use of net.imglib2.Point in project vcell by virtualcell.
the class DeconstructGeometryCommand method run.
@Override
public void run() {
// Crop to get a z-stack over time (remove channel dimension)
long maxX = fluorData.max(fluorData.dimensionIndex(Axes.X));
long maxY = fluorData.max(fluorData.dimensionIndex(Axes.Y));
long maxZ = fluorData.max(fluorData.dimensionIndex(Axes.Z));
long maxTime = fluorData.max(fluorData.dimensionIndex(Axes.TIME));
Img fluorImg = fluorData.getImgPlus().getImg();
FinalInterval intervals = Intervals.createMinMax(0, 0, 0, 0, 0, maxX, maxY, maxZ, 0, maxTime);
RandomAccessibleInterval fluorImgCropped = ops.transform().crop(fluorImg, intervals, true);
// Calculate scale factors
double[] scaleFactors = { 1, 1, 1, 1 };
for (int i = 0; i < geomData.numDimensions(); i++) {
scaleFactors[i] = geomData.dimension(i) / (double) fluorImgCropped.dimension(i);
}
// Scale the fluorescence dataset to match the geometry
NLinearInterpolatorFactory interpolatorFactory = new NLinearInterpolatorFactory();
RandomAccessibleInterval fluorScaled = ops.transform().scale(fluorImgCropped, scaleFactors, interpolatorFactory);
// Crop out the first slice of each z-stack in time series
intervals = Intervals.createMinMax(0, 0, 0, 0, fluorScaled.dimension(0) - 1, fluorScaled.dimension(1) - 1, 0, fluorScaled.dimension(3) - 1);
IntervalView fluorXYT = (IntervalView) ops.transform().crop(fluorScaled, intervals, true);
// Create a blank image of the same X-Y-Time dimensions
long[] dimensions = { fluorXYT.dimension(0), fluorXYT.dimension(1), fluorXYT.dimension(2) };
Img<DoubleType> result = ops.create().img(dimensions);
// Calculate constant d in TIRF exponential decay function
theta = theta * 2 * Math.PI / 360;
double n1 = 1.52;
double n2 = 1.38;
double d = lambda * Math.pow((Math.pow(n1, 2) * Math.pow(Math.sin(theta), 2) - Math.pow(n2, 2)), -0.5) / (4 * Math.PI);
// Iterate through each time point, using 3D geometry to generate 2D intensities
Cursor<DoubleType> cursor = fluorXYT.localizingCursor();
RandomAccess fluorRA = fluorScaled.randomAccess();
RandomAccess<RealType<?>> geomRA = geomData.randomAccess();
RandomAccess<DoubleType> resultRA = result.randomAccess();
maxZ = geomData.dimension(2) - 1;
while (cursor.hasNext()) {
cursor.fwd();
int[] positionXYZ = { cursor.getIntPosition(0), cursor.getIntPosition(1), (int) maxZ - 1 };
int[] positionXYZT = { cursor.getIntPosition(0), cursor.getIntPosition(1), (int) maxZ - 1, cursor.getIntPosition(2) };
resultRA.setPosition(cursor);
geomRA.setPosition(positionXYZ);
double sum = 0.0;
while (positionXYZ[2] >= 0 && geomRA.get().getRealDouble() != 0.0) {
fluorRA.setPosition(positionXYZT);
geomRA.setPosition(positionXYZ);
sum += geomRA.get().getRealDouble() * Math.exp(-zSpacing * positionXYZ[2] / d);
positionXYZ[2]--;
}
resultRA.get().set(sum);
}
System.out.println("done");
displayService.createDisplay(result);
}
use of net.imglib2.Point in project java-docs-samples by GoogleCloudPlatform.
the class TimeSeriesSummary method getMostRecentPoint.
Point getMostRecentPoint(TimeSeries timeSeries) {
Point max = Collections.max(timeSeries.getPointsList(), Comparator.comparingLong(p -> p.getInterval().getEndTime().getSeconds()));
mostRecentRunTime = max.getInterval().getEndTime();
return max;
}
use of net.imglib2.Point in project imagej-ops by imagej.
the class PolygonFeatureTests method labelRegionToPolygonConverter.
@Test
public void labelRegionToPolygonConverter() {
// ground truth computed with matlab
final LabelRegionToPolygonConverter c = new LabelRegionToPolygonConverter();
c.setContext(ops.context());
final Polygon2D test = c.convert(ROI, Polygon2D.class);
final List<? extends RealLocalizable> expected = GeomUtils.vertices(contour);
final List<? extends RealLocalizable> received = GeomUtils.vertices(test);
assertEquals("Number of polygon points differs.", expected.size(), received.size());
for (int i = 0; i < contour.numVertices(); i++) {
assertEquals("Polygon point " + i + " differs in x-coordinate.", expected.get(i).getDoublePosition(0), received.get(i).getDoublePosition(0), EPSILON);
assertEquals("Polygon point " + i + " differs in y-coordinate.", expected.get(i).getDoublePosition(1), received.get(i).getDoublePosition(1), EPSILON);
}
}
use of net.imglib2.Point in project imagej-ops by imagej.
the class PolygonFeatureTests method smallesEnclosingRectangle.
@Test
public void smallesEnclosingRectangle() {
// ground truth verified with matlab
final List<? extends RealLocalizable> received = GeomUtils.vertices(((Polygon2D) ops.run(DefaultSmallestEnclosingRectangle.class, contour)));
final RealPoint[] expected = new RealPoint[] { new RealPoint(37.229184188393, -0.006307821699), new RealPoint(-14.757779646762, 27.800672834315), new RealPoint(31.725820016821, 114.704793944491), new RealPoint(83.712783851976, 86.897813288478) };
assertEquals("Number of polygon points differs.", expected.length, received.size());
for (int i = 0; i < expected.length; i++) {
assertEquals("Polygon point " + i + " differs in x-coordinate.", expected[i].getDoublePosition(0), received.get(i).getDoublePosition(0), EPSILON);
assertEquals("Polygon point " + i + " differs in y-coordinate.", expected[i].getDoublePosition(1), received.get(i).getDoublePosition(1), EPSILON);
}
}
use of net.imglib2.Point in project imagej-ops by imagej.
the class PolygonFeatureTests method convexHull2D.
@Test
public void convexHull2D() {
// ground truth computed with matlab
final Polygon2D test = (Polygon2D) ops.run(DefaultConvexHull2D.class, contour);
final List<? extends RealLocalizable> received = GeomUtils.vertices(test);
final RealPoint[] expected = new RealPoint[] { new RealPoint(1, 30), new RealPoint(2, 29), new RealPoint(26, 6), new RealPoint(31, 6), new RealPoint(42, 9), new RealPoint(49, 22), new RealPoint(72, 65), new RealPoint(78, 77), new RealPoint(48, 106), new RealPoint(42, 109), new RealPoint(34, 109), new RealPoint(28, 106), new RealPoint(26, 104), new RealPoint(23, 98) };
assertEquals("Number of polygon points differs.", expected.length, received.size());
for (int i = 0; i < expected.length; i++) {
assertEquals("Polygon point " + i + " differs in x-coordinate.", expected[i].getDoublePosition(0), received.get(i).getDoublePosition(0), EPSILON);
assertEquals("Polygon point " + i + " differs in y-coordinate.", expected[i].getDoublePosition(1), received.get(i).getDoublePosition(1), EPSILON);
}
}
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