use of uk.ac.sussex.gdsc.core.ij.gui.ExtendedGenericDialog in project GDSC-SMLM by aherbert.
the class ResequenceResults method showDialog.
private boolean showDialog() {
settings = Settings.load();
final ExtendedGenericDialog gd = new ExtendedGenericDialog(TITLE);
gd.addHelp(HelpUrls.getUrl("resequence-results"));
gd.addMessage("Resequence the results in memory (assumed to be continuous from 1).\n" + "Describe the regular repeat of the original image:\n" + "Start = The first frame that contained the data\n" + "Block = The number of continuous frames containing data\n" + "Skip = The number of continuous frames to ignore before the next data\n \n" + "E.G. 2:9:1 = Data was imaged from frame 2 for 9 frames, 1 frame to ignore," + " then repeat.");
ResultsManager.addInput(gd, settings.inputOption, InputSource.MEMORY);
gd.addNumericField("Start", settings.start, 0);
gd.addNumericField("Block", settings.block, 0);
gd.addNumericField("Skip", settings.skip, 0);
gd.addCheckbox("Log_mapping", settings.logMapping);
gd.showDialog();
if (gd.wasCanceled()) {
return false;
}
settings.inputOption = ResultsManager.getInputSource(gd);
settings.start = (int) gd.getNextNumber();
settings.block = (int) gd.getNextNumber();
settings.skip = (int) gd.getNextNumber();
settings.logMapping = gd.getNextBoolean();
settings.save();
// Check arguments
try {
ParameterUtils.isAboveZero("Start", settings.start);
ParameterUtils.isAboveZero("Block", settings.block);
ParameterUtils.isPositive("Skip", settings.skip);
} catch (final IllegalArgumentException ex) {
IJ.error(TITLE, ex.getMessage());
return false;
}
return true;
}
use of uk.ac.sussex.gdsc.core.ij.gui.ExtendedGenericDialog in project GDSC-SMLM by aherbert.
the class PsfDrift method run.
@Override
public void run(String arg) {
SmlmUsageTracker.recordPlugin(this.getClass(), arg);
if ("hwhm".equals(arg)) {
showHwhm();
return;
}
// Build a list of suitable images
final List<String> titles = createImageList(true);
if (titles.isEmpty()) {
IJ.error(TITLE, "No suitable PSF images");
return;
}
final ExtendedGenericDialog gd = new ExtendedGenericDialog(TITLE);
settings = Settings.load();
fitConfig = fitConfigRef.get().createCopy();
gd.addMessage("Select the input PSF image");
gd.addChoice("PSF", titles.toArray(new String[0]), settings.title);
gd.addCheckbox("Use_offset", settings.useOffset);
gd.addNumericField("Scale", settings.scale, 2);
gd.addNumericField("z_depth", settings.zDepth, 2, 6, "nm");
gd.addNumericField("Grid_size", settings.gridSize, 0);
gd.addSlider("Recall_limit", 0.01, 1, settings.recallLimit);
gd.addSlider("Region_size", 2, 20, settings.regionSize);
gd.addCheckbox("Background_fitting", settings.backgroundFitting);
PeakFit.addPsfOptions(gd, fitConfig);
gd.addChoice("Fit_solver", SettingsManager.getFitSolverNames(), FitProtosHelper.getName(fitConfig.getFitSolver()));
// We need these to set bounds for any bounded fitters
gd.addSlider("Min_width_factor", 0, 0.99, fitConfig.getMinWidthFactor());
gd.addSlider("Width_factor", 1, 4.5, fitConfig.getMaxWidthFactor());
gd.addCheckbox("Offset_fit", settings.offsetFitting);
gd.addNumericField("Start_offset", settings.startOffset, 3);
gd.addCheckbox("Include_CoM_fit", settings.comFitting);
gd.addCheckbox("Use_sampling", settings.useSampling);
gd.addNumericField("Photons", settings.photons, 0);
gd.addSlider("Photon_limit", 0, 1, settings.photonLimit);
gd.addSlider("Smoothing", 0, 0.5, settings.smoothing);
gd.addHelp(HelpUrls.getUrl("psf-drift"));
gd.showDialog();
if (gd.wasCanceled()) {
return;
}
settings.title = gd.getNextChoice();
settings.useOffset = gd.getNextBoolean();
settings.scale = gd.getNextNumber();
settings.zDepth = gd.getNextNumber();
settings.gridSize = (int) gd.getNextNumber();
settings.recallLimit = gd.getNextNumber();
settings.regionSize = (int) Math.abs(gd.getNextNumber());
settings.backgroundFitting = gd.getNextBoolean();
fitConfig.setPsfType(PeakFit.getPsfTypeValues()[gd.getNextChoiceIndex()]);
// Some enum values are not supported
fitConfig.setFitSolver(SettingsManager.getFitSolverValues()[gd.getNextChoiceIndex()]);
fitConfig.setMinWidthFactor(gd.getNextNumber());
fitConfig.setMaxWidthFactor(gd.getNextNumber());
settings.offsetFitting = gd.getNextBoolean();
settings.startOffset = Math.abs(gd.getNextNumber());
settings.comFitting = gd.getNextBoolean();
settings.useSampling = gd.getNextBoolean();
settings.photons = Math.abs(gd.getNextNumber());
settings.photonLimit = Math.abs(gd.getNextNumber());
settings.smoothing = Math.abs(gd.getNextNumber());
settings.save();
fitConfigRef.set(fitConfig);
gd.collectOptions();
if (!settings.comFitting && !settings.offsetFitting) {
IJ.error(TITLE, "No initial fitting positions");
return;
}
if (settings.regionSize < 1) {
settings.regionSize = 1;
}
if (gd.invalidNumber()) {
return;
}
imp = WindowManager.getImage(settings.title);
if (imp == null) {
IJ.error(TITLE, "No PSF image for image: " + settings.title);
return;
}
psfSettings = getPsfSettings(imp);
if (psfSettings == null) {
IJ.error(TITLE, "No PSF settings for image: " + settings.title);
return;
}
// Configure the fit solver. We must wrap the settings with a
// FitEngineConfiguration to pass to the PeakFit method
final FitEngineSettings fitEngineSettings = FitProtosHelper.defaultFitEngineSettings;
final FitEngineConfiguration config = new FitEngineConfiguration(fitEngineSettings, SettingsManager.readCalibration(0), PsfProtosHelper.defaultOneAxisGaussian2DPSF);
config.getFitConfiguration().setFitSettings(fitConfig.getFitSettings());
if (!PeakFit.configurePsfModel(config)) {
return;
}
if (!PeakFit.configureFitSolver(config, IJImageSource.getBounds(imp), null, PeakFit.FLAG_NO_SAVE)) {
return;
}
// Update configuration
fitConfig = config.getFitConfiguration();
fitConfigRef.set(fitConfig);
computeDrift();
}
use of uk.ac.sussex.gdsc.core.ij.gui.ExtendedGenericDialog in project GDSC-SMLM by aherbert.
the class PeakFit method getExposureTime.
private static boolean getExposureTime(CalibrationWriter calibration) {
final ExtendedGenericDialog gd = newWizardDialog("Enter the exposure time. Calibration of the exposure time allows correct reporting of on" + " and off times.", "This is the length of time for each frame in the image.");
gd.addNumericField("Exposure_time", calibration.getExposureTime(), 2, 6, "ms");
gd.showDialog();
if (gd.wasCanceled()) {
return false;
}
calibration.setExposureTime(Math.abs(gd.getNextNumber()));
return true;
}
use of uk.ac.sussex.gdsc.core.ij.gui.ExtendedGenericDialog in project GDSC-SMLM by aherbert.
the class PeakFit method getCameraType.
private static boolean getCameraType(CalibrationWriter calibration) {
final ExtendedGenericDialog gd = newWizardDialog("Enter the type of camera.");
gd.addChoice("Camera_type", SettingsManager.getCameraTypeNames(), CalibrationProtosHelper.getName(calibration.getCameraType()));
gd.showDialog();
if (gd.wasCanceled()) {
return false;
}
calibration.setCameraType(SettingsManager.getCameraTypeValues()[gd.getNextChoiceIndex()]);
if (!calibration.isCcdCamera()) {
// TODO - Support sCMOS camera
IJ.error("Unsupported camera type " + CalibrationProtosHelper.getName(calibration.getCameraType()));
return false;
}
return true;
}
use of uk.ac.sussex.gdsc.core.ij.gui.ExtendedGenericDialog in project GDSC-SMLM by aherbert.
the class PeakFit method configureFitSolver.
/**
* Show a dialog to configure the fit solver. The updated settings are saved to the settings file.
* An error message is shown if the dialog is cancelled or the configuration is invalid.
*
* <p>The bounds are used to validate the camera model. The camera model must be large enough to
* cover the source bounds. If larger then it will be cropped. Optionally an internal region of
* the input image can be specified. This is relative to the width and height of the input image.
* If no camera model is present then the bounds can be null.
*
* @param config the configuration
* @param sourceBounds the source image bounds (used to validate the camera model dimensions)
* @param bounds the crop bounds (relative to the input image, used to validate the camera model
* dimensions)
* @param flags the flags
* @return True if the configuration succeeded
*/
public static boolean configureFitSolver(FitEngineConfiguration config, Rectangle sourceBounds, Rectangle bounds, int flags) {
final boolean extraOptions = BitFlagUtils.anySet(flags, FLAG_EXTRA_OPTIONS);
final boolean ignoreCalibration = BitFlagUtils.anySet(flags, FLAG_IGNORE_CALIBRATION);
final boolean saveSettings = BitFlagUtils.anyNotSet(flags, FLAG_NO_SAVE);
final FitConfiguration fitConfig = config.getFitConfiguration();
final CalibrationWriter calibration = fitConfig.getCalibrationWriter();
final FitSolver fitSolver = fitConfig.getFitSolver();
final boolean isLvm = fitSolver == FitSolver.LVM_LSE || fitSolver == FitSolver.LVM_WLSE || fitSolver == FitSolver.LVM_MLE;
// Support the deprecated backtracking FastMLE solver as a plain FastMLE solver
final boolean isFastMml = fitSolver == FitSolver.FAST_MLE || fitSolver == FitSolver.BACKTRACKING_FAST_MLE;
final boolean isSteppingFunctionSolver = isLvm || isFastMml;
if (fitSolver == FitSolver.MLE) {
final ExtendedGenericDialog gd = new ExtendedGenericDialog(TITLE);
if (!ignoreCalibration) {
gd.addMessage("Maximum Likelihood Estimation requires CCD-type camera parameters");
gd.addNumericField("Camera_bias", calibration.getBias(), 2, 6, "count");
gd.addCheckbox("Model_camera_noise", fitConfig.isModelCamera());
gd.addNumericField("Read_noise", calibration.getReadNoise(), 2, 6, "count");
gd.addNumericField("Quantum_efficiency", calibration.getQuantumEfficiency(), 2, 6, "electron/photon");
gd.addCheckbox("EM-CCD", calibration.isEmCcd());
} else {
gd.addMessage("Maximum Likelihood Estimation requires additional parameters");
}
final String[] searchNames = SettingsManager.getSearchMethodNames();
gd.addChoice("Search_method", searchNames, FitProtosHelper.getName(fitConfig.getSearchMethod()));
gd.addStringField("Relative_threshold", MathUtils.rounded(fitConfig.getRelativeThreshold()));
gd.addStringField("Absolute_threshold", MathUtils.rounded(fitConfig.getAbsoluteThreshold()));
gd.addNumericField("Max_iterations", fitConfig.getMaxIterations(), 0);
gd.addNumericField("Max_function_evaluations", fitConfig.getMaxFunctionEvaluations(), 0);
if (extraOptions) {
gd.addCheckbox("Gradient_line_minimisation", fitConfig.isGradientLineMinimisation());
}
gd.showDialog();
if (gd.wasCanceled()) {
return false;
}
if (!ignoreCalibration) {
calibration.setBias(Math.abs(gd.getNextNumber()));
fitConfig.setModelCamera(gd.getNextBoolean());
calibration.setReadNoise(Math.abs(gd.getNextNumber()));
calibration.setQuantumEfficiency(Math.abs(gd.getNextNumber()));
calibration.setCameraType((gd.getNextBoolean()) ? CameraType.EMCCD : CameraType.CCD);
fitConfig.setCalibration(calibration.getCalibration());
}
fitConfig.setSearchMethod(SettingsManager.getSearchMethodValues()[gd.getNextChoiceIndex()]);
fitConfig.setRelativeThreshold(getThresholdNumber(gd));
fitConfig.setAbsoluteThreshold(getThresholdNumber(gd));
fitConfig.setMaxIterations((int) gd.getNextNumber());
fitConfig.setMaxFunctionEvaluations((int) gd.getNextNumber());
if (extraOptions) {
fitConfig.setGradientLineMinimisation(gd.getNextBoolean());
} else {
// This option is for the Conjugate Gradient optimiser and makes it less stable
fitConfig.setGradientLineMinimisation(false);
}
if (saveSettings) {
saveFitEngineSettings(config);
}
try {
ParameterUtils.isAboveZero("Relative threshold", fitConfig.getRelativeThreshold());
ParameterUtils.isAboveZero("Absolute threshold", fitConfig.getAbsoluteThreshold());
ParameterUtils.isAboveZero("Max iterations", fitConfig.getMaxIterations());
ParameterUtils.isAboveZero("Max function evaluations", fitConfig.getMaxFunctionEvaluations());
fitConfig.getFunctionSolver();
} catch (final IllegalArgumentException | IllegalStateException ex) {
IJ.error(TITLE, ex.getMessage());
return false;
}
} else if (isSteppingFunctionSolver) {
final boolean requireCalibration = !ignoreCalibration && fitSolver != FitSolver.LVM_LSE;
// Collect options for LVM fitting
final ExtendedGenericDialog gd = new ExtendedGenericDialog(TITLE);
final String fitSolverName = FitProtosHelper.getName(fitSolver);
gd.addMessage(fitSolverName + " requires additional parameters");
gd.addStringField("Relative_threshold", MathUtils.rounded(fitConfig.getRelativeThreshold()));
gd.addStringField("Absolute_threshold", MathUtils.rounded(fitConfig.getAbsoluteThreshold()));
gd.addStringField("Parameter_relative_threshold", MathUtils.rounded(fitConfig.getParameterRelativeThreshold()));
gd.addStringField("Parameter_absolute_threshold", MathUtils.rounded(fitConfig.getParameterAbsoluteThreshold()));
gd.addNumericField("Max_iterations", fitConfig.getMaxIterations(), 0);
if (isLvm) {
gd.addNumericField("Lambda", fitConfig.getLambda(), 4);
}
if (isFastMml) {
gd.addCheckbox("Fixed_iterations", fitConfig.isFixedIterations());
// This works because the proto configuration enum matches the named enum
final String[] lineSearchNames = SettingsManager.getNames((Object[]) FastMleSteppingFunctionSolver.LineSearchMethod.values());
gd.addChoice("Line_search_method", lineSearchNames, lineSearchNames[fitConfig.getLineSearchMethod().getNumber()]);
}
gd.addCheckbox("Use_clamping", fitConfig.isUseClamping());
gd.addCheckbox("Dynamic_clamping", fitConfig.isUseDynamicClamping());
final PSF psf = fitConfig.getPsf();
final boolean isAstigmatism = psf.getPsfType() == PSFType.ASTIGMATIC_GAUSSIAN_2D;
final int nParams = PsfHelper.getParameterCount(psf);
if (extraOptions) {
gd.addNumericField("Clamp_background", fitConfig.getClampBackground(), 2);
gd.addNumericField("Clamp_signal", fitConfig.getClampSignal(), 2);
gd.addNumericField("Clamp_x", fitConfig.getClampX(), 2);
gd.addNumericField("Clamp_y", fitConfig.getClampY(), 2);
if (isAstigmatism) {
gd.addNumericField("Clamp_z", fitConfig.getClampZ(), 2);
} else {
if (nParams > 1 || !fitConfig.isFixedPsf()) {
gd.addNumericField("Clamp_sx", fitConfig.getClampXSd(), 2);
}
if (nParams > 1) {
gd.addNumericField("Clamp_sy", fitConfig.getClampYSd(), 2);
}
if (nParams > 2) {
gd.addNumericField("Clamp_angle", fitConfig.getClampAngle(), 2);
}
}
}
// Extra parameters are needed for calibrated fit solvers
if (requireCalibration) {
switch(calibration.getCameraType()) {
case CCD:
case EMCCD:
case SCMOS:
break;
default:
IJ.error(TITLE, fitSolverName + " requires camera calibration");
return false;
}
gd.addMessage(fitSolverName + " requires calibration for camera: " + CalibrationProtosHelper.getName(calibration.getCameraType()));
if (calibration.isScmos()) {
final String[] models = CameraModelManager.listCameraModels(true);
gd.addChoice("Camera_model_name", models, fitConfig.getCameraModelName());
} else {
gd.addNumericField("Camera_bias", calibration.getBias(), 2, 6, "Count");
gd.addNumericField("Gain", calibration.getCountPerPhoton(), 2, 6, "Count/photon");
gd.addNumericField("Read_noise", calibration.getReadNoise(), 2, 6, "Count");
}
}
gd.showDialog();
if (gd.wasCanceled()) {
return false;
}
fitConfig.setRelativeThreshold(getThresholdNumber(gd));
fitConfig.setAbsoluteThreshold(getThresholdNumber(gd));
fitConfig.setParameterRelativeThreshold(getThresholdNumber(gd));
fitConfig.setParameterAbsoluteThreshold(getThresholdNumber(gd));
fitConfig.setMaxIterations((int) gd.getNextNumber());
if (isLvm) {
fitConfig.setLambda(gd.getNextNumber());
}
if (isFastMml) {
fitConfig.setFixedIterations(gd.getNextBoolean());
fitConfig.setLineSearchMethod(gd.getNextChoiceIndex());
}
fitConfig.setUseClamping(gd.getNextBoolean());
fitConfig.setUseDynamicClamping(gd.getNextBoolean());
if (extraOptions) {
fitConfig.setClampBackground(Math.abs(gd.getNextNumber()));
fitConfig.setClampSignal(Math.abs(gd.getNextNumber()));
fitConfig.setClampX(Math.abs(gd.getNextNumber()));
fitConfig.setClampY(Math.abs(gd.getNextNumber()));
if (isAstigmatism) {
fitConfig.setClampZ(Math.abs(gd.getNextNumber()));
} else {
if (nParams > 1 || !fitConfig.isFixedPsf()) {
fitConfig.setClampXSd(Math.abs(gd.getNextNumber()));
}
if (nParams > 1) {
fitConfig.setClampYSd(Math.abs(gd.getNextNumber()));
}
if (nParams > 2) {
fitConfig.setClampAngle(Math.abs(gd.getNextNumber()));
}
}
}
if (requireCalibration) {
if (calibration.isScmos()) {
fitConfig.setCameraModelName(gd.getNextChoice());
} else {
calibration.setBias(Math.abs(gd.getNextNumber()));
calibration.setCountPerPhoton(Math.abs(gd.getNextNumber()));
calibration.setReadNoise(Math.abs(gd.getNextNumber()));
fitConfig.setCalibration(calibration.getCalibration());
}
}
// camera model is set.
if (calibration.isScmos()) {
fitConfig.setCameraModel(CameraModelManager.load(fitConfig.getCameraModelName()));
if (!checkCameraModel(fitConfig, sourceBounds, bounds, true)) {
return false;
}
}
if (saveSettings) {
saveFitEngineSettings(config);
}
try {
if (isLvm) {
ParameterUtils.isAboveZero("Lambda", fitConfig.getLambda());
}
// This call will check if the configuration is OK (including convergence criteria)
fitConfig.getFunctionSolver();
} catch (final IllegalArgumentException | IllegalStateException ex) {
IJ.error(TITLE, ex.getMessage());
return false;
}
} else {
IJ.error(TITLE, "Unknown fit solver: " + fitSolver);
return false;
}
if (config.isIncludeNeighbours() && !fitConfig.getFunctionSolver().isBounded()) {
IJ.error(TITLE, "Including neighbours requires a bounded fit solver");
return false;
}
return true;
}
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