Example 1 with BFSShortestPath
use of cz1.ngs.model.BFSShortestPath in project polyGembler by c-zhou.
the class Anchor method run.
// private static DijkstraShortestPath<Integer, DefaultWeightedEdge> dijkstra_paths;
@Override
public void run() {
// TODO Auto-generated method stub
long tic = System.nanoTime();
sub_seqs = Sequence.parseFastaFileAsMap(subject_file);
qry_seqs = Sequence.parseFastaFileWithRevCmpAsMap(query_file);
// this.makeAssemblyGraph(query_file);
this.makeAssemblyGraph(query_file);
bfs = new BFSShortestPath<Integer, DefaultWeightedEdge>(assembly_graph);
// dijkstra_paths = new DijkstraShortestPath<Integer, DefaultWeightedEdge>(assembly_graph);
// find 'N/n's in subject/reference sequences
// which could have impact on parsing the blast records
final Map<String, TreeRangeSet<Integer>> sub_gaps = new HashMap<String, TreeRangeSet<Integer>>();
final Map<String, List<Blast6Segment>> anchored_records = new HashMap<String, List<Blast6Segment>>();
for (Map.Entry<String, Sequence> entry : sub_seqs.entrySet()) {
String seq_sn = entry.getKey();
String seq_str = entry.getValue().seq_str();
final TreeRangeSet<Integer> tmp_rangeSet = TreeRangeSet.create();
for (int j = 0; j < seq_str.length(); j++) {
if (seq_str.charAt(j) == 'N' || seq_str.charAt(j) == 'n')
// blast record is 1-based closed coordination
tmp_rangeSet.add(Range.closed(j + 1, j + 1).canonical(DiscreteDomain.integers()));
}
int seq_ln = seq_str.length();
final TreeRangeSet<Integer> range_set = TreeRangeSet.create();
for (Range<Integer> range : tmp_rangeSet.asRanges()) {
int lowerend = range.hasLowerBound() ? Math.max(0, range.lowerEndpoint() - gap_buff) : 0;
int upperend = range.hasUpperBound() ? Math.min(seq_ln, range.upperEndpoint() + gap_buff - 1) : seq_ln;
range_set.add(Range.closed(lowerend, upperend).canonical(DiscreteDomain.integers()));
}
sub_gaps.put(seq_sn, range_set);
// initialise an array list for each reference chromosome
anchored_records.put(seq_sn, new ArrayList<Blast6Segment>());
}
// parse blast records
// blast records buffer
final List<Blast6Segment> buff = new ArrayList<Blast6Segment>();
// selected blast records
final List<Blast6Segment> sel_recs = new ArrayList<Blast6Segment>();
// temp list
final List<Blast6Segment> tmp_records = new ArrayList<Blast6Segment>();
try {
BufferedReader br_blast = Utils.getBufferedReader(blast_out);
Blast6Segment tmp_record = Blast6Segment.blast6Segment(br_blast.readLine());
Blast6Segment primary_record, secondary_record;
String qry;
double qry_ln, aln_frac;
while (tmp_record != null) {
qry = tmp_record.qseqid();
qry_ln = qry_seqs.get(qry).seq_ln();
buff.clear();
buff.add(tmp_record);
while ((tmp_record = Blast6Segment.blast6Segment(br_blast.readLine())) != null && tmp_record.qseqid().equals(qry)) {
// filter by identity
if (tmp_record.pident() >= this.min_ident)
buff.add(tmp_record);
}
sel_recs.clear();
// merge collinear records
for (String sub : sub_seqs.keySet()) {
// get all records for subject/reference sequence sub_seq
tmp_records.clear();
for (Blast6Segment record : buff) if (record.sseqid().equals(sub))
tmp_records.add(record);
if (tmp_records.isEmpty())
continue;
// find alignment segments that can be deleted
// those that are subsets of larger alignment segments
// (Sstart, (sstart, send), Send) and (Qstart, (qstart, qend), Qend)
Collections.sort(tmp_records, new Blast6Segment.SegmentSizeComparator());
final Set<int[][]> ranges = new HashSet<int[][]>();
outerloop: for (int i = 0; i < tmp_records.size(); i++) {
primary_record = tmp_records.get(i);
int[][] range = new int[2][2];
if (primary_record.sstart() < primary_record.send()) {
range[0][0] = primary_record.sstart();
range[0][1] = primary_record.send();
} else {
range[0][0] = primary_record.send();
range[0][1] = primary_record.sstart();
}
if (primary_record.qstart() < primary_record.qend()) {
range[1][0] = primary_record.qstart();
range[1][1] = primary_record.qend();
} else {
range[1][0] = primary_record.qend();
range[1][1] = primary_record.qstart();
}
for (int[][] r : ranges) {
if (r[0][0] <= range[0][0] && r[0][1] >= range[0][1] && r[1][0] <= range[1][0] && r[1][1] >= range[1][1]) {
tmp_records.remove(i--);
continue outerloop;
}
}
ranges.add(range);
}
// TODO rewrite this part
/**
* // find collinear alignment segments that can be merged
* Collections.sort(tmp_records, new BlastRecord.SInterceptComparator());
*
* collinear_merged.clear();
* final List<Blast6Record> temp = new ArrayList<Blast6Record>();
* for(int i=0; i<tmp_records.size(); ) {
* Blast6Record record = tmp_records.get(i);
* double max_shift;
* temp.clear();
* temp.add(record);
*
* // find collinear alignment segments
* outerloop:
* while( (++i)<tmp_records.size() ) {
* record = tmp_records.get(i);
* // check if is collinear with other alignment segments
* for(Blast6Record r : temp) {
* max_shift = collinear_shift*
* Math.min(r.length(), record.length());
* if(BlastRecord.sdistance(r, record)<=max_shift &&
* BlastRecord.qdistance(r, record)<=max_shift &&
* BlastRecord.pdistance(r, record)<=max_shift) {
* temp.add(record);
* continue outerloop;
* }
* }
* break;
* }
*
* // merge collinear alignment segments
* int qstart = Integer.MAX_VALUE;
* int qend = Integer.MIN_VALUE;
* int sstart = Integer.MAX_VALUE;
* int send = Integer.MIN_VALUE;
* double pident = 0;
* int length = 0;
*
* // qstart is always smaller than qend
* for(int j=0; j<temp.size(); j++) {
* record = temp.get(j);
* if(record.qstart()<qstart) {
* qstart = record.qstart();
* sstart = record.sstart();
* }
* if(record.qend()>qend) {
* qend = record.qend();
* send = record.send();
* }
* if(record.pident()>pident)
* pident = record.pident();
* length += record.length();
* }
*
* collinear_merged.add(new Blast6Record(qry,sub,pident,length,-1,-1,qstart,qend,sstart,send,-1,-1));
* }
*/
// find collinear alignment segments that can be merged
// more accurate but slower
Collections.sort(tmp_records, new AlignmentSegment.SubjectCoordinationComparator());
Blast6Segment record;
for (int i = 0; i < tmp_records.size(); i++) {
primary_record = tmp_records.get(i);
for (int j = i + 1; j < tmp_records.size(); j++) {
secondary_record = tmp_records.get(j);
double max_shift = collinear_shift * Math.min(primary_record.length(), secondary_record.length());
if ((record = Blast6Segment.collinear(primary_record, secondary_record, max_shift)) != null) {
tmp_records.set(i, record);
tmp_records.remove(j);
--i;
break;
}
}
}
// process blast records that clipped by gaps
// (sstart, send)---(start2, send2)
// (sstart ... --- ... send2)
TreeRangeSet<Integer> sub_gap = sub_gaps.get(sub);
Collections.sort(tmp_records, new AlignmentSegment.SubjectCoordinationComparator());
for (int i = 0; i < tmp_records.size(); i++) {
primary_record = tmp_records.get(i);
if (sub_gap.contains(primary_record.true_send())) {
secondary_record = null;
int sec_j = -1;
for (int j = i + 1; j < tmp_records.size(); j++) {
if (tmp_records.get(j).true_sstart() >= primary_record.true_send()) {
secondary_record = tmp_records.get(j);
sec_j = j;
break;
}
}
if (secondary_record == null || AlignmentSegment.reverse(primary_record, secondary_record)) {
// reverse alignment segments
continue;
}
if (sub_gap.contains(secondary_record.true_sstart()) && sub_gap.rangeContaining(primary_record.true_send()).equals(sub_gap.rangeContaining(secondary_record.true_sstart()))) {
// clipping
// merge two alignment segments
double pident = Math.max(primary_record.pident(), secondary_record.pident());
int qstart = Math.min(primary_record.true_qstart(), secondary_record.true_qstart());
int qend = Math.max(primary_record.true_qend(), secondary_record.true_qend());
int sstart = Math.min(primary_record.true_sstart(), secondary_record.true_sstart());
int send = Math.max(primary_record.true_send(), secondary_record.true_send());
int length = qend - qstart + 1;
// replace primary record with merged record
// delete secondary record
Blast6Segment merged_record = primary_record.forward() ? new Blast6Segment(qry, sub, pident, length, -1, -1, qstart, qend, sstart, send, -1, -1) : new Blast6Segment(qry, sub, pident, length, -1, -1, qstart, qend, send, sstart, -1, -1);
tmp_records.set(i, merged_record);
tmp_records.remove(sec_j);
// the merged records need to be processed
--i;
}
}
}
// add to sel_recs
sel_recs.addAll(tmp_records);
}
// filter by alignment fraction
buff.clear();
buff.addAll(sel_recs);
sel_recs.clear();
for (Blast6Segment record : buff) {
if (record.length() / qry_ln >= this.min_frac)
sel_recs.add(record);
}
if (sel_recs.isEmpty()) {
// continue
continue;
}
// filter blast records
Collections.sort(sel_recs, new Blast6Segment.MatchIndentityComparator());
// process primary alignment
primary_record = sel_recs.get(0);
anchored_records.get(primary_record.sseqid()).add(primary_record);
aln_frac = primary_record.length() / qry_ln;
// and process
for (int i = 1; i < sel_recs.size(); i++) {
secondary_record = sel_recs.get(i);
if (secondary_record.pident() + this.diff_ident < primary_record.pident() || secondary_record.length() / qry_ln + this.diff_frac < aln_frac) {
break;
} else {
anchored_records.get(secondary_record.sseqid()).add(secondary_record);
}
}
}
br_blast.close();
for (Map.Entry<String, List<Blast6Segment>> entry : anchored_records.entrySet()) {
System.out.println(entry.getKey() + ": " + entry.getValue().size());
}
final BufferedWriter bw_map = Utils.getBufferedWriter(out_prefix + ".map");
final BufferedWriter bw_fa = Utils.getBufferedWriter(out_prefix + ".fa");
final Set<String> anchored_seqs = new HashSet<String>();
final List<String> sub_list = Sequence.parseSeqList(subject_file);
for (String sub_sn : sub_list) {
blast6_records = anchored_records.get(sub_sn);
int nV = blast6_records.size(), count = 0;
// sort blast records
Collections.sort(blast6_records, new Blast6Segment.SubjectCoordinationComparator());
// consensus
int posUpto = 0, send_clip = 0;
int sstart, send, qstart, qend, qlen, tmp_int, qstart_clip, qend_clip, gap_size;
// distance to last 'N', start from next position to find longest common suffix-prefix
int prev_n = Integer.MAX_VALUE;
int mol_len = 0;
String qseq;
int nS, nQ;
// first step: construct super scaffold
// will form a tree graph indicating the path through the contigs
// convert contig names to integer indices
// one contig could end up with multiple indices due to repeats
Map<Integer, String> ss_coordinate = new HashMap<Integer, String>();
int index = 0;
for (Blast6Segment record : blast6_records) ss_coordinate.put(index++, record.qseqid() + (record.forward() ? "" : "'"));
StringBuilder seq_str = new StringBuilder();
for (int v = 0; v < nV - 1; v++) {
if (++count % 10000 == 0)
myLogger.info(sub_sn + " " + count + "/" + nV + " done.");
Blast6Segment record = blast6_records.get(v);
qlen = qry_seqs.get(record.qseqid()).seq_ln();
sstart = record.sstart();
send = record.send();
qstart = record.qstart();
qend = record.qend();
if (sstart > send) {
// make sure sstart<send
tmp_int = sstart;
sstart = send;
send = tmp_int;
tmp_int = qstart;
qstart = qend;
qend = tmp_int;
}
if (qstart > qend) {
qstart_clip = qlen - qstart;
qend_clip = qend - 1;
qseq = Sequence.revCompSeq(qry_seqs.get(record.qseqid()).seq_str());
} else {
qstart_clip = qstart - 1;
qend_clip = qlen - qend;
qseq = qry_seqs.get(record.qseqid()).seq_str();
}
if (send < posUpto || sstart < posUpto && qstart_clip > max_clip) {
// TODO process
continue;
}
// find longest suffix-prefix
nS = seq_str.length();
nQ = qseq.length();
int nO = Math.min(prev_n, Math.min(nS, nQ));
outerloop: for (; nO >= min_overlap; nO--) {
int nS_i = nS - nO;
for (int i = 0; i < nO; i++) {
if (seq_str.charAt(nS_i + i) != qseq.charAt(i))
continue outerloop;
}
break outerloop;
}
if (nO < min_overlap) {
// otherwise will insert a large GAP max(pseduo_distance, max_gap)
if (posUpto > 0) {
if (sstart <= posUpto) {
// if too much overlap, then treat it as a contradiction
if (posUpto - sstart > min_overlap) {
// discard
continue;
} else {
// insert a min_gap
gap_size = min_gap;
}
} else {
// estimate gap size
gap_size = (sstart - posUpto) - (send_clip + qstart_clip);
if (gap_size < max_gap)
gap_size = max_gap;
}
bw_map.write("GAP\t" + gap_size + "\t0\t" + gap_size + "\t+\t" + sub_sn + "\t" + mol_len + "\t" + (mol_len + gap_size) + "\n");
seq_str.append(Sequence.polyN(gap_size));
mol_len += gap_size;
}
bw_map.write(record.qseqid() + "\t" + qlen + "\t");
if (qstart > qend) {
// reverse
bw_map.write("0\t" + qlen + "\t-\t");
} else {
// forward
bw_map.write("0\t" + qlen + "\t+\t");
}
seq_str.append(qseq);
bw_map.write(sub_sn + "\t" + mol_len + "\t" + (mol_len + qlen) + "\n");
mol_len += qlen;
prev_n = qlen;
anchored_seqs.add(record.qseqid());
} else {
// overlap found
// will not insert gap
// ====================
// /-------\
// /----\
// calculate overlaps
// process overlap
qstart = nO;
if (qstart == qlen)
continue;
bw_map.write(record.qseqid() + "\t" + (qlen - qstart) + "\t");
if (qstart > qend) {
// reverse
bw_map.write(0 + "\t" + (qlen - qstart) + "\t-\t");
} else {
// forward
bw_map.write(qstart + "\t" + qlen + "\t+\t");
}
bw_map.write(sub_sn + "\t" + mol_len + "\t" + (mol_len + qlen - qstart) + "\n");
mol_len += qlen - qstart;
prev_n += qlen - qstart;
seq_str.append(qseq.substring(qstart));
anchored_seqs.add(record.qseqid());
}
posUpto = send;
send_clip = qend_clip;
}
if (seq_str.length() > 0)
bw_fa.write(Sequence.formatOutput(sub_sn, seq_str.toString()));
}
bw_fa.close();
bw_map.close();
final BufferedWriter bw_ufa = Utils.getBufferedWriter(out_prefix + "_unplaced.fa");
for (String seq : qry_seqs.keySet()) if (!anchored_seqs.contains(seq))
bw_ufa.write(qry_seqs.get(seq).formatOutput());
bw_ufa.close();
} catch (IOException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
Also used :
TreeRangeSet(com.google.common.collect.TreeRangeSet)
HashMap(java.util.HashMap)
Blast6Segment(cz1.ngs.model.Blast6Segment)
ArrayList(java.util.ArrayList)
BufferedWriter(java.io.BufferedWriter)
ArrayList(java.util.ArrayList)
List(java.util.List)
HashSet(java.util.HashSet)
Sequence(cz1.ngs.model.Sequence)
IOException(java.io.IOException)
DefaultWeightedEdge(org.jgrapht.graph.DefaultWeightedEdge)
AlignmentSegment(cz1.ngs.model.AlignmentSegment)
BufferedReader(java.io.BufferedReader)
HashMap(java.util.HashMap)
Map(java.util.Map)
BidiMap(org.apache.commons.collections4.BidiMap)
DualHashBidiMap(org.apache.commons.collections4.bidimap.DualHashBidiMap)
Example 2 with BFSShortestPath
use of cz1.ngs.model.BFSShortestPath in project polyGembler by c-zhou.
the class Anchor1 method run.
// private static DijkstraShortestPath<Integer, DefaultWeightedEdge> dijkstra_paths;
@Override
public void run() {
// TODO Auto-generated method stub
long tic = System.nanoTime();
sub_seqs = Sequence.parseFastaFileAsMap(subject_file);
qry_seqs = Sequence.parseFastaFileWithRevCmpAsMap(query_file);
// this.makeAssemblyGraph(query_file);
this.makeAssemblyGraph(query_file);
bfs = new BFSShortestPath<Integer, DefaultWeightedEdge>(assembly_graph);
// dijkstra_paths = new DijkstraShortestPath<Integer, DefaultWeightedEdge>(assembly_graph);
// find 'N/n's in subject/reference sequences
// which could have impact on parsing the blast records
final Map<String, TreeRangeSet<Integer>> sub_gaps = new HashMap<String, TreeRangeSet<Integer>>();
final Map<String, List<Blast6Segment>> anchored_records = new HashMap<String, List<Blast6Segment>>();
for (Map.Entry<String, Sequence> entry : sub_seqs.entrySet()) {
String seq_sn = entry.getKey();
String seq_str = entry.getValue().seq_str();
final TreeRangeSet<Integer> tmp_rangeSet = TreeRangeSet.create();
for (int j = 0; j < seq_str.length(); j++) {
if (seq_str.charAt(j) == 'N' || seq_str.charAt(j) == 'n')
// blast record is 1-based closed coordination
tmp_rangeSet.add(Range.closed(j + 1, j + 1).canonical(DiscreteDomain.integers()));
}
int seq_ln = seq_str.length();
final TreeRangeSet<Integer> range_set = TreeRangeSet.create();
for (Range<Integer> range : tmp_rangeSet.asRanges()) {
int lowerend = range.hasLowerBound() ? Math.max(0, range.lowerEndpoint() - gap_buff) : 0;
int upperend = range.hasUpperBound() ? Math.min(seq_ln, range.upperEndpoint() + gap_buff - 1) : seq_ln;
range_set.add(Range.closed(lowerend, upperend).canonical(DiscreteDomain.integers()));
}
sub_gaps.put(seq_sn, range_set);
// initialise an array list for each reference chromosome
anchored_records.put(seq_sn, new ArrayList<Blast6Segment>());
}
// parse blast records
// blast records buffer
final List<Blast6Segment> buff = new ArrayList<Blast6Segment>();
// selected blast records
final List<Blast6Segment> sel_recs = new ArrayList<Blast6Segment>();
// temp list
final List<Blast6Segment> tmp_records = new ArrayList<Blast6Segment>();
try {
BufferedReader br_blast = Utils.getBufferedReader(blast_out);
Blast6Segment tmp_record = Blast6Segment.blast6Segment(br_blast.readLine());
Blast6Segment primary_record, secondary_record;
String qry;
double qry_ln, aln_frac;
while (tmp_record != null) {
qry = tmp_record.qseqid();
qry_ln = qry_seqs.get(qry).seq_ln();
buff.clear();
buff.add(tmp_record);
while ((tmp_record = Blast6Segment.blast6Segment(br_blast.readLine())) != null && tmp_record.qseqid().equals(qry)) {
// filter by identity
if (tmp_record.pident() >= this.min_ident)
buff.add(tmp_record);
}
sel_recs.clear();
// merge collinear records
for (String sub : sub_seqs.keySet()) {
// get all records for subject/reference sequence sub_seq
tmp_records.clear();
for (Blast6Segment record : buff) if (record.sseqid().equals(sub))
tmp_records.add(record);
if (tmp_records.isEmpty())
continue;
// find alignment segments that can be deleted
// those that are subsets of larger alignment segments
// (Sstart, (sstart, send), Send) and (Qstart, (qstart, qend), Qend)
Collections.sort(tmp_records, new Blast6Segment.SegmentSizeComparator());
final Set<int[][]> ranges = new HashSet<int[][]>();
outerloop: for (int i = 0; i < tmp_records.size(); i++) {
primary_record = tmp_records.get(i);
int[][] range = new int[2][2];
if (primary_record.sstart() < primary_record.send()) {
range[0][0] = primary_record.sstart();
range[0][1] = primary_record.send();
} else {
range[0][0] = primary_record.send();
range[0][1] = primary_record.sstart();
}
if (primary_record.qstart() < primary_record.qend()) {
range[1][0] = primary_record.qstart();
range[1][1] = primary_record.qend();
} else {
range[1][0] = primary_record.qend();
range[1][1] = primary_record.qstart();
}
for (int[][] r : ranges) {
if (r[0][0] <= range[0][0] && r[0][1] >= range[0][1] && r[1][0] <= range[1][0] && r[1][1] >= range[1][1]) {
tmp_records.remove(i--);
continue outerloop;
}
}
ranges.add(range);
}
// TODO rewrite this part
/**
* // find collinear alignment segments that can be merged
* Collections.sort(tmp_records, new BlastRecord.SInterceptComparator());
*
* collinear_merged.clear();
* final List<Blast6Record> temp = new ArrayList<Blast6Record>();
* for(int i=0; i<tmp_records.size(); ) {
* Blast6Record record = tmp_records.get(i);
* double max_shift;
* temp.clear();
* temp.add(record);
*
* // find collinear alignment segments
* outerloop:
* while( (++i)<tmp_records.size() ) {
* record = tmp_records.get(i);
* // check if is collinear with other alignment segments
* for(Blast6Record r : temp) {
* max_shift = collinear_shift*
* Math.min(r.length(), record.length());
* if(BlastRecord.sdistance(r, record)<=max_shift &&
* BlastRecord.qdistance(r, record)<=max_shift &&
* BlastRecord.pdistance(r, record)<=max_shift) {
* temp.add(record);
* continue outerloop;
* }
* }
* break;
* }
*
* // merge collinear alignment segments
* int qstart = Integer.MAX_VALUE;
* int qend = Integer.MIN_VALUE;
* int sstart = Integer.MAX_VALUE;
* int send = Integer.MIN_VALUE;
* double pident = 0;
* int length = 0;
*
* // qstart is always smaller than qend
* for(int j=0; j<temp.size(); j++) {
* record = temp.get(j);
* if(record.qstart()<qstart) {
* qstart = record.qstart();
* sstart = record.sstart();
* }
* if(record.qend()>qend) {
* qend = record.qend();
* send = record.send();
* }
* if(record.pident()>pident)
* pident = record.pident();
* length += record.length();
* }
*
* collinear_merged.add(new Blast6Record(qry,sub,pident,length,-1,-1,qstart,qend,sstart,send,-1,-1));
* }
*/
// find collinear alignment segments that can be merged
// more accurate but slower
Collections.sort(tmp_records, new AlignmentSegment.SubjectCoordinationComparator());
Blast6Segment record;
for (int i = 0; i < tmp_records.size(); i++) {
primary_record = tmp_records.get(i);
for (int j = i + 1; j < tmp_records.size(); j++) {
secondary_record = tmp_records.get(j);
double max_shift = collinear_shift * Math.min(primary_record.length(), secondary_record.length());
if ((record = Blast6Segment.collinear(primary_record, secondary_record, max_shift)) != null) {
tmp_records.set(i, record);
tmp_records.remove(j);
--i;
break;
}
}
}
// process blast records that clipped by gaps
// (sstart, send)---(start2, send2)
// (sstart ... --- ... send2)
TreeRangeSet<Integer> sub_gap = sub_gaps.get(sub);
Collections.sort(tmp_records, new AlignmentSegment.SubjectCoordinationComparator());
for (int i = 0; i < tmp_records.size(); i++) {
primary_record = tmp_records.get(i);
if (sub_gap.contains(primary_record.true_send())) {
secondary_record = null;
int sec_j = -1;
for (int j = i + 1; j < tmp_records.size(); j++) {
if (tmp_records.get(j).true_sstart() >= primary_record.true_send()) {
secondary_record = tmp_records.get(j);
sec_j = j;
break;
}
}
if (secondary_record == null || AlignmentSegment.reverse(primary_record, secondary_record)) {
// reverse alignment segments
continue;
}
if (sub_gap.contains(secondary_record.true_sstart()) && sub_gap.rangeContaining(primary_record.true_send()).equals(sub_gap.rangeContaining(secondary_record.true_sstart()))) {
// clipping
// merge two alignment segments
double pident = Math.max(primary_record.pident(), secondary_record.pident());
int qstart = Math.min(primary_record.true_qstart(), secondary_record.true_qstart());
int qend = Math.max(primary_record.true_qend(), secondary_record.true_qend());
int sstart = Math.min(primary_record.true_sstart(), secondary_record.true_sstart());
int send = Math.max(primary_record.true_send(), secondary_record.true_send());
int length = qend - qstart + 1;
// replace primary record with merged record
// delete secondary record
Blast6Segment merged_record = primary_record.forward() ? new Blast6Segment(qry, sub, pident, length, -1, -1, qstart, qend, sstart, send, -1, -1) : new Blast6Segment(qry, sub, pident, length, -1, -1, qstart, qend, send, sstart, -1, -1);
tmp_records.set(i, merged_record);
tmp_records.remove(sec_j);
// the merged records need to be processed
--i;
}
}
}
// add to sel_recs
sel_recs.addAll(tmp_records);
}
// filter by alignment fraction
buff.clear();
buff.addAll(sel_recs);
sel_recs.clear();
for (Blast6Segment record : buff) {
if (record.length() / qry_ln >= this.min_frac)
sel_recs.add(record);
}
if (sel_recs.isEmpty()) {
// continue
continue;
}
// filter blast records
Collections.sort(sel_recs, new Blast6Segment.MatchIndentityComparator());
// process primary alignment
primary_record = sel_recs.get(0);
anchored_records.get(primary_record.sseqid()).add(primary_record);
aln_frac = primary_record.length() / qry_ln;
// and process
for (int i = 1; i < sel_recs.size(); i++) {
secondary_record = sel_recs.get(i);
if (secondary_record.pident() + this.diff_ident < primary_record.pident() || secondary_record.length() / qry_ln + this.diff_frac < aln_frac) {
break;
} else {
anchored_records.get(secondary_record.sseqid()).add(secondary_record);
}
}
}
br_blast.close();
for (Map.Entry<String, List<Blast6Segment>> entry : anchored_records.entrySet()) {
System.out.println(entry.getKey() + ": " + entry.getValue().size());
}
final BufferedWriter bw_map = Utils.getBufferedWriter(out_prefix + ".map");
final BufferedWriter bw_fa = Utils.getBufferedWriter(out_prefix + ".fa");
final Set<String> anchored_seqs = new HashSet<String>();
final List<String> sub_list = Sequence.parseSeqList(subject_file);
final List<GraphPath<Integer, DefaultWeightedEdge>> paths = new ArrayList<GraphPath<Integer, DefaultWeightedEdge>>();
for (String sub_sn : sub_list) {
blast6_records = anchored_records.get(sub_sn);
int nV = blast6_records.size(), count = 0;
// sort blast records
Collections.sort(blast6_records, new Blast6Segment.SubjectCoordinationComparator());
// consensus
int posUpto = 0, send_clip = 0;
int sstart, send, qstart, qend, qlen, tmp_int, qstart_clip, qend_clip, gap_size;
// distance to last 'N', start from next position to find longest common suffix-prefix
int prev_n = Integer.MAX_VALUE;
int mol_len = 0;
int bfs_dist;
String qseq;
int nS, nQ;
// first step: construct super scaffold
// will form a tree graph indicating the path through the contigs
// convert contig names to integer indices
// one contig could end up with multiple indices due to repeats
Map<Integer, String> ss_coordinate = new HashMap<Integer, String>();
int index = 0;
for (Blast6Segment record : blast6_records) ss_coordinate.put(index++, record.qseqid() + (record.forward() ? "" : "'"));
int qseqid1, qseqid2;
String os;
List<Double> dist_stats = new ArrayList<Double>();
for (int i = 0; i != nV; i++) {
qseqid1 = contig_coordinate.getKey(ss_coordinate.get(i));
os = blast6_records.get(i).qseqid() + (blast6_records.get(i).forward() ? "" : "'");
System.out.print(Utils.fixedLengthPaddingString(os, 40) + "\t");
double distance = Double.MAX_VALUE;
for (int j = 1; j <= window_size; j++) {
if (i + j >= nV) {
System.out.print(Utils.fixedLengthPaddingString("", 10) + ",\t");
continue;
}
qseqid2 = contig_coordinate.getKey(ss_coordinate.get(i + j));
GraphPath<Integer, DefaultWeightedEdge> e = bfs.getPath(qseqid1, qseqid2);
// GraphPath<Integer, DefaultWeightedEdge> e = dijkstra_paths.getPath( qseqid1, qseqid2 );
if (e != null) {
double d = e.getLength();
if (distance > d)
distance = d;
}
int l = 0;
if (e != null) {
List<Integer> vertices = e.getVertexList();
for (int k = 1; k < vertices.size() - 1; k++) l += qry_seqs.get(contig_coordinate.get(vertices.get(k))).seq_ln();
l -= (vertices.size() - 2) * this.kmer_size;
}
System.out.print((e == null ? Utils.fixedLengthPaddingString("Inf " + l, 10) + ",\t" : Utils.fixedLengthPaddingString(e.getLength() + " " + l, 10) + ",\t"));
}
System.out.println();
dist_stats.add(distance);
}
double[] dist_arr = ArrayUtils.toPrimitive(dist_stats.toArray(new Double[dist_stats.size()]));
Arrays.sort(dist_arr);
Percentile percentile = new Percentile();
double q1 = percentile.evaluate(dist_arr, 25);
double q3 = percentile.evaluate(dist_arr, 75);
// upper bound for outliers
// q3+1.5*IQR
double outlier = q3 + 1.5 * (q3 - q1);
System.out.println("Outlier upper bound: " + outlier);
// construct a tree graph
pseudo_tree = new DirectedWeightedPseudograph<Integer, DefaultWeightedEdge>(DefaultWeightedEdge.class);
for (int i : ss_coordinate.keySet()) pseudo_tree.addVertex(i);
Blast6Segment record1, record2;
// select the nearest neighbour(s)
for (int i = 0; i != nV; i++) {
System.out.println(i);
record1 = blast6_records.get(i);
send = record1.true_send();
qseqid1 = contig_coordinate.getKey(ss_coordinate.get(i));
paths.clear();
bfs_dist = Integer.MAX_VALUE;
for (int j = i + 1; j < nV; j++) {
record2 = blast6_records.get(j);
sstart = record2.true_sstart();
if (send < sstart)
break;
qseqid2 = contig_coordinate.getKey(ss_coordinate.get(j));
GraphPath<Integer, DefaultWeightedEdge> e = bfs.getPath(qseqid1, qseqid2);
// GraphPath<Integer, DefaultWeightedEdge> e = dijkstra_paths.getPath( qseqid1, qseqid2 );
if (e != null) {
tmp_int = e.getLength();
if (tmp_int == 0)
continue;
if (tmp_int < bfs_dist)
paths.clear();
paths.add(e);
bfs_dist = tmp_int;
}
}
if (paths.isEmpty())
continue;
for (GraphPath<Integer, DefaultWeightedEdge> path : paths) pseudo_tree.setEdgeWeight(pseudo_tree.addEdge(path.getStartVertex(), path.getEndVertex()), 1d);
}
// secondly fill gaps
long toc = System.nanoTime();
System.out.println(toc - tic + " nano secs.");
if (true)
throw new RuntimeException("!!!");
// second step: construct pseudo chromosomes
// 1. choose the first contig
int firstv = 0;
while (true) {
// int v2 = next(firstv+1);
if (true)
break;
}
// 2. extend pseudo chromosomes
StringBuilder seq_str = new StringBuilder();
for (int v = firstv; v < nV - 1; v++) {
if (++count % 10000 == 0)
myLogger.info(sub_sn + " " + count + "/" + nV + " done.");
Blast6Segment record = blast6_records.get(v);
qlen = qry_seqs.get(record.qseqid()).seq_ln();
sstart = record.sstart();
send = record.send();
qstart = record.qstart();
qend = record.qend();
if (sstart > send) {
// make sure sstart<send
tmp_int = sstart;
sstart = send;
send = tmp_int;
tmp_int = qstart;
qstart = qend;
qend = tmp_int;
}
if (qstart > qend) {
qstart_clip = qlen - qstart;
qend_clip = qend - 1;
qseq = Sequence.revCompSeq(qry_seqs.get(record.qseqid()).seq_str());
} else {
qstart_clip = qstart - 1;
qend_clip = qlen - qend;
qseq = qry_seqs.get(record.qseqid()).seq_str();
}
if (send < posUpto || sstart < posUpto && qstart_clip > max_clip) {
// TODO process
continue;
}
// find longest suffix-prefix
nS = seq_str.length();
nQ = qseq.length();
int nO = Math.min(prev_n, Math.min(nS, nQ));
outerloop: for (; nO >= min_overlap; nO--) {
int nS_i = nS - nO;
for (int i = 0; i < nO; i++) {
if (seq_str.charAt(nS_i + i) != qseq.charAt(i))
continue outerloop;
}
break outerloop;
}
if (nO < min_overlap) {
// otherwise will insert a large GAP max(pseduo_distance, max_gap)
if (posUpto > 0) {
if (sstart <= posUpto) {
// if too much overlap, then treat it as a contradiction
if (posUpto - sstart > min_overlap) {
// discard
continue;
} else {
// insert a min_gap
gap_size = min_gap;
}
} else {
// estimate gap size
gap_size = (sstart - posUpto) - (send_clip + qstart_clip);
if (gap_size < max_gap)
gap_size = max_gap;
}
bw_map.write("GAP\t" + gap_size + "\t0\t" + gap_size + "\t+\t" + sub_sn + "\t" + mol_len + "\t" + (mol_len + gap_size) + "\n");
seq_str.append(Sequence.polyN(gap_size));
mol_len += gap_size;
}
bw_map.write(record.qseqid() + "\t" + qlen + "\t");
if (qstart > qend) {
// reverse
bw_map.write("0\t" + qlen + "\t-\t");
} else {
// forward
bw_map.write("0\t" + qlen + "\t+\t");
}
seq_str.append(qseq);
bw_map.write(sub_sn + "\t" + mol_len + "\t" + (mol_len + qlen) + "\n");
mol_len += qlen;
prev_n = qlen;
anchored_seqs.add(record.qseqid());
} else {
// overlap found
// will not insert gap
// ====================
// /-------\
// /----\
// calculate overlaps
// process overlap
qstart = nO;
if (qstart == qlen)
continue;
bw_map.write(record.qseqid() + "\t" + (qlen - qstart) + "\t");
if (qstart > qend) {
// reverse
bw_map.write(0 + "\t" + (qlen - qstart) + "\t-\t");
} else {
// forward
bw_map.write(qstart + "\t" + qlen + "\t+\t");
}
bw_map.write(sub_sn + "\t" + mol_len + "\t" + (mol_len + qlen - qstart) + "\n");
mol_len += qlen - qstart;
prev_n += qlen - qstart;
seq_str.append(qseq.substring(qstart));
anchored_seqs.add(record.qseqid());
}
posUpto = send;
send_clip = qend_clip;
}
if (seq_str.length() > 0)
bw_fa.write(Sequence.formatOutput(sub_sn, seq_str.toString()));
}
bw_fa.close();
bw_map.close();
final BufferedWriter bw_ufa = Utils.getBufferedWriter(out_prefix + "_unplaced.fa");
for (String seq : qry_seqs.keySet()) if (!anchored_seqs.contains(seq))
bw_ufa.write(qry_seqs.get(seq).formatOutput());
bw_ufa.close();
} catch (IOException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
Also used :
Percentile(org.apache.commons.math3.stat.descriptive.rank.Percentile)
TreeRangeSet(com.google.common.collect.TreeRangeSet)
HashMap(java.util.HashMap)
Blast6Segment(cz1.ngs.model.Blast6Segment)
ArrayList(java.util.ArrayList)
GraphPath(org.jgrapht.GraphPath)
BufferedWriter(java.io.BufferedWriter)
ArrayList(java.util.ArrayList)
List(java.util.List)
HashSet(java.util.HashSet)
Sequence(cz1.ngs.model.Sequence)
IOException(java.io.IOException)
DefaultWeightedEdge(org.jgrapht.graph.DefaultWeightedEdge)
AlignmentSegment(cz1.ngs.model.AlignmentSegment)
BufferedReader(java.io.BufferedReader)
HashMap(java.util.HashMap)
Map(java.util.Map)
BidiMap(org.apache.commons.collections4.BidiMap)
DualHashBidiMap(org.apache.commons.collections4.bidimap.DualHashBidiMap)
Example 3 with BFSShortestPath
use of cz1.ngs.model.BFSShortestPath in project polyGembler by c-zhou.
the class AssemblyGraph method validateAssemblyGraph.
private static void validateAssemblyGraph(String query_file, String bam_in) {
final SamReaderFactory factory = SamReaderFactory.makeDefault().enable(SamReaderFactory.Option.INCLUDE_SOURCE_IN_RECORDS, SamReaderFactory.Option.VALIDATE_CRC_CHECKSUMS).validationStringency(ValidationStringency.SILENT);
qry_seqs = Sequence.parseFastaFileWithRevCmpAsMap(query_file);
final SamReader in1 = factory.open(new File(bam_in));
SAMRecordIterator iter1 = in1.iterator();
final List<SAMRecord> buffered_records = new ArrayList<SAMRecord>();
SAMRecord tmp_record1 = iter1.hasNext() ? iter1.next() : null;
String record_id = tmp_record1.getReadName();
SAMRecord r1, r2;
int i1, i2;
StringBuilder oos = new StringBuilder();
String source, sink;
final BFSShortestPath<Integer, DefaultWeightedEdge> bfs = new BFSShortestPath<Integer, DefaultWeightedEdge>(assembly_graph);
while (tmp_record1 != null) {
buffered_records.clear();
buffered_records.add(tmp_record1);
while ((tmp_record1 = iter1.hasNext() ? iter1.next() : null) != null && tmp_record1.getReadName().equals(record_id)) {
buffered_records.add(tmp_record1);
}
if (buffered_records.size() == 1) {
if (tmp_record1 != null)
record_id = tmp_record1.getReadName();
continue;
}
// sort records by head clipping size
buffered_records.sort(new Comparator<SAMRecord>() {
@Override
public int compare(SAMRecord r0, SAMRecord r1) {
// TODO Auto-generated method stub
return Integer.compare(head_clip(r0), head_clip(r1));
}
private int head_clip(SAMRecord r) {
CigarElement cigar = r.getReadNegativeStrandFlag() ? r.getCigar().getLastCigarElement() : r.getCigar().getFirstCigarElement();
return cigar.getOperator().isClipping() ? cigar.getLength() : 0;
}
});
// calculate distance for adjacent alignment record pairs
r1 = buffered_records.get(0);
// for writing
oos.setLength(0);
oos.append(record_id);
oos.append("[");
oos.append(buffered_records.size());
oos.append(", ");
oos.append(readLength(r1));
// find reference index
source = r1.getReferenceName() + (r1.getReadNegativeStrandFlag() ? "'" : "");
i1 = contig_coordinate.getKey(source);
oos.append(", ");
oos.append(source);
oos.append("] ");
for (int i = 1; i < buffered_records.size(); i++) {
r2 = buffered_records.get(i);
sink = r2.getReferenceName() + (r2.getReadNegativeStrandFlag() ? "'" : "");
i2 = contig_coordinate.getKey(sink);
oos.append(", (");
GraphPath<Integer, DefaultWeightedEdge> e = bfs.getPath(i1, i2);
oos.append(sink);
oos.append(",");
oos.append(e == null ? "Inf" : e.getLength());
oos.append(",");
oos.append(pathLength(e));
oos.append(")");
i1 = i2;
}
oos.append("\n");
System.out.println(oos.toString());
if (tmp_record1 != null)
record_id = tmp_record1.getReadName();
}
try {
iter1.close();
in1.close();
} catch (IOException e) {
// TODO Auto-generated catch block
e.printStackTrace();
}
}
Also used :
SamReaderFactory(htsjdk.samtools.SamReaderFactory)
SAMRecordIterator(htsjdk.samtools.SAMRecordIterator)
ArrayList(java.util.ArrayList)
IOException(java.io.IOException)
CigarElement(htsjdk.samtools.CigarElement)
DefaultWeightedEdge(org.jgrapht.graph.DefaultWeightedEdge)
SamReader(htsjdk.samtools.SamReader)
SAMRecord(htsjdk.samtools.SAMRecord)
BFSShortestPath(cz1.ngs.model.BFSShortestPath)
File(java.io.File)
Aggregations
IOException (java.io.IOException)3
ArrayList (java.util.ArrayList)3
DefaultWeightedEdge (org.jgrapht.graph.DefaultWeightedEdge)3
TreeRangeSet (com.google.common.collect.TreeRangeSet)2
AlignmentSegment (cz1.ngs.model.AlignmentSegment)2
Blast6Segment (cz1.ngs.model.Blast6Segment)2
Sequence (cz1.ngs.model.Sequence)2
BufferedReader (java.io.BufferedReader)2
BufferedWriter (java.io.BufferedWriter)2
HashMap (java.util.HashMap)2
HashSet (java.util.HashSet)2
List (java.util.List)2
Map (java.util.Map)2
BidiMap (org.apache.commons.collections4.BidiMap)2
DualHashBidiMap (org.apache.commons.collections4.bidimap.DualHashBidiMap)2
BFSShortestPath (cz1.ngs.model.BFSShortestPath)1
CigarElement (htsjdk.samtools.CigarElement)1
SAMRecord (htsjdk.samtools.SAMRecord)1
SAMRecordIterator (htsjdk.samtools.SAMRecordIterator)1
SamReader (htsjdk.samtools.SamReader)1
