use of org.bytedeco.opencv.opencv_core.Mat in project qupath by qupath.
the class TestOpenCVTools method testPercentiles.
@Test
public void testPercentiles() {
int[] minValues = { -2, 0, 1 };
int[] maxValues = { 1, 10, 101 };
opencv_core.setRNGSeed(100);
for (int min : minValues) {
for (int max : maxValues) {
var values = IntStream.range(min, max + 1).asDoubleStream().toArray();
var stats = new DescriptiveStatistics(values);
var mat = new Mat(values);
opencv_core.randShuffle(mat);
assertEquals(stats.getPercentile(50), OpenCVTools.median(mat));
assertEquals((min + max) / 2.0, OpenCVTools.median(mat));
assertEquals(max, OpenCVTools.maximum(mat));
assertEquals(min, OpenCVTools.minimum(mat));
assertArrayEquals(new double[] { min, stats.getPercentile(50), max }, OpenCVTools.percentiles(mat, 1e-9, 50, 100));
double[] newValues = new double[values.length + 30];
Arrays.fill(newValues, Double.NaN);
System.arraycopy(values, 0, newValues, 0, values.length);
mat.close();
mat = new Mat(newValues);
opencv_core.randShuffle(mat);
assertEquals(stats.getPercentile(50), OpenCVTools.median(mat));
assertEquals((min + max) / 2.0, OpenCVTools.median(mat));
assertEquals(max, OpenCVTools.maximum(mat));
assertEquals(min, OpenCVTools.minimum(mat));
assertArrayEquals(new double[] { min, stats.getPercentile(50), max }, OpenCVTools.percentiles(mat, 1e-9, 50, 100));
mat.close();
}
}
}
use of org.bytedeco.opencv.opencv_core.Mat in project qupath by qupath.
the class PixelClassifierTraining method updateTrainingData.
private synchronized ClassifierTrainingData updateTrainingData(Map<PathClass, Integer> labelMap, Collection<ImageData<BufferedImage>> imageDataCollection) throws IOException {
if (imageDataCollection.isEmpty()) {
resetTrainingData();
return null;
}
Map<PathClass, Integer> labels = new LinkedHashMap<>();
boolean hasLockedAnnotations = false;
if (labelMap == null) {
Set<PathClass> pathClasses = new TreeSet<>((p1, p2) -> p1.toString().compareTo(p2.toString()));
for (var imageData : imageDataCollection) {
// Get labels for all annotations
Collection<PathObject> annotations = imageData.getHierarchy().getAnnotationObjects();
for (var annotation : annotations) {
if (isTrainableAnnotation(annotation, true)) {
var pathClass = annotation.getPathClass();
pathClasses.add(pathClass);
// We only use boundary classes for areas
if (annotation.getROI().isArea()) {
var boundaryClass = boundaryStrategy.getBoundaryClass(pathClass);
if (boundaryClass != null)
pathClasses.add(boundaryClass);
}
} else if (isTrainableAnnotation(annotation, false))
hasLockedAnnotations = true;
}
}
int lab = 0;
for (PathClass pathClass : pathClasses) {
Integer temp = Integer.valueOf(lab);
labels.put(pathClass, temp);
lab++;
}
} else {
labels.putAll(labelMap);
}
List<Mat> allFeatures = new ArrayList<>();
List<Mat> allTargets = new ArrayList<>();
for (var imageData : imageDataCollection) {
// Get features & targets for all the tiles that we need
var featureServer = getFeatureServer(imageData);
if (featureServer != null) {
var tiles = featureServer.getTileRequestManager().getAllTileRequests();
for (var tile : tiles) {
var tileFeatures = getTileFeatures(tile.getRegionRequest(), featureServer, boundaryStrategy, labels);
if (tileFeatures != null) {
allFeatures.add(tileFeatures.getFeatures());
allTargets.add(tileFeatures.getTargets());
}
}
} else {
logger.warn("Unable to generate features for {}", imageData);
}
}
// We need at least two classes for anything very meaningful to happen
int nTargets = labels.size();
if (nTargets <= 1) {
logger.warn("Unlocked annotations for at least two classes are required to train a classifier!");
if (hasLockedAnnotations)
logger.warn("Image contains annotations that *could* be used for training, except they are currently locked. Please unlock them if they should be used.");
resetTrainingData();
return null;
}
if (matTraining == null)
matTraining = new Mat();
if (matTargets == null)
matTargets = new Mat();
opencv_core.vconcat(new MatVector(allFeatures.toArray(Mat[]::new)), matTraining);
opencv_core.vconcat(new MatVector(allTargets.toArray(Mat[]::new)), matTargets);
logger.debug("Training data: {} x {}, Target data: {} x {}", matTraining.rows(), matTraining.cols(), matTargets.rows(), matTargets.cols());
if (matTraining.rows() == 0) {
logger.warn("No training data found - if you have training annotations, check the features are compatible with the current image.");
return null;
}
return new ClassifierTrainingData(labels, matTraining, matTargets);
}
use of org.bytedeco.opencv.opencv_core.Mat in project qupath by qupath.
the class WandToolCV method createShape.
@Override
protected Geometry createShape(MouseEvent e, double x, double y, boolean useTiles, Geometry addToShape) {
GeometryFactory factory = getGeometryFactory();
if (addToShape != null && pLast != null && pLast.distanceSq(x, y) < 2)
return null;
long startTime = System.currentTimeMillis();
QuPathViewer viewer = getViewer();
if (viewer == null)
return null;
double downsample = Math.max(1, Math.round(viewer.getDownsampleFactor() * 4)) / 4.0;
var regionStore = viewer.getImageRegionStore();
// Paint the image as it is currently being viewed
var type = wandType.get();
boolean doGray = type == WandType.GRAY;
BufferedImage imgTemp = doGray ? imgGray : imgBGR;
int nChannels = doGray ? 1 : 3;
Graphics2D g2d = imgTemp.createGraphics();
g2d.setColor(Color.BLACK);
g2d.setClip(0, 0, w, w);
g2d.fillRect(0, 0, w, w);
double xStart = Math.round(x - w * downsample * 0.5);
double yStart = Math.round(y - w * downsample * 0.5);
bounds.setFrame(xStart, yStart, w * downsample, w * downsample);
g2d.scale(1.0 / downsample, 1.0 / downsample);
g2d.translate(-xStart, -yStart);
regionStore.paintRegion(viewer.getServer(), g2d, bounds, viewer.getZPosition(), viewer.getTPosition(), downsample, null, null, viewer.getImageDisplay());
// regionStore.paintRegionCompletely(viewer.getServer(), g2d, bounds, viewer.getZPosition(), viewer.getTPosition(), viewer.getDownsampleFactor(), null, viewer.getImageDisplay(), 250);
// Optionally include the overlay information when using the wand
float opacity = viewer.getOverlayOptions().getOpacity();
if (opacity > 0 && getWandUseOverlays()) {
ImageRegion region = ImageRegion.createInstance((int) bounds.getX() - 1, (int) bounds.getY() - 1, (int) bounds.getWidth() + 2, (int) bounds.getHeight() + 2, viewer.getZPosition(), viewer.getTPosition());
if (opacity < 1)
g2d.setComposite(AlphaComposite.getInstance(AlphaComposite.SRC_OVER, opacity));
for (PathOverlay overlay : viewer.getOverlayLayers().toArray(PathOverlay[]::new)) {
if (!(overlay instanceof HierarchyOverlay))
overlay.paintOverlay(g2d, region, downsample, viewer.getImageData(), true);
}
}
// Ensure we have Mats & the correct channel number
if (mat != null && (mat.channels() != nChannels || mat.depth() != opencv_core.CV_8U)) {
mat.close();
mat = null;
}
if (mat == null || mat.isNull() || mat.empty())
mat = new Mat(w, w, CV_8UC(nChannels));
// if (matMask == null)
// matMask = new Mat(w+2, w+2, CV_8U);
// if (matSelected == null)
// matSelected = new Mat(w+2, w+2, CV_8U);
// Put pixels into an OpenCV image
byte[] buffer = ((DataBufferByte) imgTemp.getRaster().getDataBuffer()).getData();
ByteBuffer matBuffer = mat.createBuffer();
matBuffer.put(buffer);
// mat.put(0, 0, buffer);
// opencv_imgproc.cvtColor(mat, mat, opencv_imgproc.COLOR_BGR2Lab);
// blurSigma = 4;
boolean doSimpleSelection = e.isShortcutDown() && !e.isShiftDown();
if (doSimpleSelection) {
matMask.put(Scalar.ZERO);
// opencv_imgproc.circle(matMask, seed, radius, Scalar.ONE);
opencv_imgproc.floodFill(mat, matMask, seed, Scalar.ONE, null, Scalar.ZERO, Scalar.ZERO, 4 | (2 << 8) | opencv_imgproc.FLOODFILL_MASK_ONLY | opencv_imgproc.FLOODFILL_FIXED_RANGE);
subtractPut(matMask, Scalar.ONE);
} else {
double blurSigma = Math.max(0.5, getWandSigmaPixels());
int size = (int) Math.ceil(blurSigma * 2) * 2 + 1;
blurSize.width(size);
blurSize.height(size);
// Smooth a little
opencv_imgproc.GaussianBlur(mat, mat, blurSize, blurSigma);
// Choose mat to threshold (may be adjusted)
Mat matThreshold = mat;
// Apply color transform if required
if (type == WandType.LAB_DISTANCE) {
mat.convertTo(matFloat, opencv_core.CV_32F, 1.0 / 255.0, 0.0);
opencv_imgproc.cvtColor(matFloat, matFloat, opencv_imgproc.COLOR_BGR2Lab);
double max = 0;
double mean = 0;
try (FloatIndexer idx = matFloat.createIndexer()) {
int k = w / 2;
double v1 = idx.get(k, k, 0);
double v2 = idx.get(k, k, 1);
double v3 = idx.get(k, k, 2);
double meanScale = 1.0 / (w * w);
for (int row = 0; row < w; row++) {
for (int col = 0; col < w; col++) {
double L = idx.get(row, col, 0) - v1;
double A = idx.get(row, col, 1) - v2;
double B = idx.get(row, col, 2) - v3;
double dist = Math.sqrt(L * L + A * A + B * B);
if (dist > max)
max = dist;
mean += dist * meanScale;
idx.put(row, col, 0, (float) dist);
}
}
}
if (matThreshold == null)
matThreshold = new Mat();
opencv_core.extractChannel(matFloat, matThreshold, 0);
// There are various ways we might choose a threshold now...
// Here, we use a multiple of the mean. Since values are 'distances'
// they are all >= 0
matThreshold.convertTo(matThreshold, opencv_core.CV_8U, 255.0 / max, 0);
threshold.put(mean * getWandSensitivity());
// // OpenCVTools.matToImagePlus(matThreshold, "Before").show();
// // Apply local Otsu threshold
// opencv_imgproc.threshold(matThreshold, matThreshold,
// 0,
// 255, opencv_imgproc.THRESH_BINARY + opencv_imgproc.THRESH_OTSU);
// threshold.put(Scalar.ZERO);
nChannels = 1;
} else {
// Base threshold on local standard deviation
meanStdDev(matThreshold, mean, stddev);
DoubleBuffer stddevBuffer = stddev.createBuffer();
double[] stddev2 = new double[nChannels];
stddevBuffer.get(stddev2);
double scale = 1.0 / getWandSensitivity();
if (scale < 0)
scale = 0.01;
for (int i = 0; i < stddev2.length; i++) stddev2[i] = stddev2[i] * scale;
threshold.put(stddev2);
}
// Limit maximum radius by pen
int radius = (int) Math.round(w / 2 * QuPathPenManager.getPenManager().getPressure());
if (radius == 0)
return null;
matMask.put(Scalar.ZERO);
opencv_imgproc.circle(matMask, seed, radius, Scalar.ONE);
opencv_imgproc.floodFill(matThreshold, matMask, seed, Scalar.ONE, null, threshold, threshold, 4 | (2 << 8) | opencv_imgproc.FLOODFILL_MASK_ONLY | opencv_imgproc.FLOODFILL_FIXED_RANGE);
subtractPut(matMask, Scalar.ONE);
if (strel == null)
strel = opencv_imgproc.getStructuringElement(opencv_imgproc.MORPH_ELLIPSE, new Size(5, 5));
opencv_imgproc.morphologyEx(matMask, matMask, opencv_imgproc.MORPH_CLOSE, strel);
}
MatVector contours = new MatVector();
if (contourHierarchy == null)
contourHierarchy = new Mat();
opencv_imgproc.findContours(matMask, contours, contourHierarchy, opencv_imgproc.RETR_EXTERNAL, opencv_imgproc.CHAIN_APPROX_SIMPLE);
// logger.trace("Contours: " + contours.size());
List<Coordinate> coords = new ArrayList<>();
List<Geometry> geometries = new ArrayList<>();
for (Mat contour : contours.get()) {
// Discard single pixels / lines
if (contour.size().height() <= 2)
continue;
// Create a polygon geometry
try (IntIndexer idxrContours = contour.createIndexer()) {
for (long r = 0; r < idxrContours.size(0); r++) {
int px = idxrContours.get(r, 0L, 0L);
int py = idxrContours.get(r, 0L, 1L);
// * downsample + x;
double xx = (px - w / 2 - 1);
// * downsample + y;
double yy = (py - w / 2 - 1);
coords.add(new Coordinate(xx, yy));
}
}
if (coords.size() > 1) {
// Ensure closed
if (!coords.get(coords.size() - 1).equals(coords.get(0)))
coords.add(coords.get(0));
// Exclude single pixels
var polygon = factory.createPolygon(coords.toArray(Coordinate[]::new));
if (coords.size() > 5 || polygon.getArea() > 1)
geometries.add(polygon);
}
}
contours.close();
if (geometries.isEmpty())
return null;
// Handle the fact that OpenCV contours are defined using the 'pixel center' by dilating the boundary
var geometry = geometries.size() == 1 ? geometries.get(0) : GeometryCombiner.combine(geometries);
geometry = geometry.buffer(0.5);
// Transform to map to integer pixel locations in the full-resolution image
var transform = new AffineTransformation().scale(downsample, downsample).translate(x, y);
geometry = transform.transform(geometry);
geometry = GeometryTools.roundCoordinates(geometry);
geometry = GeometryTools.constrainToBounds(geometry, 0, 0, viewer.getServerWidth(), viewer.getServerHeight());
if (geometry.getArea() <= 1)
return null;
long endTime = System.currentTimeMillis();
logger.trace(getClass().getSimpleName() + " time: " + (endTime - startTime));
if (pLast == null)
pLast = new Point2D.Double(x, y);
else
pLast.setLocation(x, y);
return geometry;
}
use of org.bytedeco.opencv.opencv_core.Mat in project qupath by qupath.
the class DetectCytokeratinCV method updateArea.
private static void updateArea(final MatVector contours, final Mat hierarchy, final Area area, int row, int depth) {
IntIndexer indexer = hierarchy.createIndexer();
while (row >= 0) {
int[] data = new int[4];
// TODO: Check indexing after switch to JavaCPP!!!
indexer.get(0, row, data);
// hierarchy.get(0, row, data);
Mat contour = contours.get(row);
// Don't include isolated pixels - otherwise add or remove, as required
if (contour.rows() > 2) {
Path2D path = getContour(contour);
if (depth % 2 == 0)
area.add(new Area(path));
else
area.subtract(new Area(path));
}
// Deal with any sub-contours
if (data[2] >= 0)
updateArea(contours, hierarchy, area, data[2], depth + 1);
// Move to next contour in this hierarchy level
row = data[0];
}
}
use of org.bytedeco.opencv.opencv_core.Mat in project qupath by qupath.
the class DetectCytokeratinCV method getArea.
/**
* Get an Area object corresponding to contours in a binary image from OpenCV.
* @param mat
* @return
*/
private static Area getArea(final Mat mat) {
if (mat.empty())
return null;
// Identify all contours
MatVector contours = new MatVector();
Mat hierarchy = new Mat();
opencv_imgproc.findContours(mat, contours, hierarchy, opencv_imgproc.RETR_TREE, opencv_imgproc.CHAIN_APPROX_SIMPLE);
if (contours.empty()) {
hierarchy.close();
return null;
}
Area area = new Area();
updateArea(contours, hierarchy, area, 0, 0);
hierarchy.close();
return area;
}
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