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Example 1 with Intervals

use of net.imglib2.util.Intervals in project vcell by virtualcell.

the class DeconstructGeometryCommand method run.

@Override
public void run() {
    // Crop to get a z-stack over time (remove channel dimension)
    long maxX = fluorData.max(fluorData.dimensionIndex(Axes.X));
    long maxY = fluorData.max(fluorData.dimensionIndex(Axes.Y));
    long maxZ = fluorData.max(fluorData.dimensionIndex(Axes.Z));
    long maxTime = fluorData.max(fluorData.dimensionIndex(Axes.TIME));
    Img fluorImg = fluorData.getImgPlus().getImg();
    FinalInterval intervals = Intervals.createMinMax(0, 0, 0, 0, 0, maxX, maxY, maxZ, 0, maxTime);
    RandomAccessibleInterval fluorImgCropped = ops.transform().crop(fluorImg, intervals, true);
    // Calculate scale factors
    double[] scaleFactors = { 1, 1, 1, 1 };
    for (int i = 0; i < geomData.numDimensions(); i++) {
        scaleFactors[i] = geomData.dimension(i) / (double) fluorImgCropped.dimension(i);
    }
    // Scale the fluorescence dataset to match the geometry
    NLinearInterpolatorFactory interpolatorFactory = new NLinearInterpolatorFactory();
    RandomAccessibleInterval fluorScaled = ops.transform().scale(fluorImgCropped, scaleFactors, interpolatorFactory);
    // Crop out the first slice of each z-stack in time series
    intervals = Intervals.createMinMax(0, 0, 0, 0, fluorScaled.dimension(0) - 1, fluorScaled.dimension(1) - 1, 0, fluorScaled.dimension(3) - 1);
    IntervalView fluorXYT = (IntervalView) ops.transform().crop(fluorScaled, intervals, true);
    // Create a blank image of the same X-Y-Time dimensions
    long[] dimensions = { fluorXYT.dimension(0), fluorXYT.dimension(1), fluorXYT.dimension(2) };
    Img<DoubleType> result = ops.create().img(dimensions);
    // Calculate constant d in TIRF exponential decay function
    theta = theta * 2 * Math.PI / 360;
    double n1 = 1.52;
    double n2 = 1.38;
    double d = lambda * Math.pow((Math.pow(n1, 2) * Math.pow(Math.sin(theta), 2) - Math.pow(n2, 2)), -0.5) / (4 * Math.PI);
    // Iterate through each time point, using 3D geometry to generate 2D intensities
    Cursor<DoubleType> cursor = fluorXYT.localizingCursor();
    RandomAccess fluorRA = fluorScaled.randomAccess();
    RandomAccess<RealType<?>> geomRA = geomData.randomAccess();
    RandomAccess<DoubleType> resultRA = result.randomAccess();
    maxZ = geomData.dimension(2) - 1;
    while (cursor.hasNext()) {
        cursor.fwd();
        int[] positionXYZ = { cursor.getIntPosition(0), cursor.getIntPosition(1), (int) maxZ - 1 };
        int[] positionXYZT = { cursor.getIntPosition(0), cursor.getIntPosition(1), (int) maxZ - 1, cursor.getIntPosition(2) };
        resultRA.setPosition(cursor);
        geomRA.setPosition(positionXYZ);
        double sum = 0.0;
        while (positionXYZ[2] >= 0 && geomRA.get().getRealDouble() != 0.0) {
            fluorRA.setPosition(positionXYZT);
            geomRA.setPosition(positionXYZ);
            sum += geomRA.get().getRealDouble() * Math.exp(-zSpacing * positionXYZ[2] / d);
            positionXYZ[2]--;
        }
        resultRA.get().set(sum);
    }
    System.out.println("done");
    displayService.createDisplay(result);
}
Also used : Img(net.imglib2.img.Img) NLinearInterpolatorFactory(net.imglib2.interpolation.randomaccess.NLinearInterpolatorFactory) RandomAccess(net.imglib2.RandomAccess) RealType(net.imglib2.type.numeric.RealType) IntervalView(net.imglib2.view.IntervalView) RandomAccessibleInterval(net.imglib2.RandomAccessibleInterval) DoubleType(net.imglib2.type.numeric.real.DoubleType) FinalInterval(net.imglib2.FinalInterval)

Example 2 with Intervals

use of net.imglib2.util.Intervals in project imagej-ops by imagej.

the class ConcatenateViewTest method concatenateWithAccessModeTest.

@Test
public void concatenateWithAccessModeTest() {
    final List<RandomAccessibleInterval<ByteType>> intervals = createIntervals(img, divider, axis);
    for (final StackAccessMode mode : StackAccessMode.values()) {
        final RandomAccessibleInterval<ByteType> cat1 = Views.concatenate(axis, mode, intervals);
        final RandomAccessibleInterval<ByteType> cat2 = ops.transform().concatenateView(intervals, axis, mode);
        testEqual(cat1, cat2);
    }
}
Also used : StackAccessMode(net.imglib2.view.StackView.StackAccessMode) RandomAccessibleInterval(net.imglib2.RandomAccessibleInterval) ByteType(net.imglib2.type.numeric.integer.ByteType) AbstractOpTest(net.imagej.ops.AbstractOpTest) Test(org.junit.Test)

Aggregations

RandomAccessibleInterval (net.imglib2.RandomAccessibleInterval)2 AbstractOpTest (net.imagej.ops.AbstractOpTest)1 FinalInterval (net.imglib2.FinalInterval)1 RandomAccess (net.imglib2.RandomAccess)1 Img (net.imglib2.img.Img)1 NLinearInterpolatorFactory (net.imglib2.interpolation.randomaccess.NLinearInterpolatorFactory)1 RealType (net.imglib2.type.numeric.RealType)1 ByteType (net.imglib2.type.numeric.integer.ByteType)1 DoubleType (net.imglib2.type.numeric.real.DoubleType)1 IntervalView (net.imglib2.view.IntervalView)1 StackAccessMode (net.imglib2.view.StackView.StackAccessMode)1 Test (org.junit.Test)1