use of ini.trakem2.vector.VectorString3D in project TrakEM2 by trakem2.
the class Pipe method measure.
/*
// debug ---------------
static private void showShape(final Shape shape) {
Area area = new Area(shape);
Rectangle b = area.getBounds();
AffineTransform trans = new AffineTransform();
trans.translate(-b.x, -b.y);
area = area.createTransformedArea(trans);
ij.process.ByteProcessor bp = new ij.process.ByteProcessor(b.width, b.height);
ij.gui.ShapeRoi sr = new ij.gui.ShapeRoi(area);
ij.ImagePlus imp = new ij.ImagePlus("pipe area", bp);
imp.setRoi(sr);
bp.setValue(255);
sr.drawPixels(bp);
imp.show();
}
*/
@Override
public ResultsTable measure(ResultsTable rt) {
// reload
if (-1 == n_points)
setupForDisplay();
if (0 == n_points)
return rt;
if (null == rt)
rt = Utils.createResultsTable("Pipe results", new String[] { "id", "length", "name-id" });
// measure length
double len = 0;
final Calibration cal = layer_set.getCalibration();
if (n_points > 1) {
final VectorString3D vs = asVectorString3D();
vs.calibrate(cal);
// no resampling
len = vs.computeLength();
}
rt.incrementCounter();
rt.addLabel("units", cal.getUnit());
rt.addValue(0, this.id);
rt.addValue(1, len);
rt.addValue(2, getNameId());
return rt;
}
use of ini.trakem2.vector.VectorString3D in project TrakEM2 by trakem2.
the class Pipe method asVectorString3D.
/**
* Returns a non-calibrated VectorString3D.
*/
@Override
public synchronized VectorString3D asVectorString3D() {
// local pointers, since they may be transformed
int n_points = this.n_points;
double[][] p = this.p;
// double[][] p_r = this.p_r;
// double[][] p_l = this.p_l;
double[][] p_i = this.p_i;
// double[] p_width = this.p_width;
double[] p_width_i = this.p_width_i;
if (!this.at.isIdentity()) {
final Object[] ob = getTransformedData();
p = (double[][]) ob[0];
n_points = p[0].length;
// p_l = (double[][])ob[1];
// p_r = (double[][])ob[2];
p_i = (double[][]) ob[3];
// p_width = (double[])ob[4];
p_width_i = (double[]) ob[5];
}
final int n = p_i[0].length;
final int mm = n_points;
final double[] z_values = new double[n];
final int interval_points = n / (mm - 1);
double z_val = 0;
double z_val_next = 0;
double z_diff = 0;
int c = 0;
double delta = 0;
for (int j = 0; j < mm - 1; j++) {
z_val = layer_set.getLayer(p_layer[j]).getZ();
z_val_next = layer_set.getLayer(p_layer[j + 1]).getZ();
z_diff = z_val_next - z_val;
delta = z_diff / interval_points;
z_values[c] = (0 == j ? z_val : z_values[c - 1]) + delta;
for (int k = 1; k < interval_points; k++) {
c++;
z_values[c] = z_values[c - 1] + delta;
}
c++;
}
// setting last point
z_values[n - 1] = layer_set.getLayer(p_layer[mm - 1]).getZ();
final double[] px = p_i[0];
final double[] py = p_i[1];
final double[] pz = z_values;
VectorString3D vs = null;
try {
vs = new VectorString3D(px, py, pz, false);
vs.addDependent(p_width_i);
} catch (final Exception e) {
IJError.print(e);
}
return vs;
}
use of ini.trakem2.vector.VectorString3D in project TrakEM2 by trakem2.
the class Polyline method asVectorString3D.
/**
* Returns a non-calibrated VectorString3D.
*/
@Override
public synchronized VectorString3D asVectorString3D() {
// local pointers, since they may be transformed
int n_points = this.n_points;
double[][] p = this.p;
if (!this.at.isIdentity()) {
final Object[] ob = getTransformedData();
p = (double[][]) ob[0];
n_points = p[0].length;
}
final double[] z_values = new double[n_points];
for (int i = 0; i < n_points; i++) {
z_values[i] = layer_set.getLayer(p_layer[i]).getZ();
}
final double[] px = p[0];
final double[] py = p[1];
final double[] pz = z_values;
VectorString3D vs = null;
try {
vs = new VectorString3D(px, py, pz, false);
} catch (final Exception e) {
IJError.print(e);
}
return vs;
}
use of ini.trakem2.vector.VectorString3D in project TrakEM2 by trakem2.
the class Compare method variabilityAnalysis.
/**
* @param reference_project If null, then the first one found in the Project.getProjects() lists is used.
* @param regex A String (can be null) to filter objects by, to limit what gets processed.
* If regex is not null, then only ProjectThing nodes with the matching regex are analyzed (shallow: none of their children are questioned, but pipes will be built from them all).
* @param generate_plots Whether to generate the variability plots at all.
* @param show_plots If generate_plots, whether to show the plots in a stack image window or to save them.
* @param show_3D Whether to show any 3D data.
* @param show_condensed_3D If show_3D, whether to show the condensed vector strings, i.e. the "average" pipes.
* @param show_sources_3D If show_3D, whether to show the source pipes from which the condensed vector string was generated.
* @param sources_color_table Which colors to give to the pipes of which Project.
* @param show_envelope_3D If show_3D, whether to generate the variability envelope.
* @param envelope_alpha If show_envelope_3D, the envelope takes an alpha value between 0 (total transparency) and 1 (total opacity)
* @param delta_envelope The delta to resample the envelope to. When smaller than or equal to 1, no envelope resampling occurs.
* @param show_axes_3D If show_3D, whether to display the reference axes as well.
* @param heat_map If show_3D, whether to color the variability with a Fire LUT.
* If not show_condensed_3D, then the variability is shown in color-coded 3D spheres placed at the entry point to the neuropile.
* @param map_condensed If not null, all VectorString3D are put into this map.
* @param projects The projects to use.
*/
public static Bureaucrat variabilityAnalysis(final Project reference_project, final String regex, final String[] ignore, final boolean show_cata_dialog, final boolean generate_plots, final boolean show_plots, final String plot_dir_, final boolean show_3D, final boolean show_condensed_3D, final boolean show_sources_3D, final Map<Project, Color> sources_color_table, final boolean show_envelope_3D, final float envelope_alpha, final double delta_envelope, final int envelope_type, final boolean show_axes_3D, final boolean heat_map, final Map<String, VectorString3D> map_condensed, final Project[] projects) {
// gather all open projects
final Project[] p = null == projects ? Project.getProjects().toArray(new Project[0]) : projects;
// make the reference_project be the first in the array
if (null != reference_project && reference_project != p[0]) {
for (int i = 0; i < p.length; i++) {
if (reference_project == p[i]) {
p[i] = p[0];
p[0] = reference_project;
break;
}
}
}
final Worker worker = new Worker("Comparing all to all") {
@Override
public void run() {
startedWorking();
try {
Utils.log2("Asking for CATAParameters...");
final CATAParameters cp = new CATAParameters();
cp.regex = regex;
cp.delta_envelope = delta_envelope;
cp.envelope_type = envelope_type;
if (show_cata_dialog && !cp.setup(false, regex, true, true)) {
finishedWorking();
return;
}
// so source points are stored in VectorString3D for each resampled and interpolated point
cp.with_source = true;
// Store a series of results, depending on options
final HashMap<String, Display3D> results = new HashMap<String, Display3D>();
String plot_dir = plot_dir_;
if (generate_plots && !show_plots) {
// Save plots
if (null == plot_dir) {
final DirectoryChooser dc = new DirectoryChooser("Choose plots directory");
plot_dir = dc.getDirectory();
if (null == plot_dir) {
finishedWorking();
return;
}
}
if (IJ.isWindows())
plot_dir = plot_dir.replace('\\', '/');
if (!plot_dir.endsWith("/"))
plot_dir += "/";
}
Utils.log2("Gathering chains...");
// Gather chains that do not match the ignore regexes
// will transform them as well to the reference found in the first project in the p array
Object[] ob = gatherChains(p, cp, ignore);
ArrayList<Chain> chains = (ArrayList<Chain>) ob[0];
// to keep track of each project's chains
final ArrayList[] p_chains = (ArrayList[]) ob[1];
ob = null;
if (null == chains) {
finishedWorking();
return;
}
Utils.log2("Collecting bundles...");
final HashMap<Project, HashMap<String, VectorString3D>> axes = new HashMap<Project, HashMap<String, VectorString3D>>();
// Sort out into groups by unique names of lineage bundles
final HashMap<String, ArrayList<Chain>> bundles = new HashMap<String, ArrayList<Chain>>();
for (final Chain chain : chains) {
String title = chain.getCellTitle();
final String t = title.toLowerCase();
// unnamed
if (0 == t.indexOf('[') || 0 == t.indexOf('#'))
continue;
Utils.log("Accepting " + title);
title = title.substring(0, title.indexOf(' '));
// lineage bundle instance chains
ArrayList<Chain> bc = bundles.get(title);
if (null == bc) {
bc = new ArrayList<Chain>();
bundles.put(title, bc);
}
bc.add(chain);
}
Utils.log2("Found " + bundles.size() + " bundles.");
chains = null;
if (null != cp.regex && show_axes_3D && axes.size() < 3) {
// Must find the Mushroom Body lobes separately
final String cp_regex = cp.regex;
cp.regex = "mb";
final Object[] o = gatherChains(p, cp, ignore);
final ArrayList<Chain> lobes = (ArrayList<Chain>) o[0];
Utils.logAll("Found " + lobes.size() + " chains for lobes");
for (final Chain chain : lobes) {
final String t = chain.getCellTitle().toLowerCase();
if (-1 != t.indexOf("peduncle") || -1 != t.indexOf("medial lobe") || -1 != t.indexOf("dorsal lobe")) {
Utils.logAll("adding " + t);
final Project pr = chain.pipes.get(0).getProject();
HashMap<String, VectorString3D> m = axes.get(pr);
if (null == m) {
m = new HashMap<String, VectorString3D>();
axes.put(pr, m);
}
m.put(t, chain.vs);
continue;
}
}
cp.regex = cp_regex;
} else {
Utils.logAll("Not: cp.regex = " + cp.regex + " show_axes_3D = " + show_axes_3D + " axes.size() = " + axes.size());
}
final HashMap<String, VectorString3D> condensed = new HashMap<String, VectorString3D>();
Utils.log2("Condensing each bundle...");
// Condense each into a single VectorString3D
for (final Map.Entry<String, ArrayList<Chain>> entry : bundles.entrySet()) {
final ArrayList<Chain> bc = entry.getValue();
if (bc.size() < 2) {
Utils.log2("Skipping single: " + entry.getKey());
continue;
}
final VectorString3D[] vs = new VectorString3D[bc.size()];
for (int i = 0; i < vs.length; i++) vs[i] = bc.get(i).vs;
final VectorString3D c = condense(cp, vs, this);
c.setCalibration(p[0].getRootLayerSet().getCalibrationCopy());
condensed.put(entry.getKey(), c);
if (this.hasQuitted())
return;
}
// Store:
if (null != map_condensed) {
map_condensed.putAll(condensed);
}
if (generate_plots) {
Utils.log2("Plotting stdDev for each condensed bundle...");
// Y axis: the stdDev at each point, computed from the group of points that contribute to each
for (final Map.Entry<String, VectorString3D> e : condensed.entrySet()) {
final String name = e.getKey();
final VectorString3D c = e.getValue();
final Plot plot = makePlot(cp, name, c);
// FAILS//plot.addLabel(10, cp.plot_height-5, name); // must be added after setting size
if (show_plots)
plot.show();
else if (null != plot_dir)
new FileSaver(plot.getImagePlus()).saveAsPng(plot_dir + name.replace('/', '-') + ".png");
}
}
if (show_3D) {
final HashMap<String, Color> heat_table = new HashMap<String, Color>();
if (heat_map || show_envelope_3D) {
// Create a Fire LUT
final ImagePlus lutimp = new ImagePlus("lut", new ByteProcessor(4, 4));
IJ.run(lutimp, "Fire", "");
final IndexColorModel icm = (IndexColorModel) lutimp.getProcessor().getColorModel();
final byte[] reds = new byte[256];
final byte[] greens = new byte[256];
final byte[] blues = new byte[256];
icm.getReds(reds);
icm.getGreens(greens);
icm.getBlues(blues);
final List<String> names = new ArrayList<String>(bundles.keySet());
Collections.sort(names);
// find max stdDev
double max = 0;
final HashMap<String, Double> heats = new HashMap<String, Double>();
for (final String name : names) {
final VectorString3D vs_merged = condensed.get(name);
if (null == vs_merged) {
Utils.logAll("WARNING could not find a condensed pipe for " + name);
continue;
}
final double[] stdDev = vs_merged.getStdDevAtEachPoint();
// double avg = 0;
// for (int i=0; i<stdDev.length; i++) avg += stdDev[i];
// avg = avg/stdDev.length;
Arrays.sort(stdDev);
// median is more representative than average
final double median = stdDev[stdDev.length / 2];
if (max < median)
max = median;
heats.put(name, median);
}
for (final Map.Entry<String, Double> e : heats.entrySet()) {
final String name = e.getKey();
final double median = e.getValue();
// scale between 0 and max to get a Fire LUT color:
int index = (int) ((median / max) * 255);
if (index > 255)
index = 255;
final Color color = new Color(0xff & reds[index], 0xff & greens[index], 0xff & blues[index]);
Utils.log2(new StringBuilder(name).append('\t').append(median).append('\t').append(reds[index]).append('\t').append(greens[index]).append('\t').append(blues[index]).toString());
heat_table.put(name, color);
}
}
final LayerSet common_ls = new LayerSet(p[0], -1, "Common", 10, 10, 0, 0, 0, 512, 512, false, 2, new AffineTransform());
final Display3D d3d = Display3D.get(common_ls);
float env_alpha = envelope_alpha;
if (env_alpha < 0) {
Utils.log2("WARNING envelope_alpha is invalid: " + envelope_alpha + "\n Using 0.4f instead");
env_alpha = 0.4f;
} else if (env_alpha > 1)
env_alpha = 1.0f;
for (final String name : bundles.keySet()) {
final ArrayList<Chain> bc = bundles.get(name);
final VectorString3D vs_merged = condensed.get(name);
if (null == vs_merged) {
Utils.logAll("WARNING: could not find a condensed vs for " + name);
continue;
}
if (show_sources_3D) {
if (null != sources_color_table) {
final HashSet<String> titles = new HashSet<String>();
for (final Chain chain : bc) {
final Color c = sources_color_table.get(chain.getRoot().getProject());
final String title = chain.getCellTitle();
String t = title;
int i = 2;
while (titles.contains(t)) {
t = title + "-" + i;
i += 1;
}
titles.add(t);
Display3D.addMesh(common_ls, chain.vs, t, null != c ? c : Color.gray);
}
} else {
for (final Chain chain : bc) Display3D.addMesh(common_ls, chain.vs, chain.getCellTitle(), Color.gray);
}
}
if (show_condensed_3D) {
Display3D.addMesh(common_ls, vs_merged, name + "-condensed", heat_map ? heat_table.get(name) : Color.red);
}
if (show_envelope_3D) {
double[] widths = makeEnvelope(cp, vs_merged);
if (cp.delta_envelope > 1) {
vs_merged.addDependent(widths);
vs_merged.resample(cp.delta_envelope);
widths = vs_merged.getDependent(0);
}
Display3D.addMesh(common_ls, vs_merged, name + "-envelope", heat_map ? heat_table.get(name) : Color.red, widths, env_alpha);
} else if (heat_map) {
// Show spheres in place of envelopes, at the starting tip (neuropile entry point)
final double x = vs_merged.getPoints(0)[0];
final double y = vs_merged.getPoints(1)[0];
final double z = vs_merged.getPoints(2)[0];
final double r = 10;
final Color color = heat_table.get(name);
if (null == color) {
Utils.logAll("WARNING: heat table does not have a color for " + name);
continue;
}
final Content sphere = d3d.getUniverse().addMesh(ij3d.Mesh_Maker.createSphere(x, y, z, r), new Color3f(heat_table.get(name)), name + "-sphere", 1);
}
}
if (show_axes_3D) {
for (int i = 0; i < p.length; i++) {
final Map<String, VectorString3D> m = axes.get(p[i]);
if (null == m) {
Utils.log2("No axes found for project " + p[i]);
continue;
}
for (final Map.Entry<String, VectorString3D> e : m.entrySet()) {
Display3D.addMesh(common_ls, e.getValue(), e.getKey() + "-" + i, Color.gray);
}
}
}
results.put("d3d", Display3D.get(common_ls));
}
this.result = results;
Utils.log2("Done.");
} catch (final Exception e) {
IJError.print(e);
} finally {
finishedWorking();
}
}
};
return Bureaucrat.createAndStart(worker, p[0]);
}
use of ini.trakem2.vector.VectorString3D in project TrakEM2 by trakem2.
the class Compare method transferVectorStrings.
/**
* Transform all points of all VectorString3D in vs using a Moving Least Squares Transform defined by the pairing of points in source to those in target.
* In short, bring source into target.
*/
public static List<VectorString3D> transferVectorStrings(final List<VectorString3D> vs, final List<Tuple3d> source, final List<Tuple3d> target, final Class<AffineModel3D> model_class) throws Exception {
if (source.size() != target.size()) {
Utils.log2("Could not generate a MovingLeastSquaresTransform: different number of source and target points.");
return null;
}
if (source.size() < 1 || target.size() < 1) {
Utils.log2("Cannot transform with less than one point correspondence!");
return null;
}
// 1 - Create the MovingLeastSquaresTransform from the point matches
final ArrayList<PointMatch> pm = new ArrayList<PointMatch>();
for (final Iterator<Tuple3d> si = source.iterator(), ti = target.iterator(); si.hasNext(); ) {
final Tuple3d sp = si.next();
final Tuple3d tp = ti.next();
pm.add(new PointMatch(new mpicbg.models.Point(new double[] { sp.x, sp.y, sp.z }), new mpicbg.models.Point(new double[] { tp.x, tp.y, tp.z }), 1));
}
final MovingLeastSquaresTransform mls = new MovingLeastSquaresTransform();
mls.setModel(model_class);
mls.setMatches(pm);
final double[] point = new double[3];
// 1.1 - Test: transfer source points
/*
for (final Iterator<Tuple3d> si = source.iterator(), ti = target.iterator(); si.hasNext(); ) {
Tuple3d sp = si.next();
point[0] = (double) sp.x;
point[1] = (double) sp.y;
point[2] = (double) sp.z;
mls.applyInPlace(point);
Tuple3d tp = ti.next();
Utils.log2("== target: " + (double)tp.x + ", " + (double)tp.y + ", " + (double)tp.z +
"\n o source: " + (double)sp.x + ", " + (double)sp.y + ", " + (double)sp.z +
"\n source: " + point[0] + ", " + point[1] + ", " + point[2]);
}
*/
// 2 - Transfer each VectorString3D in vs with mls
final List<VectorString3D> vt = new ArrayList<VectorString3D>();
for (final VectorString3D vi : vs) {
// The points of the VectorString3D:
final double[] x = vi.getPoints(0);
final double[] y = vi.getPoints(1);
final double[] z = vi.getPoints(2);
// Empty arrays to fill with the points to transfer:
final double[] tx = new double[x.length];
final double[] ty = new double[x.length];
final double[] tz = new double[x.length];
// Transfer point by point:
for (int i = 0; i < x.length; i++) {
point[0] = x[i];
point[1] = y[i];
point[2] = z[i];
mls.applyInPlace(point);
tx[i] = point[0];
ty[i] = point[1];
tz[i] = point[2];
}
try {
vt.add(new VectorString3D(tx, ty, tz, vi.isClosed()));
} catch (final Exception e) {
}
}
return vt;
}
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