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Example 1 with VectorString3D

use of ini.trakem2.vector.VectorString3D in project TrakEM2 by trakem2.

the class Pipe method measure.

/*
	// debug ---------------
	static private void showShape(final Shape shape) {
		Area area = new Area(shape);
		Rectangle b = area.getBounds();
		AffineTransform trans = new AffineTransform();
		trans.translate(-b.x, -b.y);
		area = area.createTransformedArea(trans);
		ij.process.ByteProcessor bp = new ij.process.ByteProcessor(b.width, b.height);
		ij.gui.ShapeRoi sr = new ij.gui.ShapeRoi(area);
		ij.ImagePlus imp = new ij.ImagePlus("pipe area", bp);
		imp.setRoi(sr);
		bp.setValue(255);
		sr.drawPixels(bp);
		imp.show();
	}
	*/
@Override
public ResultsTable measure(ResultsTable rt) {
    // reload
    if (-1 == n_points)
        setupForDisplay();
    if (0 == n_points)
        return rt;
    if (null == rt)
        rt = Utils.createResultsTable("Pipe results", new String[] { "id", "length", "name-id" });
    // measure length
    double len = 0;
    final Calibration cal = layer_set.getCalibration();
    if (n_points > 1) {
        final VectorString3D vs = asVectorString3D();
        vs.calibrate(cal);
        // no resampling
        len = vs.computeLength();
    }
    rt.incrementCounter();
    rt.addLabel("units", cal.getUnit());
    rt.addValue(0, this.id);
    rt.addValue(1, len);
    rt.addValue(2, getNameId());
    return rt;
}
Also used : VectorString3D(ini.trakem2.vector.VectorString3D) Calibration(ij.measure.Calibration)

Example 2 with VectorString3D

use of ini.trakem2.vector.VectorString3D in project TrakEM2 by trakem2.

the class Pipe method asVectorString3D.

/**
 * Returns a non-calibrated VectorString3D.
 */
@Override
public synchronized VectorString3D asVectorString3D() {
    // local pointers, since they may be transformed
    int n_points = this.n_points;
    double[][] p = this.p;
    // double[][] p_r = this.p_r;
    // double[][] p_l = this.p_l;
    double[][] p_i = this.p_i;
    // double[] p_width = this.p_width;
    double[] p_width_i = this.p_width_i;
    if (!this.at.isIdentity()) {
        final Object[] ob = getTransformedData();
        p = (double[][]) ob[0];
        n_points = p[0].length;
        // p_l = (double[][])ob[1];
        // p_r = (double[][])ob[2];
        p_i = (double[][]) ob[3];
        // p_width = (double[])ob[4];
        p_width_i = (double[]) ob[5];
    }
    final int n = p_i[0].length;
    final int mm = n_points;
    final double[] z_values = new double[n];
    final int interval_points = n / (mm - 1);
    double z_val = 0;
    double z_val_next = 0;
    double z_diff = 0;
    int c = 0;
    double delta = 0;
    for (int j = 0; j < mm - 1; j++) {
        z_val = layer_set.getLayer(p_layer[j]).getZ();
        z_val_next = layer_set.getLayer(p_layer[j + 1]).getZ();
        z_diff = z_val_next - z_val;
        delta = z_diff / interval_points;
        z_values[c] = (0 == j ? z_val : z_values[c - 1]) + delta;
        for (int k = 1; k < interval_points; k++) {
            c++;
            z_values[c] = z_values[c - 1] + delta;
        }
        c++;
    }
    // setting last point
    z_values[n - 1] = layer_set.getLayer(p_layer[mm - 1]).getZ();
    final double[] px = p_i[0];
    final double[] py = p_i[1];
    final double[] pz = z_values;
    VectorString3D vs = null;
    try {
        vs = new VectorString3D(px, py, pz, false);
        vs.addDependent(p_width_i);
    } catch (final Exception e) {
        IJError.print(e);
    }
    return vs;
}
Also used : VectorString3D(ini.trakem2.vector.VectorString3D)

Example 3 with VectorString3D

use of ini.trakem2.vector.VectorString3D in project TrakEM2 by trakem2.

the class Polyline method asVectorString3D.

/**
 * Returns a non-calibrated VectorString3D.
 */
@Override
public synchronized VectorString3D asVectorString3D() {
    // local pointers, since they may be transformed
    int n_points = this.n_points;
    double[][] p = this.p;
    if (!this.at.isIdentity()) {
        final Object[] ob = getTransformedData();
        p = (double[][]) ob[0];
        n_points = p[0].length;
    }
    final double[] z_values = new double[n_points];
    for (int i = 0; i < n_points; i++) {
        z_values[i] = layer_set.getLayer(p_layer[i]).getZ();
    }
    final double[] px = p[0];
    final double[] py = p[1];
    final double[] pz = z_values;
    VectorString3D vs = null;
    try {
        vs = new VectorString3D(px, py, pz, false);
    } catch (final Exception e) {
        IJError.print(e);
    }
    return vs;
}
Also used : VectorString3D(ini.trakem2.vector.VectorString3D) NoninvertibleTransformException(java.awt.geom.NoninvertibleTransformException)

Example 4 with VectorString3D

use of ini.trakem2.vector.VectorString3D in project TrakEM2 by trakem2.

the class Compare method variabilityAnalysis.

/**
 * @param reference_project If null, then the first one found in the Project.getProjects() lists is used.
 * @param regex A String (can be null) to filter objects by, to limit what gets processed.
 *              If regex is not null, then only ProjectThing nodes with the matching regex are analyzed (shallow: none of their children are questioned, but pipes will be built from them all).
 * @param generate_plots Whether to generate the variability plots at all.
 * @param show_plots If generate_plots, whether to show the plots in a stack image window or to save them.
 * @param show_3D Whether to show any 3D data.
 * @param show_condensed_3D If show_3D, whether to show the condensed vector strings, i.e. the "average" pipes.
 * @param show_sources_3D If show_3D, whether to show the source pipes from which the condensed vector string was generated.
 * @param sources_color_table Which colors to give to the pipes of which Project.
 * @param show_envelope_3D If show_3D, whether to generate the variability envelope.
 * @param envelope_alpha If show_envelope_3D, the envelope takes an alpha value between 0 (total transparency) and 1 (total opacity)
 * @param delta_envelope The delta to resample the envelope to. When smaller than or equal to 1, no envelope resampling occurs.
 * @param show_axes_3D If show_3D, whether to display the reference axes as well.
 * @param heat_map If show_3D, whether to color the variability with a Fire LUT.
 *                 If not show_condensed_3D, then the variability is shown in color-coded 3D spheres placed at the entry point to the neuropile.
 * @param map_condensed If not null, all VectorString3D are put into this map.
 * @param projects The projects to use.
 */
public static Bureaucrat variabilityAnalysis(final Project reference_project, final String regex, final String[] ignore, final boolean show_cata_dialog, final boolean generate_plots, final boolean show_plots, final String plot_dir_, final boolean show_3D, final boolean show_condensed_3D, final boolean show_sources_3D, final Map<Project, Color> sources_color_table, final boolean show_envelope_3D, final float envelope_alpha, final double delta_envelope, final int envelope_type, final boolean show_axes_3D, final boolean heat_map, final Map<String, VectorString3D> map_condensed, final Project[] projects) {
    // gather all open projects
    final Project[] p = null == projects ? Project.getProjects().toArray(new Project[0]) : projects;
    // make the reference_project be the first in the array
    if (null != reference_project && reference_project != p[0]) {
        for (int i = 0; i < p.length; i++) {
            if (reference_project == p[i]) {
                p[i] = p[0];
                p[0] = reference_project;
                break;
            }
        }
    }
    final Worker worker = new Worker("Comparing all to all") {

        @Override
        public void run() {
            startedWorking();
            try {
                Utils.log2("Asking for CATAParameters...");
                final CATAParameters cp = new CATAParameters();
                cp.regex = regex;
                cp.delta_envelope = delta_envelope;
                cp.envelope_type = envelope_type;
                if (show_cata_dialog && !cp.setup(false, regex, true, true)) {
                    finishedWorking();
                    return;
                }
                // so source points are stored in VectorString3D for each resampled and interpolated point
                cp.with_source = true;
                // Store a series of results, depending on options
                final HashMap<String, Display3D> results = new HashMap<String, Display3D>();
                String plot_dir = plot_dir_;
                if (generate_plots && !show_plots) {
                    // Save plots
                    if (null == plot_dir) {
                        final DirectoryChooser dc = new DirectoryChooser("Choose plots directory");
                        plot_dir = dc.getDirectory();
                        if (null == plot_dir) {
                            finishedWorking();
                            return;
                        }
                    }
                    if (IJ.isWindows())
                        plot_dir = plot_dir.replace('\\', '/');
                    if (!plot_dir.endsWith("/"))
                        plot_dir += "/";
                }
                Utils.log2("Gathering chains...");
                // Gather chains that do not match the ignore regexes
                // will transform them as well to the reference found in the first project in the p array
                Object[] ob = gatherChains(p, cp, ignore);
                ArrayList<Chain> chains = (ArrayList<Chain>) ob[0];
                // to keep track of each project's chains
                final ArrayList[] p_chains = (ArrayList[]) ob[1];
                ob = null;
                if (null == chains) {
                    finishedWorking();
                    return;
                }
                Utils.log2("Collecting bundles...");
                final HashMap<Project, HashMap<String, VectorString3D>> axes = new HashMap<Project, HashMap<String, VectorString3D>>();
                // Sort out into groups by unique names of lineage bundles
                final HashMap<String, ArrayList<Chain>> bundles = new HashMap<String, ArrayList<Chain>>();
                for (final Chain chain : chains) {
                    String title = chain.getCellTitle();
                    final String t = title.toLowerCase();
                    // unnamed
                    if (0 == t.indexOf('[') || 0 == t.indexOf('#'))
                        continue;
                    Utils.log("Accepting " + title);
                    title = title.substring(0, title.indexOf(' '));
                    // lineage bundle instance chains
                    ArrayList<Chain> bc = bundles.get(title);
                    if (null == bc) {
                        bc = new ArrayList<Chain>();
                        bundles.put(title, bc);
                    }
                    bc.add(chain);
                }
                Utils.log2("Found " + bundles.size() + " bundles.");
                chains = null;
                if (null != cp.regex && show_axes_3D && axes.size() < 3) {
                    // Must find the Mushroom Body lobes separately
                    final String cp_regex = cp.regex;
                    cp.regex = "mb";
                    final Object[] o = gatherChains(p, cp, ignore);
                    final ArrayList<Chain> lobes = (ArrayList<Chain>) o[0];
                    Utils.logAll("Found " + lobes.size() + " chains for lobes");
                    for (final Chain chain : lobes) {
                        final String t = chain.getCellTitle().toLowerCase();
                        if (-1 != t.indexOf("peduncle") || -1 != t.indexOf("medial lobe") || -1 != t.indexOf("dorsal lobe")) {
                            Utils.logAll("adding " + t);
                            final Project pr = chain.pipes.get(0).getProject();
                            HashMap<String, VectorString3D> m = axes.get(pr);
                            if (null == m) {
                                m = new HashMap<String, VectorString3D>();
                                axes.put(pr, m);
                            }
                            m.put(t, chain.vs);
                            continue;
                        }
                    }
                    cp.regex = cp_regex;
                } else {
                    Utils.logAll("Not: cp.regex = " + cp.regex + "  show_axes_3D = " + show_axes_3D + "  axes.size() = " + axes.size());
                }
                final HashMap<String, VectorString3D> condensed = new HashMap<String, VectorString3D>();
                Utils.log2("Condensing each bundle...");
                // Condense each into a single VectorString3D
                for (final Map.Entry<String, ArrayList<Chain>> entry : bundles.entrySet()) {
                    final ArrayList<Chain> bc = entry.getValue();
                    if (bc.size() < 2) {
                        Utils.log2("Skipping single: " + entry.getKey());
                        continue;
                    }
                    final VectorString3D[] vs = new VectorString3D[bc.size()];
                    for (int i = 0; i < vs.length; i++) vs[i] = bc.get(i).vs;
                    final VectorString3D c = condense(cp, vs, this);
                    c.setCalibration(p[0].getRootLayerSet().getCalibrationCopy());
                    condensed.put(entry.getKey(), c);
                    if (this.hasQuitted())
                        return;
                }
                // Store:
                if (null != map_condensed) {
                    map_condensed.putAll(condensed);
                }
                if (generate_plots) {
                    Utils.log2("Plotting stdDev for each condensed bundle...");
                    // Y axis: the stdDev at each point, computed from the group of points that contribute to each
                    for (final Map.Entry<String, VectorString3D> e : condensed.entrySet()) {
                        final String name = e.getKey();
                        final VectorString3D c = e.getValue();
                        final Plot plot = makePlot(cp, name, c);
                        // FAILS//plot.addLabel(10, cp.plot_height-5, name); // must be added after setting size
                        if (show_plots)
                            plot.show();
                        else if (null != plot_dir)
                            new FileSaver(plot.getImagePlus()).saveAsPng(plot_dir + name.replace('/', '-') + ".png");
                    }
                }
                if (show_3D) {
                    final HashMap<String, Color> heat_table = new HashMap<String, Color>();
                    if (heat_map || show_envelope_3D) {
                        // Create a Fire LUT
                        final ImagePlus lutimp = new ImagePlus("lut", new ByteProcessor(4, 4));
                        IJ.run(lutimp, "Fire", "");
                        final IndexColorModel icm = (IndexColorModel) lutimp.getProcessor().getColorModel();
                        final byte[] reds = new byte[256];
                        final byte[] greens = new byte[256];
                        final byte[] blues = new byte[256];
                        icm.getReds(reds);
                        icm.getGreens(greens);
                        icm.getBlues(blues);
                        final List<String> names = new ArrayList<String>(bundles.keySet());
                        Collections.sort(names);
                        // find max stdDev
                        double max = 0;
                        final HashMap<String, Double> heats = new HashMap<String, Double>();
                        for (final String name : names) {
                            final VectorString3D vs_merged = condensed.get(name);
                            if (null == vs_merged) {
                                Utils.logAll("WARNING could not find a condensed pipe for " + name);
                                continue;
                            }
                            final double[] stdDev = vs_merged.getStdDevAtEachPoint();
                            // double avg = 0;
                            // for (int i=0; i<stdDev.length; i++) avg += stdDev[i];
                            // avg = avg/stdDev.length;
                            Arrays.sort(stdDev);
                            // median is more representative than average
                            final double median = stdDev[stdDev.length / 2];
                            if (max < median)
                                max = median;
                            heats.put(name, median);
                        }
                        for (final Map.Entry<String, Double> e : heats.entrySet()) {
                            final String name = e.getKey();
                            final double median = e.getValue();
                            // scale between 0 and max to get a Fire LUT color:
                            int index = (int) ((median / max) * 255);
                            if (index > 255)
                                index = 255;
                            final Color color = new Color(0xff & reds[index], 0xff & greens[index], 0xff & blues[index]);
                            Utils.log2(new StringBuilder(name).append('\t').append(median).append('\t').append(reds[index]).append('\t').append(greens[index]).append('\t').append(blues[index]).toString());
                            heat_table.put(name, color);
                        }
                    }
                    final LayerSet common_ls = new LayerSet(p[0], -1, "Common", 10, 10, 0, 0, 0, 512, 512, false, 2, new AffineTransform());
                    final Display3D d3d = Display3D.get(common_ls);
                    float env_alpha = envelope_alpha;
                    if (env_alpha < 0) {
                        Utils.log2("WARNING envelope_alpha is invalid: " + envelope_alpha + "\n    Using 0.4f instead");
                        env_alpha = 0.4f;
                    } else if (env_alpha > 1)
                        env_alpha = 1.0f;
                    for (final String name : bundles.keySet()) {
                        final ArrayList<Chain> bc = bundles.get(name);
                        final VectorString3D vs_merged = condensed.get(name);
                        if (null == vs_merged) {
                            Utils.logAll("WARNING: could not find a condensed vs for " + name);
                            continue;
                        }
                        if (show_sources_3D) {
                            if (null != sources_color_table) {
                                final HashSet<String> titles = new HashSet<String>();
                                for (final Chain chain : bc) {
                                    final Color c = sources_color_table.get(chain.getRoot().getProject());
                                    final String title = chain.getCellTitle();
                                    String t = title;
                                    int i = 2;
                                    while (titles.contains(t)) {
                                        t = title + "-" + i;
                                        i += 1;
                                    }
                                    titles.add(t);
                                    Display3D.addMesh(common_ls, chain.vs, t, null != c ? c : Color.gray);
                                }
                            } else {
                                for (final Chain chain : bc) Display3D.addMesh(common_ls, chain.vs, chain.getCellTitle(), Color.gray);
                            }
                        }
                        if (show_condensed_3D) {
                            Display3D.addMesh(common_ls, vs_merged, name + "-condensed", heat_map ? heat_table.get(name) : Color.red);
                        }
                        if (show_envelope_3D) {
                            double[] widths = makeEnvelope(cp, vs_merged);
                            if (cp.delta_envelope > 1) {
                                vs_merged.addDependent(widths);
                                vs_merged.resample(cp.delta_envelope);
                                widths = vs_merged.getDependent(0);
                            }
                            Display3D.addMesh(common_ls, vs_merged, name + "-envelope", heat_map ? heat_table.get(name) : Color.red, widths, env_alpha);
                        } else if (heat_map) {
                            // Show spheres in place of envelopes, at the starting tip (neuropile entry point)
                            final double x = vs_merged.getPoints(0)[0];
                            final double y = vs_merged.getPoints(1)[0];
                            final double z = vs_merged.getPoints(2)[0];
                            final double r = 10;
                            final Color color = heat_table.get(name);
                            if (null == color) {
                                Utils.logAll("WARNING: heat table does not have a color for " + name);
                                continue;
                            }
                            final Content sphere = d3d.getUniverse().addMesh(ij3d.Mesh_Maker.createSphere(x, y, z, r), new Color3f(heat_table.get(name)), name + "-sphere", 1);
                        }
                    }
                    if (show_axes_3D) {
                        for (int i = 0; i < p.length; i++) {
                            final Map<String, VectorString3D> m = axes.get(p[i]);
                            if (null == m) {
                                Utils.log2("No axes found for project " + p[i]);
                                continue;
                            }
                            for (final Map.Entry<String, VectorString3D> e : m.entrySet()) {
                                Display3D.addMesh(common_ls, e.getValue(), e.getKey() + "-" + i, Color.gray);
                            }
                        }
                    }
                    results.put("d3d", Display3D.get(common_ls));
                }
                this.result = results;
                Utils.log2("Done.");
            } catch (final Exception e) {
                IJError.print(e);
            } finally {
                finishedWorking();
            }
        }
    };
    return Bureaucrat.createAndStart(worker, p[0]);
}
Also used : CATAParameters(ini.trakem2.analysis.Compare.CATAParameters) ByteProcessor(ij.process.ByteProcessor) HashMap(java.util.HashMap) Color3f(org.scijava.vecmath.Color3f) ArrayList(java.util.ArrayList) FileSaver(ij.io.FileSaver) Worker(ini.trakem2.utils.Worker) IndexColorModel(java.awt.image.IndexColorModel) HashSet(java.util.HashSet) LayerSet(ini.trakem2.display.LayerSet) Display3D(ini.trakem2.display.Display3D) Plot(ij.gui.Plot) Color(java.awt.Color) ImagePlus(ij.ImagePlus) Project(ini.trakem2.Project) VectorString3D(ini.trakem2.vector.VectorString3D) Content(ij3d.Content) AffineTransform(java.awt.geom.AffineTransform) Map(java.util.Map) HashMap(java.util.HashMap) TreeMap(java.util.TreeMap) DirectoryChooser(ij.io.DirectoryChooser)

Example 5 with VectorString3D

use of ini.trakem2.vector.VectorString3D in project TrakEM2 by trakem2.

the class Compare method transferVectorStrings.

/**
 * Transform all points of all VectorString3D in vs using a Moving Least Squares Transform defined by the pairing of points in source to those in target.
 *  In short, bring source into target.
 */
public static List<VectorString3D> transferVectorStrings(final List<VectorString3D> vs, final List<Tuple3d> source, final List<Tuple3d> target, final Class<AffineModel3D> model_class) throws Exception {
    if (source.size() != target.size()) {
        Utils.log2("Could not generate a MovingLeastSquaresTransform: different number of source and target points.");
        return null;
    }
    if (source.size() < 1 || target.size() < 1) {
        Utils.log2("Cannot transform with less than one point correspondence!");
        return null;
    }
    // 1 - Create the MovingLeastSquaresTransform from the point matches
    final ArrayList<PointMatch> pm = new ArrayList<PointMatch>();
    for (final Iterator<Tuple3d> si = source.iterator(), ti = target.iterator(); si.hasNext(); ) {
        final Tuple3d sp = si.next();
        final Tuple3d tp = ti.next();
        pm.add(new PointMatch(new mpicbg.models.Point(new double[] { sp.x, sp.y, sp.z }), new mpicbg.models.Point(new double[] { tp.x, tp.y, tp.z }), 1));
    }
    final MovingLeastSquaresTransform mls = new MovingLeastSquaresTransform();
    mls.setModel(model_class);
    mls.setMatches(pm);
    final double[] point = new double[3];
    // 1.1 - Test: transfer source points
    /*
		for (final Iterator<Tuple3d> si = source.iterator(), ti = target.iterator(); si.hasNext(); ) {
			Tuple3d sp = si.next();
			point[0] = (double) sp.x;
			point[1] = (double) sp.y;
			point[2] = (double) sp.z;
			mls.applyInPlace(point);

			Tuple3d tp = ti.next();
			Utils.log2("== target: " + (double)tp.x + ", " + (double)tp.y + ", " + (double)tp.z +
				   "\n o source: " + (double)sp.x + ", " + (double)sp.y + ", " + (double)sp.z +

				   "\n   source: " + point[0] + ", " + point[1] + ", " + point[2]);
		}
		*/
    // 2 - Transfer each VectorString3D in vs with mls
    final List<VectorString3D> vt = new ArrayList<VectorString3D>();
    for (final VectorString3D vi : vs) {
        // The points of the VectorString3D:
        final double[] x = vi.getPoints(0);
        final double[] y = vi.getPoints(1);
        final double[] z = vi.getPoints(2);
        // Empty arrays to fill with the points to transfer:
        final double[] tx = new double[x.length];
        final double[] ty = new double[x.length];
        final double[] tz = new double[x.length];
        // Transfer point by point:
        for (int i = 0; i < x.length; i++) {
            point[0] = x[i];
            point[1] = y[i];
            point[2] = z[i];
            mls.applyInPlace(point);
            tx[i] = point[0];
            ty[i] = point[1];
            tz[i] = point[2];
        }
        try {
            vt.add(new VectorString3D(tx, ty, tz, vi.isClosed()));
        } catch (final Exception e) {
        }
    }
    return vt;
}
Also used : ArrayList(java.util.ArrayList) PointMatch(mpicbg.models.PointMatch) MovingLeastSquaresTransform(mpicbg.models.MovingLeastSquaresTransform) VectorString3D(ini.trakem2.vector.VectorString3D) Tuple3d(org.scijava.vecmath.Tuple3d)

Aggregations

VectorString3D (ini.trakem2.vector.VectorString3D)19 ArrayList (java.util.ArrayList)6 Editions (ini.trakem2.vector.Editions)5 Calibration (ij.measure.Calibration)4 Project (ini.trakem2.Project)4 HashMap (java.util.HashMap)4 Map (java.util.Map)4 TreeMap (java.util.TreeMap)4 CATAParameters (ini.trakem2.analysis.Compare.CATAParameters)3 Worker (ini.trakem2.utils.Worker)3 HashSet (java.util.HashSet)3 AtomicInteger (java.util.concurrent.atomic.AtomicInteger)3 Vector3d (org.scijava.vecmath.Vector3d)3 File (java.io.File)2 Tuple3d (org.scijava.vecmath.Tuple3d)2 ImagePlus (ij.ImagePlus)1 OvalRoi (ij.gui.OvalRoi)1 Plot (ij.gui.Plot)1 DirectoryChooser (ij.io.DirectoryChooser)1 FileSaver (ij.io.FileSaver)1