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Example 11 with Filter

use of gdsc.smlm.results.filter.Filter in project GDSC-SMLM by aherbert.

the class BenchmarkSpotFit method summariseResults.

private void summariseResults(TIntObjectHashMap<FilterCandidates> filterCandidates, long runTime, final PreprocessedPeakResult[] preprocessedPeakResults, int nUniqueIDs) {
    createTable();
    // Summarise the fitting results. N fits, N failures. 
    // Optimal match statistics if filtering is perfect (since fitting is not perfect).
    StoredDataStatistics distanceStats = new StoredDataStatistics();
    StoredDataStatistics depthStats = new StoredDataStatistics();
    // Get stats for all fitted results and those that match 
    // Signal, SNR, Width, xShift, yShift, Precision
    createFilterCriteria();
    StoredDataStatistics[][] stats = new StoredDataStatistics[3][filterCriteria.length];
    for (int i = 0; i < stats.length; i++) for (int j = 0; j < stats[i].length; j++) stats[i][j] = new StoredDataStatistics();
    final double nmPerPixel = simulationParameters.a;
    double tp = 0, fp = 0;
    int failcTP = 0, failcFP = 0;
    int cTP = 0, cFP = 0;
    int[] singleStatus = null, multiStatus = null, doubletStatus = null, multiDoubletStatus = null;
    singleStatus = new int[FitStatus.values().length];
    multiStatus = new int[singleStatus.length];
    doubletStatus = new int[singleStatus.length];
    multiDoubletStatus = new int[singleStatus.length];
    // Easier to materialise the values since we have a lot of non final variables to manipulate
    final int[] frames = new int[filterCandidates.size()];
    final FilterCandidates[] candidates = new FilterCandidates[filterCandidates.size()];
    final int[] counter = new int[1];
    filterCandidates.forEachEntry(new TIntObjectProcedure<FilterCandidates>() {

        public boolean execute(int a, FilterCandidates b) {
            frames[counter[0]] = a;
            candidates[counter[0]] = b;
            counter[0]++;
            return true;
        }
    });
    for (FilterCandidates result : candidates) {
        // Count the number of fit results that matched (tp) and did not match (fp)
        tp += result.tp;
        fp += result.fp;
        for (int i = 0; i < result.fitResult.length; i++) {
            if (result.spots[i].match)
                cTP++;
            else
                cFP++;
            final MultiPathFitResult fitResult = result.fitResult[i];
            if (singleStatus != null && result.spots[i].match) {
                // Debugging reasons for fit failure
                addStatus(singleStatus, fitResult.getSingleFitResult());
                addStatus(multiStatus, fitResult.getMultiFitResult());
                addStatus(doubletStatus, fitResult.getDoubletFitResult());
                addStatus(multiDoubletStatus, fitResult.getMultiDoubletFitResult());
            }
            if (noMatch(fitResult)) {
                if (result.spots[i].match)
                    failcTP++;
                else
                    failcFP++;
            }
            // We have multi-path results.
            // We want statistics for:
            // [0] all fitted spots
            // [1] fitted spots that match a result
            // [2] fitted spots that do not match a result
            addToStats(fitResult.getSingleFitResult(), stats);
            addToStats(fitResult.getMultiFitResult(), stats);
            addToStats(fitResult.getDoubletFitResult(), stats);
            addToStats(fitResult.getMultiDoubletFitResult(), stats);
        }
        // Statistics on spots that fit an actual result
        for (int i = 0; i < result.match.length; i++) {
            if (!result.match[i].isFitResult())
                // For now just ignore the candidates that matched
                continue;
            FitMatch fitMatch = (FitMatch) result.match[i];
            distanceStats.add(fitMatch.d * nmPerPixel);
            depthStats.add(fitMatch.z * nmPerPixel);
        }
    }
    // Store data for computing correlation
    double[] i1 = new double[depthStats.getN()];
    double[] i2 = new double[i1.length];
    double[] is = new double[i1.length];
    int ci = 0;
    for (FilterCandidates result : candidates) {
        for (int i = 0; i < result.match.length; i++) {
            if (!result.match[i].isFitResult())
                // For now just ignore the candidates that matched
                continue;
            FitMatch fitMatch = (FitMatch) result.match[i];
            ScoredSpot spot = result.spots[fitMatch.i];
            i1[ci] = fitMatch.predictedSignal;
            i2[ci] = fitMatch.actualSignal;
            is[ci] = spot.spot.intensity;
            ci++;
        }
    }
    // We want to compute the Jaccard against the spot metric
    // Filter the results using the multi-path filter
    ArrayList<MultiPathFitResults> multiPathResults = new ArrayList<MultiPathFitResults>(filterCandidates.size());
    for (int i = 0; i < frames.length; i++) {
        int frame = frames[i];
        MultiPathFitResult[] multiPathFitResults = candidates[i].fitResult;
        int totalCandidates = candidates[i].spots.length;
        int nActual = actualCoordinates.get(frame).size();
        multiPathResults.add(new MultiPathFitResults(frame, multiPathFitResults, totalCandidates, nActual));
    }
    // Score the results and count the number returned
    List<FractionalAssignment[]> assignments = new ArrayList<FractionalAssignment[]>();
    final TIntHashSet set = new TIntHashSet(nUniqueIDs);
    FractionScoreStore scoreStore = new FractionScoreStore() {

        public void add(int uniqueId) {
            set.add(uniqueId);
        }
    };
    MultiPathFitResults[] multiResults = multiPathResults.toArray(new MultiPathFitResults[multiPathResults.size()]);
    // Filter with no filter
    MultiPathFilter mpf = new MultiPathFilter(new SignalFilter(0), null, multiFilter.residualsThreshold);
    FractionClassificationResult fractionResult = mpf.fractionScoreSubset(multiResults, Integer.MAX_VALUE, this.results.size(), assignments, scoreStore, CoordinateStoreFactory.create(imp.getWidth(), imp.getHeight(), fitConfig.getDuplicateDistance()));
    double nPredicted = fractionResult.getTP() + fractionResult.getFP();
    final double[][] matchScores = new double[set.size()][];
    int count = 0;
    for (int i = 0; i < assignments.size(); i++) {
        FractionalAssignment[] a = assignments.get(i);
        if (a == null)
            continue;
        for (int j = 0; j < a.length; j++) {
            final PreprocessedPeakResult r = ((PeakFractionalAssignment) a[j]).peakResult;
            set.remove(r.getUniqueId());
            final double precision = Math.sqrt(r.getLocationVariance());
            final double signal = r.getSignal();
            final double snr = r.getSNR();
            final double width = r.getXSDFactor();
            final double xShift = r.getXRelativeShift2();
            final double yShift = r.getYRelativeShift2();
            // Since these two are combined for filtering and the max is what matters.
            final double shift = (xShift > yShift) ? Math.sqrt(xShift) : Math.sqrt(yShift);
            final double eshift = Math.sqrt(xShift + yShift);
            final double[] score = new double[8];
            score[FILTER_SIGNAL] = signal;
            score[FILTER_SNR] = snr;
            score[FILTER_MIN_WIDTH] = width;
            score[FILTER_MAX_WIDTH] = width;
            score[FILTER_SHIFT] = shift;
            score[FILTER_ESHIFT] = eshift;
            score[FILTER_PRECISION] = precision;
            score[FILTER_PRECISION + 1] = a[j].getScore();
            matchScores[count++] = score;
        }
    }
    // Add the rest
    set.forEach(new CustomTIntProcedure(count) {

        public boolean execute(int uniqueId) {
            // This should not be null or something has gone wrong
            PreprocessedPeakResult r = preprocessedPeakResults[uniqueId];
            if (r == null)
                throw new RuntimeException("Missing result: " + uniqueId);
            final double precision = Math.sqrt(r.getLocationVariance());
            final double signal = r.getSignal();
            final double snr = r.getSNR();
            final double width = r.getXSDFactor();
            final double xShift = r.getXRelativeShift2();
            final double yShift = r.getYRelativeShift2();
            // Since these two are combined for filtering and the max is what matters.
            final double shift = (xShift > yShift) ? Math.sqrt(xShift) : Math.sqrt(yShift);
            final double eshift = Math.sqrt(xShift + yShift);
            final double[] score = new double[8];
            score[FILTER_SIGNAL] = signal;
            score[FILTER_SNR] = snr;
            score[FILTER_MIN_WIDTH] = width;
            score[FILTER_MAX_WIDTH] = width;
            score[FILTER_SHIFT] = shift;
            score[FILTER_ESHIFT] = eshift;
            score[FILTER_PRECISION] = precision;
            matchScores[c++] = score;
            return true;
        }
    });
    // Debug the reasons the fit failed
    if (singleStatus != null) {
        String name = PeakFit.getSolverName(fitConfig);
        if (fitConfig.getFitSolver() == FitSolver.MLE && fitConfig.isModelCamera())
            name += " Camera";
        System.out.println("Failure counts: " + name);
        printFailures("Single", singleStatus);
        printFailures("Multi", multiStatus);
        printFailures("Doublet", doubletStatus);
        printFailures("Multi doublet", multiDoubletStatus);
    }
    StringBuilder sb = new StringBuilder(300);
    // Add information about the simulation
    //(simulationParameters.minSignal + simulationParameters.maxSignal) * 0.5;
    final double signal = simulationParameters.signalPerFrame;
    final int n = results.size();
    sb.append(imp.getStackSize()).append("\t");
    final int w = imp.getWidth();
    final int h = imp.getHeight();
    sb.append(w).append("\t");
    sb.append(h).append("\t");
    sb.append(n).append("\t");
    double density = ((double) n / imp.getStackSize()) / (w * h) / (simulationParameters.a * simulationParameters.a / 1e6);
    sb.append(Utils.rounded(density)).append("\t");
    sb.append(Utils.rounded(signal)).append("\t");
    sb.append(Utils.rounded(simulationParameters.s)).append("\t");
    sb.append(Utils.rounded(simulationParameters.a)).append("\t");
    sb.append(Utils.rounded(simulationParameters.depth)).append("\t");
    sb.append(simulationParameters.fixedDepth).append("\t");
    sb.append(Utils.rounded(simulationParameters.gain)).append("\t");
    sb.append(Utils.rounded(simulationParameters.readNoise)).append("\t");
    sb.append(Utils.rounded(simulationParameters.b)).append("\t");
    sb.append(Utils.rounded(simulationParameters.b2)).append("\t");
    // Compute the noise
    double noise = simulationParameters.b2;
    if (simulationParameters.emCCD) {
        // The b2 parameter was computed without application of the EM-CCD noise factor of 2.
        //final double b2 = backgroundVariance + readVariance
        //                = simulationParameters.b + readVariance
        // This should be applied only to the background variance.
        final double readVariance = noise - simulationParameters.b;
        noise = simulationParameters.b * 2 + readVariance;
    }
    if (simulationParameters.fullSimulation) {
    // The total signal is spread over frames
    }
    sb.append(Utils.rounded(signal / Math.sqrt(noise))).append("\t");
    sb.append(Utils.rounded(simulationParameters.s / simulationParameters.a)).append("\t");
    sb.append(spotFilter.getDescription());
    // nP and nN is the fractional score of the spot candidates 
    addCount(sb, nP + nN);
    addCount(sb, nP);
    addCount(sb, nN);
    addCount(sb, fP);
    addCount(sb, fN);
    String name = PeakFit.getSolverName(fitConfig);
    if (fitConfig.getFitSolver() == FitSolver.MLE && fitConfig.isModelCamera())
        name += " Camera";
    add(sb, name);
    add(sb, config.getFitting());
    resultPrefix = sb.toString();
    // Q. Should I add other fit configuration here?
    // The fraction of positive and negative candidates that were included
    add(sb, (100.0 * cTP) / nP);
    add(sb, (100.0 * cFP) / nN);
    // Score the fitting results compared to the original simulation.
    // Score the candidate selection:
    add(sb, cTP + cFP);
    add(sb, cTP);
    add(sb, cFP);
    // TP are all candidates that can be matched to a spot
    // FP are all candidates that cannot be matched to a spot
    // FN = The number of missed spots
    FractionClassificationResult m = new FractionClassificationResult(cTP, cFP, 0, simulationParameters.molecules - cTP);
    add(sb, m.getRecall());
    add(sb, m.getPrecision());
    add(sb, m.getF1Score());
    add(sb, m.getJaccard());
    // Score the fitting results:
    add(sb, failcTP);
    add(sb, failcFP);
    // TP are all fit results that can be matched to a spot
    // FP are all fit results that cannot be matched to a spot
    // FN = The number of missed spots
    add(sb, tp);
    add(sb, fp);
    m = new FractionClassificationResult(tp, fp, 0, simulationParameters.molecules - tp);
    add(sb, m.getRecall());
    add(sb, m.getPrecision());
    add(sb, m.getF1Score());
    add(sb, m.getJaccard());
    // Do it again but pretend we can perfectly filter all the false positives
    //add(sb, tp);
    m = new FractionClassificationResult(tp, 0, 0, simulationParameters.molecules - tp);
    // Recall is unchanged
    // Precision will be 100%
    add(sb, m.getF1Score());
    add(sb, m.getJaccard());
    // The mean may be subject to extreme outliers so use the median
    double median = distanceStats.getMedian();
    add(sb, median);
    WindowOrganiser wo = new WindowOrganiser();
    String label = String.format("Recall = %s. n = %d. Median = %s nm. SD = %s nm", Utils.rounded(m.getRecall()), distanceStats.getN(), Utils.rounded(median), Utils.rounded(distanceStats.getStandardDeviation()));
    int id = Utils.showHistogram(TITLE, distanceStats, "Match Distance (nm)", 0, 0, 0, label);
    if (Utils.isNewWindow())
        wo.add(id);
    median = depthStats.getMedian();
    add(sb, median);
    // Sort by spot intensity and produce correlation
    int[] indices = Utils.newArray(i1.length, 0, 1);
    if (showCorrelation)
        Sort.sort(indices, is, rankByIntensity);
    double[] r = (showCorrelation) ? new double[i1.length] : null;
    double[] sr = (showCorrelation) ? new double[i1.length] : null;
    double[] rank = (showCorrelation) ? new double[i1.length] : null;
    ci = 0;
    FastCorrelator fastCorrelator = new FastCorrelator();
    ArrayList<Ranking> pc1 = new ArrayList<Ranking>();
    ArrayList<Ranking> pc2 = new ArrayList<Ranking>();
    for (int ci2 : indices) {
        fastCorrelator.add((long) Math.round(i1[ci2]), (long) Math.round(i2[ci2]));
        pc1.add(new Ranking(i1[ci2], ci));
        pc2.add(new Ranking(i2[ci2], ci));
        if (showCorrelation) {
            r[ci] = fastCorrelator.getCorrelation();
            sr[ci] = Correlator.correlation(rank(pc1), rank(pc2));
            if (rankByIntensity)
                rank[ci] = is[0] - is[ci];
            else
                rank[ci] = ci;
        }
        ci++;
    }
    final double pearsonCorr = fastCorrelator.getCorrelation();
    final double rankedCorr = Correlator.correlation(rank(pc1), rank(pc2));
    // Get the regression
    SimpleRegression regression = new SimpleRegression(false);
    for (int i = 0; i < pc1.size(); i++) regression.addData(pc1.get(i).value, pc2.get(i).value);
    //final double intercept = regression.getIntercept();
    final double slope = regression.getSlope();
    if (showCorrelation) {
        String title = TITLE + " Intensity";
        Plot plot = new Plot(title, "Candidate", "Spot");
        double[] limits1 = Maths.limits(i1);
        double[] limits2 = Maths.limits(i2);
        plot.setLimits(limits1[0], limits1[1], limits2[0], limits2[1]);
        label = String.format("Correlation=%s; Ranked=%s; Slope=%s", Utils.rounded(pearsonCorr), Utils.rounded(rankedCorr), Utils.rounded(slope));
        plot.addLabel(0, 0, label);
        plot.setColor(Color.red);
        plot.addPoints(i1, i2, Plot.DOT);
        if (slope > 1)
            plot.drawLine(limits1[0], limits1[0] * slope, limits1[1], limits1[1] * slope);
        else
            plot.drawLine(limits2[0] / slope, limits2[0], limits2[1] / slope, limits2[1]);
        PlotWindow pw = Utils.display(title, plot);
        if (Utils.isNewWindow())
            wo.add(pw);
        title = TITLE + " Correlation";
        plot = new Plot(title, "Spot Rank", "Correlation");
        double[] xlimits = Maths.limits(rank);
        double[] ylimits = Maths.limits(r);
        ylimits = Maths.limits(ylimits, sr);
        plot.setLimits(xlimits[0], xlimits[1], ylimits[0], ylimits[1]);
        plot.setColor(Color.red);
        plot.addPoints(rank, r, Plot.LINE);
        plot.setColor(Color.blue);
        plot.addPoints(rank, sr, Plot.LINE);
        plot.setColor(Color.black);
        plot.addLabel(0, 0, label);
        pw = Utils.display(title, plot);
        if (Utils.isNewWindow())
            wo.add(pw);
    }
    add(sb, pearsonCorr);
    add(sb, rankedCorr);
    add(sb, slope);
    label = String.format("n = %d. Median = %s nm", depthStats.getN(), Utils.rounded(median));
    id = Utils.showHistogram(TITLE, depthStats, "Match Depth (nm)", 0, 1, 0, label);
    if (Utils.isNewWindow())
        wo.add(id);
    // Plot histograms of the stats on the same window
    double[] lower = new double[filterCriteria.length];
    double[] upper = new double[lower.length];
    min = new double[lower.length];
    max = new double[lower.length];
    for (int i = 0; i < stats[0].length; i++) {
        double[] limits = showDoubleHistogram(stats, i, wo, matchScores, nPredicted);
        lower[i] = limits[0];
        upper[i] = limits[1];
        min[i] = limits[2];
        max[i] = limits[3];
    }
    // Reconfigure some of the range limits
    // Make this a bit bigger
    upper[FILTER_SIGNAL] *= 2;
    // Make this a bit bigger
    upper[FILTER_SNR] *= 2;
    double factor = 0.25;
    if (lower[FILTER_MIN_WIDTH] != 0)
        // (assuming lower is less than 1)
        upper[FILTER_MIN_WIDTH] = 1 - Math.max(0, factor * (1 - lower[FILTER_MIN_WIDTH]));
    if (upper[FILTER_MIN_WIDTH] != 0)
        // (assuming upper is more than 1)
        lower[FILTER_MAX_WIDTH] = 1 + Math.max(0, factor * (upper[FILTER_MAX_WIDTH] - 1));
    // Round the ranges
    final double[] interval = new double[stats[0].length];
    interval[FILTER_SIGNAL] = SignalFilter.DEFAULT_INCREMENT;
    interval[FILTER_SNR] = SNRFilter.DEFAULT_INCREMENT;
    interval[FILTER_MIN_WIDTH] = WidthFilter2.DEFAULT_MIN_INCREMENT;
    interval[FILTER_MAX_WIDTH] = WidthFilter.DEFAULT_INCREMENT;
    interval[FILTER_SHIFT] = ShiftFilter.DEFAULT_INCREMENT;
    interval[FILTER_ESHIFT] = EShiftFilter.DEFAULT_INCREMENT;
    interval[FILTER_PRECISION] = PrecisionFilter.DEFAULT_INCREMENT;
    interval[FILTER_ITERATIONS] = 0.1;
    interval[FILTER_EVALUATIONS] = 0.1;
    // Create a range increment
    double[] increment = new double[lower.length];
    for (int i = 0; i < increment.length; i++) {
        lower[i] = Maths.floor(lower[i], interval[i]);
        upper[i] = Maths.ceil(upper[i], interval[i]);
        double range = upper[i] - lower[i];
        // Allow clipping if the range is small compared to the min increment
        double multiples = range / interval[i];
        // Use 8 multiples for the equivalent of +/- 4 steps around the centre
        if (multiples < 8) {
            multiples = Math.ceil(multiples);
        } else
            multiples = 8;
        increment[i] = Maths.ceil(range / multiples, interval[i]);
        if (i == FILTER_MIN_WIDTH)
            // Requires clipping based on the upper limit
            lower[i] = upper[i] - increment[i] * multiples;
        else
            upper[i] = lower[i] + increment[i] * multiples;
    }
    for (int i = 0; i < stats[0].length; i++) {
        lower[i] = Maths.round(lower[i]);
        upper[i] = Maths.round(upper[i]);
        min[i] = Maths.round(min[i]);
        max[i] = Maths.round(max[i]);
        increment[i] = Maths.round(increment[i]);
        sb.append("\t").append(min[i]).append(':').append(lower[i]).append('-').append(upper[i]).append(':').append(max[i]);
    }
    // Disable some filters
    increment[FILTER_SIGNAL] = Double.POSITIVE_INFINITY;
    //increment[FILTER_SHIFT] = Double.POSITIVE_INFINITY;
    increment[FILTER_ESHIFT] = Double.POSITIVE_INFINITY;
    wo.tile();
    sb.append("\t").append(Utils.timeToString(runTime / 1000000.0));
    summaryTable.append(sb.toString());
    if (saveFilterRange) {
        GlobalSettings gs = SettingsManager.loadSettings();
        FilterSettings filterSettings = gs.getFilterSettings();
        String filename = (silent) ? filterSettings.filterSetFilename : Utils.getFilename("Filter_range_file", filterSettings.filterSetFilename);
        if (filename == null)
            return;
        // Remove extension to store the filename
        filename = Utils.replaceExtension(filename, ".xml");
        filterSettings.filterSetFilename = filename;
        // Create a filter set using the ranges
        ArrayList<Filter> filters = new ArrayList<Filter>(3);
        filters.add(new MultiFilter2(lower[0], (float) lower[1], lower[2], lower[3], lower[4], lower[5], lower[6]));
        filters.add(new MultiFilter2(upper[0], (float) upper[1], upper[2], upper[3], upper[4], upper[5], upper[6]));
        filters.add(new MultiFilter2(increment[0], (float) increment[1], increment[2], increment[3], increment[4], increment[5], increment[6]));
        if (saveFilters(filename, filters))
            SettingsManager.saveSettings(gs);
        // Create a filter set using the min/max and the initial bounds.
        // Set sensible limits
        min[FILTER_SIGNAL] = Math.max(min[FILTER_SIGNAL], 30);
        max[FILTER_PRECISION] = Math.min(max[FILTER_PRECISION], 100);
        // Commented this out so that the 4-set filters are the same as the 3-set filters.
        // The difference leads to differences when optimising.
        //			// Use half the initial bounds (hoping this is a good starting guess for the optimum)
        //			final boolean[] limitToLower = new boolean[min.length];
        //			limitToLower[FILTER_SIGNAL] = true;
        //			limitToLower[FILTER_SNR] = true;
        //			limitToLower[FILTER_MIN_WIDTH] = true;
        //			limitToLower[FILTER_MAX_WIDTH] = false;
        //			limitToLower[FILTER_SHIFT] = false;
        //			limitToLower[FILTER_ESHIFT] = false;
        //			limitToLower[FILTER_PRECISION] = true;
        //			for (int i = 0; i < limitToLower.length; i++)
        //			{
        //				final double range = (upper[i] - lower[i]) / 2;
        //				if (limitToLower[i])
        //					upper[i] = lower[i] + range;
        //				else
        //					lower[i] = upper[i] - range;
        //			}
        filters = new ArrayList<Filter>(4);
        filters.add(new MultiFilter2(min[0], (float) min[1], min[2], min[3], min[4], min[5], min[6]));
        filters.add(new MultiFilter2(lower[0], (float) lower[1], lower[2], lower[3], lower[4], lower[5], lower[6]));
        filters.add(new MultiFilter2(upper[0], (float) upper[1], upper[2], upper[3], upper[4], upper[5], upper[6]));
        filters.add(new MultiFilter2(max[0], (float) max[1], max[2], max[3], max[4], max[5], max[6]));
        saveFilters(Utils.replaceExtension(filename, ".4.xml"), filters);
    }
}
Also used : ArrayList(java.util.ArrayList) TIntHashSet(gnu.trove.set.hash.TIntHashSet) MultiPathFitResult(gdsc.smlm.results.filter.MultiPathFitResult) FractionalAssignment(gdsc.core.match.FractionalAssignment) PeakFractionalAssignment(gdsc.smlm.results.filter.PeakFractionalAssignment) ImmutableFractionalAssignment(gdsc.core.match.ImmutableFractionalAssignment) FractionClassificationResult(gdsc.core.match.FractionClassificationResult) BasePreprocessedPeakResult(gdsc.smlm.results.filter.BasePreprocessedPeakResult) PreprocessedPeakResult(gdsc.smlm.results.filter.PreprocessedPeakResult) SignalFilter(gdsc.smlm.results.filter.SignalFilter) FilterSettings(gdsc.smlm.ij.settings.FilterSettings) ScoredSpot(gdsc.smlm.ij.plugins.BenchmarkSpotFilter.ScoredSpot) FastCorrelator(gdsc.core.utils.FastCorrelator) Plot(ij.gui.Plot) StoredDataStatistics(gdsc.core.utils.StoredDataStatistics) PlotWindow(ij.gui.PlotWindow) GlobalSettings(gdsc.smlm.ij.settings.GlobalSettings) WindowOrganiser(ij.plugin.WindowOrganiser) PeakResultPoint(gdsc.smlm.ij.plugins.ResultsMatchCalculator.PeakResultPoint) BasePoint(gdsc.core.match.BasePoint) PeakFractionalAssignment(gdsc.smlm.results.filter.PeakFractionalAssignment) FractionScoreStore(gdsc.smlm.results.filter.MultiPathFilter.FractionScoreStore) SimpleRegression(org.apache.commons.math3.stat.regression.SimpleRegression) SignalFilter(gdsc.smlm.results.filter.SignalFilter) DirectFilter(gdsc.smlm.results.filter.DirectFilter) ShiftFilter(gdsc.smlm.results.filter.ShiftFilter) PrecisionFilter(gdsc.smlm.results.filter.PrecisionFilter) Filter(gdsc.smlm.results.filter.Filter) EShiftFilter(gdsc.smlm.results.filter.EShiftFilter) WidthFilter(gdsc.smlm.results.filter.WidthFilter) SNRFilter(gdsc.smlm.results.filter.SNRFilter) MultiPathFilter(gdsc.smlm.results.filter.MultiPathFilter) MaximaSpotFilter(gdsc.smlm.filters.MaximaSpotFilter) MultiFilter2(gdsc.smlm.results.filter.MultiFilter2) MultiPathFitResults(gdsc.smlm.results.filter.MultiPathFitResults) MultiPathFilter(gdsc.smlm.results.filter.MultiPathFilter)

Example 12 with Filter

use of gdsc.smlm.results.filter.Filter in project GDSC-SMLM by aherbert.

the class BenchmarkFilterAnalysis method expandFilters.

/**
	 * Expand filters. Set the increment for any parameter not expanded to zero. Set the input parameters array to the
	 * lower bounds. Set the input parameters2 array to the upper bounds.
	 * <p>
	 * If a parameter is not expanded since the increment is Infinity the parameter is disabled. If it was not expanded
	 * for any other reason (increment is zero, NaN values, etc) the weakest parameter of the two input array is used
	 * and set as the lower and upper bounds.
	 *
	 * @param baseFilter
	 *            the base filter
	 * @param parameters
	 *            the parameters
	 * @param parameters2
	 *            the parameters 2
	 * @param increment
	 *            the increment
	 * @return the list
	 */
private static List<Filter> expandFilters(Filter baseFilter, double[] parameters, double[] parameters2, double[] increment) {
    final int n = baseFilter.getNumberOfParameters();
    if (parameters.length < n || parameters2.length < n || increment.length < n)
        throw new IllegalArgumentException("Input arrays  must be  at least the length of the number of parameters");
    double[] increment2 = increment.clone();
    int capacity = (int) countCombinations(parameters, parameters2, increment);
    ArrayList<Filter> list = new ArrayList<Filter>(capacity);
    // Initialise with a filter set at the minimum for each parameter.
    // Get the weakest parameters for those not expanded.
    Filter f1 = baseFilter.create(parameters);
    final double[] p = parameters2.clone();
    f1.weakestParameters(p);
    for (int i = 0; i < n; i++) {
        if (increment[i] == 0)
            // Disable if Infinite increment, otherwise use the weakest parameter
            parameters[i] = parameters2[i] = (Double.isInfinite(increment2[i])) ? baseFilter.getDisabledParameterValue(i) : p[i];
    }
    f1 = baseFilter.create(parameters);
    list.add(f1);
    for (int i = 0; i < n; i++) {
        if (increment[i] == 0)
            continue;
        final double min = parameters[i];
        final double max = parameters2[i];
        final double inc = increment[i];
        final double max2 = max + inc;
        // Set the upper bounds for the output and store the expansion params
        final StoredDataStatistics stats = new StoredDataStatistics(10);
        for (double value = min + inc; value < max || value - max < max2 - value; value += inc) {
            parameters2[i] = value;
            stats.add(value);
        }
        final double[] values = stats.getValues();
        final ArrayList<Filter> list2 = new ArrayList<Filter>((values.length + 1) * list.size());
        for (int k = 0; k < list.size(); k++) {
            final Filter f = list.get(k);
            // Copy params of the filter
            for (int j = 0; j < n; j++) p[j] = f.getParameterValue(j);
            for (int l = 0; l < values.length; l++) {
                p[i] = values[l];
                list2.add(f.create(p));
            }
        }
        list.addAll(list2);
    }
    // Sort the filters
    Collections.sort(list);
    return list;
}
Also used : IDirectFilter(gdsc.smlm.results.filter.IDirectFilter) DirectFilter(gdsc.smlm.results.filter.DirectFilter) Filter(gdsc.smlm.results.filter.Filter) MultiPathFilter(gdsc.smlm.results.filter.MultiPathFilter) MaximaSpotFilter(gdsc.smlm.filters.MaximaSpotFilter) ArrayList(java.util.ArrayList) StoredDataStatistics(gdsc.core.utils.StoredDataStatistics)

Example 13 with Filter

use of gdsc.smlm.results.filter.Filter in project GDSC-SMLM by aherbert.

the class BenchmarkFilterAnalysis method saveFilter.

private void saveFilter(DirectFilter filter) {
    // Save the filter to file
    String filename = getFilename("Best_Filter_File", filterFilename);
    if (filename != null) {
        filterFilename = filename;
        Prefs.set(KEY_FILTER_FILENAME, filename);
        List<Filter> filters = new ArrayList<Filter>(1);
        filters.add(filter);
        FilterSet filterSet = new FilterSet(filter.getName(), filters);
        List<FilterSet> list = new ArrayList<FilterSet>(1);
        list.add(filterSet);
        saveFilterSet(filterSet, filename);
    }
}
Also used : FilterSet(gdsc.smlm.results.filter.FilterSet) IDirectFilter(gdsc.smlm.results.filter.IDirectFilter) DirectFilter(gdsc.smlm.results.filter.DirectFilter) Filter(gdsc.smlm.results.filter.Filter) MultiPathFilter(gdsc.smlm.results.filter.MultiPathFilter) MaximaSpotFilter(gdsc.smlm.filters.MaximaSpotFilter) ArrayList(java.util.ArrayList)

Example 14 with Filter

use of gdsc.smlm.results.filter.Filter in project GDSC-SMLM by aherbert.

the class FreeFilterResults method run.

/*
	 * (non-)
	 * 
	 * @see ij.plugin.PlugIn#run(java.lang.String)
	 */
public void run(String arg) {
    SMLMUsageTracker.recordPlugin(this.getClass(), arg);
    if (MemoryPeakResults.isMemoryEmpty()) {
        // Ask user if they want to show the demo filters
        ExtendedGenericDialog gd = new ExtendedGenericDialog(TITLE);
        gd.enableYesNoCancel();
        gd.hideCancelButton();
        gd.addMessage("No results in memory. Show the demo filters?");
        gd.showDialog();
        if (gd.wasOKed())
            logDemoFilters(TITLE);
        return;
    }
    if (!showDialog())
        return;
    results = ResultsManager.loadInputResults(inputOption, false);
    if (results == null || results.size() == 0) {
        IJ.error(TITLE, "No results could be loaded");
        IJ.showStatus("");
        return;
    }
    // Filter results
    Filter filter = Filter.fromXML(filterSettings.freeFilter);
    if (filter != null) {
        MemoryPeakResults newResults = filter.filter(results);
        if (newResults.size() > 0) {
            newResults.setName(results.getName() + " Free Filtered");
            MemoryPeakResults.addResults(newResults);
        }
        IJ.showStatus(String.format("Filtered %d results to %d", results.size(), newResults.size()));
    } else {
        IJ.showStatus("ERROR: Unable to create filter");
    }
}
Also used : Filter(gdsc.smlm.results.filter.Filter) OrFilter(gdsc.smlm.results.filter.OrFilter) AndFilter(gdsc.smlm.results.filter.AndFilter) EShiftFilter(gdsc.smlm.results.filter.EShiftFilter) ShiftFilter(gdsc.smlm.results.filter.ShiftFilter) SignalFilter(gdsc.smlm.results.filter.SignalFilter) MultiFilter(gdsc.smlm.results.filter.MultiFilter) SBRFilter(gdsc.smlm.results.filter.SBRFilter) CoordinateFilter(gdsc.smlm.results.filter.CoordinateFilter) WidthFilter(gdsc.smlm.results.filter.WidthFilter) ANRFilter(gdsc.smlm.results.filter.ANRFilter) MultiHysteresisFilter(gdsc.smlm.results.filter.MultiHysteresisFilter) SNRFilter(gdsc.smlm.results.filter.SNRFilter) PrecisionHysteresisFilter(gdsc.smlm.results.filter.PrecisionHysteresisFilter) TraceFilter(gdsc.smlm.results.filter.TraceFilter) PrecisionFilter(gdsc.smlm.results.filter.PrecisionFilter) SNRHysteresisFilter(gdsc.smlm.results.filter.SNRHysteresisFilter) MemoryPeakResults(gdsc.smlm.results.MemoryPeakResults) ExtendedGenericDialog(ij.gui.ExtendedGenericDialog)

Example 15 with Filter

use of gdsc.smlm.results.filter.Filter in project GDSC-SMLM by aherbert.

the class FilterAnalysis method calculateSensitivity.

private void calculateSensitivity(List<MemoryPeakResults> resultsList) {
    if (!calculateSensitivity)
        return;
    if (!bestFilter.isEmpty()) {
        IJ.showStatus("Calculating sensitivity ...");
        createSensitivityWindow();
        int currentIndex = 0;
        for (String type : bestFilterOrder) {
            IJ.showProgress(currentIndex++, bestFilter.size());
            Filter filter = bestFilter.get(type).filter;
            ClassificationResult s = filter.score(resultsList);
            String message = type + "\t\t\t" + Utils.rounded(s.getJaccard(), 4) + "\t\t" + Utils.rounded(s.getPrecision(), 4) + "\t\t" + Utils.rounded(s.getRecall(), 4);
            if (isHeadless) {
                IJ.log(message);
            } else {
                sensitivityWindow.append(message);
            }
            // List all the parameters that can be adjusted.
            final int parameters = filter.getNumberOfParameters();
            for (int index = 0; index < parameters; index++) {
                // For each parameter compute as upward + downward delta and get the average gradient
                Filter higher = filter.adjustParameter(index, delta);
                Filter lower = filter.adjustParameter(index, -delta);
                ClassificationResult sHigher = higher.score(resultsList);
                ClassificationResult sLower = lower.score(resultsList);
                StringBuilder sb = new StringBuilder();
                sb.append("\t").append(filter.getParameterName(index)).append("\t");
                sb.append(Utils.rounded(filter.getParameterValue(index), 4)).append("\t");
                double dx1 = higher.getParameterValue(index) - filter.getParameterValue(index);
                double dx2 = filter.getParameterValue(index) - lower.getParameterValue(index);
                addSensitivityScore(sb, s.getJaccard(), sHigher.getJaccard(), sLower.getJaccard(), dx1, dx2);
                addSensitivityScore(sb, s.getPrecision(), sHigher.getPrecision(), sLower.getPrecision(), dx1, dx2);
                addSensitivityScore(sb, s.getRecall(), sHigher.getRecall(), sLower.getRecall(), dx1, dx2);
                if (isHeadless) {
                    IJ.log(sb.toString());
                } else {
                    sensitivityWindow.append(sb.toString());
                }
            }
        }
        String message = "-=-=-=-";
        if (isHeadless) {
            IJ.log(message);
        } else {
            sensitivityWindow.append(message);
        }
        IJ.showProgress(1);
        IJ.showStatus("");
    }
}
Also used : Filter(gdsc.smlm.results.filter.Filter) OrFilter(gdsc.smlm.results.filter.OrFilter) FilenameFilter(java.io.FilenameFilter) AndFilter(gdsc.smlm.results.filter.AndFilter) WidthFilter(gdsc.smlm.results.filter.WidthFilter) SNRFilter(gdsc.smlm.results.filter.SNRFilter) PrecisionHysteresisFilter(gdsc.smlm.results.filter.PrecisionHysteresisFilter) TraceFilter(gdsc.smlm.results.filter.TraceFilter) PrecisionFilter(gdsc.smlm.results.filter.PrecisionFilter) SNRHysteresisFilter(gdsc.smlm.results.filter.SNRHysteresisFilter) ClassificationResult(gdsc.core.match.ClassificationResult)

Aggregations

Filter (gdsc.smlm.results.filter.Filter)18 DirectFilter (gdsc.smlm.results.filter.DirectFilter)10 MaximaSpotFilter (gdsc.smlm.filters.MaximaSpotFilter)9 FilterSet (gdsc.smlm.results.filter.FilterSet)9 MultiPathFilter (gdsc.smlm.results.filter.MultiPathFilter)9 PrecisionFilter (gdsc.smlm.results.filter.PrecisionFilter)9 SNRFilter (gdsc.smlm.results.filter.SNRFilter)9 WidthFilter (gdsc.smlm.results.filter.WidthFilter)9 AndFilter (gdsc.smlm.results.filter.AndFilter)8 IDirectFilter (gdsc.smlm.results.filter.IDirectFilter)8 OrFilter (gdsc.smlm.results.filter.OrFilter)8 PrecisionHysteresisFilter (gdsc.smlm.results.filter.PrecisionHysteresisFilter)8 SNRHysteresisFilter (gdsc.smlm.results.filter.SNRHysteresisFilter)8 TraceFilter (gdsc.smlm.results.filter.TraceFilter)8 FilenameFilter (java.io.FilenameFilter)7 LinkedList (java.util.LinkedList)7 ArrayList (java.util.ArrayList)6 GenericDialog (ij.gui.GenericDialog)3 NonBlockingGenericDialog (ij.gui.NonBlockingGenericDialog)3 ClassificationResult (gdsc.core.match.ClassificationResult)2