Examples with LineROI qupath.lib.roi.LineROI used on opensource projects

Example 1 with LineROI

use of qupath.lib.roi.LineROI in project qupath by qupath.

the class IconFactory method createROIIcon.

/**
 * Create an icon depicting a ROI.
 * @param roi the region of interest
 * @param width the preferred icon width
 * @param height the preferred icon height
 * @param color the icon (line) color
 * @return a node that may be used as an icon resembling the shape of the ROI
 */
public static Node createROIIcon(ROI roi, int width, int height, Color color) {
    double scale = Math.min(width / roi.getBoundsWidth(), height / roi.getBoundsHeight());
    if (roi instanceof RectangleROI) {
        Rectangle rect = new Rectangle(0, 0, roi.getBoundsWidth() * scale, roi.getBoundsHeight() * scale);
        rect.setStroke(color);
        rect.setFill(null);
        return rect;
    } else if (roi instanceof EllipseROI) {
        double w = roi.getBoundsWidth() * scale;
        double h = roi.getBoundsHeight() * scale;
        Ellipse ellipse = new Ellipse(w / 2, height / 2, w / 2, h / 2);
        ellipse.setStroke(color);
        ellipse.setFill(null);
        return ellipse;
    } else if (roi instanceof LineROI) {
        LineROI l = (LineROI) roi;
        double xMin = Math.min(l.getX1(), l.getX2());
        double yMin = Math.min(l.getY1(), l.getY2());
        Line line = new Line((l.getX1() - xMin) * scale, (l.getY1() - yMin) * scale, (l.getX2() - xMin) * scale, (l.getY2() - yMin) * scale);
        line.setStroke(color);
        line.setFill(null);
        return line;
    } else if (roi.isPoint()) {
        // Just show generic points
        Node node = IconFactory.createNode(Math.min(width, height), Math.min(width, height), IconFactory.PathIcons.POINTS_TOOL);
        if (node instanceof Glyph) {
            var glyph = (Glyph) node;
            glyph.textFillProperty().unbind();
            glyph.setColor(color);
        }
        return node;
    } else {
        var path = pathCache.getOrDefault(roi, null);
        if (path == null) {
            var shape = roi.isArea() ? RoiTools.getArea(roi) : RoiTools.getShape(roi);
            if (shape != null) {
                var transform = new AffineTransform();
                transform.translate(-roi.getBoundsX(), -roi.getBoundsY());
                transform.scale(scale, scale);
                PathIterator iterator = shape.getPathIterator(transform, Math.max(0.5, 1.0 / scale));
                path = createShapeIcon(iterator, color);
                pathCache.put(roi, path);
            }
        } else {
            path = new Path(path.getElements());
            path.setStroke(color);
        }
        if (path != null)
            return path;
    }
    logger.warn("Unable to create icon for ROI: {}", roi);
    return null;
}

Example 2 with LineROI

use of qupath.lib.roi.LineROI in project qupath by qupath.

the class PathHierarchyPaintingHelper method paintObject.

/**
 * Paint an object (or, more precisely, its ROI), optionally along with the ROIs of any child objects.
 *
 * This is subject to the OverlayOptions, and therefore may not actually end up painting anything
 * (if the settings are such that objects of the class provided are not to be displayed)
 *
 * @param pathObject
 * @param paintChildren
 * @param g
 * @param boundsDisplayed
 * @param overlayOptions
 * @param selectionModel
 * @param downsample
 * @return true if anything was painted, false otherwise
 */
public static boolean paintObject(PathObject pathObject, boolean paintChildren, Graphics2D g, Rectangle boundsDisplayed, OverlayOptions overlayOptions, PathObjectSelectionModel selectionModel, double downsample) {
    if (pathObject == null)
        return false;
    // g.setRenderingHint(RenderingHints.KEY_ALPHA_INTERPOLATION, RenderingHints.VALUE_ALPHA_INTERPOLATION_SPEED);
    // g.setRenderingHint(RenderingHints.KEY_ALPHA_INTERPOLATION, RenderingHints.VALUE_ALPHA_INTERPOLATION_QUALITY);
    // Always paint the selected object
    // Note: this makes the assumption that child ROIs are completely contained within their parents;
    // this probably should be the case, but isn't guaranteed
    boolean isSelected = (selectionModel != null && selectionModel.isSelected(pathObject)) && (PathPrefs.useSelectedColorProperty().get() || !PathObjectTools.hasPointROI(pathObject));
    boolean isDetectedObject = pathObject.isDetection() || (pathObject.isTile() && pathObject.hasMeasurements());
    // Check if the PathClass isn't being shown
    PathClass pathClass = pathObject.getPathClass();
    if (!isSelected && overlayOptions != null && overlayOptions.isPathClassHidden(pathClass))
        return false;
    boolean painted = false;
    // See if we need to check the children
    ROI pathROI = pathObject.getROI();
    if (pathROI != null) {
        double roiBoundsX = pathROI.getBoundsX();
        double roiBoundsY = pathROI.getBoundsY();
        double roiBoundsWidth = pathROI.getBoundsWidth();
        double roiBoundsHeight = pathROI.getBoundsHeight();
        if (PathObjectTools.hasPointROI(pathObject) || boundsDisplayed == null || pathROI instanceof LineROI || boundsDisplayed.intersects(roiBoundsX, roiBoundsY, Math.max(roiBoundsWidth, 1), Math.max(roiBoundsHeight, 1))) {
            // Paint the ROI, if necessary
            if (isSelected || (overlayOptions.getShowDetections() && isDetectedObject) || (overlayOptions.getShowAnnotations() && pathObject.isAnnotation()) || (overlayOptions.getShowTMAGrid() && pathObject.isTMACore())) {
                boolean doFill = overlayOptions.getFillDetections() || pathObject instanceof ParallelTileObject;
                boolean doOutline = true;
                Color color = null;
                boolean useMapper = false;
                double fillOpacity = .75;
                if (isSelected && PathPrefs.useSelectedColorProperty().get() && PathPrefs.colorSelectedObjectProperty().getValue() != null)
                    color = ColorToolsAwt.getCachedColor(PathPrefs.colorSelectedObjectProperty().get());
                else {
                    MeasurementMapper mapper = overlayOptions.getMeasurementMapper();
                    useMapper = mapper != null && mapper.isValid() && pathObject.isDetection();
                    if (useMapper) {
                        if (pathObject.hasMeasurements()) {
                            Integer rgb = mapper.getColorForObject(pathObject);
                            // If the mapper returns null, the object shouldn't be painted
                            if (rgb == null)
                                return false;
                            // , mapper.getColorMapper().hasAlpha());
                            color = ColorToolsAwt.getCachedColor(rgb);
                        } else
                            color = null;
                        // System.out.println(color + " - " + pathObject.getMeasurementList().getMeasurementValue(mapper.));
                        fillOpacity = 1.0;
                        // Outlines are not so helpful with the measurement mapper
                        if (doFill)
                            doOutline = doOutline && !pathObject.isTile();
                    } else {
                        Integer rgb = ColorToolsFX.getDisplayedColorARGB(pathObject);
                        color = ColorToolsAwt.getCachedColor(rgb);
                    }
                // color = PathObjectHelpers.getDisplayedColor(pathObject);
                }
                // Check if we have only one or two pixels to draw - if so, we can be done quickly
                if (isDetectedObject && downsample > 4 && roiBoundsWidth / downsample < 3 && roiBoundsHeight / downsample < 3) {
                    int x = (int) roiBoundsX;
                    int y = (int) roiBoundsY;
                    // Prefer rounding up, lest we lose a lot of regions unnecessarily
                    int w = (int) (roiBoundsWidth + .9);
                    int h = (int) (roiBoundsHeight + .9);
                    if (w > 0 && h > 0) {
                        g.setColor(color);
                        // g.setColor(DisplayHelpers.getMoreTranslucentColor(color));
                        // g.setStroke(getCachedStroke(overlayOptions.strokeThinThicknessProperty().get()));
                        g.fillRect(x, y, w, h);
                    }
                    painted = true;
                } else {
                    Stroke stroke = null;
                    // Decide whether to fill or not
                    Color colorFill = doFill && (isDetectedObject || PathObjectTools.hasPointROI(pathObject)) ? color : null;
                    if (colorFill != null && fillOpacity != 1) {
                        if (pathObject instanceof ParallelTileObject)
                            colorFill = ColorToolsAwt.getMoreTranslucentColor(colorFill);
                        else if (pathObject instanceof PathCellObject && overlayOptions.getShowCellBoundaries() && overlayOptions.getShowCellNuclei()) {
                            // if (isSelected)
                            // colorFill = ColorToolsAwt.getTranslucentColor(colorFill);
                            // else
                            colorFill = ColorToolsAwt.getMoreTranslucentColor(colorFill);
                        } else if (pathObject.getParent() instanceof PathDetectionObject) {
                            colorFill = ColorToolsAwt.getTranslucentColor(colorFill);
                        } else if (pathObject instanceof PathTileObject && pathClass == null && color != null && color.getRGB() == PathPrefs.colorTileProperty().get()) {
                            // Don't fill in empty, unclassified tiles
                            // DisplayHelpers.getMoreTranslucentColor(colorFill);
                            colorFill = null;
                        }
                    }
                    // Color colorStroke = doOutline ? (colorFill == null ? color : (downsample > overlayOptions.strokeThinThicknessProperty().get() ? null : DisplayHelpers.darkenColor(color))) : null;
                    Color colorStroke = doOutline ? (colorFill == null ? color : ColorToolsAwt.darkenColor(color)) : null;
                    // For thick lines, antialiasing is very noticeable... less so for thin lines (of which there may be a huge number)
                    if (isDetectedObject) {
                        // Detections inside detections get half the line width
                        if (pathObject.getParent() instanceof PathDetectionObject)
                            stroke = getCachedStroke(PathPrefs.detectionStrokeThicknessProperty().get() / 2.0);
                        else
                            stroke = getCachedStroke(PathPrefs.detectionStrokeThicknessProperty().get());
                    } else {
                        double thicknessScale = downsample * (isSelected && !PathPrefs.useSelectedColorProperty().get() ? 1.6 : 1);
                        float thickness = (float) (PathPrefs.annotationStrokeThicknessProperty().get() * thicknessScale);
                        if (isSelected && pathObject.getParent() == null && PathPrefs.selectionModeProperty().get()) {
                            stroke = getCachedStrokeDashed(thickness);
                        } else {
                            stroke = getCachedStroke(thickness);
                        }
                    }
                    g.setStroke(stroke);
                    boolean paintSymbols = overlayOptions.getDetectionDisplayMode() == DetectionDisplayMode.CENTROIDS && pathObject.isDetection() && !pathObject.isTile();
                    if (paintSymbols) {
                        pathROI = PathObjectTools.getROI(pathObject, true);
                        double x = pathROI.getCentroidX();
                        double y = pathROI.getCentroidY();
                        double radius = PathPrefs.detectionStrokeThicknessProperty().get() * 2.0;
                        if (pathObject.getParent() instanceof PathDetectionObject)
                            radius /= 2.0;
                        Shape shape;
                        int nSubclasses = 0;
                        if (pathClass != null) {
                            nSubclasses = PathClassTools.splitNames(pathClass).size();
                        }
                        switch(nSubclasses) {
                            case 0:
                                var ellipse = localEllipse2D.get();
                                ellipse.setFrame(x - radius, y - radius, radius * 2, radius * 2);
                                shape = ellipse;
                                break;
                            case 1:
                                var rect = localRect2D.get();
                                rect.setFrame(x - radius, y - radius, radius * 2, radius * 2);
                                shape = rect;
                                break;
                            case 2:
                                var triangle = localPath2D.get();
                                double sqrt3 = Math.sqrt(3.0);
                                triangle.reset();
                                triangle.moveTo(x, y - radius * 2.0 / sqrt3);
                                triangle.lineTo(x - radius, y + radius / sqrt3);
                                triangle.lineTo(x + radius, y + radius / sqrt3);
                                triangle.closePath();
                                shape = triangle;
                                break;
                            case 3:
                                var plus = localPath2D.get();
                                plus.reset();
                                plus.moveTo(x, y - radius);
                                plus.lineTo(x, y + radius);
                                plus.moveTo(x - radius, y);
                                plus.lineTo(x + radius, y);
                                shape = plus;
                                break;
                            default:
                                var cross = localPath2D.get();
                                cross.reset();
                                radius /= Math.sqrt(2);
                                cross.moveTo(x - radius, y - radius);
                                cross.lineTo(x + radius, y + radius);
                                cross.moveTo(x + radius, y - radius);
                                cross.lineTo(x - radius, y + radius);
                                shape = cross;
                                break;
                        }
                        paintShape(shape, g, colorStroke, stroke, colorFill);
                    } else if (pathObject instanceof PathCellObject) {
                        PathCellObject cell = (PathCellObject) pathObject;
                        if (overlayOptions.getShowCellBoundaries())
                            paintROI(pathROI, g, colorStroke, stroke, colorFill, downsample);
                        if (overlayOptions.getShowCellNuclei())
                            paintROI(cell.getNucleusROI(), g, colorStroke, stroke, colorFill, downsample);
                        painted = true;
                    } else {
                        if ((overlayOptions.getFillAnnotations() && pathObject.isAnnotation() && pathObject.getPathClass() != PathClassFactory.getPathClass(StandardPathClasses.REGION) && (pathObject.getPathClass() != null || !pathObject.hasChildren())) || (pathObject.isTMACore() && overlayOptions.getShowTMACoreLabels()))
                            paintROI(pathROI, g, colorStroke, stroke, ColorToolsAwt.getMoreTranslucentColor(colorStroke), downsample);
                        else
                            paintROI(pathROI, g, colorStroke, stroke, colorFill, downsample);
                        painted = true;
                    }
                }
            }
        }
    }
    // Paint the children, if necessary
    if (paintChildren) {
        for (PathObject childObject : pathObject.getChildObjectsAsArray()) {
            // Only call the painting method if required
            ROI childROI = childObject.getROI();
            if ((childROI != null && boundsDisplayed.intersects(childROI.getBoundsX(), childROI.getBoundsY(), childROI.getBoundsWidth(), childROI.getBoundsHeight())) || childObject.hasChildren())
                painted = paintObject(childObject, paintChildren, g, boundsDisplayed, overlayOptions, selectionModel, downsample) | painted;
        }
    }
    return painted;
}

Example 3 with LineROI

use of qupath.lib.roi.LineROI in project qupath by qupath.

the class IJTools method convertToIJRoi.

/**
 * Convert a QuPath ROI to an ImageJ Roi.
 * @param <T>
 * @param pathROI
 * @param xOrigin x-origin indicating relationship of ImagePlus to the original image, as stored in ImageJ Calibration object
 * @param yOrigin y-origin indicating relationship of ImagePlus to the original image, as stored in ImageJ Calibration object
 * @param downsampleFactor downsample factor at which the ImagePlus was extracted from the full-resolution image
 * @return
 */
public static <T extends PathImage<ImagePlus>> Roi convertToIJRoi(ROI pathROI, double xOrigin, double yOrigin, double downsampleFactor) {
    if (pathROI instanceof PolygonROI)
        return ROIConverterIJ.convertToPolygonROI((PolygonROI) pathROI, xOrigin, yOrigin, downsampleFactor);
    if (pathROI instanceof RectangleROI)
        return ROIConverterIJ.getRectangleROI((RectangleROI) pathROI, xOrigin, yOrigin, downsampleFactor);
    if (pathROI instanceof EllipseROI)
        return ROIConverterIJ.convertToOvalROI((EllipseROI) pathROI, xOrigin, yOrigin, downsampleFactor);
    if (pathROI instanceof LineROI)
        return ROIConverterIJ.convertToLineROI((LineROI) pathROI, xOrigin, yOrigin, downsampleFactor);
    if (pathROI instanceof PolylineROI)
        return ROIConverterIJ.convertToPolygonROI((PolylineROI) pathROI, xOrigin, yOrigin, downsampleFactor);
    if (pathROI instanceof PointsROI)
        return ROIConverterIJ.convertToPointROI((PointsROI) pathROI, xOrigin, yOrigin, downsampleFactor);
    // If we have any other kind of shape, create a general shape roi
    if (pathROI != null && pathROI.isArea()) {
        // TODO: Deal with non-AWT area ROIs!
        Shape shape = RoiTools.getArea(pathROI);
        // "scaleX", "shearY", "shearX", "scaleY", "translateX", "translateY"
        shape = new AffineTransform(1.0 / downsampleFactor, 0, 0, 1.0 / downsampleFactor, xOrigin, yOrigin).createTransformedShape(shape);
        return ROIConverterIJ.setIJRoiProperties(new ShapeRoi(shape), pathROI);
    }
    // TODO: Integrate ROI not supported exception...?
    return null;
}

Example 4 with LineROI

use of qupath.lib.roi.LineROI in project qupath by qupath.

the class ImageJMacroRunner method runMacro.

static void runMacro(final ParameterList params, final ImageData<BufferedImage> imageData, final ImageDisplay imageDisplay, final PathObject pathObject, final String macroText) {
    // Don't try if interrupted
    if (Thread.currentThread().isInterrupted()) {
        logger.warn("Skipping macro for {} - thread interrupted", pathObject);
        return;
    }
    PathImage<ImagePlus> pathImage;
    // Extract parameters
    double downsampleFactor = params.getDoubleParameterValue("downsampleFactor");
    boolean sendROI = params.getBooleanParameterValue("sendROI");
    boolean sendOverlay = params.getBooleanParameterValue("sendOverlay");
    ROI pathROI = pathObject.getROI();
    ImageDisplay imageDisplay2 = params.containsKey("useTransform") && Boolean.TRUE.equals(params.getBooleanParameterValue("useTransform")) ? imageDisplay : null;
    ImageServer<BufferedImage> server = imageDisplay2 == null || imageDisplay2.availableChannels().isEmpty() ? imageData.getServer() : ChannelDisplayTransformServer.createColorTransformServer(imageData.getServer(), imageDisplay.availableChannels());
    RegionRequest region = RegionRequest.createInstance(imageData.getServer().getPath(), downsampleFactor, pathROI);
    // Check the size of the region to extract - abort if it is too large of if ther isn't enough RAM
    try {
        IJTools.isMemorySufficient(region, imageData);
    } catch (Exception e1) {
        Dialogs.showErrorMessage("ImageJ macro error", e1.getMessage());
        return;
    }
    try {
        if (sendOverlay)
            pathImage = IJExtension.extractROIWithOverlay(server, pathObject, imageData.getHierarchy(), region, sendROI, null);
        else
            pathImage = IJExtension.extractROI(server, pathObject, region, sendROI);
    } catch (IOException e) {
        logger.error("Unable to extract image region " + region, e);
        return;
    }
    // IJHelpers.getImageJInstance();
    // ImageJ ij = IJHelpers.getImageJInstance();
    // if (ij != null && WindowManager.getIDList() == null)
    // ij.setVisible(false);
    // Determine a sensible argument to pass
    String argument;
    if (pathObject instanceof TMACoreObject || !pathObject.hasROI())
        argument = pathObject.getDisplayedName();
    else
        argument = String.format("Region (%d, %d, %d, %d)", region.getX(), region.getY(), region.getWidth(), region.getHeight());
    // Check if we have an image already - if so, we need to be more cautious so we don't accidentally use it...
    // boolean hasImage = WindowManager.getCurrentImage() != null;
    // Actually run the macro
    final ImagePlus imp = pathImage.getImage();
    imp.setProperty("QuPath region", argument);
    WindowManager.setTempCurrentImage(imp);
    // Ensure we've requested an instance, since this also loads any required extra plugins
    IJExtension.getImageJInstance();
    // TODO: Pay attention to how threading should be done... I think Swing EDT ok?
    try {
        // SwingUtilities.invokeAndWait(() -> {
        boolean cancelled = false;
        ImagePlus impResult = null;
        try {
            IJ.redirectErrorMessages();
            Interpreter interpreter = new Interpreter();
            impResult = interpreter.runBatchMacro(macroText, imp);
            // If we had an error, return
            if (interpreter.wasError()) {
                Thread.currentThread().interrupt();
                return;
            }
            // Get the resulting image, if available
            if (impResult == null)
                impResult = WindowManager.getCurrentImage();
        } catch (RuntimeException e) {
            logger.error(e.getLocalizedMessage());
            // DisplayHelpers.showErrorMessage("ImageJ macro error", e.getLocalizedMessage());
            Thread.currentThread().interrupt();
            cancelled = true;
        } finally {
            // IJ.runMacro(macroText, argument);
            WindowManager.setTempCurrentImage(null);
        // IJ.run("Close all");
        }
        if (cancelled)
            return;
        // Get the current image when the macro has finished - which may or may not be the same as the original
        if (impResult == null)
            impResult = imp;
        boolean changes = false;
        if (params.getBooleanParameterValue("clearObjects") && pathObject.hasChildren()) {
            pathObject.clearPathObjects();
            changes = true;
        }
        if (params.getBooleanParameterValue("getROI") && impResult.getRoi() != null) {
            Roi roi = impResult.getRoi();
            Calibration cal = impResult.getCalibration();
            PathObject pathObjectNew = roi == null ? null : IJTools.convertToAnnotation(roi, cal.xOrigin, cal.yOrigin, downsampleFactor, region.getPlane());
            if (pathObjectNew != null) {
                // If necessary, trim any returned annotation
                if (pathROI != null && !(pathROI instanceof RectangleROI) && pathObjectNew.isAnnotation() && RoiTools.isShapeROI(pathROI) && RoiTools.isShapeROI(pathObjectNew.getROI())) {
                    ROI roiNew = RoiTools.combineROIs(pathROI, pathObjectNew.getROI(), CombineOp.INTERSECT);
                    ((PathAnnotationObject) pathObjectNew).setROI(roiNew);
                }
                // Only add if we have something
                if (pathObjectNew.getROI() instanceof LineROI || !pathObjectNew.getROI().isEmpty()) {
                    pathObject.addPathObject(pathObjectNew);
                    // imageData.getHierarchy().addPathObject(IJHelpers.convertToPathObject(imp, imageData.getServer(), imp.getRoi(), downsampleFactor, false), true);
                    changes = true;
                }
            }
        }
        boolean exportAsDetection = ((String) params.getChoiceParameterValue("getOverlayAs")).equals("Detections") ? true : false;
        if (params.getBooleanParameterValue("getOverlay") && impResult.getOverlay() != null) {
            var overlay = impResult.getOverlay();
            List<PathObject> childObjects = QuPath_Send_Overlay_to_QuPath.createObjectsFromROIs(imp, Arrays.asList(overlay.toArray()), downsampleFactor, exportAsDetection, true, region.getPlane());
            if (!childObjects.isEmpty()) {
                pathObject.addPathObjects(childObjects);
                changes = true;
            }
        // for (Roi roi : impResult.getOverlay().toArray()) {
        // pathObject.addPathObject(IJTools.convertToPathObject(imp, imageData.getServer(), roi, downsampleFactor, true));
        // changes = true;
        // }
        }
        if (changes) {
            Platform.runLater(() -> imageData.getHierarchy().fireHierarchyChangedEvent(null));
        }
    // });
    } catch (Exception e) {
        // TODO Auto-generated catch block
        e.printStackTrace();
    }
}