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Example 56 with Origin

use of org.vcell.util.Origin in project vcell by virtualcell.

the class RunRefSimulationFastOp method saveExternalData.

private void saveExternalData(Image image, String varName, ExternalDataIdentifier newROIExtDataID, LocalWorkspace localWorkspace) throws ObjectNotFoundException, ImageException, IOException {
    Extent extent = image.getExtent();
    Origin origin = image.getOrigin();
    ISize isize = image.getISize();
    VCImage vcImage = new VCImageUncompressed(null, new byte[isize.getXYZ()], extent, isize.getX(), isize.getY(), isize.getZ());
    RegionImage regionImage = new RegionImage(vcImage, 0, null, null, RegionImage.NO_SMOOTHING);
    CartesianMesh simpleCartesianMesh = CartesianMesh.createSimpleCartesianMesh(origin, extent, isize, regionImage);
    int NumTimePoints = 1;
    int NumChannels = 1;
    // dimensions: time points, channels, whole image ordered by z slices.
    double[][][] pixData = new double[NumTimePoints][NumChannels][];
    pixData[0][0] = image.getDoublePixels();
    FieldDataFileOperationSpec fdos = new FieldDataFileOperationSpec();
    fdos.opType = FieldDataFileOperationSpec.FDOS_ADD;
    fdos.cartesianMesh = simpleCartesianMesh;
    fdos.doubleSpecData = pixData;
    fdos.specEDI = newROIExtDataID;
    fdos.varNames = new String[] { varName };
    fdos.owner = LocalWorkspace.getDefaultOwner();
    fdos.times = new double[] { 0.0 };
    fdos.variableTypes = new VariableType[] { VariableType.VOLUME };
    fdos.origin = origin;
    fdos.extent = extent;
    fdos.isize = isize;
    localWorkspace.getDataSetControllerImpl().fieldDataFileOperation(fdos);
}
Also used : Origin(org.vcell.util.Origin) CartesianMesh(cbit.vcell.solvers.CartesianMesh) Extent(org.vcell.util.Extent) ISize(org.vcell.util.ISize) FieldDataFileOperationSpec(cbit.vcell.field.io.FieldDataFileOperationSpec) RegionImage(cbit.vcell.geometry.RegionImage) VCImage(cbit.image.VCImage) VCImageUncompressed(cbit.image.VCImageUncompressed)

Example 57 with Origin

use of org.vcell.util.Origin in project vcell by virtualcell.

the class FluorescenceNoiseTest method testImageTimeSeries.

private void testImageTimeSeries(boolean bNoise, boolean bConvolve) throws ImageException, IOException {
    Origin origin = new Origin(0, 0, 0);
    Extent extent = new Extent(10, 10, 1);
    int numX = 30;
    int numY = 30;
    // typically 10000 or 100000;
    int numParticles = 40000;
    double diffusionRate = 1;
    double bleachRadius = 5 * 5;
    double psfVar = 0.6 * 0.6;
    ImageTimeSeries<UShortImage> rawTimeSeries = generateTestData(origin, extent, numX, numY, numParticles, diffusionRate, psfVar, bleachRadius, bNoise, bConvolve);
    new DisplayTimeSeriesOp().displayImageTimeSeries(rawTimeSeries, "time series", null);
}
Also used : Origin(org.vcell.util.Origin) DisplayTimeSeriesOp(org.vcell.vmicro.op.display.DisplayTimeSeriesOp) Extent(org.vcell.util.Extent) UShortImage(cbit.vcell.VirtualMicroscopy.UShortImage)

Example 58 with Origin

use of org.vcell.util.Origin in project vcell by virtualcell.

the class FluorescenceNoiseTest method doit.

private void doit() throws ImageException {
    int[] sizes = new int[] { 1, 2, 4, 8, 16, 32, 64, 128, 256 };
    for (int imageSize : sizes) {
        ISize size = new ISize(imageSize, imageSize, 1);
        Extent extent = new Extent(1, 1, 1);
        Origin origin = new Origin(0, 0, 0);
        // NormalizedSampleFunction sampleFunction = NormalizedSampleFunction.createUniform("uniformROI", origin, extent, size);
        NormalizedSampleFunction sampleFunction = NormalizedSampleFunction.fromGaussian("testGaussian", origin, extent, size, 0.5, 0.2, 0.1);
        SampleStatistics[] samples = new SampleStatistics[NUM_TRIALS];
        for (int i = 0; i < NUM_TRIALS; i++) {
            UShortImage rawImage = getUniformFluorescenceImage(size, extent, origin, MEAN_INTENSITY);
            samples[i] = sampleFunction.sample(rawImage);
        }
        Mean mean = new Mean();
        Variance var = new Variance();
        double[] weightedMeans = getWeightedMeans(samples);
        double[] weightedVariances = getWeightedVariances(samples);
        double weightedMeansVariance = var.evaluate(weightedMeans);
        double weightedMeansMean = mean.evaluate(weightedMeans);
        double weightedVarVariance = var.evaluate(weightedVariances);
        double weightedVarMean = mean.evaluate(weightedVariances);
        double V1 = samples[0].sumOfWeights;
        double V2 = samples[0].sumOfWeightsSquared;
        System.out.println("image is " + imageSize + "x" + imageSize + ", V1=" + V1 + ", V2=" + V2 + ", numTrials=" + NUM_TRIALS + ", sample means (mu=" + weightedMeansMean + ",s=" + weightedMeansVariance + "), sample variances (mu=" + weightedVarMean + ",s=" + weightedVarVariance);
    }
}
Also used : Origin(org.vcell.util.Origin) Mean(org.apache.commons.math3.stat.descriptive.moment.Mean) Extent(org.vcell.util.Extent) ISize(org.vcell.util.ISize) SampleStatistics(org.vcell.vmicro.workflow.data.NormalizedSampleFunction.SampleStatistics) UShortImage(cbit.vcell.VirtualMicroscopy.UShortImage) Variance(org.apache.commons.math3.stat.descriptive.moment.Variance) NormalizedSampleFunction(org.vcell.vmicro.workflow.data.NormalizedSampleFunction)

Example 59 with Origin

use of org.vcell.util.Origin in project vcell by virtualcell.

the class DisplayTimeSeries method displayImageTimeSeries.

public static void displayImageTimeSeries(final ImageTimeSeries<Image> imageTimeSeries, String title, WindowListener windowListener) throws ImageException, IOException {
    ISize size = imageTimeSeries.getISize();
    int dimension = (size.getZ() > 0) ? (3) : (2);
    Extent extent = imageTimeSeries.getExtent();
    Origin origin = imageTimeSeries.getAllImages()[0].getOrigin();
    // don't care ... no surfaces
    double filterCutoffFrequency = 0.5;
    VCImage vcImage = new VCImageUncompressed(null, new byte[size.getXYZ()], extent, size.getX(), size.getY(), size.getZ());
    RegionImage regionImage = new RegionImage(vcImage, dimension, extent, origin, filterCutoffFrequency);
    final CartesianMesh mesh = CartesianMesh.createSimpleCartesianMesh(origin, extent, size, regionImage);
    final DataIdentifier dataIdentifier = new DataIdentifier("var", VariableType.VOLUME, new Domain("domain"), false, "var");
    final DataSetController dataSetController = new DataSetController() {

        @Override
        public ExportEvent makeRemoteFile(OutputContext outputContext, ExportSpecs exportSpecs) throws DataAccessException, RemoteProxyException {
            throw new RuntimeException("not yet implemented");
        }

        @Override
        public TimeSeriesJobResults getTimeSeriesValues(OutputContext outputContext, VCDataIdentifier vcdataID, TimeSeriesJobSpec timeSeriesJobSpec) throws RemoteProxyException, DataAccessException {
            throw new RuntimeException("not yet implemented");
        }

        @Override
        public SimDataBlock getSimDataBlock(OutputContext outputContext, VCDataIdentifier vcdataID, String varName, double time) throws RemoteProxyException, DataAccessException {
            double timePoint = time;
            double[] timePoints = getDataSetTimes(vcdataID);
            int index = -1;
            for (int i = 0; i < timePoints.length; i++) {
                if (timePoint == timePoints[i]) {
                    index = i;
                    break;
                }
            }
            double[] data = imageTimeSeries.getAllImages()[index].getDoublePixels();
            PDEDataInfo pdeDataInfo = new PDEDataInfo(null, null, varName, time, 0);
            VariableType varType = VariableType.VOLUME;
            return new SimDataBlock(pdeDataInfo, data, varType);
        }

        @Override
        public boolean getParticleDataExists(VCDataIdentifier vcdataID) throws DataAccessException, RemoteProxyException {
            return false;
        }

        @Override
        public ParticleDataBlock getParticleDataBlock(VCDataIdentifier vcdataID, double time) throws DataAccessException, RemoteProxyException {
            return null;
        }

        @Override
        public ODESimData getODEData(VCDataIdentifier vcdataID) throws DataAccessException, RemoteProxyException {
            return null;
        }

        @Override
        public CartesianMesh getMesh(VCDataIdentifier vcdataID) throws RemoteProxyException, DataAccessException {
            return mesh;
        }

        @Override
        public PlotData getLineScan(OutputContext outputContext, VCDataIdentifier vcdataID, String variable, double time, SpatialSelection spatialSelection) throws RemoteProxyException, DataAccessException {
            throw new RuntimeException("not yet implemented");
        }

        @Override
        public AnnotatedFunction[] getFunctions(OutputContext outputContext, VCDataIdentifier vcdataID) throws DataAccessException, RemoteProxyException {
            return new AnnotatedFunction[0];
        }

        @Override
        public double[] getDataSetTimes(VCDataIdentifier vcdataID) throws RemoteProxyException, DataAccessException {
            return imageTimeSeries.getImageTimeStamps();
        }

        @Override
        public DataSetTimeSeries getDataSetTimeSeries(VCDataIdentifier vcdataID, String[] variableNames) throws DataAccessException, RemoteProxyException {
            throw new RuntimeException("not yet implemented");
        }

        @Override
        public DataSetMetadata getDataSetMetadata(VCDataIdentifier vcdataID) throws DataAccessException, RemoteProxyException {
            throw new RuntimeException("not yet implemented");
        }

        @Override
        public DataIdentifier[] getDataIdentifiers(OutputContext outputContext, VCDataIdentifier vcdataID) throws RemoteProxyException, DataAccessException {
            return new DataIdentifier[] { dataIdentifier };
        }

        @Override
        public FieldDataFileOperationResults fieldDataFileOperation(FieldDataFileOperationSpec fieldDataFileOperationSpec) throws RemoteProxyException, DataAccessException {
            throw new RuntimeException("not yet implemented");
        }

        @Override
        public DataOperationResults doDataOperation(DataOperation dataOperation) throws DataAccessException, RemoteProxyException {
            throw new RuntimeException("not yet implemented");
        }

        @Override
        public VtuFileContainer getEmptyVtuMeshFiles(VCDataIdentifier vcdataID, int timeIndex) throws RemoteProxyException, DataAccessException {
            throw new RuntimeException("not yet implemented");
        }

        @Override
        public double[] getVtuTimes(VCDataIdentifier vcdataID) throws RemoteProxyException, DataAccessException {
            throw new RuntimeException("not yet implemented");
        }

        @Override
        public double[] getVtuMeshData(OutputContext outputContext, VCDataIdentifier vcdataID, VtuVarInfo var, double time) throws RemoteProxyException, DataAccessException {
            // TODO Auto-generated method stub
            return null;
        }

        @Override
        public VtuVarInfo[] getVtuVarInfos(OutputContext outputContext, VCDataIdentifier vcDataIdentifier) throws DataAccessException, RemoteProxyException {
            // TODO Auto-generated method stub
            return null;
        }

        @Override
        public NFSimMolecularConfigurations getNFSimMolecularConfigurations(VCDataIdentifier vcdataID) throws RemoteProxyException, DataAccessException {
            // TODO Auto-generated method stub
            return null;
        }
    };
    DataSetControllerProvider dataSetControllerProvider = new DataSetControllerProvider() {

        @Override
        public DataSetController getDataSetController() throws DataAccessException {
            return dataSetController;
        }
    };
    VCDataManager vcDataManager = new VCDataManager(dataSetControllerProvider);
    OutputContext outputContext = new OutputContext(new AnnotatedFunction[0]);
    VCDataIdentifier vcDataIdentifier = new VCDataIdentifier() {

        public User getOwner() {
            return new User("nouser", null);
        }

        public KeyValue getDataKey() {
            return null;
        }

        public String getID() {
            return "mydata";
        }
    };
    PDEDataManager pdeDataManager = new PDEDataManager(outputContext, vcDataManager, vcDataIdentifier);
    ClientPDEDataContext myPdeDataContext = new ClientPDEDataContext(pdeDataManager);
    PDEDataViewer pdeDataViewer = new PDEDataViewer();
    JFrame jframe = new JFrame();
    jframe.setTitle(title);
    jframe.getContentPane().add(pdeDataViewer);
    jframe.setSize(1000, 600);
    jframe.setVisible(true);
    if (windowListener != null) {
        jframe.addWindowListener(windowListener);
    }
    pdeDataViewer.setPdeDataContext(myPdeDataContext);
}
Also used : Origin(org.vcell.util.Origin) VtuVarInfo(org.vcell.vis.io.VtuVarInfo) VCDataIdentifier(org.vcell.util.document.VCDataIdentifier) DataIdentifier(cbit.vcell.simdata.DataIdentifier) User(org.vcell.util.document.User) TimeSeriesJobSpec(org.vcell.util.document.TimeSeriesJobSpec) Extent(org.vcell.util.Extent) ISize(org.vcell.util.ISize) ExportSpecs(cbit.vcell.export.server.ExportSpecs) FieldDataFileOperationSpec(cbit.vcell.field.io.FieldDataFileOperationSpec) VCImage(cbit.image.VCImage) PDEDataInfo(cbit.vcell.simdata.PDEDataInfo) DataSetControllerProvider(cbit.vcell.server.DataSetControllerProvider) SimDataBlock(cbit.vcell.simdata.SimDataBlock) SpatialSelection(cbit.vcell.simdata.SpatialSelection) JFrame(javax.swing.JFrame) VCDataManager(cbit.vcell.simdata.VCDataManager) AnnotatedFunction(cbit.vcell.solver.AnnotatedFunction) DataOperation(cbit.vcell.simdata.DataOperation) VariableType(cbit.vcell.math.VariableType) DataSetController(cbit.vcell.server.DataSetController) VCImageUncompressed(cbit.image.VCImageUncompressed) OutputContext(cbit.vcell.simdata.OutputContext) CartesianMesh(cbit.vcell.solvers.CartesianMesh) PDEDataManager(cbit.vcell.simdata.PDEDataManager) RegionImage(cbit.vcell.geometry.RegionImage) ClientPDEDataContext(cbit.vcell.simdata.ClientPDEDataContext) Domain(cbit.vcell.math.Variable.Domain) VCDataIdentifier(org.vcell.util.document.VCDataIdentifier) PDEDataViewer(cbit.vcell.client.data.PDEDataViewer)

Example 60 with Origin

use of org.vcell.util.Origin in project vcell by virtualcell.

the class FitTimeSeries method main.

public static void main(String[] args) {
    try {
        int numX = 100;
        int numY = 100;
        double center_i = 52.44;
        double center_j = 51.39;
        double K = 30;
        double high = 0.9;
        double sigma2 = 4;
        float[] pixels = new float[numX * numY];
        Origin origin = new Origin(0, 0, 0);
        Extent extent = new Extent(1, 1, 1);
        FloatImage image = new FloatImage(pixels, origin, extent, numX, numY, 1);
        int index = 0;
        for (int j = 0; j < numY; j++) {
            for (int i = 0; i < numX; i++) {
                double radius = ((i - center_i) * (i - center_i) + (j - center_j) * (j - center_j));
                pixels[index++] = (float) (high - FastMath.exp(-K * FastMath.exp(-radius / sigma2)));
            }
        }
        double init_center_i = 50;
        double init_center_j = 50;
        double init_sigma2 = 2;
        GaussianFitResults fitResults = fitToGaussian(init_center_i, init_center_j, init_sigma2, image);
        System.out.println(fitResults);
    } catch (Exception e) {
        e.printStackTrace();
    }
}
Also used : Origin(org.vcell.util.Origin) FloatImage(cbit.vcell.VirtualMicroscopy.FloatImage) Extent(org.vcell.util.Extent)

Aggregations

Origin (org.vcell.util.Origin)64 Extent (org.vcell.util.Extent)58 ISize (org.vcell.util.ISize)45 CartesianMesh (cbit.vcell.solvers.CartesianMesh)18 VCImageUncompressed (cbit.image.VCImageUncompressed)17 FieldDataFileOperationSpec (cbit.vcell.field.io.FieldDataFileOperationSpec)17 ImageException (cbit.image.ImageException)14 RegionImage (cbit.vcell.geometry.RegionImage)14 VCImage (cbit.image.VCImage)13 ArrayList (java.util.ArrayList)13 Expression (cbit.vcell.parser.Expression)12 IOException (java.io.IOException)12 SubVolume (cbit.vcell.geometry.SubVolume)11 File (java.io.File)11 UShortImage (cbit.vcell.VirtualMicroscopy.UShortImage)10 Geometry (cbit.vcell.geometry.Geometry)9 ExternalDataIdentifier (org.vcell.util.document.ExternalDataIdentifier)8 ImageDataset (cbit.vcell.VirtualMicroscopy.ImageDataset)7 BioModel (cbit.vcell.biomodel.BioModel)7 AnalyticSubVolume (cbit.vcell.geometry.AnalyticSubVolume)7