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Example 36 with TextWindow

use of ij.text.TextWindow in project GDSC-SMLM by aherbert.

the class BenchmarkFilterAnalysis method createSensitivityWindow.

private void createSensitivityWindow() {
    if (isHeadless) {
        IJ.log(createSensitivityHeader());
    } else {
        if (sensitivityWindow == null || !sensitivityWindow.isShowing()) {
            String header = createSensitivityHeader();
            sensitivityWindow = new TextWindow(TITLE + " Sensitivity", header, "", 900, 300);
        }
    }
}
Also used : TextWindow(ij.text.TextWindow) BufferedTextWindow(gdsc.core.ij.BufferedTextWindow)

Example 37 with TextWindow

use of ij.text.TextWindow in project GDSC-SMLM by aherbert.

the class BenchmarkFilterAnalysis method createGAWindow.

private void createGAWindow() {
    if (isHeadless) {
        String header = createResultsHeader(false);
        header += "\tIteration";
        IJ.log(header);
    } else {
        if (gaWindow == null || !gaWindow.isShowing()) {
            String header = createResultsHeader(false);
            header += "\tIteration";
            gaWindow = new TextWindow(TITLE + " Evolution", header, "", 900, 300);
        }
        if (clearTables)
            gaWindow.getTextPanel().clear();
    }
}
Also used : TextWindow(ij.text.TextWindow) BufferedTextWindow(gdsc.core.ij.BufferedTextWindow)

Example 38 with TextWindow

use of ij.text.TextWindow in project GDSC-SMLM by aherbert.

the class MeanVarianceTest method run.

/*
	 * (non-Javadoc)
	 * 
	 * @see ij.plugin.PlugIn#run(java.lang.String)
	 */
public void run(String arg) {
    SMLMUsageTracker.recordPlugin(this.getClass(), arg);
    if (Utils.isExtraOptions()) {
        ImagePlus imp = WindowManager.getCurrentImage();
        if (imp.getStackSize() > 1) {
            GenericDialog gd = new GenericDialog(TITLE);
            gd.addMessage("Perform single image analysis on the current image?");
            gd.addNumericField("Bias", _bias, 0);
            gd.showDialog();
            if (gd.wasCanceled())
                return;
            singleImage = true;
            _bias = Math.abs(gd.getNextNumber());
        } else {
            IJ.error(TITLE, "Single-image mode requires a stack");
            return;
        }
    }
    List<ImageSample> images;
    String inputDirectory = "";
    if (singleImage) {
        IJ.showStatus("Loading images...");
        images = getImages();
        if (images.size() == 0) {
            IJ.error(TITLE, "Not enough images for analysis");
            return;
        }
    } else {
        inputDirectory = IJ.getDirectory("Select image series ...");
        if (inputDirectory == null)
            return;
        SeriesOpener series = new SeriesOpener(inputDirectory, false, 0);
        series.setVariableSize(true);
        if (series.getNumberOfImages() < 3) {
            IJ.error(TITLE, "Not enough images in the selected directory");
            return;
        }
        if (!IJ.showMessageWithCancel(TITLE, String.format("Analyse %d images, first image:\n%s", series.getNumberOfImages(), series.getImageList()[0]))) {
            return;
        }
        IJ.showStatus("Loading images");
        images = getImages(series);
        if (images.size() < 3) {
            IJ.error(TITLE, "Not enough images for analysis");
            return;
        }
        if (images.get(0).exposure != 0) {
            IJ.error(TITLE, "First image in series must have exposure 0 (Bias image)");
            return;
        }
    }
    boolean emMode = (arg != null && arg.contains("em"));
    GenericDialog gd = new GenericDialog(TITLE);
    gd.addMessage("Set the output options:");
    gd.addCheckbox("Show_table", showTable);
    gd.addCheckbox("Show_charts", showCharts);
    if (emMode) {
        // Ask the user for the camera gain ...
        gd.addMessage("Estimating the EM-gain requires the camera gain without EM readout enabled");
        gd.addNumericField("Camera_gain (ADU/e-)", cameraGain, 4);
    }
    gd.showDialog();
    if (gd.wasCanceled())
        return;
    showTable = gd.getNextBoolean();
    showCharts = gd.getNextBoolean();
    if (emMode) {
        cameraGain = gd.getNextNumber();
    }
    IJ.showStatus("Computing mean & variance");
    final double nImages = images.size();
    for (int i = 0; i < images.size(); i++) {
        IJ.showStatus(String.format("Computing mean & variance %d/%d", i + 1, images.size()));
        images.get(i).compute(singleImage, i / nImages, (i + 1) / nImages);
    }
    IJ.showProgress(1);
    IJ.showStatus("Computing results");
    // Allow user to input multiple bias images
    int start = 0;
    Statistics biasStats = new Statistics();
    Statistics noiseStats = new Statistics();
    final double bias;
    if (singleImage) {
        bias = _bias;
    } else {
        while (start < images.size()) {
            ImageSample sample = images.get(start);
            if (sample.exposure == 0) {
                biasStats.add(sample.means);
                for (PairSample pair : sample.samples) {
                    noiseStats.add(pair.variance);
                }
                start++;
            } else
                break;
        }
        bias = biasStats.getMean();
    }
    // Get the mean-variance data
    int total = 0;
    for (int i = start; i < images.size(); i++) total += images.get(i).samples.size();
    if (showTable && total > 2000) {
        gd = new GenericDialog(TITLE);
        gd.addMessage("Table output requires " + total + " entries.\n \nYou may want to disable the table.");
        gd.addCheckbox("Show_table", showTable);
        gd.showDialog();
        if (gd.wasCanceled())
            return;
        showTable = gd.getNextBoolean();
    }
    TextWindow results = (showTable) ? createResultsWindow() : null;
    double[] mean = new double[total];
    double[] variance = new double[mean.length];
    Statistics gainStats = (singleImage) ? new StoredDataStatistics(total) : new Statistics();
    final WeightedObservedPoints obs = new WeightedObservedPoints();
    for (int i = (singleImage) ? 0 : start, j = 0; i < images.size(); i++) {
        StringBuilder sb = (showTable) ? new StringBuilder() : null;
        ImageSample sample = images.get(i);
        for (PairSample pair : sample.samples) {
            if (j % 16 == 0)
                IJ.showProgress(j, total);
            mean[j] = pair.getMean();
            variance[j] = pair.variance;
            // Gain is in ADU / e
            double gain = variance[j] / (mean[j] - bias);
            gainStats.add(gain);
            obs.add(mean[j], variance[j]);
            if (emMode) {
                gain /= (2 * cameraGain);
            }
            if (showTable) {
                sb.append(sample.title).append("\t");
                sb.append(sample.exposure).append("\t");
                sb.append(pair.slice1).append("\t");
                sb.append(pair.slice2).append("\t");
                sb.append(IJ.d2s(pair.mean1, 2)).append("\t");
                sb.append(IJ.d2s(pair.mean2, 2)).append("\t");
                sb.append(IJ.d2s(mean[j], 2)).append("\t");
                sb.append(IJ.d2s(variance[j], 2)).append("\t");
                sb.append(Utils.rounded(gain, 4)).append("\n");
            }
            j++;
        }
        if (showTable)
            results.append(sb.toString());
    }
    IJ.showProgress(1);
    if (singleImage) {
        StoredDataStatistics stats = (StoredDataStatistics) gainStats;
        Utils.log(TITLE);
        if (emMode) {
            double[] values = stats.getValues();
            MathArrays.scaleInPlace(0.5, values);
            stats = new StoredDataStatistics(values);
        }
        if (showCharts) {
            // Plot the gain over time
            String title = TITLE + " Gain vs Frame";
            Plot2 plot = new Plot2(title, "Slice", "Gain", Utils.newArray(gainStats.getN(), 1, 1.0), stats.getValues());
            PlotWindow pw = Utils.display(title, plot);
            // Show a histogram
            String label = String.format("Mean = %s, Median = %s", Utils.rounded(stats.getMean()), Utils.rounded(stats.getMedian()));
            int id = Utils.showHistogram(TITLE, stats, "Gain", 0, 1, 100, true, label);
            if (Utils.isNewWindow()) {
                Point point = pw.getLocation();
                point.x = pw.getLocation().x;
                point.y += pw.getHeight();
                WindowManager.getImage(id).getWindow().setLocation(point);
            }
        }
        Utils.log("Single-image mode: %s camera", (emMode) ? "EM-CCD" : "Standard");
        final double gain = stats.getMedian();
        if (emMode) {
            final double totalGain = gain;
            final double emGain = totalGain / cameraGain;
            Utils.log("  Gain = 1 / %s (ADU/e-)", Utils.rounded(cameraGain, 4));
            Utils.log("  EM-Gain = %s", Utils.rounded(emGain, 4));
            Utils.log("  Total Gain = %s (ADU/e-)", Utils.rounded(totalGain, 4));
        } else {
            cameraGain = gain;
            Utils.log("  Gain = 1 / %s (ADU/e-)", Utils.rounded(cameraGain, 4));
        }
    } else {
        IJ.showStatus("Computing fit");
        // Sort
        int[] indices = rank(mean);
        mean = reorder(mean, indices);
        variance = reorder(variance, indices);
        // Compute optimal coefficients.
        // a - b x
        final double[] init = { 0, 1 / gainStats.getMean() };
        final PolynomialCurveFitter fitter = PolynomialCurveFitter.create(2).withStartPoint(init);
        final double[] best = fitter.fit(obs.toList());
        // Construct the polynomial that best fits the data.
        final PolynomialFunction fitted = new PolynomialFunction(best);
        if (showCharts) {
            // Plot mean verses variance. Gradient is gain in ADU/e.
            String title = TITLE + " results";
            Plot2 plot = new Plot2(title, "Mean", "Variance");
            double[] xlimits = Maths.limits(mean);
            double[] ylimits = Maths.limits(variance);
            double xrange = (xlimits[1] - xlimits[0]) * 0.05;
            if (xrange == 0)
                xrange = 0.05;
            double yrange = (ylimits[1] - ylimits[0]) * 0.05;
            if (yrange == 0)
                yrange = 0.05;
            plot.setLimits(xlimits[0] - xrange, xlimits[1] + xrange, ylimits[0] - yrange, ylimits[1] + yrange);
            plot.setColor(Color.blue);
            plot.addPoints(mean, variance, Plot2.CROSS);
            plot.setColor(Color.red);
            plot.addPoints(new double[] { mean[0], mean[mean.length - 1] }, new double[] { fitted.value(mean[0]), fitted.value(mean[mean.length - 1]) }, Plot2.LINE);
            Utils.display(title, plot);
        }
        final double avBiasNoise = Math.sqrt(noiseStats.getMean());
        Utils.log(TITLE);
        Utils.log("  Directory = %s", inputDirectory);
        Utils.log("  Bias = %s +/- %s (ADU)", Utils.rounded(bias, 4), Utils.rounded(avBiasNoise, 4));
        Utils.log("  Variance = %s + %s * mean", Utils.rounded(best[0], 4), Utils.rounded(best[1], 4));
        if (emMode) {
            final double emGain = best[1] / (2 * cameraGain);
            // Noise is standard deviation of the bias image divided by the total gain (in ADU/e-)
            final double totalGain = emGain * cameraGain;
            Utils.log("  Read Noise = %s (e-) [%s (ADU)]", Utils.rounded(avBiasNoise / totalGain, 4), Utils.rounded(avBiasNoise, 4));
            Utils.log("  Gain = 1 / %s (ADU/e-)", Utils.rounded(1 / cameraGain, 4));
            Utils.log("  EM-Gain = %s", Utils.rounded(emGain, 4));
            Utils.log("  Total Gain = %s (ADU/e-)", Utils.rounded(totalGain, 4));
        } else {
            // Noise is standard deviation of the bias image divided by the gain (in ADU/e-)
            cameraGain = best[1];
            final double readNoise = avBiasNoise / cameraGain;
            Utils.log("  Read Noise = %s (e-) [%s (ADU)]", Utils.rounded(readNoise, 4), Utils.rounded(readNoise * cameraGain, 4));
            Utils.log("  Gain = 1 / %s (ADU/e-)", Utils.rounded(1 / cameraGain, 4));
        }
    }
    IJ.showStatus("");
}
Also used : StoredDataStatistics(gdsc.core.utils.StoredDataStatistics) PlotWindow(ij.gui.PlotWindow) PolynomialFunction(org.apache.commons.math3.analysis.polynomials.PolynomialFunction) SeriesOpener(gdsc.smlm.ij.utils.SeriesOpener) Plot2(ij.gui.Plot2) Point(java.awt.Point) ImagePlus(ij.ImagePlus) StoredDataStatistics(gdsc.core.utils.StoredDataStatistics) Statistics(gdsc.core.utils.Statistics) Point(java.awt.Point) PolynomialCurveFitter(org.apache.commons.math3.fitting.PolynomialCurveFitter) WeightedObservedPoints(org.apache.commons.math3.fitting.WeightedObservedPoints) TextWindow(ij.text.TextWindow) GenericDialog(ij.gui.GenericDialog)

Example 39 with TextWindow

use of ij.text.TextWindow in project GDSC-SMLM by aherbert.

the class SummariseResults method createSummaryTable.

private void createSummaryTable() {
    if (summary == null || !summary.isShowing()) {
        StringBuilder sb = new StringBuilder("Dataset\tN\tFrames\tTime\tMemory\tBounds\tnm/pixel\tGain\tms/frame");
        for (String statName : new String[] { "Precision (nm)", "SNR" }) {
            sb.append("\tAv ").append(statName);
            sb.append("\tMedian ").append(statName);
            sb.append("\tMin ").append(statName);
            sb.append("\tMax ").append(statName);
        }
        summary = new TextWindow("Peak Results Summary", sb.toString(), "", 800, 300);
        summary.setVisible(true);
    }
    // This could be optional but at current there is no dialog and it seems unnecessary
    clearSummaryTable();
}
Also used : TextWindow(ij.text.TextWindow)

Example 40 with TextWindow

use of ij.text.TextWindow in project GDSC-SMLM by aherbert.

the class PCPALMAnalysis method createResultsTable.

private void createResultsTable() {
    if (resultsTable == null || !resultsTable.isVisible()) {
        StringBuilder sb = new StringBuilder();
        sb.append("ID\t");
        sb.append("Image Source\t");
        sb.append("X\t");
        sb.append("X %\t");
        sb.append("Y\t");
        sb.append("Y %\t");
        sb.append("Width\t");
        sb.append("Width %\t");
        sb.append("Height\t");
        sb.append("Height %\t");
        sb.append("N\t");
        sb.append("PeakDensity (um^-2)\t");
        sb.append("nm/pixel\t");
        sb.append("Binary\t");
        resultsTable = new TextWindow(TITLE, sb.toString(), (String) null, 800, 300);
    }
}
Also used : TextWindow(ij.text.TextWindow)

Aggregations

TextWindow (ij.text.TextWindow)61 Point (java.awt.Point)11 BufferedTextWindow (uk.ac.sussex.gdsc.core.ij.BufferedTextWindow)11 BufferedTextWindow (gdsc.core.ij.BufferedTextWindow)7 ArrayList (java.util.ArrayList)6 PointPair (uk.ac.sussex.gdsc.core.match.PointPair)5 IJ (ij.IJ)4 ImagePlus (ij.ImagePlus)4 LinkedList (java.util.LinkedList)4 Coordinate (uk.ac.sussex.gdsc.core.match.Coordinate)4 ImageROIPainter (gdsc.smlm.ij.utils.ImageROIPainter)3 List (java.util.List)3 PeakResultPoint (uk.ac.sussex.gdsc.smlm.results.PeakResultPoint)3 Coordinate (gdsc.core.match.Coordinate)2 MatchResult (gdsc.core.match.MatchResult)2 PointPair (gdsc.core.match.PointPair)2 TIntHashSet (gnu.trove.set.hash.TIntHashSet)2 GenericDialog (ij.gui.GenericDialog)2 ImageWindow (ij.gui.ImageWindow)2 PlotWindow (ij.gui.PlotWindow)2