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Example 26 with MemoryPeakResults

use of uk.ac.sussex.gdsc.smlm.results.MemoryPeakResults in project GDSC-SMLM by aherbert.

the class ResultsManager method load.

private static void load(String path) {
    // to get the calibration.
    if (Recorder.record) {
        Recorder.setCommand("Results Manager");
        Recorder.recordOption("input", INPUT_FILE);
        Recorder.recordOption("input_file", path);
        Recorder.recordOption("image", SettingsManager.getResultsImageTypeNames()[ResultsImageType.DRAW_NONE_VALUE]);
        Recorder.recordOption("results_file", "[]");
        Recorder.recordOption("save_to_memory");
    }
    final MemoryPeakResults results = loadInputResults(INPUT_FILE, true, null, null, new FilenameLoadOption(path));
    if (MemoryPeakResults.isEmpty(results)) {
        IJ.error(TITLE, "No results could be loaded from " + path);
    } else {
        if (addResultsToMemory(results, path) && Recorder.record) {
            Recorder.saveCommand();
        }
    }
}
Also used : MemoryPeakResults(uk.ac.sussex.gdsc.smlm.results.MemoryPeakResults)

Example 27 with MemoryPeakResults

use of uk.ac.sussex.gdsc.smlm.results.MemoryPeakResults in project GDSC-SMLM by aherbert.

the class ResultsManager method runClearMemory.

private static void runClearMemory(String arg) {
    if (MemoryPeakResults.isMemoryEmpty()) {
        IJ.error(TITLE, "There are no fitting results in memory");
        IJ.showStatus("");
        return;
    }
    Collection<MemoryPeakResults> allResults;
    boolean removeAll = false;
    String helpKey = "clear-memory-results";
    if (arg.contains("multi")) {
        helpKey += "-multi";
        final MultiDialog md = createMultiDialog(TITLE);
        md.setSelected(lastSelected.get());
        md.setHelpUrl(HelpUrls.getUrl(helpKey));
        md.showDialog();
        if (md.wasCancelled()) {
            return;
        }
        final List<String> selected = md.getSelectedResults();
        if (selected.isEmpty()) {
            return;
        }
        lastSelected.set(selected);
        allResults = new ArrayList<>(selected.size());
        for (final String name : selected) {
            final MemoryPeakResults r = MemoryPeakResults.getResults(name);
            if (r != null) {
                allResults.add(r);
            }
        }
    } else {
        removeAll = true;
        allResults = MemoryPeakResults.getAllResults();
    }
    if (allResults.isEmpty()) {
        return;
    }
    long memorySize = 0;
    int size = 0;
    for (final MemoryPeakResults results : allResults) {
        memorySize += MemoryPeakResults.estimateMemorySize(results);
        size += results.size();
    }
    final String memory = TextUtils.bytesToString(memorySize);
    final String count = TextUtils.pleural(size, "result");
    final String sets = TextUtils.pleural(allResults.size(), "set");
    final GenericDialog gd = new GenericDialog(TITLE);
    gd.addMessage(String.format("Do you want to remove %s from memory (%s, %s)?", count, sets, memory));
    gd.addHelp(HelpUrls.getUrl(helpKey));
    gd.showDialog();
    if (gd.wasCanceled()) {
        return;
    }
    if (removeAll) {
        MemoryPeakResults.clearMemory();
    } else {
        for (final MemoryPeakResults results : allResults) {
            MemoryPeakResults.removeResults(results.getName());
        }
    }
    SummariseResults.clearSummaryTable();
    ImageJUtils.log("Cleared %s (%s, %s)", count, sets, memory);
}
Also used : ExtendedGenericDialog(uk.ac.sussex.gdsc.core.ij.gui.ExtendedGenericDialog) GenericDialog(ij.gui.GenericDialog) MemoryPeakResults(uk.ac.sussex.gdsc.smlm.results.MemoryPeakResults) MultiDialog(uk.ac.sussex.gdsc.core.ij.gui.MultiDialog)

Example 28 with MemoryPeakResults

use of uk.ac.sussex.gdsc.smlm.results.MemoryPeakResults in project GDSC-SMLM by aherbert.

the class PeakFit method getSingleFrame.

/**
 * Gets the single frame containing all the results (if they are all in a single frame), else 0.
 *
 * @param results the results (must not be empty)
 * @return the single frame (or zero)
 */
private static int getSingleFrame(MemoryPeakResults results) {
    final FrameCounter counter = new FrameCounter(results.getFirstFrame());
    // The counter will return true (stop execution) if a new frame
    results.forEach((PeakResultProcedureX) peakResult -> counter.advance(peakResult.getFrame()));
    if (counter.currentFrame() != counter.previousFrame()) {
        return 0;
    }
    return counter.currentFrame();
}
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Example 29 with MemoryPeakResults

use of uk.ac.sussex.gdsc.smlm.results.MemoryPeakResults in project GDSC-SMLM by aherbert.

the class PeakFit method runMaximaFitting.

/**
 * Load the selected results from memory. All multiple frame results are added directly to the
 * results. All single frame results are added to a list of candidate maxima per frame and fitted
 * using the configured parameters.
 */
private void runMaximaFitting() {
    final MemoryPeakResults memoryResults = ResultsManager.loadInputResults(settings.inputOption, false, DistanceUnit.PIXEL);
    if (memoryResults == null || memoryResults.size() == 0) {
        log("No results for maxima fitting");
        return;
    }
    // The total frames (for progress reporting)
    int totalFrames;
    // A function that can convert a frame into a set of candidate indices
    final IntFunction<int[]> frameToMaxIndices;
    // The frames to process (should be sorted ascending)
    Supplier<IntStream> frames;
    // Support fitting all time frames with the same results.
    if (settings.fitAcrossAllFrames) {
        // Check if the input spans multiple frames
        if (getSingleFrame(memoryResults) == 0) {
            final int min = memoryResults.getMinFrame();
            final int max = memoryResults.getMaxFrame();
            final GenericDialog gd = new GenericDialog(TITLE);
            gd.enableYesNoCancel();
            gd.hideCancelButton();
            ImageJUtils.addMessage(gd, "Candidate maxima for fitting span multiple frames (%d-%d).\n \n" + "Please confirm the %s are correct.", min, max, TextUtils.pleural(memoryResults.size(), "candidate"));
            gd.showDialog();
            if (!gd.wasOKed()) {
                return;
            }
        }
        final int[] maxIndices = getMaxIndices(Arrays.asList(memoryResults.toArray()));
        // This may not work correctly if using for example a series image source that
        // incorrectly estimates the number of frames
        totalFrames = source.getFrames();
        frameToMaxIndices = frame -> maxIndices;
        frames = () -> IntStream.rangeClosed(1, totalFrames);
    } else {
        // Build a map between the time-frame and the results in that frame.
        final Map<Integer, List<PeakResult>> map = Arrays.stream(memoryResults.toArray()).parallel().filter(peakResult -> peakResult.getFrame() == peakResult.getEndFrame()).collect(Collectors.groupingBy(PeakResult::getFrame));
        totalFrames = map.size();
        // Build a function that can convert a frame into a set of candidate indices
        frameToMaxIndices = frame -> getMaxIndices(map.get(frame));
        frames = () -> map.keySet().stream().mapToInt(Integer::intValue).sorted();
    }
    final ImageStack stack = (extraSettings.showProcessedFrames) ? new ImageStack(bounds.width, bounds.height) : null;
    // Use the FitEngine to allow multi-threading.
    final FitEngine engine = createFitEngine(getNumberOfThreads(totalFrames));
    if (engine == null) {
        return;
    }
    final int step = ImageJUtils.getProgressInterval(totalFrames);
    // No crop bounds are supported.
    // To pre-process data for noise estimation
    final boolean isFitCameraCounts = fitConfig.isFitCameraCounts();
    final CameraModel cameraModel = fitConfig.getCameraModel();
    runTime = System.nanoTime();
    final AtomicBoolean shutdown = new AtomicBoolean();
    final String format = String.format("Slice: %%d / %d (Results=%%d)", totalFrames);
    frames.get().forEachOrdered(slice -> {
        if (shutdown.get() || escapePressed()) {
            shutdown.set(true);
            return;
        }
        final float[] data = source.get(slice);
        if (data == null) {
            shutdown.set(true);
            return;
        }
        if (slice % step == 0) {
            if (ImageJUtils.showStatus(() -> String.format(format, slice, results.size()))) {
                IJ.showProgress(slice, totalFrames);
            }
        }
        // We must pre-process the data before noise estimation
        final float[] data2 = data.clone();
        if (isFitCameraCounts) {
            cameraModel.removeBias(data2);
        } else {
            cameraModel.removeBiasAndGain(data2);
        }
        final float noise = FitWorker.estimateNoise(data2, source.getWidth(), source.getHeight(), config.getNoiseMethod());
        if (stack != null) {
            stack.addSlice(String.format("Frame %d - %d", source.getStartFrameNumber(), source.getEndFrameNumber()), data);
        }
        // Get the frame number from the source to allow for interlaced and aggregated data
        engine.run(createMaximaFitJob(frameToMaxIndices.apply(slice), source.getStartFrameNumber(), source.getEndFrameNumber(), data, bounds, noise));
    });
    engine.end(shutdown.get());
    time = engine.getTime();
    runTime = System.nanoTime() - runTime;
    if (stack != null) {
        ImageJUtils.display("Processed frames", stack);
    }
    showResults();
    source.close();
}
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Example 30 with MemoryPeakResults

use of uk.ac.sussex.gdsc.smlm.results.MemoryPeakResults in project GDSC-SMLM by aherbert.

the class ImageJ3DResultsViewer method getPoints.

/**
 * Gets the points.
 *
 * @param results the results
 * @param settings the settings
 * @return the points
 */
static LocalList<Point3f> getPoints(MemoryPeakResults results, ImageJ3DResultsViewerSettingsOrBuilder settings) {
    final LocalList<Point3f> points = new LocalList<>(results.size());
    if (results.is3D()) {
        results.forEach(DistanceUnit.NM, (XyzResultProcedure) (x, y, z) -> {
            points.push(new Point3f(x, y, z));
        });
    } else {
        results.forEach(DistanceUnit.NM, (XyResultProcedure) (x, y) -> {
            points.push(new Point3f(x, y, 0));
        });
        final double range = settings.getDepthRange();
        if (range > 0 && results.size() > 1) {
            final DepthMode mode = DepthMode.forNumber(settings.getDepthMode());
            final double min = -settings.getDepthRange() / 2;
            switch(mode) {
                case DITHER:
                    final SplitMix r = SplitMix.new64(settings.getDitherSeed());
                    for (int i = points.size(); i-- > 0; ) {
                        points.unsafeGet(i).z += (min + r.nextDouble() * range);
                    }
                    break;
                case INTENSITY:
                    // Rank by intensity, highest first
                    final StandardResultProcedure p = new StandardResultProcedure(results);
                    p.getI();
                    final int[] indices = SimpleArrayUtils.natural(results.size());
                    SortUtils.sortIndices(indices, p.intensity, true);
                    final double inc = range / indices.length;
                    for (int i = 0; i < indices.length; i++) {
                        // The standard rendering has +z going away so put the highest rank at min
                        points.unsafeGet(indices[i]).z += (min + i * inc);
                    }
                    break;
                case NONE:
                    break;
                default:
                    throw new IllegalStateException("Unknown depth mode: " + mode);
            }
        }
    }
    return points;
}
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Aggregations

MemoryPeakResults (uk.ac.sussex.gdsc.smlm.results.MemoryPeakResults)138 PeakResult (uk.ac.sussex.gdsc.smlm.results.PeakResult)61 List (java.util.List)47 ExtendedGenericDialog (uk.ac.sussex.gdsc.core.ij.gui.ExtendedGenericDialog)46 IJ (ij.IJ)39 DistanceUnit (uk.ac.sussex.gdsc.smlm.data.config.UnitProtos.DistanceUnit)39 ImageJUtils (uk.ac.sussex.gdsc.core.ij.ImageJUtils)38 PeakResultProcedure (uk.ac.sussex.gdsc.smlm.results.procedures.PeakResultProcedure)38 ArrayList (java.util.ArrayList)36 AtomicReference (java.util.concurrent.atomic.AtomicReference)36 PlugIn (ij.plugin.PlugIn)34 MathUtils (uk.ac.sussex.gdsc.core.utils.MathUtils)33 Counter (uk.ac.sussex.gdsc.smlm.results.count.Counter)33 ImagePlus (ij.ImagePlus)31 Rectangle (java.awt.Rectangle)31 SimpleArrayUtils (uk.ac.sussex.gdsc.core.utils.SimpleArrayUtils)31 LocalList (uk.ac.sussex.gdsc.core.utils.LocalList)28 TextUtils (uk.ac.sussex.gdsc.core.utils.TextUtils)28 SettingsManager (uk.ac.sussex.gdsc.smlm.ij.settings.SettingsManager)28 FrameCounter (uk.ac.sussex.gdsc.smlm.results.count.FrameCounter)28