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Example 1 with Plot

use of ij.gui.Plot in project GDSC-SMLM by aherbert.

the class PSFDrift method showHWHM.

private void showHWHM(List<String> titles) {
    GenericDialog gd = new GenericDialog(TITLE);
    gd.addMessage("Select the input PSF image");
    gd.addChoice("PSF", titles.toArray(new String[titles.size()]), title);
    gd.addCheckbox("Use_offset", useOffset);
    gd.addNumericField("Scale", scale, 2);
    gd.showDialog();
    if (gd.wasCanceled())
        return;
    title = gd.getNextChoice();
    useOffset = gd.getNextBoolean();
    scale = gd.getNextNumber();
    imp = WindowManager.getImage(title);
    if (imp == null) {
        IJ.error(TITLE, "No PSF image for image: " + title);
        return;
    }
    psfSettings = getPSFSettings(imp);
    if (psfSettings == null) {
        IJ.error(TITLE, "No PSF settings for image: " + title);
        return;
    }
    int size = imp.getStackSize();
    ImagePSFModel psf = createImagePSF(1, size);
    double[] w0 = psf.getAllHWHM0();
    double[] w1 = psf.getAllHWHM1();
    double[] slice = Utils.newArray(w0.length, 1, 1.0);
    // Widths are in pixels
    String title = TITLE + " HWHM";
    Plot plot = new Plot(title, "Slice", "HWHM (px)");
    double[] limits = Maths.limits(w0);
    limits = Maths.limits(limits, w1);
    plot.setLimits(1, size, 0, limits[1] * 1.05);
    plot.setColor(Color.red);
    plot.addPoints(slice, w0, Plot.LINE);
    plot.setColor(Color.blue);
    plot.addPoints(slice, w1, Plot.LINE);
    plot.setColor(Color.black);
    plot.addLabel(0, 0, "X=red; Y=blue");
    Utils.display(title, plot);
}
Also used : GenericDialog(ij.gui.GenericDialog) Plot(ij.gui.Plot) ImagePSFModel(gdsc.smlm.model.ImagePSFModel)

Example 2 with Plot

use of ij.gui.Plot in project GDSC-SMLM by aherbert.

the class DoubletAnalysis method plotJaccard.

private void plotJaccard(ResidualsScore residualsScore, ResidualsScore residualsScoreReference) {
    String title = TITLE + " Score";
    Plot plot = new Plot(title, "Residuals", "Score");
    double max = getMax(0.01, residualsScore);
    if (residualsScoreReference != null) {
        max = getMax(max, residualsScore);
    }
    plot.setLimits(0, 1, 0, max * 1.05);
    addLines(plot, residualsScore, 1);
    if (residualsScoreReference != null) {
        addLines(plot, residualsScoreReference, 0.5);
    }
    plot.setColor(Color.black);
    plot.addLabel(0, 0, String.format("Residuals %s; Jaccard %s (Black); Precision %s (Blue); Recall %s (Red)", Utils.rounded(residualsScore.residuals[residualsScore.maxJaccardIndex]), Utils.rounded(residualsScore.jaccard[residualsScore.maxJaccardIndex]), Utils.rounded(residualsScore.precision[residualsScore.maxJaccardIndex]), Utils.rounded(residualsScore.recall[residualsScore.maxJaccardIndex])));
    display(title, plot);
}
Also used : Plot(ij.gui.Plot)

Example 3 with Plot

use of ij.gui.Plot in project GDSC-SMLM by aherbert.

the class CMOSAnalysis method showHistogram.

private void showHistogram(String name, double[] values, int bins, Statistics stats, WindowOrganiser wo) {
    DoubleData data = new StoredData(values, false);
    double minWidth = 0;
    int removeOutliers = 0;
    // Plot2.BAR; // A bar chart confuses the log plot since it plots lines to zero.
    int shape = Plot.CIRCLE;
    String label = String.format("Mean = %s +/- %s", Utils.rounded(stats.getMean()), Utils.rounded(stats.getStandardDeviation()));
    int id = Utils.showHistogram(TITLE, data, name, minWidth, removeOutliers, bins, shape, label);
    if (Utils.isNewWindow())
        wo.add(id);
    // Redraw using a log scale. This requires a non-zero y-min
    Plot plot = Utils.plot;
    double[] limits = plot.getLimits();
    plot.setLimits(limits[0], limits[1], 1, limits[3]);
    plot.setAxisYLog(true);
    Utils.plot.updateImage();
}
Also used : Plot(ij.gui.Plot) StoredData(gdsc.core.utils.StoredData) DoubleData(gdsc.core.utils.DoubleData)

Example 4 with Plot

use of ij.gui.Plot in project TrakEM2 by trakem2.

the class Compare method variabilityAnalysis.

/**
 * @param reference_project If null, then the first one found in the Project.getProjects() lists is used.
 * @param regex A String (can be null) to filter objects by, to limit what gets processed.
 *              If regex is not null, then only ProjectThing nodes with the matching regex are analyzed (shallow: none of their children are questioned, but pipes will be built from them all).
 * @param generate_plots Whether to generate the variability plots at all.
 * @param show_plots If generate_plots, whether to show the plots in a stack image window or to save them.
 * @param show_3D Whether to show any 3D data.
 * @param show_condensed_3D If show_3D, whether to show the condensed vector strings, i.e. the "average" pipes.
 * @param show_sources_3D If show_3D, whether to show the source pipes from which the condensed vector string was generated.
 * @param sources_color_table Which colors to give to the pipes of which Project.
 * @param show_envelope_3D If show_3D, whether to generate the variability envelope.
 * @param envelope_alpha If show_envelope_3D, the envelope takes an alpha value between 0 (total transparency) and 1 (total opacity)
 * @param delta_envelope The delta to resample the envelope to. When smaller than or equal to 1, no envelope resampling occurs.
 * @param show_axes_3D If show_3D, whether to display the reference axes as well.
 * @param heat_map If show_3D, whether to color the variability with a Fire LUT.
 *                 If not show_condensed_3D, then the variability is shown in color-coded 3D spheres placed at the entry point to the neuropile.
 * @param map_condensed If not null, all VectorString3D are put into this map.
 * @param projects The projects to use.
 */
public static Bureaucrat variabilityAnalysis(final Project reference_project, final String regex, final String[] ignore, final boolean show_cata_dialog, final boolean generate_plots, final boolean show_plots, final String plot_dir_, final boolean show_3D, final boolean show_condensed_3D, final boolean show_sources_3D, final Map<Project, Color> sources_color_table, final boolean show_envelope_3D, final float envelope_alpha, final double delta_envelope, final int envelope_type, final boolean show_axes_3D, final boolean heat_map, final Map<String, VectorString3D> map_condensed, final Project[] projects) {
    // gather all open projects
    final Project[] p = null == projects ? Project.getProjects().toArray(new Project[0]) : projects;
    // make the reference_project be the first in the array
    if (null != reference_project && reference_project != p[0]) {
        for (int i = 0; i < p.length; i++) {
            if (reference_project == p[i]) {
                p[i] = p[0];
                p[0] = reference_project;
                break;
            }
        }
    }
    final Worker worker = new Worker("Comparing all to all") {

        @Override
        public void run() {
            startedWorking();
            try {
                Utils.log2("Asking for CATAParameters...");
                final CATAParameters cp = new CATAParameters();
                cp.regex = regex;
                cp.delta_envelope = delta_envelope;
                cp.envelope_type = envelope_type;
                if (show_cata_dialog && !cp.setup(false, regex, true, true)) {
                    finishedWorking();
                    return;
                }
                // so source points are stored in VectorString3D for each resampled and interpolated point
                cp.with_source = true;
                // Store a series of results, depending on options
                final HashMap<String, Display3D> results = new HashMap<String, Display3D>();
                String plot_dir = plot_dir_;
                if (generate_plots && !show_plots) {
                    // Save plots
                    if (null == plot_dir) {
                        final DirectoryChooser dc = new DirectoryChooser("Choose plots directory");
                        plot_dir = dc.getDirectory();
                        if (null == plot_dir) {
                            finishedWorking();
                            return;
                        }
                    }
                    if (IJ.isWindows())
                        plot_dir = plot_dir.replace('\\', '/');
                    if (!plot_dir.endsWith("/"))
                        plot_dir += "/";
                }
                Utils.log2("Gathering chains...");
                // Gather chains that do not match the ignore regexes
                // will transform them as well to the reference found in the first project in the p array
                Object[] ob = gatherChains(p, cp, ignore);
                ArrayList<Chain> chains = (ArrayList<Chain>) ob[0];
                // to keep track of each project's chains
                final ArrayList[] p_chains = (ArrayList[]) ob[1];
                ob = null;
                if (null == chains) {
                    finishedWorking();
                    return;
                }
                Utils.log2("Collecting bundles...");
                final HashMap<Project, HashMap<String, VectorString3D>> axes = new HashMap<Project, HashMap<String, VectorString3D>>();
                // Sort out into groups by unique names of lineage bundles
                final HashMap<String, ArrayList<Chain>> bundles = new HashMap<String, ArrayList<Chain>>();
                for (final Chain chain : chains) {
                    String title = chain.getCellTitle();
                    final String t = title.toLowerCase();
                    // unnamed
                    if (0 == t.indexOf('[') || 0 == t.indexOf('#'))
                        continue;
                    Utils.log("Accepting " + title);
                    title = title.substring(0, title.indexOf(' '));
                    // lineage bundle instance chains
                    ArrayList<Chain> bc = bundles.get(title);
                    if (null == bc) {
                        bc = new ArrayList<Chain>();
                        bundles.put(title, bc);
                    }
                    bc.add(chain);
                }
                Utils.log2("Found " + bundles.size() + " bundles.");
                chains = null;
                if (null != cp.regex && show_axes_3D && axes.size() < 3) {
                    // Must find the Mushroom Body lobes separately
                    final String cp_regex = cp.regex;
                    cp.regex = "mb";
                    final Object[] o = gatherChains(p, cp, ignore);
                    final ArrayList<Chain> lobes = (ArrayList<Chain>) o[0];
                    Utils.logAll("Found " + lobes.size() + " chains for lobes");
                    for (final Chain chain : lobes) {
                        final String t = chain.getCellTitle().toLowerCase();
                        if (-1 != t.indexOf("peduncle") || -1 != t.indexOf("medial lobe") || -1 != t.indexOf("dorsal lobe")) {
                            Utils.logAll("adding " + t);
                            final Project pr = chain.pipes.get(0).getProject();
                            HashMap<String, VectorString3D> m = axes.get(pr);
                            if (null == m) {
                                m = new HashMap<String, VectorString3D>();
                                axes.put(pr, m);
                            }
                            m.put(t, chain.vs);
                            continue;
                        }
                    }
                    cp.regex = cp_regex;
                } else {
                    Utils.logAll("Not: cp.regex = " + cp.regex + "  show_axes_3D = " + show_axes_3D + "  axes.size() = " + axes.size());
                }
                final HashMap<String, VectorString3D> condensed = new HashMap<String, VectorString3D>();
                Utils.log2("Condensing each bundle...");
                // Condense each into a single VectorString3D
                for (final Map.Entry<String, ArrayList<Chain>> entry : bundles.entrySet()) {
                    final ArrayList<Chain> bc = entry.getValue();
                    if (bc.size() < 2) {
                        Utils.log2("Skipping single: " + entry.getKey());
                        continue;
                    }
                    final VectorString3D[] vs = new VectorString3D[bc.size()];
                    for (int i = 0; i < vs.length; i++) vs[i] = bc.get(i).vs;
                    final VectorString3D c = condense(cp, vs, this);
                    c.setCalibration(p[0].getRootLayerSet().getCalibrationCopy());
                    condensed.put(entry.getKey(), c);
                    if (this.hasQuitted())
                        return;
                }
                // Store:
                if (null != map_condensed) {
                    map_condensed.putAll(condensed);
                }
                if (generate_plots) {
                    Utils.log2("Plotting stdDev for each condensed bundle...");
                    // Y axis: the stdDev at each point, computed from the group of points that contribute to each
                    for (final Map.Entry<String, VectorString3D> e : condensed.entrySet()) {
                        final String name = e.getKey();
                        final VectorString3D c = e.getValue();
                        final Plot plot = makePlot(cp, name, c);
                        // FAILS//plot.addLabel(10, cp.plot_height-5, name); // must be added after setting size
                        if (show_plots)
                            plot.show();
                        else if (null != plot_dir)
                            new FileSaver(plot.getImagePlus()).saveAsPng(plot_dir + name.replace('/', '-') + ".png");
                    }
                }
                if (show_3D) {
                    final HashMap<String, Color> heat_table = new HashMap<String, Color>();
                    if (heat_map || show_envelope_3D) {
                        // Create a Fire LUT
                        final ImagePlus lutimp = new ImagePlus("lut", new ByteProcessor(4, 4));
                        IJ.run(lutimp, "Fire", "");
                        final IndexColorModel icm = (IndexColorModel) lutimp.getProcessor().getColorModel();
                        final byte[] reds = new byte[256];
                        final byte[] greens = new byte[256];
                        final byte[] blues = new byte[256];
                        icm.getReds(reds);
                        icm.getGreens(greens);
                        icm.getBlues(blues);
                        final List<String> names = new ArrayList<String>(bundles.keySet());
                        Collections.sort(names);
                        // find max stdDev
                        double max = 0;
                        final HashMap<String, Double> heats = new HashMap<String, Double>();
                        for (final String name : names) {
                            final VectorString3D vs_merged = condensed.get(name);
                            if (null == vs_merged) {
                                Utils.logAll("WARNING could not find a condensed pipe for " + name);
                                continue;
                            }
                            final double[] stdDev = vs_merged.getStdDevAtEachPoint();
                            // double avg = 0;
                            // for (int i=0; i<stdDev.length; i++) avg += stdDev[i];
                            // avg = avg/stdDev.length;
                            Arrays.sort(stdDev);
                            // median is more representative than average
                            final double median = stdDev[stdDev.length / 2];
                            if (max < median)
                                max = median;
                            heats.put(name, median);
                        }
                        for (final Map.Entry<String, Double> e : heats.entrySet()) {
                            final String name = e.getKey();
                            final double median = e.getValue();
                            // scale between 0 and max to get a Fire LUT color:
                            int index = (int) ((median / max) * 255);
                            if (index > 255)
                                index = 255;
                            final Color color = new Color(0xff & reds[index], 0xff & greens[index], 0xff & blues[index]);
                            Utils.log2(new StringBuilder(name).append('\t').append(median).append('\t').append(reds[index]).append('\t').append(greens[index]).append('\t').append(blues[index]).toString());
                            heat_table.put(name, color);
                        }
                    }
                    final LayerSet common_ls = new LayerSet(p[0], -1, "Common", 10, 10, 0, 0, 0, 512, 512, false, 2, new AffineTransform());
                    final Display3D d3d = Display3D.get(common_ls);
                    float env_alpha = envelope_alpha;
                    if (env_alpha < 0) {
                        Utils.log2("WARNING envelope_alpha is invalid: " + envelope_alpha + "\n    Using 0.4f instead");
                        env_alpha = 0.4f;
                    } else if (env_alpha > 1)
                        env_alpha = 1.0f;
                    for (final String name : bundles.keySet()) {
                        final ArrayList<Chain> bc = bundles.get(name);
                        final VectorString3D vs_merged = condensed.get(name);
                        if (null == vs_merged) {
                            Utils.logAll("WARNING: could not find a condensed vs for " + name);
                            continue;
                        }
                        if (show_sources_3D) {
                            if (null != sources_color_table) {
                                final HashSet<String> titles = new HashSet<String>();
                                for (final Chain chain : bc) {
                                    final Color c = sources_color_table.get(chain.getRoot().getProject());
                                    final String title = chain.getCellTitle();
                                    String t = title;
                                    int i = 2;
                                    while (titles.contains(t)) {
                                        t = title + "-" + i;
                                        i += 1;
                                    }
                                    titles.add(t);
                                    Display3D.addMesh(common_ls, chain.vs, t, null != c ? c : Color.gray);
                                }
                            } else {
                                for (final Chain chain : bc) Display3D.addMesh(common_ls, chain.vs, chain.getCellTitle(), Color.gray);
                            }
                        }
                        if (show_condensed_3D) {
                            Display3D.addMesh(common_ls, vs_merged, name + "-condensed", heat_map ? heat_table.get(name) : Color.red);
                        }
                        if (show_envelope_3D) {
                            double[] widths = makeEnvelope(cp, vs_merged);
                            if (cp.delta_envelope > 1) {
                                vs_merged.addDependent(widths);
                                vs_merged.resample(cp.delta_envelope);
                                widths = vs_merged.getDependent(0);
                            }
                            Display3D.addMesh(common_ls, vs_merged, name + "-envelope", heat_map ? heat_table.get(name) : Color.red, widths, env_alpha);
                        } else if (heat_map) {
                            // Show spheres in place of envelopes, at the starting tip (neuropile entry point)
                            final double x = vs_merged.getPoints(0)[0];
                            final double y = vs_merged.getPoints(1)[0];
                            final double z = vs_merged.getPoints(2)[0];
                            final double r = 10;
                            final Color color = heat_table.get(name);
                            if (null == color) {
                                Utils.logAll("WARNING: heat table does not have a color for " + name);
                                continue;
                            }
                            final Content sphere = d3d.getUniverse().addMesh(ij3d.Mesh_Maker.createSphere(x, y, z, r), new Color3f(heat_table.get(name)), name + "-sphere", 1);
                        }
                    }
                    if (show_axes_3D) {
                        for (int i = 0; i < p.length; i++) {
                            final Map<String, VectorString3D> m = axes.get(p[i]);
                            if (null == m) {
                                Utils.log2("No axes found for project " + p[i]);
                                continue;
                            }
                            for (final Map.Entry<String, VectorString3D> e : m.entrySet()) {
                                Display3D.addMesh(common_ls, e.getValue(), e.getKey() + "-" + i, Color.gray);
                            }
                        }
                    }
                    results.put("d3d", Display3D.get(common_ls));
                }
                this.result = results;
                Utils.log2("Done.");
            } catch (final Exception e) {
                IJError.print(e);
            } finally {
                finishedWorking();
            }
        }
    };
    return Bureaucrat.createAndStart(worker, p[0]);
}
Also used : CATAParameters(ini.trakem2.analysis.Compare.CATAParameters) ByteProcessor(ij.process.ByteProcessor) HashMap(java.util.HashMap) Color3f(org.scijava.vecmath.Color3f) ArrayList(java.util.ArrayList) FileSaver(ij.io.FileSaver) Worker(ini.trakem2.utils.Worker) IndexColorModel(java.awt.image.IndexColorModel) HashSet(java.util.HashSet) LayerSet(ini.trakem2.display.LayerSet) Display3D(ini.trakem2.display.Display3D) Plot(ij.gui.Plot) Color(java.awt.Color) ImagePlus(ij.ImagePlus) Project(ini.trakem2.Project) VectorString3D(ini.trakem2.vector.VectorString3D) Content(ij3d.Content) AffineTransform(java.awt.geom.AffineTransform) Map(java.util.Map) HashMap(java.util.HashMap) TreeMap(java.util.TreeMap) DirectoryChooser(ij.io.DirectoryChooser)

Example 5 with Plot

use of ij.gui.Plot in project TrakEM2 by trakem2.

the class Compare method makePlot.

public static Plot makePlot(final CATAParameters cp, final String name, final VectorString3D c) {
    final double[] stdDev = c.getStdDevAtEachPoint();
    if (null == stdDev)
        return null;
    final double[] index = new double[stdDev.length];
    for (int i = 0; i < index.length; i++) index[i] = i;
    Utils.log2("name is " + name);
    Utils.log2("c is " + c);
    Utils.log2("cp is " + cp);
    Utils.log2("stdDev is " + stdDev);
    Utils.log2("c.getCalibrationCopy() is " + c.getCalibrationCopy());
    Utils.log2("c.getDelta() is " + c.getDelta());
    final Calibration cal = c.getCalibrationCopy();
    if (null == cal)
        Utils.log2("WARNING null calibration!");
    final Plot plot = new Plot(name, name + " -- Point index (delta: " + Utils.cutNumber(c.getDelta(), 2) + " " + (null == cal ? "pixels" : cal.getUnits()) + ")", "Std Dev", index, stdDev);
    plot.setLimits(0, cp.plot_max_x, 0, cp.plot_max_y);
    plot.setSize(cp.plot_width, cp.plot_height);
    plot.setLineWidth(2);
    return plot;
}
Also used : Plot(ij.gui.Plot) Calibration(ij.measure.Calibration)

Aggregations

Plot (ij.gui.Plot)89 HistogramPlot (uk.ac.sussex.gdsc.core.ij.HistogramPlot)20 Point (java.awt.Point)19 PlotWindow (ij.gui.PlotWindow)17 Color (java.awt.Color)13 WindowOrganiser (uk.ac.sussex.gdsc.core.ij.plugin.WindowOrganiser)13 HistogramPlotBuilder (uk.ac.sussex.gdsc.core.ij.HistogramPlot.HistogramPlotBuilder)12 BasePoint (uk.ac.sussex.gdsc.core.match.BasePoint)12 ExtendedGenericDialog (uk.ac.sussex.gdsc.core.ij.gui.ExtendedGenericDialog)11 MemoryPeakResults (uk.ac.sussex.gdsc.smlm.results.MemoryPeakResults)11 Rectangle (java.awt.Rectangle)9 ArrayList (java.util.ArrayList)9 GenericDialog (ij.gui.GenericDialog)8 NonBlockingExtendedGenericDialog (uk.ac.sussex.gdsc.core.ij.gui.NonBlockingExtendedGenericDialog)7 LocalList (uk.ac.sussex.gdsc.core.utils.LocalList)7 Statistics (uk.ac.sussex.gdsc.core.utils.Statistics)7 StoredData (uk.ac.sussex.gdsc.core.utils.StoredData)7 StoredDataStatistics (uk.ac.sussex.gdsc.core.utils.StoredDataStatistics)7 ImagePlus (ij.ImagePlus)6 TDoubleArrayList (gnu.trove.list.array.TDoubleArrayList)5