use of org.sbolstandard.core2.SBOLDocument in project libSBOLj by SynBioDex.
the class MapsToTest method setUp.
@Before
public void setUp() throws Exception {
String prURI = "http://partsregistry.org";
doc = new SBOLDocument();
doc.setDefaultURIprefix(prURI);
doc.setTypesInURIs(false);
doc.setComplete(true);
/*create CD's for main CD and sub-components*/
gRNA_b_gene = doc.createComponentDefinition("gRNA_b_gene", "", ComponentDefinition.DNA);
doc.createComponentDefinition("promoter_CD", "", ComponentDefinition.DNA);
gene_CD = doc.createComponentDefinition("gene_CD", "", ComponentDefinition.DNA);
doc.createComponentDefinition("terminator_CD", "", ComponentDefinition.DNA);
gRNA_b_gene.createComponent("promoter", AccessType.PUBLIC, "promoter_CD");
gene = gRNA_b_gene.createComponent("gene", AccessType.PUBLIC, "gene_CD");
gRNA_b_gene.createComponent("terminator", AccessType.PUBLIC, "terminator_CD");
doc.createComponentDefinition("target_gene_CD", "", ComponentDefinition.DNA);
target_gene = gRNA_b_gene.createComponent("target_gene", AccessType.PUBLIC, "target_gene_CD");
doc.createComponentDefinition("target_protein", ComponentDefinition.DNA);
protein = gene_CD.createComponent("protein", AccessType.PUBLIC, "target_protein");
}
use of org.sbolstandard.core2.SBOLDocument in project libSBOLj by SynBioDex.
the class Provenance_StrainDerivation method main.
public static void main(String[] args) throws Exception {
NamespaceBinding myAppNs = NamespaceBinding("http://myapp.com/", "myapp");
SBOLDocument document = new SBOLDocument();
document.addNamespace(URI.create(myAppNs.getNamespaceURI()), myAppNs.getPrefix());
document.setDefaultURIprefix(myAppNs.getNamespaceURI());
ComponentDefinition b168 = getCds(document, "bsubtilis168", "Bacillus subtilis 168");
ComponentDefinition b3610 = getCds(document, "bsubtilisncimb3610", "Bacillus subtilis NCIMB 3610");
b168.addWasDerivedFrom(b3610.getIdentity());
// Create the agent definition to represent X-ray
Agent agent = document.createAgent("x_ray");
agent.setName("X-ray");
// Create the generic top level entity for the X-ray mutagenesis activity
Activity activity = document.createActivity("xraymutagenesis");
activity.setName("X-ray mutagenesis");
// Create the qualifiedUsage annotation to describe the use of the parent strain
activity.createUsage("usage", b3610.getIdentity()).addRole(URI.create("http://sbols.org/v2#source"));
// Create the qualifiedAssociation annotation to describe the use of the agent used in the activity
activity.createAssociation("association", agent.getIdentity()).addRole(myAppNs.namespacedUri("mutagen"));
b168.addWasGeneratedBy(activity.getIdentity());
SBOLWriter.write(document, System.out);
}
use of org.sbolstandard.core2.SBOLDocument in project libSBOLj by SynBioDex.
the class SequenceAnnotationTest method setUp.
@Before
public void setUp() throws Exception {
String prURI = "http://partsregistry.org";
doc = new SBOLDocument();
doc.setDefaultURIprefix(prURI);
doc.setTypesInURIs(false);
doc.setComplete(true);
/*create CD's for main CD and sub-components*/
gRNA_b_gene = doc.createComponentDefinition("gRNA_b_gene", "", ComponentDefinition.DNA);
promoter_CD = doc.createComponentDefinition("promoter_CD", "", ComponentDefinition.DNA);
doc.createComponentDefinition("gene_CD", "", ComponentDefinition.DNA);
doc.createComponentDefinition("terminator_CD", "", ComponentDefinition.DNA);
gRNA_b_gene.createComponent("promoter", AccessType.PUBLIC, "promoter_CD");
gRNA_b_gene.createComponent("gene", AccessType.PUBLIC, "promoter_CD");
gRNA_b_gene.createComponent("terminator", AccessType.PUBLIC, "promoter_CD");
/*create SequenceAnnotations*/
promoter_SA = gRNA_b_gene.createSequenceAnnotation("promoter_SA", "cutAt1");
promoter_SA.setComponent("promoter");
gene_SA = gRNA_b_gene.createSequenceAnnotation("gene_SA", "cutAt50");
terminator_SA = gRNA_b_gene.createSequenceAnnotation("terminator_SA", "cutAt100");
}
use of org.sbolstandard.core2.SBOLDocument in project libSBOLj by SynBioDex.
the class CollectionOutput method main.
/**
* @param args
* @throws SBOLValidationException see SBOL validation rule violation at {@link Collection#addMember(URI)}
* @throws SBOLConversionException
*/
public static void main(String[] args) throws SBOLValidationException, SBOLConversionException {
SBOLDocument document = new SBOLDocument();
document.setDefaultURIprefix("http://parts.igem.org/Promoters/Catalog");
document.setTypesInURIs(false);
Collection col = document.createCollection("Anderson", "");
col.setName("Anderson promoters");
col.setDescription("The Anderson promoter collection");
col.addMember(URI.create("http://partsregistry.org/Part:BBa_J23119"));
col.addMember(URI.create("http://partsregistry.org/Part:BBa_J23118"));
SBOLWriter.write(document, (System.out));
}
use of org.sbolstandard.core2.SBOLDocument in project libSBOLj by SynBioDex.
the class GettingStartedExample method main.
public static void main(String[] args) throws IOException, SBOLValidationException, SBOLConversionException {
String prURI = "http://partsregistry.org/";
String prPrefix = "pr";
String myersLabURI = "http://www.async.ece.utah.edu/";
String myersLabPrefix = "myersLab";
SBOLDocument document = new SBOLDocument();
document.setDefaultURIprefix(prURI);
document.setTypesInURIs(true);
document.setComplete(true);
document.setCreateDefaults(true);
document.addNamespace(URI.create(prURI), prPrefix);
document.addNamespace(URI.create(myersLabURI), myersLabPrefix);
// Creating a Top-level SBOL Data Object
HashSet<URI> types = new HashSet<URI>(Arrays.asList(ComponentDefinition.DNA, URI.create("http://identifiers.org/chebi/CHEBI:4705")));
ComponentDefinition TetR_promoter = document.createComponentDefinition("BBa_R0040", types);
ComponentDefinition LacI_repressor = document.createComponentDefinition("BBa_C0012", types);
ComponentDefinition pIKELeftCassette = document.createComponentDefinition("pIKELeftCassette", types);
Sequence seq_187 = document.createSequence("partseq_187", "tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac", Sequence.IUPAC_DNA);
String element2 = "atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtc" + "tcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcggga" + "aaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactgg" + "cgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcg" + "caaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgat" + "ggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcg" + "tcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcc" + "tgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattat" + "tttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagc" + "aaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctgg" + "cataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgc" + "catgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgc" + "tggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgc" + "gttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatccc" + "gccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgc" + "tgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaa" + "agaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcatt" + "aatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaact" + "acgctttagtagcttaataa";
Sequence seq_153 = document.createSequence("partseq_153", element2, Sequence.IUPAC_DNA);
// Setting and editing optional fields
TetR_promoter.setName("p(tetR)");
LacI_repressor.setName("lacI");
TetR_promoter.setDescription("TetR repressible promoter");
LacI_repressor.setDescription("lacI repressor from E. coli (+LVA)");
if (TetR_promoter.isSetName()) {
TetR_promoter.unsetName();
}
TetR_promoter.setName("p(tetR)");
TetR_promoter.addRole(SequenceOntology.PROMOTER);
LacI_repressor.addRole(SequenceOntology.CDS);
URI TetR_promoter_role2 = URI.create("http://identifiers.org/so/SO:0000613");
TetR_promoter.addRole(TetR_promoter_role2);
if (TetR_promoter.containsRole(TetR_promoter_role2)) {
TetR_promoter.removeRole(TetR_promoter_role2);
}
TetR_promoter.clearRoles();
if (!TetR_promoter.getRoles().isEmpty()) {
System.out.println("TetR_promoter set is not empty");
}
TetR_promoter.setRoles(new HashSet<URI>(Arrays.asList(SequenceOntology.PROMOTER)));
// Creating and editing references
TetR_promoter.addSequence(seq_187);
LacI_repressor.addSequence(seq_153);
pIKELeftCassette.addSequence(seq_187);
pIKELeftCassette.clearSequences();
// Adding the sequence below causes an exception because it cannot be found
// pIKELeftCassette.addSequence(URI.create("http://partsregistry.org/seq/partseq_154"));
// Creating Annotations
TetR_promoter.createAnnotation(new QName(prURI, "experience", prPrefix), URI.create("http://parts.igem.org/Part:BBa_R0040"));
// Creating Generic TopLevel Object
GenericTopLevel datasheet = document.createGenericTopLevel("datasheet", "1.0", new QName(myersLabURI, "datasheet", myersLabPrefix));
datasheet.setName("Datasheet for Custom Parameters");
datasheet.createAnnotation(new QName(myersLabURI, "characterizationData", myersLabPrefix), URI.create(myersLabURI + "/measurement/Part:BBa_R0040"));
datasheet.createAnnotation(new QName(myersLabURI, "transcriptionRate", myersLabPrefix), "0.75");
TetR_promoter.createAnnotation(new QName(myersLabURI, "datasheet", myersLabPrefix), datasheet.getIdentity());
// Creating and editing Child Objects
// For pIKELeftCassette, create sequence constraint that says BBa_R0040 precedes BBa_C0012.
// Note that with CreateDefaults that components get created automatically.
// The position of the subject Component MUST precede that of the object Component.
pIKELeftCassette.createSequenceConstraint("pIKELeftCassette_sc", RestrictionType.PRECEDES, TetR_promoter.getDisplayId(), LacI_repressor.getDisplayId());
if (pIKELeftCassette.getComponent("BBa_R0040") == null) {
System.out.println("TetR_promoter component is missing");
}
if (pIKELeftCassette.getComponent("BBa_C0012") == null) {
System.out.println("LacI_repressor component is missing");
}
// Removing the subject component below causes an exception.
// pIKELeftCassette.removeComponent(pIKELeftCassette.getComponent("BBa_R0040"));
// Copying objects
ComponentDefinition TetR_promoter_copy = (ComponentDefinition) document.createCopy(TetR_promoter, "BBa_K137046");
Sequence seq = document.createSequence("seq_K137046", "gtgctcagtatctctatcactgatagggatgtcaatctctatcactgatagggactctagtatat" + "aaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgaca" + "aacaacagataaaacgaaaggc", Sequence.IUPAC_DNA);
TetR_promoter_copy.addSequence(seq);
SBOLWriter.write(document, "GettingStartedExample.rdf");
writeThenRead(document);
}
Aggregations