Examples with IterableInterval net.imglib2.IterableInterval used on opensource projects

Example 1 with IterableInterval

use of net.imglib2.IterableInterval in project vcell by virtualcell.

the class ConstructTIRFGeometry method run.

@Override
public void run() {
    // Calculate constant d in TIRF exponential decay function
    // Angle of incidence in radians
    theta = theta * 2 * Math.PI / 360;
    // Refractive index of glass
    final double n1 = 1.52;
    // Refractive index of cytosol
    final double n2 = 1.38;
    final double d = lambda * Math.pow((Math.pow(n1, 2) * Math.pow(Math.sin(theta), 2) - Math.pow(n2, 2)), -0.5) / (4 * Math.PI);
    System.out.println("d: " + d);
    final double fluorPerMolecule = 250;
    // Get frame of interest to define geometry
    long maxX = data.dimension(0) - 1;
    long maxY = data.dimension(1) - 1;
    Interval interval = Intervals.createMinMax(0, 0, sliceIndex, maxX, maxY, sliceIndex);
    RandomAccessibleInterval<T> croppedRAI = ops.transform().crop(data, interval, true);
    // Subtract lowest pixel value
    IterableInterval<T> dataII = Views.iterable(croppedRAI);
    double min = ops.stats().min(dataII).getRealDouble();
    Cursor<T> dataCursor = dataII.cursor();
    while (dataCursor.hasNext()) {
        double val = dataCursor.next().getRealDouble();
        dataCursor.get().setReal(val - min);
    }
    // Perform Gaussian blur
    RandomAccessibleInterval<T> blurredRAI = ops.filter().gauss(croppedRAI, 2);
    IterableInterval<T> blurredII = Views.iterable(blurredRAI);
    // Segment slice by threshold and fill holes
    IterableInterval<BitType> thresholded = ops.threshold().huang(blurredII);
    Img<BitType> thresholdedImg = ops.convert().bit(thresholded);
    RandomAccessibleInterval<BitType> thresholdedRAI = ops.morphology().fillHoles(thresholdedImg);
    // Get the largest region
    RandomAccessibleInterval<LabelingType<ByteType>> labeling = ops.labeling().cca(thresholdedRAI, ConnectedComponents.StructuringElement.EIGHT_CONNECTED);
    LabelRegions<ByteType> labelRegions = new LabelRegions<>(labeling);
    Iterator<LabelRegion<ByteType>> iterator = labelRegions.iterator();
    LabelRegion<ByteType> maxRegion = iterator.next();
    while (iterator.hasNext()) {
        LabelRegion<ByteType> currRegion = iterator.next();
        if (currRegion.size() > maxRegion.size()) {
            maxRegion = currRegion;
        }
    }
    // Generate z index map
    double iMax = ops.stats().max(dataII).getRealDouble();
    Img<UnsignedShortType> dataImg = ops.convert().uint16(dataII);
    Img<UnsignedShortType> zMap = ops.convert().uint16(ops.create().img(dataII));
    LabelRegionCursor cursor = maxRegion.localizingCursor();
    RandomAccess<UnsignedShortType> zMapRA = zMap.randomAccess();
    RandomAccess<UnsignedShortType> dataRA = dataImg.randomAccess();
    while (cursor.hasNext()) {
        cursor.fwd();
        zMapRA.setPosition(cursor);
        dataRA.setPosition(cursor);
        double val = dataRA.get().getRealDouble();
        // Log of 0 is undefined
        if (val < 1) {
            val = 1;
        }
        int z = (int) Math.round(-d * Math.log(val / iMax) / zRes);
        zMapRA.get().set(z);
    }
    System.out.println("6");
    // Use map to construct 3D geometry
    // Add 5 slices of padding on top
    int maxZ = (int) ops.stats().max(zMap).getRealDouble() + 5;
    long[] resultDimensions = { maxX + 1, maxY + 1, maxZ };
    Img<BitType> result = new ArrayImgFactory<BitType>().create(resultDimensions, new BitType());
    RandomAccess<BitType> resultRA = result.randomAccess();
    System.out.println(maxZ);
    cursor.reset();
    while (cursor.hasNext()) {
        cursor.fwd();
        zMapRA.setPosition(cursor);
        int zIndex = zMapRA.get().get();
        int[] position = { cursor.getIntPosition(0), cursor.getIntPosition(1), zIndex };
        while (position[2] < maxZ) {
            resultRA.setPosition(position);
            resultRA.get().set(true);
            position[2]++;
        }
    }
    output = datasetService.create(result);
    CalibratedAxis[] axes = new DefaultLinearAxis[] { new DefaultLinearAxis(Axes.X), new DefaultLinearAxis(Axes.Y), new DefaultLinearAxis(Axes.Z) };
    output.setAxes(axes);
    System.out.println("Done constructing geometry");
}

Example 2 with IterableInterval

use of net.imglib2.IterableInterval in project vcell by virtualcell.

the class ImageStatsForPlotting method computeMean.

/**
 * Computes the mean of each XY slice along the 3rd dimension
 * TODO: Currently assumes only 3 dimensions, must handle time series of z stacks and multiple channels
 * @param data
 * @return Pair containing A) the 3rd dimension index, and B) the mean value of the XY slice
 */
private Pair<double[], double[]> computeMean(RandomAccessibleInterval<T> data, IterableInterval<BitType> mask) {
    double[] indices = new double[(int) data.dimension(2)];
    double[] means = new double[indices.length];
    for (int z = 0; z < indices.length; z++) {
        FinalInterval interval = Intervals.createMinMax(0, 0, z, data.dimension(0) - 1, data.dimension(1) - 1, z);
        double mean = 0.0;
        RandomAccessibleInterval<T> cropped = ops.transform().crop(data, interval);
        if (mask == null) {
            mean = ops.stats().mean(Views.iterable(cropped)).getRealDouble();
        } else {
            Cursor<BitType> maskCursor = mask.localizingCursor();
            RandomAccess<T> dataRA = cropped.randomAccess();
            RealSum sum = new RealSum();
            int size = 0;
            maskCursor.reset();
            while (maskCursor.hasNext()) {
                maskCursor.fwd();
                if (maskCursor.get().get()) {
                    dataRA.setPosition(maskCursor);
                    sum.add(dataRA.get().getRealDouble());
                    size++;
                }
            }
            mean = sum.getSum() / size;
        }
        indices[z] = z;
        means[z] = mean;
    }
    return new ValuePair<double[], double[]>(indices, means);
}

Example 3 with IterableInterval

use of net.imglib2.IterableInterval in project vcell by virtualcell.

the class LargestRegionSlice method run.

@Override
public void run() {
    int maxArea = 0;
    int zOfMaxArea = 0;
    for (int z = 0; z < data.dimension(2); z++) {
        FinalInterval interval = Intervals.createMinMax(0, 0, z, data.dimension(0) - 1, data.dimension(1) - 1, z);
        RandomAccessibleInterval<BitType> croppedRAI = ops.transform().crop(data, interval);
        IterableInterval<BitType> croppedII = Views.iterable(croppedRAI);
        Cursor<BitType> cursor = croppedII.cursor();
        int area = 0;
        while (cursor.hasNext()) {
            if (cursor.next().get()) {
                area++;
            }
        }
        if (area > maxArea) {
            maxArea = area;
            zOfMaxArea = z;
        }
    }
    FinalInterval interval = Intervals.createMinMax(0, 0, zOfMaxArea, data.dimension(0) - 1, data.dimension(1) - 1, zOfMaxArea);
    output = ops.transform().crop(data, interval);
}