Examples with ImageWindow ij.gui.ImageWindow used on opensource projects

Example 1 with ImageWindow

use of ij.gui.ImageWindow in project GDSC-SMLM by aherbert.

the class DrawClusters method run.

/*
	 * (non-Javadoc)
	 * 
	 * @see ij.plugin.PlugIn#run(java.lang.String)
	 */
public void run(String arg) {
    SMLMUsageTracker.recordPlugin(this.getClass(), arg);
    if (MemoryPeakResults.isMemoryEmpty()) {
        IJ.error(TITLE, "No localisations in memory");
        return;
    }
    if (!showDialog())
        return;
    // Load the results
    MemoryPeakResults results = ResultsManager.loadInputResults(inputOption, false);
    if (results == null || results.size() == 0) {
        IJ.error(TITLE, "No results could be loaded");
        return;
    }
    // Get the traces
    Trace[] traces = TraceManager.convert(results);
    if (traces == null || traces.length == 0) {
        IJ.error(TITLE, "No traces could be loaded");
        return;
    }
    // Filter traces to a min size
    int maxFrame = 0;
    int count = 0;
    final int myMaxSize = (maxSize < minSize) ? Integer.MAX_VALUE : maxSize;
    final boolean myDrawLines = (myMaxSize < 2) ? false : drawLines;
    for (int i = 0; i < traces.length; i++) {
        if (expandToSingles)
            traces[i].expandToSingles();
        if (traces[i].size() >= minSize && traces[i].size() <= myMaxSize) {
            traces[count++] = traces[i];
            traces[i].sort();
            if (maxFrame < traces[i].getTail().getFrame())
                maxFrame = traces[i].getTail().getFrame();
        }
    }
    if (count == 0) {
        IJ.error(TITLE, "No traces achieved the size limits");
        return;
    }
    String msg = String.format(TITLE + ": %d / %s (%s)", count, Utils.pleural(traces.length, "trace"), Utils.pleural(results.size(), "localisation"));
    IJ.showStatus(msg);
    //Utils.log(msg);
    Rectangle bounds = results.getBounds(true);
    ImagePlus imp = WindowManager.getImage(title);
    boolean isUseStackPosition = useStackPosition;
    if (imp == null) {
        // Create a default image using 100 pixels as the longest edge
        double maxD = (bounds.width > bounds.height) ? bounds.width : bounds.height;
        int w, h;
        if (maxD == 0) {
            // Note that imageSize can be zero (for auto sizing)
            w = h = (imageSize == 0) ? 20 : imageSize;
        } else {
            // Note that imageSize can be zero (for auto sizing)
            if (imageSize == 0) {
                w = bounds.width;
                h = bounds.height;
            } else {
                w = (int) (imageSize * bounds.width / maxD);
                h = (int) (imageSize * bounds.height / maxD);
            }
        }
        ByteProcessor bp = new ByteProcessor(w, h);
        if (isUseStackPosition) {
            ImageStack stack = new ImageStack(w, h, maxFrame);
            for (int i = 1; i <= maxFrame; i++) // Do not clone as the image is empty
            stack.setPixels(bp.getPixels(), i);
            imp = Utils.display(TITLE, stack);
        } else
            imp = Utils.display(TITLE, bp);
        // Enlarge
        ImageWindow iw = imp.getWindow();
        for (int i = 9; i-- > 0 && iw.getWidth() < 500 && iw.getHeight() < 500; ) {
            iw.getCanvas().zoomIn(imp.getWidth() / 2, imp.getHeight() / 2);
        }
    } else {
        // Check if the image has enough frames for all the traces
        if (maxFrame > imp.getNFrames())
            isUseStackPosition = false;
    }
    final float xScale = (float) (imp.getWidth() / bounds.getWidth());
    final float yScale = (float) (imp.getHeight() / bounds.getHeight());
    // Create ROIs and store data to sort them
    Roi[] rois = new Roi[count];
    int[][] frames = (isUseStackPosition) ? new int[count][] : null;
    int[] indices = Utils.newArray(count, 0, 1);
    double[] values = new double[count];
    for (int i = 0; i < count; i++) {
        Trace trace = traces[i];
        int nPoints = trace.size();
        float[] xPoints = new float[nPoints];
        float[] yPoints = new float[nPoints];
        int j = 0;
        if (isUseStackPosition)
            frames[i] = new int[nPoints];
        for (PeakResult result : trace.getPoints()) {
            xPoints[j] = (result.getXPosition() - bounds.x) * xScale;
            yPoints[j] = (result.getYPosition() - bounds.y) * yScale;
            if (isUseStackPosition)
                frames[i][j] = result.getFrame();
            j++;
        }
        Roi roi;
        if (myDrawLines) {
            roi = new PolygonRoi(xPoints, yPoints, nPoints, Roi.POLYLINE);
            if (splineFit)
                ((PolygonRoi) roi).fitSpline();
        } else {
            roi = new PointRoi(xPoints, yPoints, nPoints);
            ((PointRoi) roi).setShowLabels(false);
        }
        rois[i] = roi;
        switch(sort) {
            case 0:
            default:
                break;
            case // Sort by ID
            1:
                values[i] = traces[i].getId();
                break;
            case // Sort by time
            2:
                values[i] = traces[i].getHead().getFrame();
                break;
            case // Sort by size descending
            3:
                values[i] = -traces[i].size();
                break;
            case // Sort by length descending
            4:
                values[i] = -roi.getLength();
                break;
            case // Mean Square Displacement
            5:
                values[i] = -traces[i].getMSD();
                break;
            case // Mean / Frame
            6:
                values[i] = -traces[i].getMeanPerFrame();
                break;
        }
    }
    if (sort > 0)
        Sort.sort(indices, values);
    // Draw the traces as ROIs on an overlay
    Overlay o = new Overlay();
    LUT lut = LUTHelper.createLUT(DrawClusters.lut);
    final double scale = 256.0 / count;
    if (isUseStackPosition) {
        // Add the tracks on the frames containing the results
        final boolean isHyperStack = imp.isDisplayedHyperStack();
        for (int i = 0; i < count; i++) {
            final int index = indices[i];
            final Color c = LUTHelper.getColour(lut, (int) (i * scale));
            final PolygonRoi roi = (PolygonRoi) rois[index];
            roi.setFillColor(c);
            roi.setStrokeColor(c);
            final FloatPolygon fp = roi.getNonSplineFloatPolygon();
            // For each frame in the track, add the ROI track and a point ROI for the current position
            for (int j = 0; j < frames[index].length; j++) {
                addToOverlay(o, (Roi) roi.clone(), isHyperStack, frames[index][j]);
                //PointRoi pointRoi = new PointRoi(pos.x + fp.xpoints[j], pos.y + fp.ypoints[j]);
                PointRoi pointRoi = new PointRoi(fp.xpoints[j], fp.ypoints[j]);
                pointRoi.setPointType(3);
                pointRoi.setFillColor(c);
                pointRoi.setStrokeColor(Color.black);
                addToOverlay(o, pointRoi, isHyperStack, frames[index][j]);
            }
        }
    } else {
        // Add the tracks as a single overlay
        for (int i = 0; i < count; i++) {
            final Roi roi = rois[indices[i]];
            roi.setStrokeColor(new Color(lut.getRGB((int) (i * scale))));
            o.add(roi);
        }
    }
    imp.setOverlay(o);
    IJ.showStatus(msg);
}

Example 2 with ImageWindow

use of ij.gui.ImageWindow in project GDSC-SMLM by aherbert.

the class BenchmarkFilterAnalysis method saveTemplate.

/**
	 * Save PeakFit configuration template using the current benchmark settings.
	 * 
	 * @param topFilterSummary
	 */
private void saveTemplate(String topFilterSummary) {
    FitEngineConfiguration config = new FitEngineConfiguration(new FitConfiguration());
    if (!updateAllConfiguration(config, true)) {
        IJ.log("Unable to create the template configuration");
        return;
    }
    // Remove the PSF width to make the template generic
    config.getFitConfiguration().setInitialPeakStdDev(0);
    String filename = getFilename("Template_File", templateFilename);
    if (filename != null) {
        templateFilename = filename;
        Prefs.set(KEY_TEMPLATE_FILENAME, filename);
        GlobalSettings settings = new GlobalSettings();
        settings.setNotes(getNotes(topFilterSummary));
        settings.setFitEngineConfiguration(config);
        if (!SettingsManager.saveSettings(settings, filename, true)) {
            IJ.log("Unable to save the template configuration");
            return;
        }
        // Save some random frames from the test image data
        ImagePlus imp = CreateData.getImage();
        if (imp == null)
            return;
        // Get the number of frames
        final ImageStack stack = imp.getImageStack();
        if (sampler == null || sampler.getResults() != results) {
            sampler = new ResultsImageSampler(results, stack, 32);
            sampler.analyse();
        }
        if (!sampler.isValid())
            return;
        // Iteratively show the example until the user is happy.
        // Yes = OK, No = Repeat, Cancel = Do not save
        String keyNo = "nNo";
        String keyLow = "nLower";
        String keyHigh = "nHigher";
        if (Utils.isMacro()) {
            // Collect the options if running in a macro
            String options = Macro.getOptions();
            nNo = Integer.parseInt(Macro.getValue(options, keyNo, Integer.toString(nNo)));
            nLow = Integer.parseInt(Macro.getValue(options, keyLow, Integer.toString(nLow)));
            nHigh = Integer.parseInt(Macro.getValue(options, keyHigh, Integer.toString(nHigh)));
        } else {
            if (nLow + nHigh == 0)
                nLow = nHigh = 1;
        }
        final ImagePlus[] out = new ImagePlus[1];
        out[0] = sampler.getSample(nNo, nLow, nHigh);
        if (!Utils.isMacro()) {
            // Show the template results
            final ConfigurationTemplate configTemplate = new ConfigurationTemplate();
            // Interactively show the sample image data
            final boolean[] close = new boolean[1];
            final ImagePlus[] outImp = new ImagePlus[1];
            if (out[0] != null) {
                outImp[0] = display(out[0]);
                if (Utils.isNewWindow()) {
                    close[0] = true;
                    // Zoom a bit
                    ImageWindow iw = outImp[0].getWindow();
                    for (int i = 7; i-- > 0 && Math.max(iw.getWidth(), iw.getHeight()) < 512; ) {
                        iw.getCanvas().zoomIn(0, 0);
                    }
                }
                configTemplate.createResults(outImp[0]);
            }
            // TODO - fix this when a second sample is made as the results are not updated.
            ImageListener listener = new ImageListener() {

                public void imageOpened(ImagePlus imp) {
                }

                public void imageClosed(ImagePlus imp) {
                }

                public void imageUpdated(ImagePlus imp) {
                    if (imp != null && imp == outImp[0]) {
                        configTemplate.updateResults(imp.getCurrentSlice());
                    }
                }
            };
            ImagePlus.addImageListener(listener);
            // For the dialog
            String msg = String.format("Showing image data for the template example.\n \nSample Frames:\nEmpty = %d\nLower density = %d\nHigher density = %d\n", sampler.getNumberOfEmptySamples(), sampler.getNumberOfLowDensitySamples(), sampler.getNumberOfHighDensitySamples());
            // Turn off the recorder when the dialog is showing
            boolean record = Recorder.record;
            Recorder.record = false;
            NonBlockingGenericDialog gd = new NonBlockingGenericDialog(TITLE);
            gd.addMessage(msg);
            //gd.enableYesNoCancel(" Save ", " Resample ");
            gd.addSlider(keyNo, 0, 10, nNo);
            gd.addSlider(keyLow, 0, 10, nLow);
            gd.addSlider(keyHigh, 0, 10, nHigh);
            gd.addDialogListener(new DialogListener() {

                public boolean dialogItemChanged(GenericDialog gd, AWTEvent e) {
                    // image the user has not seen.
                    if (e == null)
                        return true;
                    nNo = (int) gd.getNextNumber();
                    nLow = (int) gd.getNextNumber();
                    nHigh = (int) gd.getNextNumber();
                    out[0] = sampler.getSample(nNo, nLow, nHigh);
                    if (out[0] != null) {
                        outImp[0] = display(out[0]);
                        if (Utils.isNewWindow()) {
                            close[0] = true;
                            // Zoom a bit
                            ImageWindow iw = outImp[0].getWindow();
                            for (int i = 7; i-- > 0 && Math.max(iw.getWidth(), iw.getHeight()) < 512; ) {
                                iw.getCanvas().zoomIn(0, 0);
                            }
                        }
                        configTemplate.createResults(outImp[0]);
                    }
                    return true;
                }
            });
            gd.showDialog();
            if (gd.wasCanceled()) {
                out[0] = null;
                // For the recorder
                nNo = nLow = nHigh = 0;
            }
            if (close[0]) {
                // Because closing the image sets the stack pixels array to null
                if (out[0] != null)
                    out[0] = out[0].duplicate();
                outImp[0].close();
            }
            configTemplate.closeResults();
            ImagePlus.removeImageListener(listener);
            if (record) {
                Recorder.record = true;
                Recorder.recordOption(keyNo, Integer.toString(nNo));
                Recorder.recordOption(keyLow, Integer.toString(nLow));
                Recorder.recordOption(keyHigh, Integer.toString(nHigh));
            }
        }
        if (out[0] == null)
            return;
        ImagePlus example = out[0];
        filename = Utils.replaceExtension(filename, ".tif");
        IJ.save(example, filename);
    }
}

Example 3 with ImageWindow

use of ij.gui.ImageWindow in project GDSC-SMLM by aherbert.

the class ConfigurationTemplate method updateResults.

/**
	 * Update the results window using the current selected slice from the template image.
	 *
	 * @param slice
	 *            the slice
	 * @return the text window
	 */
public TextWindow updateResults(int slice) {
    if (slice == currentSlice || text == null)
        return resultsWindow;
    currentSlice = slice;
    if (resultsWindow == null || !resultsWindow.isVisible()) {
        resultsWindow = new TextWindow(TITLE + " Results", headings, "", 450, 250);
        // Put next to the image
        ImagePlus imp = WindowManager.getImage(templateId);
        if (imp != null && imp.getWindow() != null) {
            ImageWindow iw = imp.getWindow();
            Point p = iw.getLocation();
            p.x += iw.getWidth();
            resultsWindow.setLocation(p);
        }
    }
    resultsWindow.getTextPanel().clear();
    String data = text.get(slice);
    if (!Utils.isNullOrEmpty(data))
        resultsWindow.append(data);
    return resultsWindow;
}

Example 4 with ImageWindow

use of ij.gui.ImageWindow in project GDSC-SMLM by aherbert.

the class ConfigurationTemplate method showTemplateImage.

private void showTemplateImage(String name) {
    ImagePlus imp = getTemplateImage(name);
    if (imp == null) {
        IJ.error(TITLE, "Failed to load example image for template: " + name);
    } else {
        this.imp = displayTemplate(TITLE, imp);
        if (Utils.isNewWindow()) {
            // Zoom a bit
            ImageWindow iw = this.imp.getWindow();
            for (int i = 7; i-- > 0 && Math.max(iw.getWidth(), iw.getHeight()) < 512; ) {
                iw.getCanvas().zoomIn(0, 0);
            }
        }
        createResults(this.imp);
        showTemplateInfo(name);
    }
}