use of gdsc.smlm.model.FluorophoreSequenceModel in project GDSC-SMLM by aherbert.
the class BlinkEstimatorTest method estimateBlinking.
private TIntHashSet estimateBlinking(double nBlinks, double tOn, double tOff, int particles, double fixedFraction, boolean timeAtLowerBound, boolean doAssert) {
SpatialIllumination activationIllumination = new UniformIllumination(100);
int totalSteps = 100;
double eAct = totalSteps * 0.3 * activationIllumination.getAveragePhotons();
ImageModel imageModel = new ActivationEnergyImageModel(eAct, activationIllumination, tOn, 0, tOff, 0, nBlinks);
imageModel.setRandomGenerator(rand);
double[] max = new double[] { 256, 256, 32 };
double[] min = new double[3];
SpatialDistribution distribution = new UniformDistribution(min, max, rand.nextInt());
List<CompoundMoleculeModel> compounds = new ArrayList<CompoundMoleculeModel>(1);
CompoundMoleculeModel c = new CompoundMoleculeModel(1, 0, 0, 0, Arrays.asList(new MoleculeModel(0, 0, 0, 0)));
c.setDiffusionRate(diffusionRate);
c.setDiffusionType(DiffusionType.RANDOM_WALK);
compounds.add(c);
List<CompoundMoleculeModel> molecules = imageModel.createMolecules(compounds, particles, distribution, false);
// Activate fluorophores
List<? extends FluorophoreSequenceModel> fluorophores = imageModel.createFluorophores(molecules, totalSteps);
totalSteps = checkTotalSteps(totalSteps, fluorophores);
List<LocalisationModel> localisations = imageModel.createImage(molecules, fixedFraction, totalSteps, photons, 0.5, false);
// // Remove localisations to simulate missed counts.
// List<LocalisationModel> newLocalisations = new ArrayList<LocalisationModel>(localisations.size());
// boolean[] id = new boolean[fluorophores.size() + 1];
// Statistics photonStats = new Statistics();
// for (LocalisationModel l : localisations)
// {
// photonStats.add(l.getIntensity());
// // Remove by intensity threshold and optionally at random.
// if (l.getIntensity() < minPhotons || rand.nextDouble() < pDelete)
// continue;
// newLocalisations.add(l);
// id[l.getId()] = true;
// }
// localisations = newLocalisations;
// System.out.printf("Photons = %f\n", photonStats.getMean());
//
// List<FluorophoreSequenceModel> newFluorophores = new ArrayList<FluorophoreSequenceModel>(fluorophores.size());
// for (FluorophoreSequenceModel f : fluorophores)
// {
// if (id[f.getId()])
// newFluorophores.add(f);
// }
// fluorophores = newFluorophores;
MemoryPeakResults results = new MemoryPeakResults();
results.setCalibration(new Calibration(pixelPitch, 1, msPerFrame));
for (LocalisationModel l : localisations) {
// Remove by intensity threshold and optionally at random.
if (l.getIntensity() < minPhotons || rand.nextDouble() < pDelete)
continue;
float[] params = new float[7];
params[Gaussian2DFunction.X_POSITION] = (float) l.getX();
params[Gaussian2DFunction.Y_POSITION] = (float) l.getY();
params[Gaussian2DFunction.X_SD] = params[Gaussian2DFunction.Y_SD] = psfWidth;
params[Gaussian2DFunction.SIGNAL] = (float) (l.getIntensity());
results.addf(l.getTime(), 0, 0, 0, 0, 0, params, null);
}
// Add random localisations
for (int i = (int) (localisations.size() * pAdd); i-- > 0; ) {
float[] params = new float[7];
params[Gaussian2DFunction.X_POSITION] = (float) (rand.nextDouble() * max[0]);
params[Gaussian2DFunction.Y_POSITION] = (float) (rand.nextDouble() * max[1]);
params[Gaussian2DFunction.X_SD] = params[Gaussian2DFunction.Y_SD] = psfWidth;
// Intensity doesn't matter at the moment for tracing
params[Gaussian2DFunction.SIGNAL] = (float) (photons);
results.addf(1 + rand.nextInt(totalSteps), 0, 0, 0, 0, 0, params, null);
}
// Get actual simulated stats ...
Statistics statsNBlinks = new Statistics();
Statistics statsTOn = new Statistics();
Statistics statsTOff = new Statistics();
Statistics statsSampledNBlinks = new Statistics();
Statistics statsSampledTOn = new Statistics();
StoredDataStatistics statsSampledTOff = new StoredDataStatistics();
for (FluorophoreSequenceModel f : fluorophores) {
statsNBlinks.add(f.getNumberOfBlinks());
statsTOn.add(f.getOnTimes());
statsTOff.add(f.getOffTimes());
int[] on = f.getSampledOnTimes();
statsSampledNBlinks.add(on.length);
statsSampledTOn.add(on);
statsSampledTOff.add(f.getSampledOffTimes());
}
System.out.printf("N = %d (%d), N-blinks = %f, tOn = %f, tOff = %f, Fixed = %f\n", fluorophores.size(), localisations.size(), nBlinks, tOn, tOff, fixedFraction);
System.out.printf("Actual N-blinks = %f (%f), tOn = %f (%f), tOff = %f (%f), 95%% = %f, max = %f\n", statsNBlinks.getMean(), statsSampledNBlinks.getMean(), statsTOn.getMean(), statsSampledTOn.getMean(), statsTOff.getMean(), statsSampledTOff.getMean(), statsSampledTOff.getStatistics().getPercentile(95), statsSampledTOff.getStatistics().getMax());
System.out.printf("-=-=--=-\n");
BlinkEstimator be = new BlinkEstimator();
be.maxDarkTime = (int) (tOff * 10);
be.msPerFrame = msPerFrame;
be.relativeDistance = false;
double d = ImageModel.getRandomMoveDistance(diffusionRate);
be.searchDistance = (fixedFraction < 1) ? Math.sqrt(2 * d * d) * 3 : 0;
be.timeAtLowerBound = timeAtLowerBound;
be.showPlots = false;
//Assert.assertTrue("Max dark time must exceed the dark time of the data (otherwise no plateau)",
// be.maxDarkTime > statsSampledTOff.getStatistics().getMax());
int nMolecules = fluorophores.size();
if (usePopulationStatistics) {
nBlinks = statsNBlinks.getMean();
tOff = statsTOff.getMean();
} else {
nBlinks = statsSampledNBlinks.getMean();
tOff = statsSampledTOff.getMean();
}
// See if any fitting regime gets a correct answer
TIntHashSet ok = new TIntHashSet();
for (int nFittedPoints = MIN_FITTED_POINTS; nFittedPoints <= MAX_FITTED_POINTS; nFittedPoints++) {
be.nFittedPoints = nFittedPoints;
be.computeBlinkingRate(results, true);
double moleculesError = DoubleEquality.relativeError(nMolecules, be.getNMolecules());
double blinksError = DoubleEquality.relativeError(nBlinks, be.getNBlinks());
double offError = DoubleEquality.relativeError(tOff * msPerFrame, be.getTOff());
System.out.printf("Error %d: N = %f, blinks = %f, tOff = %f : %f\n", nFittedPoints, moleculesError, blinksError, offError, (moleculesError + blinksError + offError) / 3);
if (moleculesError < relativeError && blinksError < relativeError && offError < relativeError) {
ok.add(nFittedPoints);
System.out.printf("-=-=--=-\n");
System.out.printf("*** Correct at %d fitted points ***\n", nFittedPoints);
if (doAssert)
break;
}
//if (!be.isIncreaseNFittedPoints())
// break;
}
System.out.printf("-=-=--=-\n");
if (doAssert)
Assert.assertFalse(ok.isEmpty());
//Assert.assertEquals("Invalid t-off", tOff * msPerFrame, be.getTOff(), tOff * msPerFrame * relativeError);
return ok;
}
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