelihoods<Haplotype> likes = lce.computeReadLikelihoods(assemblyResultSet, samples, perSampleReadList);
    final LikelihoodMatrix<Haplotype> mtx = likes.sampleMatrix(0);
    Assert.assertEquals(mtx.numberOfAlleles(), 2);
    Assert.assertEquals(mtx.numberOfReads(), 1);
    final double v1 = mtx.get(0, 0);
    final double v2 = mtx.get(1, 0);
    Assert.assertTrue(v1 < 0);
    Assert.assertTrue(v2 < 0);
    lce.close();
}

Example 84 with Haplotype

use of org.broadinstitute.hellbender.utils.haplotype.Haplotype in project gatk by broadinstitute.

the class ReferenceConfidenceModelUnitTest method testRefConfidenceBasic.

@Test(dataProvider = "RefConfidenceData")
public void testRefConfidenceBasic(final int nReads, final int extension) {
    final RefConfData data = new RefConfData("ACGTAACCGGTT", extension);
    final List<Haplotype> haplotypes = Arrays.asList(data.getRefHap());
    final List<VariantContext> calls = Collections.emptyList();
    for (int i = 0; i < nReads; i++) {
        data.getActiveRegion().add(data.makeRead(0, data.getRefLength()));
    }
    final ReadLikelihoods<Haplotype> likelihoods = createDummyStratifiedReadMap(data.getRefHap(), samples, data.getActiveRegion());
    final PloidyModel ploidyModel = new HomogeneousPloidyModel(samples, 2);
    final IndependentSampleGenotypesModel genotypingModel = new IndependentSampleGenotypesModel();
    final List<Integer> expectedDPs = Collections.nCopies(data.getActiveRegion().getSpan().size(), nReads);
    final List<VariantContext> contexts = model.calculateRefConfidence(data.getRefHap(), haplotypes, data.getPaddedRefLoc(), data.getActiveRegion(), likelihoods, ploidyModel, calls);
    checkReferenceModelResult(data, contexts, expectedDPs, calls);
}

Example 85 with Haplotype

use of org.broadinstitute.hellbender.utils.haplotype.Haplotype in project gatk by broadinstitute.

the class ReadThreadingAssemblerUnitTest method assemble.

private List<Haplotype> assemble(final ReadThreadingAssembler assembler, final byte[] refBases, final SimpleInterval loc, final List<GATKRead> reads) {
    final Haplotype refHaplotype = new Haplotype(refBases, true);
    final Cigar c = new Cigar();
    c.add(new CigarElement(refHaplotype.getBases().length, CigarOperator.M));
    refHaplotype.setCigar(c);
    final AssemblyRegion activeRegion = new AssemblyRegion(loc, null, true, 0, header);
    activeRegion.addAll(reads);
    //        logger.warn("Assembling " + activeRegion + " with " + engine);
    final AssemblyResultSet assemblyResultSet = assembler.runLocalAssembly(activeRegion, refHaplotype, refBases, loc, Collections.<VariantContext>emptyList(), null, header);
    return assemblyResultSet.getHaplotypeList();
}